LOCUS       Copy_of_Copy_of_Copy_of 9585 bp ds-DNA     circular     10-DEC-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      1..683
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    61..680
                     /label="pBR322_origin"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    566..585
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(972..977)
                     /label="polyA_signal"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(1030..1035)
                     /label="polyA_signal"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    1079..1131
                     /label="3'-IFR"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1121..1125
                     /label="Tol2_consensus"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1130..1151
                     /label="neo_pA_R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(1178..1182)
                     /label="Tol2_consensus"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1267..1272
                     /label="polyA_signal"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    1273..1297
                     /label="blast_seq_F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(1278..1283)
                     /label="polyA_signal"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    1448..1474
                     /label="Puro-R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     gene            complement(1448..2047)
                     /label="puro (variant)"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(1448..2047)
                     /label="PuroR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1894..1913
                     /label="OAEP_rev_5"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1895..1913
                     /label="puro_BstEII"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(1914..1930)
                     /label="Puro_A"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(2037..2041)
                     /label="Tol2_consensus"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(2050..2237)
                     /label="RPL41"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    complement(2238..2673)
                     /label="RPL13A_promoter"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(2646..2650)
                     /label="Tol2_consensus"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(2649..2654)
                     /label="polyA_signal"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     promoter        2682..3849
                     /label="EF1a_promoter"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     exon            2877..2909
                     /label="EEF1A1 exon"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(2978..2982)
                     /label="Tol2_consensus"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3462..3479
                     /label="EF1A_F_2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3602..3620
                     /label="L59Q_A"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3602..3622
                     /label="L59Q_A2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3613..3617
                     /label="Tol2_consensus"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3660..3664
                     /label="Tol2_consensus"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3809..3829
                     /label="EF1a_fwd_primer"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3809..3829
                     /label="EF1a_Forward"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3877..3891
                     /label="NTR_F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3877..3894
                     /label="NTR_F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3903..3923
                     /label="Gateway recomb1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_recomb     3903..3923
                     /label="attB1"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    3906..3925
                     /label="ATTB1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3949..6171
                     /label="pla2g4aa "
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             3949..6216
                     /label="Translation 3949-6216"
                     /translation="MSNIIVEHQYSHRLKLTVVRAENVTKGAFGDLLDTPDPYVELSVPTTPESRKRTRHINNDINPKWNETFEFILDPNQSNVLEVTLMDANYVMDETLGTAKYSLSKLKVAQMEHVTLSIGKTTKVFLDLLLEVCASTDLRFSMTLCDQEKLFMQTRRDRVMLSIKKLLKMENPRFLPSSPREVPTIAILGSGGGFRAMVGFSGVMKALYESGVFDCATYVAGLSGSTWYMSMLYSHPEFPAKGPGDINKELMNRVSNNPLKLLLPQNINRYVKALWKKKSAGQPVTFTDIFGMLIGETLIPGRMNIKLSSLKGKINEGQSPLPLFTCLHVKPDVSELMFADWVEFSPYEIGMAKYGTFMSPGLFGSKFFMGSVVKQYEENPLHFLMGVWGSAFSILFNRVLGVKETTSSSTMEEELEQIKPEHIVGDDSADNEEETQRGGTESADAEDERQRHAQASWVQRMLTSIMGDTTLFTTREGRAGKVHNFMLGLNLNSTLPFSPFSGITHQTSLEEEVDAVTDPDEFERIYEPLDVKSKKIHVVDSGLTFNLPYPLILRCQRGVDLIISFDFSARPSDSSPPFKELLLAEKWARMNKLPFPKIDSKVFDREGLKECYVFKPAKGDKNCPTIIHFVLANINFRNFKAPGVPRDSDKDIEFGDFDIFDEPASPYSTFNFKYNNQAFKRLHDLMEFNTLNNIEVIKEAIKDSILLRRENPARCSVSLSLSEIENKKFLKRDNSIAKRPTGAGGIRPGSMVSKGE"
     misc_feature    3952..3975
                     /label="pr016f cPLA2 f1"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     exon            3964..4045
                     /label="pla2g4aa exon"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    3993..4014
                     /label="cPla2_C2.2_Seq_F_1kb"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(4041..4046)
                     /label="polyA_signal"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(4128..4147)
                     /label="pr044R cPLA2 MO test r"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(4191..4210)
                     /label="pr016r cPLA2 r1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     exon            4195..4308
                     /label="pla2g4aa exon"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     exon            4309..4346
                     /label="pla2g4aa exon"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     exon            4347..4491
                     /label="pla2g4aa exon"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    4386..4407
