LOCUS       EM110_MLRC-mClov       10375 bp    DNA     circular SYN 06-DEC-2025
DEFINITION  EM110.
ACCESSION   EM110
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 6, 2025 from SnapGene 8.0.3
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
COMMENT     Sequence Label: pEM110_MLRC-mClover3
FEATURES             Location/Qualifiers
     source          1..10375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=high-copy-number ColE1/pMB1/pBR322/pUC 
                     origin
                     of replication "
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     repeat_region   complement(749..760)
                     /label=AAV2 ITR (fragment)
                     /note="pLannotate"
                     /note="/database=itrs"
                     /note="/description=AAV2 ITR Flip"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.3"
                     /note="/other=repeat_region"
     misc_feature    complement(775..915)
                     /label=bom
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=basis of mobility region from pBR322"
                     /note="/fragment=False"
                     /note="/identity=99.3"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             complement(1017..1056)
                     /codon_start=1
                     /label=rop (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=Rop protein, which maintains plasmids 
                     at low
                     copy number; rop"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=20.8"
                     /note="/other=CDS"
                     /translation="LPRAFR**R*KPL"
     rep_origin      complement(1259..1453)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=origin of replication for the 
                     Pseudomonas
                     plasmid pVS1 (Heeb et al., 2000)"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(1519..2592)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=replication protein from the 
                     Pseudomonas
                     plasmid pVS1 (Heeb et al., 2000)"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="VSGRKPSGPVQTGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3021..3650)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=stability protein from the Pseudomonas
                     plasmid pVS1 (Heeb et al., 2000)"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             complement(4210..4611)
                     /codon_start=1
                     /label=Y4CG_SINFN (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/description=Protein inferred from homology: 
                     Swiss-Prot
                     protein existence level 3. From Sinorhizobium fredii 
                     (strain NBRC 101917 / NGR234)."
                     /note="/fragment=True"
                     /note="/identity=51.5"
                     /note="/match_length=43.9"
                     /note="/other=CDS"
                     /translation="LLGYARVSTDDQDLTNQRAELHAAGCTKLFSEKITGTRRDRPELA
                     RMLDHLRPGDVVTVTRLDRLARSTRDLLDIAERIQEAGAGLRSLAEPWADTTTPAGRMV
                     LTVFAGIAEFERSLIIDRTRSGREAAKARG"
     misc_feature    complement(4951..4975)
                     /label=RB T-DNA repeat
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=right border repeat from nopaline C58 
                     T-DNA"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             complement(5041..5073)
                     /codon_start=1
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=LacZ-alpha fragment of 
                     beta-galactosidase; lacZ
                     fragment"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=19.0"
                     /note="/other=CDS"
                     /translation="HWPSFYNVVTG"
     primer_bind     5056..5072
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(5082..5240)
                     /label=3UTR_MLRC
     CDS             complement(5241..5264)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(5265..5981)
                     /codon_start=1
                     /label=mClover3
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHYVYITADKQKNCIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             complement(5997..6488)
                     /codon_start=1
                     /label=SpunMRLC_SPPG_07268
                     /translation="MSARAQKKKKRAVRQNSNVFAMFDQKQIAEFKEAFSMIDHDSDGF
                     IDKEDLKDMLASLGQQPTESSIDDMISEAPGSINFTMFLTLMGEKMSGTDPEHEILAAF
                     ECFDDNKSGVINAEVLRDYMTTMGDRFTDEEVDIMFKGAPVGANGQFNYREWVRVLKHG
                     E"
     misc_feature    complement(6495..6612)
                     /label=5UTR_H2A
     misc_feature    6619..6835
                     /label=H2ABpr
     misc_feature    6836..6901
                     /label=5UTR_H2B
     CDS             6920..6976
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6986..7039
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7058..8086
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     misc_feature    8096..8144
                     /label=SynTher8
     primer_bind     complement(8179..8195)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(8270..8294)
                     /label=LB T-DNA repeat
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=left border repeat from nopaline C58 
                     T-DNA"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     misc_feature    8727..9494
                     /label=E. Coli IS1A Transposon
                     /note="pLannotate"
                     /note="/database=is_db"
                     /note="/description=Transposon IS1A from E. Coli"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=transposon"
