LOCUS       pGLU_389                6418 bp ds-DNA     circular     28-APR-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     prokaryote t... 388..477
                     /label="ECK120029600"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             486..1916
                     /label="Tn5.002"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     prokaryote t... 1921..2101
                     /label="TAmarelleT9"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     miscellaneous   2102..2347
                     /label="oriT"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     miscellaneous   complement(2356..2395)
                     /label="UNS1"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     prokaryote t... complement(2396..2425)
                     /label="L2U5H11"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(2478..2494)
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(2487..2509)
                     /label="M13/pUC Forward"
                     /note="sequence: CCCAGTCACGACGTTGTAAAACG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     promoter        2608..2712
                     /label="Pamp"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             2713..3573
                     /label="AmpR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     ORI             complement(3830..4218)
                     /label="R6K ORI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          4389..4411
                     /label="M13/pUC Reverse"
                     /note="sequence: AGCGGATAACAATTTCACACAGG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     4408..4424
                     /label="M13 rev"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     prokaryote p... complement(4481..4512)
                     /label="L2U8H11"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     miscellaneous   complement(4513..4552)
                     /label="UNSX"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     miscellaneous   complement(4561..4579)
                     /label="Tn5_RE"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     prokaryote t... complement(4580..4665)
                     /label="TermC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             complement(4670..5404)
                     /label="ermC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          5405..5449
                     /label="oLO.223"
                     /note="sequence: ATGCTCTTCACATTATAACCCTCTTTAATTTGGTTATATGAATTTTGCTTATTAACG"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     prokaryote p... complement(5406..5857)
                     /label="PermC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(5827..5886)
                     /label="oLO.225"
                     /note="sequence: CCCTACTCGACGCGTCCTACGCGTGACCCGATCCTTTAACTCTGGCAACCCTCAAAATTG"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    5858..5887
