LOCUS       pLH2259-pUBC-SV4        9342 bp    DNA     circular SYN 19-OCT-2022
DEFINITION  Hernandez_Y7Q_4_pUBC-SV40NLS-POPZ3H-QTE-4     coverage: 77.4x.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 4, 2025 from SnapGene 8.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    246..834
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(717..728)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    1494..1674
                     /label=truncated 5' long terminal repeat (LTR)from HIV-1
     primer_bind     complement(1802..1818)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1826..1842
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1850..1856,1857..1874,1875..1880))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1895..1916
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    complement(2186..2868)
                     /label=ColE1 origin
     misc_feature    complement(2966..3625)
                     /label=AmpR
     misc_feature    4278..4911
                     /label=3' long terminal repeat (LTR) from HIV-1
     misc_feature    4731..4911
                     /label=truncated 5' long terminal repeat (LTR)from HIV-1
     misc_feature    5580..5813
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     promoter        6466..7677
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     misc_feature    7699..7719
                     /label=SV40NLS
     misc_feature    7726..7954
                     /label=PopZ
     misc_feature    7960..8805
                     /label=QtEncapsulin_Mammalian_CodonOptimized
     misc_feature    8809..8841
                     /label=GS linker
     misc_feature    join(8854..9342,1..228)
                     /label=TSapphire (mammalian codon-optimized)
ORIGIN
        1 aacttcaaga ttcggcacaa tattgaagac ggtagtgtac agctggctga ccactatcag
       61 cagaatacgc caattggtga tgggcccgtc ttgttgccag ataatcacta cctctcaatc
      121 cagagtgccc ttagtaaaga tccaaacgaa aagcgggacc acatggtttt gttggagttt
      181 gtcacagctg ctgggattac ccacgggata gacgagttgt ataaatgact cgacgcgtct
      241 ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
      301 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
      361 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
      421 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
      481 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
      541 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
      601 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
      661 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
      721 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
      781 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
      841 ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
      901 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
      961 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     1021 tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct
     1081 tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg
     1141 gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc
     1201 agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct
     1261 gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc
     1321 atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc
     1381 ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg
     1441 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc
     1501 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     1561 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     1621 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     1681 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     1741 agaggccttg acattgctag cgtttaccgt cgacctctag ctagagcttg gcgtaatcat
     1801 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     1861 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     1921 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     1981 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     2041 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     2101 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     2161 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     2221 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2281 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     2341 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     2401 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     2461 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     2521 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     2581 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     2641 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     2701 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2761 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     2821 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     2881 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     2941 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     3001 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     3061 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     3121 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     3181 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     3241 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     3301 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     3361 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     3421 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     3481 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     3541 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     3601 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     3661 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     3721 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     3781 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     3841 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     3901 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     3961 acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     4021 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     4081 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
