LOCUS       pLJM1-CRS-Halo-R       10894 bp DNA     circular SYN 11-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10894)
  AUTHORS   Azra Lari
  TITLE     Direct Submission
  JOURNAL   Exported Dec 11, 2025 from SnapGene 6.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          789..1673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        131..334
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      390..395
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             396..758
                     /codon_start=1
                     /label=GDOWN1-CRS
                     /translation="MAKTNVLPFRQNDSSSHCQKSGSPISSEERRRRDKQHLDDITAAR
                     LLPLHHMPTQLLSIEESLALQKQQKQNYEEMQAKLAAQKLAERLNIKMRSYNPEGESSG
                     RYREVRDEDDDWSSDEF"
     regulatory      396..398
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             759..788
                     /codon_start=1
                     /label=GGGGS2 linker
                     /translation="GGGGSGGGGS"
     CDS             789..1673
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEIS"
     CDS             1674..1709
                     /codon_start=1
                     /label=GSA linker
                     /translation="GSAGSAAGSGEF"
     CDS             1710..3935
                     /codon_start=1
                     
                     /translation="MESRDHNNPQEGPTSSSGRRAAVEDNHLLIKAVQNEDVDLVQQLL
                     EGGANVNFQEEEGGWTPLHNAVQMSREDIVELLLRHGADPVLRKKNGATPFILAAIAGS
                     VKLLKLFLSKGADVNECDFYGFTAFMEAAVYGKVKALKFLYKRGANVNLRRKTKEDQER
                     LRKGGATALMDAAEKGHVEVLKILLDEMGADVNACDNMGRNALIHALLSSDDSDVEAIT
                     HLLLDHGADVNVRGERGKTPLILAVEKKHLGLVQRLLEQEHIEINDTDSDGKTALLLAV
                     ELKLKKIAELLCKRGASTDCGDLVMTARRNYDHSLVKVLLSHGAKEDFHPPAEDWKPQS
                     SHWGAALKDLHRIYRPMIGKLKFFIDEKYKIADTSEGGIYLGFYEKQEVAVKTFCEGSP
                     RAQREVSCLQSSRENSHLVTFYGSESHRGHLFVCVTLCEQTLEACLDVHRGEDVENEED
                     EFARNVLSSIFKAVQELHLSCGYTHQDLQPQNILIDSKKAAHLADFDKSIKWAGDPQEV
                     KRDLEDLGRLVLYVVKKGSISFEDLKAQSNEEVVQLSPDEETKDLIHRLFHPGEHVRDC
                     LSDLLGHPFFWTWESRYRTLRNVGNESDIKTRKSESEILRLLQPGPSEHSKSFDKWTTK
                     INECVMKKMNKFYEKRGNFYQNTVGDLLKFIRNLGEHIDEEKHKKMKLKIGDPSLYFQK
                     TFPDLVIYVYTKLQNTEYRKHFPQTHSPNKPQCDGAGGASGLASPGC"
     misc_feature    3977..4094
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4143..4653
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             4675..5274
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     LTR             5402..5635
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5707..5828
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      5868..6003
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6024..6042)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6052..6068)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      6210..6665
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        6691..6795
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6796..7656
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7827..8415
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    8703..8724
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8739..8769
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8777..8793
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8801..8817
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8838..8856
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8884..9110
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9111..9291
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9338..9463
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9956..10189
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10374..10418
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      301 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      361 gcgctaccgg tgtcgacgat atctctagag ccaccatggc aaaaaccaat gtgttacctt
      421 ttcgacaaaa tgattcatct agtcattgcc agaagagtgg gtctcctatt tcctcagaag
      481 agcggcggcg cagggataag cagcatcttg atgacatcac agcagctcgg cttctaccac
      541 ttcaccatat gcccacgcag ctgctctcca tagaagaatc cttggcactt cagaaacagc
      601 agaaacagaa ttatgaggag atgcaagcaa agctcgcagc gcaaaaatta gctgaaagac
      661 tgaatattaa aatgcggagt tataatccag aaggggagtc ttcagggaga taccgagaag
      721 taagggatga agatgacgat tggtcctctg atgaattcgg cggaggtggt tctggcggtg
      781 gaggttcagc agaaatcggt actggctttc cattcgaccc ccattatgtg gaagtcctgg
      841 gcgagcgcat gcactacgtc gatgttggtc cgcgcgatgg cacccctgtg ctgttcctgc
      901 acggtaaccc gacctcctcc tacgtgtggc gcaacatcat cccgcatgtt gcaccgaccc
      961 atcgctgcat tgctccagac ctgatcggta tgggcaaatc cgacaaacca gacctgggtt
     1021 atttcttcga cgaccacgtc cgcttcatgg atgccttcat cgaagccctg ggtctggaag
     1081 aggtcgtcct ggtcattcac gactggggct ccgctctggg tttccactgg gccaagcgca
     1141 atccagagcg cgtcaaaggt attgcattta tggagttcat ccgccctatc ccgacctggg
     1201 acgaatggcc agaatttgcc cgcgagacct tccaggcctt ccgcaccacc gacgtcggcc
     1261 gcaagctgat catcgatcag aacgttttta tcgagggtac gctgccgatg ggtgtcgtcc
     1321 gcccgctgac tgaagtcgag atggaccatt accgcgagcc gttcctgaat cctgttgacc
     1381 gcgagccact gtggcgcttc ccaaacgagc tgccaatcgc cggtgagcca gcgaacatcg
