LOCUS       pGLU_387                6045 bp ds-DNA     circular     11-APR-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     prokaryote p... complement(352..803)
                     /label="PermC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(773..832)
                     /label="oLO.225"
                     /note="sequence: CCCTACTCGACGCGTCCTACGCGTGACCCGATCCTTTAACTCTGGCAACCCTCAAAATTG"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    804..833
                     /label="F primer binding"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          833..880
                     /label="oLO.230"
                     /note="sequence: TAATGTCCGACGGGGAGTATATACTGCATGAAGAGCGAAAGTGAAACG"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     Barcode         838..857
                     /label="BC.0005"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     prokaryote p... 869..998
                     /label="BBa_J72163 GlpT Promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     RBS             999..1034
                     /label="sfGFP Ribosome Binding Site"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             1035..1751
                     /label="sfGFP"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     prokaryote t... 1752..1841
                     /label="ECK120029600"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             1850..2896
                     /label="Himar.007"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     prokaryote t... 2901..3081
                     /label="TAmarelleT9"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     miscellaneous   3082..3327
                     /label="oriT"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     miscellaneous   complement(3336..3375)
                     /label="UNS1"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     prokaryote t... complement(3376..3405)
                     /label="L2U5H11"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(3458..3474)
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     promoter        3588..3692
                     /label="Pamp"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             3693..4553
                     /label="AmpR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     ORI             complement(4810..5198)
                     /label="R6K ORI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     5388..5404
                     /label="M13 rev"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     prokaryote p... complement(5461..5492)
                     /label="L2U8H11"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     miscellaneous   complement(5493..5532)
                     /label="UNSX"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     miscellaneous   complement(5541..5570)
                     /label="Himar_RE"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     prokaryote t... complement(5571..5656)
                     /label="TermC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             complement(5661..350)
                     /label="ermC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 TCAAAAACAA TTTTGCGTAT TATATCCGTA CTTATGTTAT AAGGTATATT ACCATATATT
       61 TTATAGGATT GGTTTTTAGG AAATTTAAAC TGCAATATAT CCTTGTTTAA AACTTGGAAA
      121 TTATCGTGAT CAACAAGTTT ATTTTCTGTA GTTTTGCATA ATTTATGGTC TATTTCAATG
      181 GCAGTTACGA AATTACACCT CTTTACTAAT TCAAGGGTAA AATGGCCTTT TCCTGAGCCG
      241 ATTTCAAAGA TATTATCATG TTCATTTAAT CTTATGTTTG TCATAATCTT GTCGATATTA