     4141 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
     4201 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
     4261 actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga
     4321 tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca caccagggcc
     4381 aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga
     4441 taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca
     4501 tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt
     4561 tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg
     4621 ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga
     4681 gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct
     4741 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     4801 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     4861 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     4921 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     4981 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     5041 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     5101 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     5161 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     5221 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     5281 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     5341 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     5401 taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg aggagatatg
     5461 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     5521 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     5581 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     5641 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     5701 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     5761 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     5821 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     5881 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     5941 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     6001 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     6061 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     6121 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     6181 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     6241 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     6301 cgcctttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     6361 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     6421 tcgggtttat tacagggaca gcagagatcc agtttattta attaaggcct ccgcgccggg
     6481 ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag cgctgccacg tcagacgaag
     6541 ggcgcagcga gcgtcctgat ccttccgccc ggacgctcag gacagcggcc cgctgctcat
     6601 aagactcggc cttagaaccc cagtatcagc agaaggacat tttaggacgg gacttgggtg
     6661 actctagggc actggttttc tttccagaga gcggaacagg cgaggaaaag tagtcccttc
     6721 tcggcgattc tgcggaggga tctccgtggg gcggtgaacg ccgatgatta tataaggacg
     6781 cgccgggtgt ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg
     6841 tggatcgctg tgatcgtcac ttggtgagta gcgggctgct gggctggccg gggctttcgt
     6901 ggccgccggg ccgctcggtg ggacggaagc gtgtggagag accgccaagg gctgtagtct
     6961 gggtccgcga gcaaggttgc cctgaactgg gggttggggg gagcgcagca aaatggcggc
     7021 tgttcccgag tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag
     7081 gtggggggca tggtgggcgg caagaaccca aggtcttgag gccttcgcta atgcgggaaa
     7141 gctcttattc gggtgagatg ggctggggca ccatctgggg accctgacgt gaagtttgtc
     7201 actgactgga gaactcggtt tgtcgtctgt tgcgggggcg gcagttatgg cggtgccgtt
     7261 gggcagtgca cccgtacctt tgggagcgcg cgccctcgtc gtgtcgtgac gtcacccgtt
     7321 ctgttggctt ataatgcagg gtggggccac ctgccggtag gtgtgcggta ggcttttctc
     7381 cgtcgcagga cgcagggttc gggcctaggg taggctctcc tgaatcgaca ggcgccggac
     7441 ctctggtgag gggagggata agtgaggcgt cagtttcttt ggtcggtttt atgtacctat
     7501 cttcttaagt agctgaagct ccggttttga actatgcgct cggggttggc gagtgtgttt
     7561 tgtgaagttt tttaggcacc ttttgaaatg taatcatttg ggtcaatatg taattttcag
     7621 tgttagacta gtaaattgtc cgctaaattc tggccgtttt tggctttttt gttagacgaa
     7681 ttcgccacca tgtctagacc aaaaaagaag agaaaggtct ctagagaggt cgcagagcag
     7741 ctggttggcg tctctgccgc ctctgcggct gcaagcgcct ttggctcact gtcaagcgcc
     7801 ttattaatgc ccaaagacgg cagaacactg gaggacgtag tgagagagct gctgcggcca
     7861 ctgttgaagg agtggctaga ccagaacctc cctcgcattg tagagaccaa ggttgaagag
     7921 gaagtgcagc ggatcagtag aggtcggggc gcatctagaa tgaataagtc tcagctgtac
     7981 ccagacagtc ccctcaccga ccaagatttt aatcagttgg atcagacagt tatcgaagca
     8041 gcacggcgcc aattggtcgg ccgacgattt atcgaactgt acggacccct cggacgcggg
     8101 atgcaaagcg tgtttaatga cattttcatg gaaagtcacg aggctaagat ggattttcaa
     8161 gggtccttcg ataccgaagt ggaatcttca agacgcgtca attacacaat ccctatgctg
     8221 tacaaggatt ttgttctgta ttggcgggac cttgagcaat ctaaagcgct ggacatcccc
     8281 atagatttca gtgtagctgc taatgccgct agggatgtgg cctttcttga ggaccaaatg
     8341 atctttcacg gctcaaagga gttcgacatt cctgggttaa tgaatgtcaa ggggcggctc
     8401 acacacctca tcggaaactg gtacgaatcc ggcaatgctt tccaagacat cgtcgaagca
     8461 aggaacaagt tgttagagat gaatcataac gggccgtacg ccctggtttt gtcaccagaa
     8521 ctttatagcc tgctccaccg ggtacacaag gatactaacg ttctcgaaat tgagcatgtc
     8581 agggaactga taaccgccgg tgtattccaa agccccgtac tgaagggaaa gtccggagtc
     8641 attgtgaata cgggacggaa taacctcgac ctcgcaataa gcgaggactt cgaaaccgct
     8701 tatttgggag aagagggcat gaatcaccca ttcagggtct atgaaaccgt ggtcttgaga
     8761 ataaagaggc cagccgcgat ctgcaccctc attgaccctg aggagtggtc agggtcaggt
     8821 tctggctcag gtagcgggag tgggcggccg ctgatgtcta agggggagga gcttttcaca
     8881 ggtgtagtac ctattcttgt tgaattggat ggcgatgtca atggacacaa attctccgtc
     8941 tcaggggagg gagagggaga cgctacctat ggaaagttga ctctcaaatt catctgcaca
     9001 acgggtaagt tgccggtccc ttggccgaca ctcgttacta cgttcagcta tggagtacag
     9061 tgtttcgcgc gctaccccga ccatatgaaa caacacgact ttttcaaatc cgcaatgccc
     9121 gagggatacg ttcaggaacg gactattttc tttaaagacg acggtaatta caaaacacgc
     9181 gccgaagtta agttcgaagg agatactttg gttaaccgaa tagagctgaa aggcattgat
     9241 ttcaaagagg acgggaacat cctgggtcat aaacttgagt ataattttaa ttcccacaac
     9301 gtgtatataa tggcagataa acagaagaat ggcatcaagg tt
//
