LOCUS       Exported                8789 bp ds-DNA     circular SYN 02-OCT-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8789)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Dec 3, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8789
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     60..121
                     /label=1768a_Pum1_F
                     /note="color: black; sequence: 
                     GCTACTTGTTCTTTTTGCAGatggactacaaggacgatgacagcgtagcgtgtgtgttg
                     aag"
     CDS             80..100
                     /codon_start=1
                     /label=flag
                     /note="color: #339966"
                     /translation="MDYKDDD"
     CDS             101..3553
                     /codon_start=1
                     /label=pum1
                     /note="color: #ff9900"
                     /translation="SVACVLKRKAVLWQDSFSPHLRLPPPDRPLPSMPVVLSAAGHTPQ
                     PGPSPQAPAPTQGAGRSQDDAMVDYFFQRQHGDQPGYNNCKHRWPTGDNIHADNQVRSM
                     DELNHDFQALALEGRAMGEQLLTGKKFWETDDSGKDGPKGIFLDQWRDSAWGASDHSVS
                     QPIMVQRRPGQGFHGVGEAGSVLSPRSESGGLGVSMVEYVLSSSPAEKMDSCLRKGAYG
                     PRDSEADDPEKRVEQKPKTTFDPERKELKETEPDVLDNPNGLPNQNGLDVDVKDFSRTP
                     GNCPPTGQEVELGGPEMVGGAGPKPAEEFSNVETQNVTLDPMESVSMETLQFEYPGGQL
                     PLDSTAATVGLFDYNSPQQLFQRPNALAVQQLTAAQQQQYALAAAQQPHIGLAPAAFVP
                     NPYIISAAPPGTDPYAAGLAAAATLGPAVMPPQYYGVTPWGVYPANLFQQQAATPSNSA
                     NQQSAAQNQQNQQQVMRAGGNQRPLTPSQGQQGQQTEQLVAAAAVNSALAFGQGLAAGV
                     PGYPVLAPAAYYDQTGALVVNTGARGGPVRLMAPASVIISPSAAVAAAAASAGGANAGL
                     GGGASGAFRALSSQQAPGQQGSGALGGSSFYGSGSPSSSSQSSSLFSQGSAQPGNTSLG
                     FNANPTSSLGATLGAALGGFGTAVANSSSSGSRRDSLTGSSDLYKRTASSLTPIGHGAF
                     YNGLSFSSSPGPVGMPLPNQGPSHSLTPPPSLSTHGSSSSLNLGGLTNGSGRFISAAPG
                     AEAKYRSATTGSSLFSPSSQLFPSSRLRYGMSDVMPSGRSRLLEDFRNNRYPNLQLREI
                     AGHVMEFSQDQHGSRFIQLKLERASPAERQLVFNEILQAAYQLMVDVFGNYVIQKFFEF
                     GSLDQKLALAERIRGHVLSLALQMYGCRVIQKALEFIPSDQQVINEMVRELDGHVLKCV
                     KDQNGNHVVQKCIECVQPHALQFIIDAFKGQVFALSTHPYGCRVIQRILEHCLPEQTLS
                     ILEEIHQHTEQLVQDQYGNYVIQHVLEHGRAEDKSKIVSEIRGNVLGLSQHKFASNVVE
                     KCVTHSLRAERAMLIDEVCSMADGPHSALYTMMKDQYANYVVQKMIDVAEPTQRKIVMH
                     KIRPHIATLRKYTYGKHILAKLEKYYMKNGVDLGPLCAPPNGIM"
     CDS             2576..2590
                     /codon_start=1
                     /label=TRM Repeat 1
                     /note="color: #993366"
                     /translation="SRFIQ"
     CDS             2684..2698
                     /codon_start=1
                     /label=TRM Repeat 2
                     /note="color: #993366"
                     /translation="NYVIQ"
     CDS             2792..2806
                     /codon_start=1
                     /label=TRM Repeat 3
                     /note="color: #993366"
                     /translation="CRVIQ"
     CDS             2906..2920
                     /codon_start=1
                     /label=TRM Repeat 4
                     /note="color: #993366"
                     /translation="NHVVQ"
     CDS             3014..3028
                     /codon_start=1
                     /label=TRM Repeat 5
                     /note="color: #993366"
                     /translation="CRVIQ"
     primer_bind     complement(3025..3042)
                     /label=1770_Adar_f_for_pum1
                     /note="color: black; sequence: 
                     cccaatggcatcatgCCATACGATGTTCCAGATTACGctgg"
     primer_bind     complement(3025..3042)
                     /label=Primer 1
                     /note="color: black; sequence: 
                     GGGTTACGGAGGCTCGTATCCATACGATGTTCCAGATTACGctgg"
     CDS             3122..3136
                     /codon_start=1
