LOCUS       Exported                5361 bp ds-DNA     circular SYN 14-DEC-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5361)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Dec 3, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5361
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     77..110
                     /label=2138a_N22
                     /note="color: black; sequence: 
                     CAGGATCCCATCGATTCGCGccATGgacgcgcag"
     misc_feature    79..96
                     /label=pCS2
                     /note="color: #a6acb3"
     misc_feature    95..102
                     /label=kozak
                     /note="color: #339966"
     CDS             99..164
                     /codon_start=1
                     /label=lambda N22 peptide
                     /note="color: #ffcc00"
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     misc_feature    165..191
                     /label=HA
                     /note="color: #0000ff"
     primer_bind     177..225
                     /label=2140
                     /note="color: black; sequence: 
                     GTTCCAGATTACGCTggatatctcggaggccagctgcatttaccgcagg"
     primer_bind     complement(177..198)
                     /label=2138b
                     /note="color: black; sequence: gatatccAGCGTAATCTGGAAC"
     CDS             201..1364
                     /codon_start=1
                     /label=hsADAR2_E488Q_T375G
                     /note="color: #99cc00"
                     /translation="GGQLHLPQVLADAVSRLVLGKFGDLTDNFSSPHARRKVLAGVVMT
                     TGTDVKDAKVISVSTGGKCINGEYMSDRGLALNDCHAEIISRRSLLRFLYTQLELYLNN
                     KDDQKRSIFQKSERGGFRLKENVQFHLYISTSPCGDARIFSPHEPILEEPADRHPNRKA
                     RGQLRTKIESGQGTIPVRSNASIQTWDGVLQGERLLTMSCSDKIARWNVVGIQGSLLSI
                     FVEPIYFSSIILGSLYHGDHLSRAMYQRISNIEDLPPLYTLNKPLLSGISNAEARQPGK
                     APNFSVNWTVGDSAIEVINATTGKDELGRASRLCKHALYCRWMRVHGKVPSHLLRSKIT
                     KPNVYHESKLAAKEYQAAKARLFTAFIKAGLGAWVEKSTEQDQFSLT"
     primer_bind     complement(1341..1380)
                     /label=2093b_hsAdar2_Rev_EcorI
                     /note="color: black; sequence: 
                     GGCTCGAGAGGCCTTGttacgtgagtgagaactggtcctg"
     primer_bind     1373..1399
                     /label=rev_site
                     /note="color: black; sequence: 
                     CACTTATTCGTCCAACTCGAGCCTCTAGAACTATAGTGAGTC"
     misc_feature    1383..1410
                     /label=stop codons
                     /note="stop codons in all three reading frames"
                     /note="color: #ff0000"
     polyA_signal    1410..1544
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(1432..1453)
                     /label=1017b
                     /note="color: black; sequence: GTGGTTTGTCCAAACTCATCAA"
     primer_bind     1614..1625
                     /label=Primer 1
                     /note="color: black; sequence: 
                     GAAcTCCAGTCACtacGCCGCGGCGCCA"
     promoter        complement(1657..1675)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(1676..1688)
                     /label=Primer 2
                     /note="color: black; sequence: 
                     CTGGATCTACtacGCGGCCGCACGCCAAGCGCGC"
     primer_bind     complement(1696..1712)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    1720..1736
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(1744..1774)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 1744 .. 1750 / #ffffff / -10
                        2: 1751 .. 1768 / #ffffff
                        3: 1769 .. 1774 / #ffffff / -35"
     rep_origin      complement(2098..2686)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(2857..3717)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 2857 .. 3648 / #ccffcc
                        2: 3649 .. 3717 / #ccffcc / signal sequence
                     Cleavage site after base 3648"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3718..3822)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      complement(3848..4303)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        4377..5361
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     join(5349..5361,1..9)
                     /label=1017a
                     /note="color: black; sequence: TCGTTTAGGGAACCGCCATTCT"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgcgccat ggacgcgcag acgcggcgac
      121 gcgagcgccg ggcagagaag caggctcaat ggaaggcagc taactaccca tacgatgttc
      181 cagattacgc tggatatctc ggaggccagc tgcatttacc gcaggtttta gctgacgctg
      241 tctcacgcct ggtcctgggt aagtttggtg acctgaccga caacttctcc tcccctcacg
      301 ctcgcagaaa agtgctggct ggagtcgtca tgacaacagg cacagatgtt aaagatgcca
      361 aggtgataag tgtttctaca ggaggcaaat gtattaatgg tgaatacatg agtgatcgtg
      421 gccttgcatt aaatgactgc catgcagaaa taatatctcg gagatccttg ctcagatttc
      481 tttatacaca acttgagctt tacttaaata acaaagatga tcaaaaaaga tccatctttc
      541 agaaatcaga gcgagggggg tttaggctga aggagaatgt ccagtttcat ctgtacatca
      601 gcacctctcc ctgtggagat gccagaatct tctcaccaca tgagccaatc ctggaagaac
      661 cagcagatag acacccaaat cgtaaagcaa gaggacagct acggaccaaa atagagtctg
      721 gtcaggggac gattccagtg cgctccaatg cgagcatcca aacgtgggac ggggtgctgc