                     /label="F primer binding"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          5887..5934
                     /label="oLO.232"
                     /note="sequence: TAATGGTACATGAAACGATGGACGGGCATGAAGAGCGAAAGTGAAACG"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     Barcode         5892..5911
                     /label="BC.0007"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     prokaryote p... 5923..6052
                     /label="BBa_J72163 GlpT Promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     RBS             6053..6088
                     /label="sfGFP Ribosome Binding Site"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             6089..387
                     /label="sfGFP"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 GAAGTGAAAT TTGAAGGCGA TACCCTGGTA AACCGCATTG AGCTGAAAGG CATTGACTTT
       61 AAAGAGGACG GCAATATCCT GGGCCATAAG CTGGAATACA ATTTTAACAG CCACAATGTT
      121 TACATCACCG CCGATAAACA AAAAAATGGC ATTAAAGCGA ATTTTAAAAT TCGCCACAAC
      181 GTGGAGGATG GCAGCGTGCA GCTGGCTGAT CACTACCAGC AAAACACTCC AATCGGTGAT
      241 GGTCCTGTTC TGCTGCCAGA CAATCACTAT CTGAGCACGC AAAGCGTTCT GTCTAAAGAT
      301 CCGAACGAGA AACGCGATCA TATGGTTCTG CTGGAGTTCG TAACCGCAGC GGGCATCACG
      361 CATGGTATGG ATGAACTGTA CAAATGATTC AGCCAAAAAA CTTAAGACCG CCGGTCTTGT
      421 CCACTACCTT GCAGTAATGC GGTGGACAGG ATCGGCGGTT TTCTTTTCTC TTCTCAAGCT
      481 CTTCAATGAT TACATCAGCA TTACATCGTG CAGCAGATTG GGCAAAATCA GTTTTTTCAT
      541 CAGCAGCATT AGGTGATCCA CGTCGTACAG CACGTTTAGT TAATGTTGCA GCACAATTAG
      601 CAAAATATTC AGGTAAATCA ATTACAATTT CATCAGAAGG TTCAAAAGCA ATGCAAGAAG
      661 GTGCATATCG TTTTATTCGT AATCCAAATG TTTCAGCAGA AGCAATTCGT AAAGCAGGTG
      721 CAATGCAAAC AGTTAAATTA GCACAAGAAT TTCCAGAATT ATTAGCAATT GAAGATACAA
      781 CATCATTATC ATATCGTCAT CAAGTTGCAG AAGAATTAGG TAAATTAGGT TCAATTCAAG
      841 ATAAATCACG TGGTTGGTGG GTTCATTCAG TTTTATTATT AGAAGCAACA ACATTTCGTA
      901 CAGTTGGTTT ATTACATCAA GAATGGTGGA TGCGTCCAGA TGATCCAGCA GATGCAGATG
      961 AAAAAGAATC AGGTAAATGG TTAGCAGCAG CAGCAACATC ACGTTTACGT ATGGGTTCAA
     1021 TGATGTCAAA TGTTATTGCA GTTTGTGATC GTGAAGCAGA TATTCATGCA TATTTACAAG
     1081 ATAAATTAGC ACATAATGAA CGTTTTGTTG TTCGTTCAAA ACATCCACGT AAAGATGTTG
     1141 AATCAGGTTT ATATTTATAT GATCATTTAA AAAATCAACC AGAATTAGGT GGTTATCAAA
     1201 TTTCAATTCC ACAAAAAGGT GTTGTTGATA AACGTGGTAA ACGTAAAAAT CGTCCAGCAC
     1261 GTAAAGCATC ATTATCATTA CGTTCAGGTC GTATTACATT AAAACAAGGT AATATTACAT
     1321 TAAATGCAGT TTTAGCAGAA GAAATTAATC CACCAAAAGG TGAAACACCA TTAAAATGGT
     1381 TATTATTAAC ATCAGAACCA GTTGAATCAT TAGCACAAGC ATTACGTGTT ATTGATATTT
     1441 ATACACATCG TTGGCGTATT GAAGAATTTC ATAAAGCATG GAAAACAGGT GCAGGTGCAG
     1501 AACGTCAACG TATGGAAGAA CCAGATAATT TAGAACGTAT GGTTTCAATT TTATCATTTG
     1561 TTGCAGTTCG TTTATTACAA TTACGTGAAT CATTTACACC ACCACAAGCA TTACGTGCAC
     1621 AAGGTTTATT AAAAGAAGCA GAACATGTTG AATCACAATC AGCAGAAACA GTTTTAACAC