                     /label=TRM Repeat 6
                     /note="color: #993366"
                     /translation="NYVIQ"
     CDS             3230..3244
                     /codon_start=1
                     /label=TRM Repeat 7
                     /note="color: #993366"
                     /translation="SNVVE"
     CDS             3359..3373
                     /codon_start=1
                     /label=TRM Repeat 8
                     /note="color: #993366"
                     /translation="NYVVQ"
     primer_bind     complement(3533..3553)
                     /label=1768b_Pum1_R
                     /note="color: black; sequence: 
                     GCTCGAGAGGCCTTGAATTCGAAttacatgatgccattgggaggagc"
     primer_bind     3539..3579
                     /label=1770_Adar_f_for_pum1
                     /note="color: black; sequence: 
                     cccaatggcatcatgCCATACGATGTTCCAGATTACGctgg"
     primer_bind     3554..3604
                     /label=1141a-HA-Adar-forw
                     /note="color: black; sequence: 
                     TACCCATACGATGTTCCAGATTACGCTggcggaCAGCTGGACCAAACACCGTCC"
     primer_bind     3554..3579
                     /label=Primer 1
                     /note="color: black; sequence: 
                     GGGTTACGGAGGCTCGTATCCATACGATGTTCCAGATTACGctgg"
     misc_feature    3554..3577
                     /label=HA
                     /note="color: #0000ff"
     primer_bind     3554..3576
                     /label=HA_forw_kozak
                     /note="color: black; sequence: 
                     GGATCCCATCGATTCGccATGTACCCATACGATGTTCCAGATTACGC"
     misc_feature    3584..4788
                     /label=Catalytic Site
                     /note="color: #ff6600; direction: RIGHT"
     CDS             3584..4788
                     /codon_start=1
                     /label=adarb1a
                     /note="color: #993366"
                     /translation="QLDQTPSRQPIPREGLQLHLPQVLADAVSRLVVDKFSELTDNFSS
                     PHARRKVLAGVVMTTGKDVMDAQVICVTTGTKCINGEYMSDRGLALNDCHAEIIARRSL
                     IRYLYNQLEYFLSDSTEEHEKSIFRWCSEHGYRLKDDIQFHLYISTSPCGDARIFSPHE
                     AGAEDQGDRHPNRKARGQLRTKIESGEGTIPVRTSNTIQTWDGVLQGERLLTMSCSDKI
                     ARWNVIGVQGSLLSYFTEPIYFSSLILGSLYHADHLSRAMYQRIADMDDIPPPFRINRP
                     LLSGISNTEARQPGKAPNFSVNWTVGDQGLEIINATTGKDDLGRQSQLCKHALYSRWVC
                     LHAKLSETLRIRGSRPGSYHEAKQGAVEYHEAKQTLFKAFYKAGLGAWVEKPIEQDQFS
                     LT"
     primer_bind     3584..3600
                     /label=1101a
                     /note="color: black; sequence: ATGCAGCTGGACCAAACACC"
     primer_bind     3584..3600
                     /label=1111a
                     /note="color: black; sequence: 
                     GGCCCCCCCTCGAGGATGCAGCTGGACCAAACACC"
     CDS             4152..4153
                     /label=E488Q
                     /note="color: #00ccff; direction: RIGHT"
     primer_bind     complement(4762..4813)
                     /label=1141b-Adar1-Rev
                     /note="color: black; sequence: 
                     CTAGAGGCTCGAGAGGCCTTGCTAGGGAGTAAGGGAAAACTGGTCTTGTTCG"
     primer_bind     complement(4765..4789)
                     /label=1111b
                     /note="color: black; sequence: 
                     gggtacatggtggcGGGAGTAAGGGAAAACTGGTCTTGT"
     primer_bind     complement(4765..4788)
                     /label=1101b
                     /note="color: black; sequence: AGGAGTAAGGGAAAACTGGTCTTGT"
     primer_bind     complement(4793..4807)
                     /label=1768b_Pum1_R
                     /note="color: black; sequence: 
                     GCTCGAGAGGCCTTGAATTCGAAttacatgatgccattgggaggagc"
     misc_feature    4811..4838
                     /label=stop codons
                     /note="stop codons in all three reading frames"
                     /note="color: #ff0000"
     primer_bind     complement(4835..4858)