     1441 tcgcgctggt cgaagaatac atggactggc tgcaccagtc ccctgtcccg aagctgctgt
     1501 tctggggcac cccaggcgtt ctgatcccac cggccgaagc cgctcgcctg gccaaaagcc
     1561 tgcctaactg caaggctgtg gacatcggcc cgggtctgaa tctgctgcaa gaagacaacc
     1621 cggacctgat cggcagcgag atcgcgcgct ggctgtcgac gctcgagatt tccggatccg
     1681 ctggctccgc tgctggttct ggcgaattca tggagagcag ggatcacaac aacccccagg
     1741 agggacccac gtcctccagc ggtagaaggg ctgcagtgga agacaatcac ttgctgatta
     1801 aagctgttca aaacgaagat gttgacctgg tccagcaatt gctggaaggt ggagccaatg
     1861 ttaatttcca ggaagaggaa gggggctgga cacctctgca taacgcagta caaatgagca
     1921 gggaggacat tgtggaactt ctgcttcgtc atggtgctga ccctgttctg aggaagaaga
     1981 atggggccac gccttttatc ctcgcagcga ttgcggggag cgtgaagctg ctgaaacttt
     2041 tcctttctaa aggagcagat gtcaatgagt gtgattttta tggcttcaca gccttcatgg
     2101 aagccgctgt gtatggtaag gtcaaagccc taaaattcct ttataagaga ggagcaaatg
     2161 tgaatttgag gcgaaagaca aaggaggatc aagagcggct gaggaaagga ggggccacag
     2221 ctctcatgga cgctgctgaa aaaggacacg tagaggtctt gaagattctc cttgatgaga
     2281 tgggggcaga tgtaaacgcc tgtgacaata tgggcagaaa tgccttgatc catgctctcc
     2341 tgagctctga cgatagtgat gtggaggcta ttacgcatct gctgctggac catggggctg
     2401 atgtcaatgt gaggggagaa agagggaaga ctcccctgat cctggcagtg gagaagaagc
     2461 acttgggttt ggtgcagagg cttctggagc aagagcacat agagattaat gacacagaca
     2521 gtgatggcaa aacagcactg ctgcttgctg ttgaactcaa actgaagaaa atcgccgagt
     2581 tgctgtgcaa acgtggagcc agtacagatt gtggggatct tgttatgaca gcgaggcgga
     2641 attatgacca ttcccttgtg aaggttcttc tctctcatgg agccaaagaa gattttcacc
     2701 ctcctgctga agactggaag cctcagagct cacactgggg ggcagccctg aaggatctcc
     2761 acagaatata ccgccctatg attggcaaac tcaagttctt tattgatgaa aaatacaaaa
     2821 ttgctgatac ttcagaagga ggcatctacc tggggttcta tgagaagcaa gaagtagctg
     2881 tgaagacgtt ctgtgagggc agcccacgtg cacagcggga agtctcttgt ctgcaaagca
     2941 gccgagagaa cagtcacttg gtgacattct atgggagtga gagccacagg ggccacttgt
     3001 ttgtgtgtgt caccctctgt gagcagactc tggaagcgtg tttggatgtg cacagagggg
     3061 aagatgtgga aaatgaggaa gatgaatttg cccgaaatgt cctgtcatct atatttaagg
     3121 ctgttcaaga actacacttg tcctgtggat acacccacca ggatctgcaa ccacaaaaca
     3181 tcttaataga ttctaagaaa gctgctcacc tggcagattt tgataagagc atcaagtggg
     3241 ctggagatcc acaggaagtc aagagagatc tagaggacct tggacggctg gtcctctatg
     3301 tggtaaagaa gggaagcatc tcatttgagg atctgaaagc tcaaagtaat gaagaggtgg
     3361 ttcaactttc tccagatgag gaaactaagg acctcattca tcgtctcttc catcctgggg
     3421 aacatgtgag ggactgtctg agtgacctgc tgggtcatcc cttcttttgg acttgggaga
     3481 gccgctatag gacgcttcgg aatgtgggaa atgaatccga catcaaaaca cgaaaatctg
     3541 aaagtgagat cctcagacta ctgcaacctg ggccttctga acattccaaa agttttgaca
     3601 agtggacgac taagattaat gaatgtgtta tgaaaaaaat gaataagttt tatgaaaaaa
     3661 gaggcaattt ctaccagaac actgtgggtg atctgctaaa gttcatccgg aatttgggag
     3721 aacacattga tgaagaaaag cataaaaaga tgaaattaaa aattggagac ccttccctgt
     3781 attttcagaa gacatttcca gatctggtga tctatgtcta cacaaaacta cagaacacag
     3841 aatatagaaa gcatttcccc caaacccaca gtccaaacaa gcctcagtgt gatggagctg
     3901 gtggggccag tgggttggcc agccctgggt gctgagaatt ctcgacctcg agacaaatgg
     3961 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     4021 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     4081 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg ccgcggctcg
     4141 agggggttgg ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc
     4201 tctgggcgtg gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg
     4261 tccgttcgca gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt
     4321 cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa
     4381 acggaagccg cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca
     4441 gcgcgccgac cgcgatgggc tgtggccaat agcggctgct cagcagggcg cgccgagagc
     4501 agcggccggg aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc
     4561 ctgcccgcgc ggtgttccgc attctgcaag cctccggagc gcacgtcggc agtcggctcc
     4621 ctcgttgacc gaatcaccga cctctctccc cagggggatc caccggagct taccatgacc
     4681 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     4741 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     4801 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     4861 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     4921 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     4981 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     5041 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     5101 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     5161 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     5221 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg
     5281 ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc ggtaccttta