     1681 CAGATGAATG TCAATTATTA GGTTATTTAG ATAAAGGTAA ACGTAAACGT AAAGAAAAAG
     1741 CAGGTTCATT ACAATGGGCA TATATGGCAA TTGCACGTTT AGGTGGTTTT ATGGATTCAA
     1801 AACGTACAGG TATTGCATCA TGGGGTGCAT TATGGGAAGG TTGGGAAGCA TTACAATCAA
     1861 AATTAGATGG TTTTTTAGCA GCAAAAGATT TAATGGCACA AGGTATTAAA ATTTAAGCTT
     1921 GGAATCTCCT TCTCGCCTCT TTCGCTGAAC ACGAAAGCGA ATCCGTTCAG CACACACTTT
     1981 ACGATATGGC GCAAAAAGTC CTCGGCTGCG TGCCGGAAGT GAAAGACATC CACCTCACCA
     2041 TGCCCAACAA GCATTGCCTG CTCGTGGACC TGTCCCGCTT CGGTCAGGAC AATCCCAACG
     2101 ACTTTTCCGC TGCATAACCC TGCTTCGGGG TCATTATAGC GATTTTTTCG GTATATCCAT
     2161 CCTTTTTCGC ACGATATACA GGATTTTGCC AAAGGGTTCG TGTAGACTTT CCTTGGTGTA
     2221 TCCAACGGCG TCAGCCGGGC AGGATAGGTG AAGTAGGCCC ACCCGCGAGC GGGTGTTCCT
     2281 TCTTCACTGT CCCTTATTCG CACCTGGCGG TGCTCAACGG GAATCCTGCT CTGCGAGGCT
     2341 GGCCGTACTC CTTCGGAGAC GAGACGAGAC AGCCTGAGAA TGGATGCGAG TAATGATACA
     2401 ACAATAGCGT GCTGTTCGCA CGCTAAGGTC AGGCGGAATG GCACTTCCTT ATTCCAGATG
     2461 CGTGCGGATT ATGCATGACT GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC
     2521 GCTTGTGGTG GATCACAGTT ATTGGAGACT TCCTTGGCCG TACGATGGGT TTTGTTTCAG
     2581 GTATCTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA
     2641 CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA
     2701 AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA
     2761 TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT
     2821 CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG
     2881 AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC
     2941 GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT
     3001 CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA
     3061 GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT
     3121 CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT
     3181 GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT
     3241 GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA
     3301 CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA
     3361 CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT
     3421 GAGCGTGGCT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC
     3481 GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT
     3541 GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA
     3601 CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTGAA
     3661 TTGCCATTAT CAACAAGTTT GTACAAAAAA GCAGGCTCCG AATTGGTATC ACGAGGACGA
     3721 TTTGAAGCCC CCTACCCATT TACGCTGACT TGACGGGACG GCGGCTTTGT TGAATAGAGC
     3781 GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCGGCCGGC CGGCGCGCCG ATCTGAAGAT
     3841 CAGCAGTTCA ACCTGTTGAT AGTACGTACT AAGCTCTCAT GTTTCACGTA CTAAGCTCTC
     3901 ATGTTTAACG TACTAAGCTC TCATGTTTAA CGAACTAAAC CCTCATGGCT AACGTACTAA
     3961 GCTCTCATGG CTAACGTACT AAGCTCTCAT GTTTCACGTA CTAAGCTCTC ATGTTTGAAC
     4021 AATAAAATTA ATATAAATCA GCAACTTAAA TAGCCTCTAA GGTTTTAAGT TTTATAAGAA