                     /label=Primer 2
                     /note="color: black; sequence: GTATCTTATCATGTCTGGATCTAC"
     polyA_signal    4838..4972
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     promoter        complement(5085..5103)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(5124..5140)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    5148..5164
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(5172..5202)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 5172 .. 5178 / #ffffff / -10
                        2: 5179 .. 5196 / #ffffff
                        3: 5197 .. 5202 / #ffffff / -35"
     rep_origin      complement(5526..6114)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(6285..7145)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 6285 .. 7076 / #ccffcc
                        2: 7077 .. 7145 / #ccffcc / signal sequence
                     Cleavage site after base 7076"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7146..7250)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      complement(7276..7731)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        7805..8789
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
                     /note="color: #ffffff; direction: RIGHT"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcaga tggactacaa ggacgatgac agcgtagcgt gtgtgttgaa
      121 gaggaaagct gtgctctggc aggattcgtt cagcccccac ctgagactgc ctccccctga
      181 caggccatta cccagcatgc ctgtggtgct gagtgctgcg ggccacaccc ctcaacccgg
      241 cccttctcct caggcccctg ccccgacaca gggagccgga cgatcgcaag atgacgcaat
      301 ggtggattat ttctttcagc ggcagcacgg ggaccagccc ggatacaaca actgcaaaca
      361 ccgctggccg accggcgaca atattcatgc agacaaccag gtacgatcca tggatgagct
      421 aaatcatgac ttccaggcac tggcactgga gggtcgggcc atgggagagc agctccttac
      481 tgggaagaag ttttgggaaa ctgatgattc agggaaagat ggaccaaaag ggatctttct
      541 ggaccagtgg agagacagcg cttggggagc ctctgatcac tcagtgtctc agccaatcat
      601 ggtgcagcgc cgaccgggtc agggcttcca cggagtggga gaggcgggat ctgtgctgtc
      661 gccgcggtcc gagagcggtg ggttaggggt ttccatggtg gagtatgtgc tcagctcctc
      721 acctgctgag aagatggact cgtgtctgcg caaaggagcc tatggtccaa gagattcaga
      781 ggctgatgac cctgagaaaa gggtggaaca aaaacccaag acaacgtttg accccgaacg
      841 aaaggaactg aaggagaccg aaccagatgt tttggataat cctaatggac tgcccaatca
      901 gaatggactt gatgtggatg ttaaagattt tagtcggacc cctggaaatt gccctccaac
      961 tggtcaagag gtggagttgg gaggaccaga gatggtagga ggtgcagggc cgaaaccagc
     1021 cgaggagttt tcaaatgttg aaacccaaaa tgtgaccctg gaccccatgg agtcagtgtc
     1081 tatggagact ttgcagttcg agtaccctgg aggacagttg ccgcttgact ccacagctgc
     1141 taccgtcggc ctgtttgact acaactcccc tcagcagctg ttccagagac ccaatgctct
     1201 ggcagtccag cagctgaccg cagcccagca gcagcagtac gcgctcgccg cagcccaaca
     1261 gccacacatt ggtttggctc cagctgcatt tgtaccgaat ccgtacatca tcagcgctgc
     1321 tccgcccggc accgacccgt atgctgcagg actcgctgct gccgccactc taggcccagc
     1381 ggtgatgccc cctcagtact atggagtgac tccatgggga gtgtatcctg ccaacctgtt
     1441 ccagcagcag gctgccacgc catccaactc ggccaaccag cagtcagcag cgcagaacca
     1501 gcagaaccaa cagcaggtga tgcgtgcagg tggaaaccag cgacctctca ctccaagtca
     1561 aggacagcag ggtcagcaga cggagcagct cgtagcggca gcagctgtga actcggcatt
     1621 ggcttttggg cagggtctgg cagcaggtgt accaggttat ccagtgttgg ctcctgctgc
     1681 atattatgac cagacaggtg cacttgtggt gaatacagga gctcgtggag gacccgttcg
     1741 acttatggcc cccgcatcag taatcatcag cccatcagcc gcagtggctg ctgccgccgc
     1801 ttcagcagga ggggcgaatg caggtctcgg tggtggagcc agcggtgcgt ttcgtgcatt
     1861 gagttcccag caggctcctg ggcagcaagg gagcggggct ttgggaggaa gctcttttta
     1921 tgggagcgga tctccgagct cctcttcaca gagctcctcc ctgttctccc aaggctccgc
     1981 tcagccggga aacacctcac tcggattcaa tgccaaccca acatcatctc tcggagccac
     2041 gcttggagca gcactcgggg gttttggcac ggcagtggct aattccagtt ccagcgggtc
