LOCUS       pEM102_mClover3-       11955 bp    DNA     circular SYN 06-DEC-2025
DEFINITION  EM102.
ACCESSION   EM102
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 6, 2025 from SnapGene 8.0.3
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..11955
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=high-copy-number ColE1/pMB1/pBR322/pUC 
                     origin
                     of replication "
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     repeat_region   complement(749..760)
                     /label=AAV2 ITR (fragment)
                     /note="pLannotate"
                     /note="/database=itrs"
                     /note="/description=AAV2 ITR Flip"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.3"
                     /note="/other=repeat_region"
     misc_feature    complement(775..915)
                     /label=bom
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=basis of mobility region from pBR322"
                     /note="/fragment=False"
                     /note="/identity=99.3"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             complement(1017..1056)
                     /codon_start=1
                     /label=rop (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=Rop protein, which maintains plasmids 
                     at low
                     copy number; rop"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=20.8"
                     /note="/other=CDS"
                     /translation="LPRAFR**R*KPL"
     rep_origin      complement(1259..1453)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=origin of replication for the 
                     Pseudomonas
                     plasmid pVS1 (Heeb et al., 2000)"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(1519..2592)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=replication protein from the 
                     Pseudomonas
                     plasmid pVS1 (Heeb et al., 2000)"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="VSGRKPSGPVQTGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3021..3650)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=stability protein from the Pseudomonas
                     plasmid pVS1 (Heeb et al., 2000)"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             complement(4210..4611)
                     /codon_start=1
                     /label=Y4CG_SINFN (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/description=Protein inferred from homology: 
                     Swiss-Prot
                     protein existence level 3. From Sinorhizobium fredii 
                     (strain NBRC 101917 / NGR234)."
                     /note="/fragment=True"
                     /note="/identity=51.5"
                     /note="/match_length=43.9"
                     /note="/other=CDS"
                     /translation="LLGYARVSTDDQDLTNQRAELHAAGCTKLFSEKITGTRRDRPELA
                     RMLDHLRPGDVVTVTRLDRLARSTRDLLDIAERIQEAGAGLRSLAEPWADTTTPAGRMV
                     LTVFAGIAEFERSLIIDRTRSGREAAKARG"
     misc_feature    complement(4951..4975)
                     /label=RB T-DNA repeat
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=right border repeat from nopaline C58 
                     T-DNA"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             complement(5041..5073)
                     /codon_start=1
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=LacZ-alpha fragment of 
                     beta-galactosidase; lacZ
                     fragment"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=19.0"
                     /note="/other=CDS"
                     /translation="HWPSFYNVVTG"
     primer_bind     5056..5072
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5088..5260
                     /label=alpha-Tub_promoter_SPPG_05136
     misc_feature    5261..5419
                     /label=5UTR_alpha-Tub
     CDS             5426..6142
                     /codon_start=1
                     /label=mClover3
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHYVYITADKQKNCIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             6170..7516
                     /codon_start=1
                     /label=Bden_alpha-Tub_BDEG_00078
                     /translation="RECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGD
                     DSFNTFFSETGAGKHVPRTVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYAR
                     GHYTIGKEIVDLVLDRIRKLADNCTGLQGFLVFHSFGGGTGSGFGALLLERLSVDYGKK
                     SKLEFSVYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPT
                     YTNLNRLIAQVVSSITASLRFDGSLNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAY
                     HEQLTVAEITYSCFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTI
                     QFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYA
                     KRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGTDSVEGEEEEAEEY"
     misc_feature    7520..7770
                     /label=Bd-alpha-Tub_3UTR
     misc_feature    7777..7993
                     /label=SpunH2ABpr
     misc_feature    7994..8059
                     /label=5UTR_H2B
     CDS             8078..8134
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8144..8197
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8216..9244
                     /codon_start=1
                     /label=HygR
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     misc_feature    9254..9302
                     /label=SynTher8
     primer_bind     complement(9337..9353)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(9428..9452)
                     /label=LB T-DNA repeat
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=left border repeat from nopaline C58 
                     T-DNA"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             9876..10670
                     /codon_start=1
                     /label=KanR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=aminoglycoside phosphotransferase; 
                     aphA-3;
                     confers resistance to kanamycin"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    complement(10678..11872)
                     /label=E. Coli IS5 Transposon
                     /note="pLannotate"
                     /note="/database=is_db"
                     /note="/description=Transposon IS5 from E. Coli"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=transposon"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggcttcgcc acctctgact