                     /label="cPla2_C2.1_Seq_F"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    4393..4412
                     /label="pla2g4aa_genotyping_F1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(4409..4413)
                     /label="HIF-1 consensus"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    4521..4526
                     /label="Shine-Dalgarno"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    complement(4560..4581)
                     /label="cPla2_C2.1_Seq_R_1kb"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    4580..4584
                     /label="HIF-1 consensus"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(4604..4623)
                     /label="pla2g4aa_genotyping_R1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(4604..4624)
                     /label="pla2g4aa_genotyping_R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    4628..4654
                     /label="cPla2_C2_E9_Seq_F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(4645..4649)
                     /label="Tol2_consensus"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    4675..4679
                     /label="dcm methylation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(4675..4679)
                     /label="dcm methylation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    4687..4692
                     /label="polyA_signal"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(4750..4769)
                     /label="pla2g4aa_genotyping_R5"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(4830..4850)
                     /label="cPla2_C2_E9_Seq_R"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    4846..4850
                     /label="dcm methylation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(4846..4850)
                     /label="dcm methylation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     exon            4852..4966
                     /label="pla2g4aa exon"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(4930..4934)
                     /label="HIF-1 consensus"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(4959..4978)
                     /label="Pr022r cPLA2g4a r3 mid."
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    4986..4994
                     /label="CEBP_site"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    5026..5030
                     /label="dcm methylation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(5026..5030)
                     /label="dcm methylation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    5183..5188
                     /label="Shine-Dalgarno"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    5235..5240
                     /label="GATA site"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    5243..5248
                     /label="Shine-Dalgarno"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    5259..5264
                     /label="Shine-Dalgarno"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    5307..5311
                     /label="dcm methylation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(5307..5311)
                     /label="dcm methylation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    5393..5419
                     /label="qPCR_pla2g4aa_F "
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    5477..5482
                     /label="Shine-Dalgarno"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    complement(5587..5606)
                     /label="pr021r cPLA2 r"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(5730..5738)
                     /label="CEBP_site"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    5750..5754
                     /label="Tol2_consensus"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(5853..5861)
                     /label="CEBP_site"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    5854..5862
                     /label="CEBP_site"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    5872..5876
                     /label="dcm methylation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(5872..5876)
                     /label="dcm methylation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(5945..5953)
                     /label="CEBP_site"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(5947..5951)
                     /label="Tol2_consensus"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    5971..5991
                     /label="pla2g4aa_genotyping_F4"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    5976..5980
                     /label="dcm methylation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(5976..5980)
                     /label="dcm methylation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(6035..6040)
                     /label="GATA site"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(6154..6162)
                     /label="CEBP_site"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(6170..6211)
                     /label="aroE_mKate2_R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    6172..6192
                     /label="alox12_C"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    6177..6185
                     /label="CEBP_site"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    6179..6201
                     /label="pr047f mk2 ECOR1-f"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    6188..6211
                     /label="mKate2_F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    6190..6194
                     /label="dcm methylation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(6190..6194)
                     /label="dcm methylation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    6199..6222
                     /label="011_mKate2_F_Sequencing"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    6217..6903
                     /label="mKate2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             6217..7005
                     /label="Translation 6217-7005"