     2101 tcgtcgggat tctctgacgg gcagctctga tctgtacaag cggacagcca gcagcctcac
     2161 tcctattggc catggagcct tttacaacgg cctcagcttt agctcctccc ctggtccggt
     2221 tggcatgcca cttccaaacc aaggcccctc ccactctctc accccgccac cctcgctctc
     2281 aactcatgga tcctcaagca gtcttaatct tggtggactg accaatggca gtgggcgctt
     2341 catctcagca gcacctggag cggaggcgaa gtaccgcagc gccaccaccg gctcgtctct
     2401 ctttagtccc agcagtcagc tcttcccctc gtcacgtctg cgctacggga tgtctgacgt
     2461 gatgccgtcg ggacgcagcc gtctgctgga ggacttcaga aacaaccgct accccaacct
     2521 gcagctgaga gagatcgcag gacacgtcat ggagttctct caggaccagc acggctccag
     2581 gtttattcag ctgaaattgg agagagcaag tcctgcagag cgtcagctgg tcttcaatga
     2641 gatcctgcag gccgcctacc agctcatggt tgatgtgttt ggaaattatg tcatccagaa
     2701 gttctttgag tttggcagtc tggatcagaa gctggcgctg gccgagcgga ttcgtggtca
     2761 tgtgttatcc ctggcgctgc agatgtacgg ctgcagagtc attcagaaag ccctggagtt
     2821 catcccctcc gaccagcaag tcattaatga gatggttcgg gagctcgacg gacacgtgtt
     2881 aaagtgtgtg aaggatcaaa acggcaatca tgtggtgcag aaatgcattg agtgtgttca
     2941 gcctcatgct cttcagttca tcattgatgc cttcaaagga caggtgtttg ctctgtccac
     3001 acacccatat ggctgtcggg tgatccagcg tattctggag cactgcctgc ccgagcagac
     3061 tttatccatc ctggaggaaa tccatcagca cactgagcag ctggtgcagg atcagtatgg
     3121 gaattatgta atccagcacg tgttggagca cggccgcgct gaggacaaga gcaagattgt
     3181 atctgaaatc cgaggaaatg ttttggggct cagccagcat aagtttgcca gtaatgtggt
     3241 ggagaagtgt gtgacccact ctctgcgtgc cgaacgggcc atgctgatcg atgaggtgtg
     3301 cagtatggcg gacgggccac acagtgcctt atacaccatg atgaaggacc agtacgcaaa
     3361 ctatgttgtg cagaagatga tcgatgtggc cgagccaaca caacgaaaga ttgtaatgca
     3421 caagatccgg ccccatattg cgacgctgag gaagtacacg tacggaaagc acattttggc
     3481 caagctggag aagtactaca tgaagaacgg tgttgacctg ggcccgctct gcgctcctcc
     3541 caatggcatc atgccatacg atgttccaga ttacgctggc ggacagctgg accaaacacc
     3601 gtcccgacag ccaatcccca gggagggact tcagctgcat ttgccccagg tcctcgcgga
     3661 tgcggtctct cgtttagttg tggataagtt cagcgagctg acagacaact tctcgtctcc
     3721 acatgcacgg cggaaagtcc tggcaggagt tgtcatgaca accggtaagg atgtgatgga
     3781 cgctcaggtg atttgtgtga cgacgggaac caagtgtata aacggagagt acatgagcga
     3841 tcgaggactg gcccttaatg actgccatgc tgagatcatc gcacggcgct cactcattag
     3901 atacctgtac aatcagctgg aatacttcct cagcgacagc actgaagaac atgagaaatc
     3961 catattcaga tggtgcagtg aacatggcta ccggctaaaa gatgatattc aattccatct
     4021 gtatatcagc acgtcgccct gtggagacgc ccggatcttc tcccctcatg aggctggtgc
     4081 cgaggaccag ggtgaccgac atccaaaccg caaggcccgt ggacaactac gaaccaagat
     4141 tgagtctgga gaaggaacga tacctgttcg tacgagtaac accattcaga cttgggatgg
     4201 agtcctgcag ggagagcgac tgctaaccat gtcatgcagt gacaaaattg ccagatggaa
     4261 cgtgatcgga gtacagggat ctcttctgag ttacttcaca gagcccatct atttctccag
     4321 tttaattctg ggaagtctgt atcatgctga ccatctttcc agagccatgt accagcgcat
     4381 cgcagacatg gatgatattc ccccgccatt cagaatcaac agaccacttc ttagcggtat
     4441 cagtaacaca gaagcaaggc aacctgggaa agctcctaat ttcagcgtca actggacagt
     4501 aggagaccag gggttggaga tcattaacgc aaccacagga aaagatgacc tcggaagaca
     4561 gtcgcaactt tgcaaacacg ctctttacag tcgttgggtc tgtctacatg caaagctcag
     4621 tgagactctg cggatccgag gatctcggcc tggctcctac cacgaggcaa aacaaggggc
     4681 ggtcgagtat catgaggcga aacagactct tttcaaagct ttctacaaag cgggactggg
     4741 agcatgggtg gaaaaaccca tcgaacaaga ccagttttcc cttactccct agcaaggcct
     4801 ctcgagcctc tagaactata gtgagtcgta ttacgtagat ccagacatga taagatacat
     4861 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     4921 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     4981 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaattcgc
     5041 ggccgcggcg ccaatgcatt gggcccggta cccagctttt gttcccttta gtgagggtta