      301 TGCTTAGAAG TAATAAAGTT TTGACTGTGT TTTATATTTT TCTCGTTCAT TTATAACCCT
      361 CTTTAATTTG GTTATATGAA TTTTGCTTAT TAACGATTCA TTATAACCAC TTATTTTTTG
      421 TTTGGTTGAT AATGAACTGT GCTGATTACA AAAATACTAA AAATGCCCAT ATTTTTTCCT
      481 CCTTATAAAA TTAGTATAAT TATAGCACGA CCTCTGATAA ATATGAACAT GATGAGTGAT
      541 CGTTAAATTT ATACTGCAAT CGGATGCGAT TATTGAATAA AAGATATGAG AGATTTATCT
      601 AATTTCTTTT TTCTTGTAAA AAAAGAAAGT TCTTAAAGGT TTTATAGTTT TGGTCGTAGA
      661 GCACACGGTT TAACGACTTA ATTACGAAGT AAATAAGTCT AGTGTGTTAG ACTTTATGAA
      721 ATCTATATAC GTTTATATAT ATTTATTATC CGGAGGTGTA GCATGTCTCA TTCAATTTTG
      781 AGGGTTGCCA GAGTTAAAGG ATCGGGTCAC GCGTAGGACG CGTCGAGTAG GGTAATGTCC
      841 GACGGGGAGT ATATACTGCA TGAAGAGCGA AAGTGAAACG TGATTTCATG CGTCATTTTG
      901 AACATTTTGT AAATCTTATT TAATAATGTG TGCGGCAATT CACATTTAAT TTATGAATGT
      961 TTTCTTAACA TCGCGGCAAC TCAAGAAACG GCAGGTTCGG ATCTTAGCTA CTAGAGAAAG
     1021 AGGAGAAATA CTAGATGCGT AAAGGCGAAG AACTGTTCAC TGGTGTCGTC CCTATTCTGG
     1081 TGGAACTGGA TGGTGATGTC AACGGTCATA AGTTTTCCGT GCGTGGCGAG GGTGAAGGTG
     1141 ACGCAACTAA TGGTAAACTG ACGCTGAAGT TCATCTGTAC TACTGGTAAA CTGCCGGTTC
     1201 CTTGGCCGAC TCTGGTAACG ACGCTGACTT ATGGTGTTCA GTGCTTTGCT CGTTATCCGG
     1261 ACCATATGAA GCAGCATGAC TTCTTCAAGT CCGCCATGCC GGAAGGCTAT GTGCAGGAAC
     1321 GCACGATTTC CTTTAAGGAT GACGGCACGT ACAAAACGCG TGCGGAAGTG AAATTTGAAG
     1381 GCGATACCCT GGTAAACCGC ATTGAGCTGA AAGGCATTGA CTTTAAAGAG GACGGCAATA
     1441 TCCTGGGCCA TAAGCTGGAA TACAATTTTA ACAGCCACAA TGTTTACATC ACCGCCGATA
     1501 AACAAAAAAA TGGCATTAAA GCGAATTTTA AAATTCGCCA CAACGTGGAG GATGGCAGCG
     1561 TGCAGCTGGC TGATCACTAC CAGCAAAACA CTCCAATCGG TGATGGTCCT GTTCTGCTGC
     1621 CAGACAATCA CTATCTGAGC ACGCAAAGCG TTCTGTCTAA AGATCCGAAC GAGAAACGCG
     1681 ATCATATGGT TCTGCTGGAG TTCGTAACCG CAGCGGGCAT CACGCATGGT ATGGATGAAC
     1741 TGTACAAATG ATTCAGCCAA AAAACTTAAG ACCGCCGGTC TTGTCCACTA CCTTGCAGTA
     1801 ATGCGGTGGA CAGGATCGGC GGTTTTCTTT TCTCTTCTCA AGCTCTTCAA TGGAAAAAAA
     1861 AGAATTTCGT GTTTTAATCA AATATTGTTT TTTAAAAGGA AAAAATACAG TTGAAGCAAA
     1921 AACATGGTTA GATAATGAAT TTCCAGATTC AGCACCAGGA AAATCAACAA TTATTGATTG
     1981 GTATGCAAAA TTCAAACGTG GTGAAATGTC AACGGAAGAT GGTGAACGTA GTGGACGTCC
     2041 AAAAGAAGTT GTTACAGATG AAAATATTAA AAAAATTCAT AAAATGATTC TTAATGACCG
     2101 TAAAATGAAA TTAATTGAAA TTGCAGAAGC ATTAAAAATT TCAAAAGAAC GTGTTGGTCA
     2161 TATTATTCAT CAATATCTTG ATATGCGTAA GTTATGTGCG AAATGGGTTC CACGTGAATT
     2221 AACATTTGAT CAAAAACAAC GTCGTGTTGA TGATTCAAAA CGTTGTTTAC AATTATTAAC
     2281 ACGTAATACA CCAGAATTTT TTCGTCGTTA TGTTACAATG GATGAAACAT GGTTACATCA
     2341 TTATACACCT GAATCAAATC GTCAATCGGC TGAATGGACA GCGACAGGTG AACCATCGCC
     2401 AAAACGTGGA AAAACACAAA AATCAGCTGG CAAAGTAATG GCATCAGTTT TTTGGGATGC
     2461 GCATGGAATT ATTTTTATTG ATTATCTTGA AAAAGGAAAA ACGATTAATA GTGATTATTA
     2521 TATGGCGTTA TTAGAACGTT TAAAAGTCGA AATTGCGGCA AAACGTCCAC ATATGAAAAA
     2581 AAAAAAAGTT TTATTTCATC AAGATAATGC ACCATGTCAC AAATCATTAA GAACGATGGC
     2641 AAAAATTCAT GAATTAGGCT TTGAATTATT ACCTCATCCA CCATATTCAC CAGATCTTGC
     2701 ACCATCAGAT TTTTTTTTAT TTTCAGATCT TAAAAGAATG CTAGCAGGTA AAAAATTTGG
     2761 CTGTAATGAA GAAGTTATTG CAGAAACAGA AGCATATTTT GAAGCAAAAC CAAAAGAATA
     2821 TTATCAAAAT GGTATTAAAA AATTAGAAGG TCGTTATAAT CGTTGTATTG CTTTAGAAGG
     2881 TAATTATGTT GAATAAGCTT GGAATCTCCT TCTCGCCTCT TTCGCTGAAC ACGAAAGCGA
     2941 ATCCGTTCAG CACACACTTT ACGATATGGC GCAAAAAGTC CTCGGCTGCG TGCCGGAAGT
     3001 GAAAGACATC CACCTCACCA TGCCCAACAA GCATTGCCTG CTCGTGGACC TGTCCCGCTT
     3061 CGGTCAGGAC AATCCCAACG ACTTTTCCGC TGCATAACCC TGCTTCGGGG TCATTATAGC