     5341 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     5401 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     5461 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     5521 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     5581 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     5641 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     5701 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     5761 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     5821 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     5881 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     5941 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     6001 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     6061 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     6121 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     6181 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     6241 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     6301 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     6361 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     6421 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     6481 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     6541 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     6601 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     6661 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     6721 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     6781 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     6841 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     6901 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     6961 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     7021 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     7081 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     7141 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     7201 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     7261 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     7321 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     7381 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     7441 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     7501 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     7561 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     7621 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     7681 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     7741 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     7801 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     7861 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     7921 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     7981 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     8041 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     8101 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     8161 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     8221 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     8281 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     8341 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     8401 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     8461 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     8521 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     8581 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     8641 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     8701 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     8761 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     8821 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct
     8881 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
     8941 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
     9001 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
     9061 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct
     9121 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     9181 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     9241 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     9301 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     9361 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     9421 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     9481 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     9541 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     9601 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     9661 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     9721 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     9781 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     9841 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     9901 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     9961 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
    10021 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
    10081 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
    10141 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
    10201 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
    10261 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
    10321 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
    10381 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
    10441 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
    10501 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
    10561 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
    10621 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcgat
    10681 cacgagacta gcctcgagcg gccgccccct tcaccgaggg cctatttccc atgattcctt
    10741 catatttgca tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa
    10801 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg
    10861 cagttttaaa attatgtttt aaaatggact atca
//