                     /translation="ELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKAVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARYCDLPSKLGHRLSRDPAFLYKVGGSGATNFSLLKQAGDVEENPGP"
     misc_feature    complement(6243..6262)
                     /label="pr020r mk2-N1 r"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    6267..6287
                     /label="013_Casp9_seq_F2"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    6381..6385
                     /label="dcm methylation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(6381..6385)
                     /label="dcm methylation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    6429..6433
                     /label="dcm methylation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(6429..6433)
                     /label="dcm methylation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    6509..6513
                     /label="HIF-1 consensus"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(6521..6539)
                     /label="mK2_NLS_seq_R5"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    6525..6529
                     /label="dcm methylation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(6525..6529)
                     /label="dcm methylation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    6540..6544
                     /label="dcm methylation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(6540..6544)
                     /label="dcm methylation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    6546..6564
                     /label="020_MLKL-seqF1"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(6546..6564)
                     /label="020_MLKL-seqF1_RC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    6669..6673
                     /label="dcm methylation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(6669..6673)
                     /label="dcm methylation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    6818..6823
                     /label="Shine-Dalgarno"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    complement(6853..6912)
                     /label="XhoI_mKate2_nostop_AdeI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    6856..6908
                     /label="AdeI_mKate2_nostop_XhoI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    6869..6888
                     /label="pr020f mk2-C1 f"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(6869..6888)
                     /label="pr020f mk2-C1 f_RC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(6906..6929)
                     /label="nostop_colony_PCR_R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(6914..6937)
                     /label="ATTB2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_recomb     complement(6917..6937)
                     /label="attB2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(6917..6937)
                     /label="Gateway Recomb2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    6940..6957
                     /label="mK2_C"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    6940..6959
                     /label="P2A_F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    6940..7005
                     /label="P2A"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    6967..6988
                     /label="NcoI_removal_A"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    6983..6987
                     /label="HIF-1 consensus"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             7006..7062
                     /label="Translation 7006-7062"
                     /translation="KLSLVAAMLLLLSAARAGP"
     misc_feature    complement(7009..7014)
                     /label="GATA site"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    7063..7080
                     /label="QAQA_TS_F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     gene            7063..7776
                     /label="EGFP"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             7063..7827
                     /label="Translation 7063-7827"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRAQASKDEL***"
     misc_feature    complement(7101..7119)
                     /label="pr043r EGFP-N1 rev"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    7104..7108
                     /label="dcm methylation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(7104..7108)
                     /label="dcm methylation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(7105..7126)
                     /label="EGFP-N"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(7106..7126)
                     /label="EGFP-N"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    7126..7148
                     /label="sGFP2_to_mClover3_C_2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(7131..7148)
                     /label="sGFP2_to_mClover3_B_2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    7145..7149
                     /label="HIF-1 consensus"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    7203..7221
                     /label="RhoA_wt_mScarletI_SGFP2_FOR_2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    7229..7233
                     /label="dcm methylation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(7229..7233)
                     /label="dcm methylation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    7262..7266
                     /label="HIF-1 consensus"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    7305..7325
                     /label="5'_KpnI_mClover3"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    7307..7325
                     /label="5’ KpnI_mClover3_2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    7341..7345
                     /label="dcm methylation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(7341..7345)
                     /label="dcm methylation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    7374..7396
                     /label="GFP_CRISPR_site"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(7400..7418)
                     /label="hCD4-for1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    7413..7432