     CDS             9495..10289
                     /codon_start=1
                     /label=KanR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=aminoglycoside phosphotransferase; 
                     aphA-3;
                     confers resistance to kanamycin"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggcttcgcc acctctgact
      541 tgagcgtcga cttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      841 tacaatctgc tctgacgccg catagttaag ccagtataca ctccgctatc gctacgtgac
      901 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
      961 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     1021 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg
     1081 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
     1141 catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
     1201 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
     1261 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
     1321 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
     1381 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
     1441 cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc
     1501 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
     1561 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
     1621 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
     1681 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
     1741 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
     1801 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
     1861 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
     1921 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
     1981 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
     2041 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
     2101 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
     2161 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
     2221 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
     2281 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
     2341 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
     2401 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
     2461 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
     2521 caacttctcc accaggtcat cacccagcgc cgcgccggtt tgtaccgggc cggatggttt
     2581 gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
     2641 gcaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
     2701 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
     2761 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
     2821 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
     2881 cggcaactga aagttgaccc gcttcacggc tggcgtgtct gccaggctgg ccaacgttgc
     2941 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
     3001 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
     3061 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
     3121 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
     3181 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
     3241 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
     3301 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
     3361 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
     3421 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
     3481 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
     3541 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
     3601 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
     3661 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
     3721 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
     3781 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
     3841 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
     3901 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
     3961 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg
     4021 gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg
     4081 gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc
     4141 ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc
     4201 aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag
     4261 ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt
     4321 ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
     4381 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
     4441 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
     4501 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
     4561 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
     4621 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
     4681 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
     4741 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
     4801 tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg
     4861 ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg
     4921 attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac
     4981 tgatagttta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
     5041 cccagtcacg acgttgtaaa acgacggcca gtgccagatc tttactctca agataagcgt
     5101 aaacagcatc ttgtcttaga tgcctcctcc catcatttgc aacgacctct gacaccccgc
     5161 acacacttga gcacgcatat attacgataa accatgtatg tgatataaag tatcaactca
     5221 aagcctctac agtgggacta cttatcgtca tcgtctttgt aatccttgta cagctcgtcc
     5281 atgccatgtg taatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc
     5341 tcgttggggt ctttgctcag cttggactga tggctcaggt agtggttgtc gggcagcagc
     5401 acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg
     5461 tcctcaacgt tgtggcggat cttgaagtta gccttgatgc agttcttctg cttgtcggcc
     5521 gtgatataga cgtagtggct gttgaagttg tactccagct tgtgccccag gatgttgccg
     5581 tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac
     5641 ttcacctcgg cgcgggtctt gtaggtaccg tcgtccttga aagagatggt gcgctcctgg
     5701 acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag
     5761 cggctgaagc aggccacgcc gtagccgaag gtggtcacga gggtgggcca gggcacgggc
     5821 agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgttggtggc atcgccctcg
     5881 ccctcgccgc ggacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg
     5941 ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tggatccgga ggaggattcc
     6001 ccatgtttga gcacccgaac ccattccctg taattaaact gtccgtttgc gccaacgggg
     6061 gcccctttaa acatgatatc cacctcctca tccgtaaagc gatcccccat agtcgtcata
     6121 taatccctca acacctccgc attaatgaca cccgacttgt tatcatcaaa acactcaaac
     6181 gcggctagta tctcatgttc aggatccgta ccgctcattt tctcgcccat gagggttaaa
     6241 aacattgtaa agttgatgga accgggtgct tcagagatca tatcgtcaat ggatgattca
     6301 gtcggttgtt ggcctagaga agccaacatg tcttttaaat cctctttatc aataaaccca
     6361 tctgaatcat ggtcaatcat tgaaaacgct tccttgaatt ctgcaatctg tttttggtca
     6421 aacatggcaa agacgtttga gttttggcgg acggcgcgct tctttttctt ttgggctctt
     6481 gcagacattc tagatgctgt gtaaggtgtg cgtgagagag agggtcgtga gtgagtcttg
     6541 aagagaaacg tcaaagcttg aagcgtagca gaatacggaa aacgacttgg gggaatggcg
     6601 aggagcgtcg cccctaggtt ttatgctcca agcggagacg atgaggccga tattacagtg
     6661 gcttagtagc gtgtacaata tttccataaa aggctacatg gtgagttttt cacgtgaccg
     6721 taattagtcc caattaggct ttattgtata ttgctactaa gcatcgttaa atgacctgct
     6781 cgaagcttcg tctataaaag gcgggcgtgt gtgcgcttcc cccgctacgt ctttttcaca
     6841 aacactttgc gacttgacca agacctttca cccgcaccac tctcatccat cacacacaca
     6901 agtcgacatg ggaagcggag ctactaactt cagcctgctg aagcaggctg gagacgtgga
     6961 ggagaaccct ggacctggaa gcggagaggg cagaggaagt ctgctaacat gcggtgacgt
     7021 cgaggagaat cctggaccta ccgagctcgg atctaccatg ggtaaaaagc ctgaactcac
     7081 cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
     7141 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
     7201 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt
     7261 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct
     7321 gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga
     7381 actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct
     7441 tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg
     7501 gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga
     7561 cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga
     7621 ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga
     7681 caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata
     7741 cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg
     7801 ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct
     7861 ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc
     7921 ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac
     7981 acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga
     8041 tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaataatggc gcgcctatat
     8101 aaactcattt acttatgtag gaataaagag tatcatcttt caaagagctc gatatcttaa
     8161 ttaagaattc gtaatcatgt catagctgtt tcctgtgtga aaattcggcg ttaattcagt
     8221 acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac
     8281 aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac
     8341 cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc
     8401 ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt
     8461 tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc
     8521 tgcctgtgat caccgcggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt
     8581 tgaaaacaac tttgaaaagc tgttttctgg tatttaaggt tttagaatgc aaggaacagt
     8641 gaattggagt tcgtcttgtt ataattagct tcttggggta tctttaaata ctgtagaaaa
     8701 gaggaaggaa ataataaatg gctaaaggtg atgctgccaa cttactgatt tagtgtatga
     8761 tggtgttttt gaggtgctcc agtggcttct gtttctatca gctgtccctc ctgttcagct
     8821 actgacgggg tggtgcgtaa cggcaaaagc accgccggac atcagcgcta tctctgctct
     8881 cactgccgta aaacatggca actgcagttc acttacaccg cttctcaacc cggtacgcac
     8941 cagaaaatca ttgatatggc catgaatggc gttggatgcc gggcaacagc ccgcattatg
     9001 ggcgttggcc tcaacacgat tttacgtcac ttaaaaaact caggccgcag tcggtaacct
     9061 cgcgcataca gccgggcagt gacgtcatcg tctgcgcgga aatggacgaa cagtggggct
     9121 atgtcggggc taaatcgcgc cagcgctggc tgttttacgc gtatgacagt ctccggaaga
     9181 cggttgttgc gcacgtattc ggtgaacgca ctatggcgac gctggggcgt cttatgagcc
     9241 tgctgtcacc ctttgacgtg gtgatatgga tgacggatgg ctggccgctg tatgaatccc
     9301 gcctgaaggg aaagctgcac gtaatcagca agcgatatac gcagcgaatt gagcggcata
     9361 acctgaatct gaggcagcac ctggcacggc tgggacggaa gtcgctgtcg ttctcaaaat
     9421 cggtggagct gcatgacaaa gtcatcgggc attatctgaa cataaaacac tatcaataag
     9481 ttggagtcat taccatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa
     9541 aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg
     9601 gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg
     9661 atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg
     9721 tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg
     9781 tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg
     9841 cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct
     9901 tagacagccg cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt
     9961 gcgaaaactg ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa
    10021 agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca
    10081 tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg
    10141 acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac
    10201 agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat
    10261 attatatttt actggatgaa ttgttttagt acctagaatg catgaccaaa atcccttaac
    10321 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttc
//