     5101 attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     5161 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     5221 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     5281 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     5341 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     5401 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     5461 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     5521 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     5581 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     5641 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     5701 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     5761 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     5821 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     5881 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     5941 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     6001 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     6061 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     6121 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     6181 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     6241 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     6301 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     6361 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     6421 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     6481 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     6541 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     6601 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     6661 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     6721 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     6781 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     6841 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     6901 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     6961 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     7021 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     7081 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     7141 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     7201 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     7261 cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     7321 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     7381 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     7441 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     7501 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     7561 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     7621 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     7681 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc
     7741 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     7801 agtcgaccat agccaattca atatggcgta tatggactca tgccaattca atatggtgga
     7861 tctggacctg tgccaattca atatggcgta tatggactcg tgccaattca atatggtgga
     7921 tctggacccc agccaattca atatggcgga cttggcacca tgccaattca atatggcgga
     7981 cttggcactg tgccaactgg ggaggggtct acttggcacg gtgccaagtt tgaggagggg
     8041 tcttggccct gtgccaagtc cgccatattg aattggcatg gtgccaataa tggcggccat
     8101 attggctata tgccaggatc aatatatagg caatatccaa tatggcccta tgccaatatg
     8161 gctattggcc aggttcaata ctatgtattg gccctatgcc atatagtatt ccatatatgg
     8221 gttttcctat tgacgtagat agcccctccc aatgggcggt cccatatacc atatatgggg
     8281 cttcctaata ccgcccatag ccactccccc attgacgtca atggtctcta tatatggtct
     8341 ttcctattga cgtcatatgg gcggtcctat tgacgtatat ggcgcctccc ccattgacgt
     8401 caattacggt aaatggcccg cctggctcaa tgcccattga cgtcaatagg accacccacc
     8461 attgacgtca atgggatggc tcattgccca ttcatatccg ttctcacgcc ccctattgac
     8521 gtcaatgacg gtaaatggcc cacttggcag tacatcaata tctattaata gtaacttggc
     8581 aagtacatta ctattggaag gacgccaggg tacattggca gtactcccat tgacgtcaat
     8641 ggcggtaaat ggcccgcgat ggctgccaag tacatcccca ttgacgtcaa tggggagggg
     8701 caatgacgca aatgggcgtt ccattgacgt aaatgggcgg taggcgtgcc taatgggagg
     8761 tctatataag caatgctcgt ttagggaac
//