                     /label="CTS40R_For1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(7413..7432)
                     /label="CTS40R_Rev1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    7416..7420
                     /label="dcm methylation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(7416..7420)
                     /label="dcm methylation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(7429..7448)
                     /label="cpYFP_D"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    7431..7467
                     /label="cpYFP_E"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    7454..7459
                     /label="Shine-Dalgarno"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    7470..7474
                     /label="dcm methylation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(7470..7474)
                     /label="dcm methylation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    7474..7494
                     /label="GCamp6s_D_RC "
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(7474..7494)
                     /label="GCamp6s_D"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    7486..7490
                     /label="Tol2_consensus"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    7586..7590
                     /label="HIF-1 consensus"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    7713..7731
                     /label="EGFP-C1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    7713..7734
                     /label="EGFP-C"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(7713..7734)
                     /label="EGFP-C_RC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(7738..7771)
                     /label="cpYFP_B"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    7746..7764
                     /label="pr087f pEGFPC1for"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(7762..7782)
                     /label="eGFP_D"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    7800..7832
                     /label="HindIII_KDEL_F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(7804..7828)
                     /label="KpnI_KDEL_R "
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    7819..7824
                     /label="GATA site"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(7861..7881)
                     /label="BGHR_long_2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(7864..7881)
                     /label="BGHR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(7864..7881)
                     /label="pr086r BGH rev"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(7864..7881)
                     /label="BGH_rev_primer"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(7864..7886)
                     /label="BGH-R_long"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     terminator      7867..8094
                     /label="bGH_PA_terminator"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    complement(7917..7934)
                     /label="BGH_R_2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    7960..7965
                     /label="polyA_signal"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    7991..7995
                     /label="Tol2_consensus"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(8125..8181)
                     /label="5'-ITR"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    8286..8290
                     /label="Tol2_consensus"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    8342..8346
                     /label="Tol2_consensus"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(8543..8548)
                     /label="polyA_signal"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     promoter        8561..8589
                     /label="AmpR_promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    8627..8631
                     /label="Tol2_consensus"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     gene            8631..9491
                     /label="Ampicillin"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    8633..8637
                     /label="Tol2_consensus"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    8719..8723
                     /label="Tol2_consensus"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             8829..9488
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    8934..8938
                     /label="Tol2_consensus"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(8937..8941)
                     /label="Tol2_consensus"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(9034..9038)
                     /label="Tol2_consensus"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(9217..9221)
                     /label="Tol2_consensus"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(9221..9225)
                     /label="Tol2_consensus"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(9312..9317)
                     /label="polyA_signal"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(9507..9511)
                     /label="Tol2_consensus"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(9517..9521)
                     /label="Tol2_consensus"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
ORIGIN
        1 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
       61 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      121 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      181 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      241 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      301 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      361 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      421 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      481 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      541 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      601 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      661 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      721 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      781 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      841 cggatcccta tacagttgaa gtcggaagtt tacatacacc ttagccaaat acatttaaac