      541 tgagcgtcga cttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      841 tacaatctgc tctgacgccg catagttaag ccagtataca ctccgctatc gctacgtgac
      901 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
      961 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     1021 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg
     1081 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
     1141 catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
     1201 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
     1261 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
     1321 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
     1381 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
     1441 cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc
     1501 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
     1561 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
     1621 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
     1681 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
     1741 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
     1801 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
     1861 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
     1921 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
     1981 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
     2041 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
     2101 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
     2161 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
     2221 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
     2281 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
     2341 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
     2401 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
     2461 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
     2521 caacttctcc accaggtcat cacccagcgc cgcgccggtt tgtaccgggc cggatggttt
     2581 gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
     2641 gcaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
     2701 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
     2761 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
     2821 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
     2881 cggcaactga aagttgaccc gcttcacggc tggcgtgtct gccaggctgg ccaacgttgc
     2941 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
     3001 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
     3061 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
     3121 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
     3181 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
     3241 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
     3301 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
     3361 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
     3421 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
     3481 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
     3541 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
     3601 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
     3661 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
     3721 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
     3781 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
     3841 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
     3901 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
     3961 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg
     4021 gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg
     4081 gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc
     4141 ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc
     4201 aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag
     4261 ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt
     4321 ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
     4381 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
     4441 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
     4501 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
     4561 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
     4621 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
     4681 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
     4741 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
     4801 tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg
     4861 ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg
     4921 attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac
     4981 tgatagttta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
     5041 cccagtcacg acgttgtaaa acgacggcca gtgccagatc tgttaaccca catatcatac
     5101 gtgtttgtcc gtctggccct gttcggacat aatttgtact ttcctaataa agctaatgaa
     5161 tatccatata aggcaactgc atggctagtg tgaacgcccc ctttgtgcca ttcaatttgg
     5221 agacttataa aaaggacgtc cgctcgcccc cccggacgtg attccatctc accttttgtt
     5281 tgccaaccaa atcgtttaca caggtcttct tcatcgcctg aagaccgaat aacctttttt
     5341 tgtttggcac tcgactctct tgcttgcaat caaaccctcc actcctctct cttttttgtt
     5401 tttttgagaa tctataaccc ttaagatggt gagcaagggc gaggagctgt tcaccggggt
     5461 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtccgcgg
     5521 cgagggcgag ggcgatgcca ccaacggcaa gctgaccctg aagttcatct gcaccaccgg
     5581 caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcg tggcctgctt
     5641 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     5701 ctacgtccag gagcgcacca tctctttcaa ggacgacggt acctacaaga cccgcgccga
     5761 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     5821 ggaggacggc aacatcctgg ggcacaagct ggagtacaac ttcaacagcc actacgtcta
     5881 tatcacggcc gacaagcaga agaactgcat caaggctaac ttcaagatcc gccacaacgt
     5941 tgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     6001 ccccgtgctg ctgcccgaca accactacct gagccatcag tccaagctga gcaaagaccc
     6061 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggattacaca
     6121 tggcatggac gagctgtaca agggatcctc cggactcaga tctcgagtgc gtgagtgtat
     6181 ctctatccat gtcggccagg ccggtgttca aattggtaat gcctgctggg agctgtactg
     6241 tctcgagcac ggcatccagc ctgatggtca gatgccctcg gacaagacca ttggtggtgg
     6301 cgacgactcc ttcaacacct tcttctctga gactggtgct ggaaagcacg ttccccgtac
     6361 tgtgtttgtc gaccttgagc caactgttgt tgatgaggtc cgtactggca cttatcgtca
     6421 gctgttccat cctgagcagc tgattaccgg aaaggaagat gctgccaaca actatgctcg
     6481 tggtcactac accattggca aggaaatcgt agatctggtt ctcgatcgta tccgaaaact