     3121 GATTTTTTCG GTATATCCAT CCTTTTTCGC ACGATATACA GGATTTTGCC AAAGGGTTCG
     3181 TGTAGACTTT CCTTGGTGTA TCCAACGGCG TCAGCCGGGC AGGATAGGTG AAGTAGGCCC
     3241 ACCCGCGAGC GGGTGTTCCT TCTTCACTGT CCCTTATTCG CACCTGGCGG TGCTCAACGG
     3301 GAATCCTGCT CTGCGAGGCT GGCCGTACTC CTTCGGAGAC GAGACGAGAC AGCCTGAGAA
     3361 TGGATGCGAG TAATGATACA ACAATAGCGT GCTGTTCGCA CGCTAAGGTC AGGCGGAATG
     3421 GCACTTCCTT ATTCCAGATG CGTGCGGATT ATGCATGACT GGCCGTCGTT TTACAACGTC
     3481 GTGACTGGGA AAACCCTGGC GCTTGTGGTG GATCACAGTT ATTGGAGACT TCCTTGGCCG
     3541 TACGATGGGT TTTGTTTCAG GTATCTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA
     3601 TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT
     3661 AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC
     3721 TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA
     3781 AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA
     3841 ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT
     3901 TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG
     3961 GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC
     4021 ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA
     4081 ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT
     4141 TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG
     4201 CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA
     4261 AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG
     4321 AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG
     4381 CTGATAAATC TGGAGCCGGT GAGCGTGGCT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG
     4441 ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG
     4501 AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG
     4561 ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA
     4621 TCTAGGTGAA GATCCTTGAA TTGCCATTAT CAACAAGTTT GTACAAAAAA GCAGGCTCCG
     4681 AATTGGTATC ACGAGGACGA TTTGAAGCCC CCTACCCATT TACGCTGACT TGACGGGACG
     4741 GCGGCTTTGT TGAATAGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCGGCCGGC
     4801 CGGCGCGCCG ATCTGAAGAT CAGCAGTTCA ACCTGTTGAT AGTACGTACT AAGCTCTCAT
     4861 GTTTCACGTA CTAAGCTCTC ATGTTTAACG TACTAAGCTC TCATGTTTAA CGAACTAAAC
     4921 CCTCATGGCT AACGTACTAA GCTCTCATGG CTAACGTACT AAGCTCTCAT GTTTCACGTA
     4981 CTAAGCTCTC ATGTTTGAAC AATAAAATTA ATATAAATCA GCAACTTAAA TAGCCTCTAA
     5041 GGTTTTAAGT TTTATAAGAA AAAAAAGAAT ATATAAGGCT TTTAAAGCCT TTAAGGTTTA
     5101 ACGGTTGTGG ACAACAAGCC AGGGATGTAA CGCACTGAGA AGCCCTTAGA GCCTCTCAAA
     5161 GCAATTTTGA GTGACACAGG AACACTTAAC GGCTGACATG GGGCGCGCCC AGCTGTAACG
     5221 CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT
     5281 TTCCAGCGAC ATATGCCATA CGCCGAACTG AACTCCACCT AATGTCTTGG AGATTGTCGG
     5341 ATAGCGCGGA ATAGGATTGT GCCCTCTGAG CGGATAACAA TTTCACACAG GAAACAGCTA
     5401 TGACCATGAC ACATGCATCC ACCATCGCAG ACTTATCATC ACTGAGCCTC CACCTAGCCT
     5461 ATACAACAAT AGCGTGCGTG GTTAGCACGC TAGGTGGAAG GGCTCGGAGT TGTGGTAATC
     5521 TATGTATCCT GGGGTTAGCG TAACAGGTTG GATGATAAGT CCCCGGTCTA GCCGACTGCG
     5581 CAAAAGACAT AATCGATTCA CAAAAAATAG GCACACGAAA AACAAGTTAA GGGATGCAGT
     5641 TTATGCATCC CTTAACAAGC TTACTTATTA AATAATTTAT AGCTATTGAA AAGAGATAAG
     5701 AATTGTTCAA AGCTAATATT GTTTAAATCG TCAATTCCTG CATGTTTTAA GGAATTGTTA
     5761 AATTGATTTT TTGTAAATAT TTTCTTGTAT TCTTTGTTAA CCCATTTCAT AACGAAATAA
     5821 TTATACTTTT GTTTATCTTT GTGTGATATT CTTGATTTTT TTCTACTTAA TCTGATAAGT
     5881 GAGCTATTCA CTTTAGGTTT AGGATGAAAA TATTCTCTTG GAACCATACT TAATATAGAA
     5941 ATATCAACTT CTGCCATTAA AAGTAATGCC AATGAGCGTT TTGTATTTAA TAATCTTTTA
     6001 GCAAACCCGT ATTCCACGAT TAAATAAATC TCATTAGCTA TACTA
//