      781 aaggggagcg gctgctcacc atgtcctgca gtgacaagat tgcacgctgg aacgtggtgg
      841 gcatccaggg atcactgctc agcattttcg tggagcccat ttacttctcg agcatcatcc
      901 tgggcagcct ttaccacggg gaccaccttt ccagggccat gtaccagcgg atctccaaca
      961 tagaggacct gccacctctc tacaccctca acaagccttt gctcagtggc atcagcaatg
     1021 cagaagcacg gcagccaggg aaggccccca acttcagtgt caactggacg gtaggcgact
     1081 ccgctattga ggtcatcaac gccacgactg ggaaggatga gctgggccgc gcgtcccgcc
     1141 tgtgtaagca cgcgttgtac tgtcgctgga tgcgtgtgca cggcaaggtt ccctcccact
     1201 tactacgctc caagattacc aagcccaacg tgtaccatga gtccaagctg gcggcaaagg
     1261 agtaccaggc cgccaaggcg cgtctgttca cagccttcat caaggcgggg ctgggggcct
     1321 gggtggagaa gtccaccgag caggaccagt tctcactcac gtaacaaggc ctctcgagcc
     1381 tctagaacta tagtgagtcg tattacgtag atccagacat gataagatac attgatgagt
     1441 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     1501 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     1561 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaattc gcggccgcgg
     1621 cgccaatgca ttgggcccgg tacccagctt ttgttccctt tagtgagggt taattgcgcg
     1681 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     1741 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     1801 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     1861 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     1921 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     1981 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     2041 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     2101 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     2161 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     2221 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     2281 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     2341 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     2401 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     2461 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     2521 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     2581 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     2641 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     2701 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     2761 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     2821 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     2881 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     2941 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     3001 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     3061 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     3121 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     3181 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     3241 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     3301 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     3361 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     3421 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     3481 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     3541 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     3601 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     3661 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     3721 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     3781 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     3841 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     3901 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     3961 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     4021 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     4081 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     4141 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     4201 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     4261 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg
     4321 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagtcgacc
     4381 atagccaatt caatatggcg tatatggact catgccaatt caatatggtg gatctggacc
     4441 tgtgccaatt caatatggcg tatatggact cgtgccaatt caatatggtg gatctggacc
     4501 ccagccaatt caatatggcg gacttggcac catgccaatt caatatggcg gacttggcac
     4561 tgtgccaact ggggaggggt ctacttggca cggtgccaag tttgaggagg ggtcttggcc
     4621 ctgtgccaag tccgccatat tgaattggca tggtgccaat aatggcggcc atattggcta
     4681 tatgccagga tcaatatata ggcaatatcc aatatggccc tatgccaata tggctattgg
     4741 ccaggttcaa tactatgtat tggccctatg ccatatagta ttccatatat gggttttcct
     4801 attgacgtag atagcccctc ccaatgggcg gtcccatata ccatatatgg ggcttcctaa
     4861 taccgcccat agccactccc ccattgacgt caatggtctc tatatatggt ctttcctatt
     4921 gacgtcatat gggcggtcct attgacgtat atggcgcctc ccccattgac gtcaattacg
     4981 gtaaatggcc cgcctggctc aatgcccatt gacgtcaata ggaccaccca ccattgacgt
     5041 caatgggatg gctcattgcc cattcatatc cgttctcacg ccccctattg acgtcaatga
     5101 cggtaaatgg cccacttggc agtacatcaa tatctattaa tagtaacttg gcaagtacat
     5161 tactattgga aggacgccag ggtacattgg cagtactccc attgacgtca atggcggtaa
     5221 atggcccgcg atggctgcca agtacatccc cattgacgtc aatggggagg ggcaatgacg
     5281 caaatgggcg ttccattgac gtaaatgggc ggtaggcgtg cctaatggga ggtctatata
     5341 agcaatgctc gtttagggaa c
//