     4081 AAAAAAGAAT ATATAAGGCT TTTAAAGCCT TTAAGGTTTA ACGGTTGTGG ACAACAAGCC
     4141 AGGGATGTAA CGCACTGAGA AGCCCTTAGA GCCTCTCAAA GCAATTTTGA GTGACACAGG
     4201 AACACTTAAC GGCTGACATG GGGCGCGCCC AGCTGTAACG CCAGCAACGC GGCCTTTTTA
     4261 CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCAGCGAC ATATGCCATA
     4321 CGCCGAACTG AACTCCACCT AATGTCTTGG AGATTGTCGG ATAGCGCGGA ATAGGATTGT
     4381 GCCCTCTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAC ACATGCATCC
     4441 ACCATCGCAG ACTTATCATC ACTGAGCCTC CACCTAGCCT ATACAACAAT AGCGTGCGTG
     4501 GTTAGCACGC TAGGTGGAAG GGCTCGGAGT TGTGGTAATC TATGTATCCT GGGGTTAGCG
     4561 CTGTCTCTTA TACACATCTG CCGACTGCGC AAAAGACATA ATCGATTCAC AAAAAATAGG
     4621 CACACGAAAA ACAAGTTAAG GGATGCAGTT TATGCATCCC TTAACAAGCT TACTTATTAA
     4681 ATAATTTATA GCTATTGAAA AGAGATAAGA ATTGTTCAAA GCTAATATTG TTTAAATCGT
     4741 CAATTCCTGC ATGTTTTAAG GAATTGTTAA ATTGATTTTT TGTAAATATT TTCTTGTATT
     4801 CTTTGTTAAC CCATTTCATA ACGAAATAAT TATACTTTTG TTTATCTTTG TGTGATATTC
     4861 TTGATTTTTT TCTACTTAAT CTGATAAGTG AGCTATTCAC TTTAGGTTTA GGATGAAAAT
     4921 ATTCTCTTGG AACCATACTT AATATAGAAA TATCAACTTC TGCCATTAAA AGTAATGCCA
     4981 ATGAGCGTTT TGTATTTAAT AATCTTTTAG CAAACCCGTA TTCCACGATT AAATAAATCT
     5041 CATTAGCTAT ACTATCAAAA ACAATTTTGC GTATTATATC CGTACTTATG TTATAAGGTA
     5101 TATTACCATA TATTTTATAG GATTGGTTTT TAGGAAATTT AAACTGCAAT ATATCCTTGT
     5161 TTAAAACTTG GAAATTATCG TGATCAACAA GTTTATTTTC TGTAGTTTTG CATAATTTAT
     5221 GGTCTATTTC AATGGCAGTT ACGAAATTAC ACCTCTTTAC TAATTCAAGG GTAAAATGGC
     5281 CTTTTCCTGA GCCGATTTCA AAGATATTAT CATGTTCATT TAATCTTATG TTTGTCATAA
     5341 TCTTGTCGAT ATTATGCTTA GAAGTAATAA AGTTTTGACT GTGTTTTATA TTTTTCTCGT
     5401 TCATTTATAA CCCTCTTTAA TTTGGTTATA TGAATTTTGC TTATTAACGA TTCATTATAA
     5461 CCACTTATTT TTTGTTTGGT TGATAATGAA CTGTGCTGAT TACAAAAATA CTAAAAATGC
     5521 CCATATTTTT TCCTCCTTAT AAAATTAGTA TAATTATAGC ACGACCTCTG ATAAATATGA
     5581 ACATGATGAG TGATCGTTAA ATTTATACTG CAATCGGATG CGATTATTGA ATAAAAGATA
     5641 TGAGAGATTT ATCTAATTTC TTTTTTCTTG TAAAAAAAGA AAGTTCTTAA AGGTTTTATA
     5701 GTTTTGGTCG TAGAGCACAC GGTTTAACGA CTTAATTACG AAGTAAATAA GTCTAGTGTG
     5761 TTAGACTTTA TGAAATCTAT ATACGTTTAT ATATATTTAT TATCCGGAGG TGTAGCATGT
     5821 CTCATTCAAT TTTGAGGGTT GCCAGAGTTA AAGGATCGGG TCACGCGTAG GACGCGTCGA
     5881 GTAGGGTAAT GGTACATGAA ACGATGGACG GGCATGAAGA GCGAAAGTGA AACGTGATTT
     5941 CATGCGTCAT TTTGAACATT TTGTAAATCT TATTTAATAA TGTGTGCGGC AATTCACATT
     6001 TAATTTATGA ATGTTTTCTT AACATCGCGG CAACTCAAGA AACGGCAGGT TCGGATCTTA
     6061 GCTACTAGAG AAAGAGGAGA AATACTAGAT GCGTAAAGGC GAAGAACTGT TCACTGGTGT
     6121 CGTCCCTATT CTGGTGGAAC TGGATGGTGA TGTCAACGGT CATAAGTTTT CCGTGCGTGG
     6181 CGAGGGTGAA GGTGACGCAA CTAATGGTAA ACTGACGCTG AAGTTCATCT GTACTACTGG
     6241 TAAACTGCCG GTTCCTTGGC CGACTCTGGT AACGACGCTG ACTTATGGTG TTCAGTGCTT
     6301 TGCTCGTTAT CCGGACCATA TGAAGCAGCA TGACTTCTTC AAGTCCGCCA TGCCGGAAGG
     6361 CTATGTGCAG GAACGCACGA TTTCCTTTAA GGATGACGGC ACGTACAAAA CGCGTGCG
//