     6541 tgccgacaac tgtactggtc tgcaaggttt ccttgtcttc cactcctttg gtggtggtac
     6601 tggttctggt tttggtgctc tgttgctcga gcgtctttct gtcgactatg gcaaaaagtc
     6661 caagctggag ttttccgtgt atcccgctcc tcaagtgtcc actgctgttg ttgagcccta
     6721 caactctatc ctgaccactc acaccaccct tgagcactct gactgtgcct tcatggttga
     6781 taacgaagcc atctacgata tctgtcgccg caaccttgac attgagcgtc ccacttatac
     6841 caatctcaat cgtttgattg ctcaggttgt gtcgtccatc actgcttcgc ttcgttttga
     6901 tggttctctc aatgtcgatt tgaccgagtt ccagaccaac ttggttccct atccccgtat
     6961 ccatttccca ctggttacct atgctcccgt catttccgcc gagaaggctt atcacgagca
     7021 acttactgtt gccgagatta cctactcgtg cttcgagcct gctaaccaga tggttaaatg
     7081 tgatccccgt cacggaaaat acatggcttg ctgtcttctt taccgtggtg atgttgtccc
     7141 caaggacgtc aatgctgcca ttgccaccat caagaccaag cgtaccattc agtttgttga
     7201 ttggtgtcca actggtttca aggttggtat caactaccag ccacctactg ttgttcctgg
     7261 tggtgatctt gccaaggttc agcgtgctgt ttgcatgttg tccaacacca ccgccattgc
     7321 cgaagcatgg gctcgtctcg atcacaagtt cgatctcatg tacgccaagc gtgcctttgt
     7381 tcactggtat gttggtgaag gtatggaaga gggtgaattt tccgaggctc gtgaggatct
     7441 ggctgctctt gagaaggact acgaggaggt tggtaccgac tctgttgagg gtgaggaaga
     7501 agaggctgag gagtattaga tcaatcatag tacctcctct cttgattgca gcaagggtta
     7561 gaggacctcc tttctctgta taaaatttgt tcttgaataa gaggacgtga taccagttac
     7621 attcttctct gcttttaatt ccaaatgcct accccccgga atcatggaca cgttcaatga
     7681 tttaccaata gatgtatgca ttttgaaggg aataagtgtt ttggtatgag gcgaattata
     7741 gctgctagat caccattggc ataccaattg cctaggtttt atgctccaag cggagacgat
     7801 gaggccgata ttacagtggc ttagtagcgt gtacaatatt tccataaaag gctacatggt
     7861 gagtttttca cgtgaccgta attagtccca attaggcttt attgtatatt gctactaagc
     7921 atcgttaaat gacctgctcg aagcttcgtc tataaaaggc gggcgtgtgt gcgcttcccc
     7981 cgctacgtct ttttcacaaa cactttgcga cttgaccaag acctttcacc cgcaccactc
     8041 tcatccatca cacacacaag tcgacatggg aagcggagct actaacttca gcctgctgaa
     8101 gcaggctgga gacgtggagg agaaccctgg acctggaagc ggagagggca gaggaagtct
     8161 gctaacatgc ggtgacgtcg aggagaatcc tggacctacc gagctcggat ctaccatggg
     8221 taaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag
     8281 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt
     8341 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg
     8401 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg
     8461 ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca
     8521 agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc
     8581 gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat
     8641 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca
     8701 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct
     8761 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc
     8821 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat
     8881 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg
     8941 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg
     9001 gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg
     9061 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc
     9121 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg
     9181 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga
     9241 ataatggcgc gcctatataa actcatttac ttatgtagga ataaagagta tcatctttca
     9301 aagagctcga tatcttaatt aagaattcgt aatcatgtca tagctgtttc ctgtgtgaaa
     9361 attcggcgtt aattcagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg
     9421 tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc
     9481 agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg
     9541 ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg
     9601 caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta
     9661 acgatgacag agcgttgctg cctgtgatca ccgcggtttc aaaatcggct ccgtcgatac
     9721 tatgttatac gccaactttg aaaacaactt tgaaaagctg ttttctggta tttaaggttt
     9781 tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc
     9841 tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg
     9901 aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg
     9961 ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc
    10021 ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag
    10081 gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc
    10141 tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg
    10201 aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg
    10261 attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata
    10321 acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc
    10381 gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg
    10441 gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc
    10501 ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca
    10561 gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc
    10621 ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctaggga
    10681 aggtgcgaat aagcggggaa attcttctcg gctgactcag tcatttcatt tcttcatgtt
    10741 tgagccgatt ttttctcccg taaatgcctt gaatcagcct atttagaccg tttcttcgcc
    10801 atttaaggcg ttatccccag tttttagtga gatctctccc actgacgtat catttggtcc
    10861 gcccgaaaca ggttggccag cgtgaataac atcgccagtt ggttatcgtt tttcagcaac
    10921 cccttgtatc tggctttcac gaagccgaac tgtcgcttga tgatgcgaaa tgggtgctcc
    10981 accctggccc ggatgctggc tttcatgtat tcgatgttga tggccgtttt gttcttgcgt
    11041 ggatgctgtt tcaaggttct taccttgccg gggcgctcgg cgatcagcca gtccacatcc
    11101 acctcggcca gctcctcgcg ctgtggcgcc ccttggtagc cggcatcggc tgagacaaat
    11161 tgctcctctc catgcagcag attacccagc tgattgaggt catgctcgtt ggccgcggtg
    11221 gtgaccaggc tgtgggtcag gccactcttg gcatcgacac caatgtgggc cttcatgcca
    11281 aagtgccact gattgccttt cttggtctga tgcatctccg gatcgcgttg ctgctctttg
    11341 ttcttggtcg agctgggtgc ctcaatgatg gtggcatcga ccaaggtgcc ttgagtcatc
    11401 atgacgcctg cttcggccag ccagcgattg atggtcttga acaattggcg ggccagttga
    11461 tgctgctcca gcaggtggcg gaaattcatg atggtggtgc ggtccggcaa ggcgctatcc
    11521 agggataacc gggcaaacag acgcatggag gcgatttcgt acagagcatc ttccatcgcg
    11581 ccatcgctca ggttgtacca atgctgcatg cagtgaatgc gtagcatggt ttccagcgga
    11641 taaggtcgcc ggccattacc agccttgggg taaaacggct cgatgacttc caccatgttt
    11701 tgccatggca gaatctgctc catgcgggac aagaaaatct cttttctggt ctgacggcgc
    11761 ttactgctga attcactgtc ggcgaaggta agttgatgac tcatgatgaa ccctgttcta
    11821 tggctccaga tgacaaacat gatctcatat cagggacttg ttcgcacctt ccctagaatg
    11881 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    11941 gatcaaagga tcttc
//