      901 tcactttttc acaattcctg acatttaatc ctagtaaaaa ttccctgtct taggtcagtt
      961 aggatcacca ctttatttta agaatgtgaa atatcagaat aatagtagag agaatgattc
     1021 atttcagctt ttatttcttt catcacattc ccagtgggtc agaagtttac atacactcga
     1081 ctcctctgca gaatgcggcg atgtttcggt aaggggtccc gagtagctag ttcatggcag
     1141 ccagcatatg gcatatgttg ccaaactcta aaccaaatac tcattctgat gttttaaatg
     1201 atttgccctc ccatatgtcc ttccgagtga gagacacaaa aaattccaac acactattgc
     1261 aatgaaaata aatttccttt attagccaga agtcagatgc tcaaggggct tcatgatgtc
     1321 cccataattt ttggcagagg gaaaaagatc tcagtggtat ttgtgagcca gggcattggc
     1381 cacaccagcc accaccttct gataggcagc ctgcacctga ggagtgaatt cacgacaggc
     1441 cttcgaatca ggcaccgggc ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct
     1501 cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg
     1561 aggtctccag gaaggcgggc accccggcgc gctcggccgc ctccactccg gggagcacga
     1621 cggcgctgcc cagacccttg ccctggtggt cgggcgagac gccgacggtg gccaggaacc
     1681 acgcgggctc cttgggccgg tgcggcgcca ggaggccttc catctgttgc tgcgcggcca
     1741 gccgggaacc gctcaactcg gccatgcgcg ggccgatctc ggcgaacacc gcccccgctt
     1801 cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc gtcgtccgcg acccacacct
     1861 tgccgatgtc gagcccgacg cgcgtgagga agagttcttg cagctcggtg acccgctcga
     1921 tgtggcggtc cggatcgacg gtgtggcgcg tggcggggta gtcggcgaac gcggcggcga
     1981 gggtgcgtac ggccctgggg acgtcgtcgc gggtggcgag gcgcaccgtg ggcttgtact
     2041 cggtcatggt ggcctcaggt gcagaggttt ctacagggaa agagagaaag gctacagtta
     2101 gtttgctgtg atgatgctaa gtgccgaggt cagatgtcta tggattgtac acggaatccc
     2161 aacgcaggaa ccaaaaaaca ctcacccaaa aaaatggcgc taaggccgag agaaaggcga
     2221 agttccgtct acggctatat accttgaaac aggggcggca gaagtgacgc aacgcggttg
     2281 cgcggacggc ttcctgtgtt ttcattggtc gaagcgttct gcgcgtgcgc agagattccc
     2341 cagggaaggc gggccctctt ccgaatgtcc aatccggaca ttcttccggt tggacctccg
     2401 cggtcagcgc tgtgcccggc gagagatcac gtggggcgcg gaggcggtgc tgctggggca
     2461 cggccgtcca gcctcggcgg ccatattttt gaggggctgt tcatctcgtt cacacgctct
     2521 gtccgccatg tttgtgagtg gaagcgccat taccccttca agcgactgaa ggctgcaggg
     2581 cctctggtgg cccgcatggg gagaccagac ccgccaggcc cgcctttccg cactcagtcc
     2641 gggcttactt tattttgtga gacagggtct cgcctagagg ctccggtgcc cgtcagtggg
     2701 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2761 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2821 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2881 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2941 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacgccc ctggctgcag
     3001 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc
     3061 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcttgggcg ctggggccgc
     3121 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
     3181 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
     3241 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
     3301 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
     3361 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
     3421 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
     3481 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag
     3541 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca
     3601 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg
     3661 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccatactgag
     3721 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
     3781 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
     3841 cttccattta aggtgtcgtg aaaactaccc caagctggcc tctgaggcca ccatggacgc
     3901 gtcaagtttg tacaaaaaag caggctggac ggatctatcg atgccacaat gtccaacatt
     3961 atagttgagc atcagtattc tcatagactg aaattgactg tggtgagagc tgaaaatgta
     4021 actaaaggag catttgggga tttattggac accccggacc cttatgttga gctgtcagtg
     4081 ccaaccacac cagagtcaag aaaacgaacc cgccacatta acaatgacat taaccccaag
     4141 tggaacgaga cctttgagtt catattggat cccaatcaat ccaatgtttt ggaggtgaca
     4201 ctgatggatg ccaattatgt catggatgaa actcttggca cagcaaaata ttccctctca
     4261 aaactcaaag tggcacaaat ggagcatgtg accctctcta tcggcaagac aaccaaagtg
     4321 ttcctcgact tgctcttgga agtttgcgca tccacagacc tgcgcttcag catgacactg
     4381 tgtgatcaag agaagctgtt catgcagaca cgcagagaca gggtcatgct cagcataaag
     4441 aagcttctta aaatggaaaa ccctcgtttc cttccatcct caccaagaga ggttcccacc
     4501 atcgctattc tgggctctgg aggaggtttt cgtgcaatgg tgggcttctc tggtgtgatg
     4561 aaggctttgt atgaatctgg cgtgtttgac tgtgcaacat atgtggcagg actttcagga
     4621 tcaacatggt atatgtctat gctgtactct catccagagt ttcctgctaa aggcccaggc
     4681 gacattaata aagagctcat gaacagagtc agtaataacc cactcaaact gctcctgccc
     4741 caaaacatca accgctacgt caaagcactg tggaagaaga aatctgctgg acagccggtc
     4801 accttcactg acatcttcgg gatgttgata ggagagacgc ttattccagg gagaatgaac
     4861 attaagctga gctcattgaa agggaaaatt aatgaaggtc agagccctct tcctctcttc
     4921 acctgtctgc acgtcaaacc tgacgtctct gagctcatgt ttgcagattg ggtggagttc
     4981 agcccttatg aaattggcat ggccaaatat ggcaccttca tgtctcctgg gctgtttggc
     5041 agcaaattct tcatgggaag tgttgtgaaa caatatgagg aaaacccctt acatttcctg
     5101 atgggtgtgt ggggaagtgc tttttccatc ctctttaacc gggttttggg tgtgaaggag
     5161 acgaccagca gtagcactat ggaggaggag ctggagcaaa ttaaaccaga gcacattgtg
     5221 ggtgacgatt ctgctgataa cgaggaggaa actcaaagag gaggcacaga gtctgcagat
     5281 gcagaggacg agcgccagcg tcatgcccag gccagctggg tacagcgcat gttgacctcc
     5341 atcatgggcg acaccactct gttcaccact cgtgagggcc gcgcaggcaa agtgcacaac
     5401 ttcatgctgg gactgaacct gaactcaacc ctgcccttct ctcccttcag cggcatcaca
     5461 caccagacct cgctggagga ggaagtggac gctgttactg atccggatga gtttgagcga
     5521 atctatgagc ctctggatgt gaagagcaag aaaatccatg tggtggacag tggcttgacc
     5581 tttaacctgc cctaccctct cattctgcgg tgtcagagag gagtcgacct gattatttca
     5641 ttcgacttct cagcccgacc cagtgactcc agcccaccgt tcaaagagtt actgctggct
     5701 gaaaaatggg cccgtatgaa taagctgccc ttcccgaaga ttgattcgaa agtattcgac
     5761 agagagggtc taaaagagtg ctatgtgttc aagcctgcta aaggagacaa gaactgcccc
     5821 accatcattc attttgtctt ggccaacatc aacttccgga attttaaagc ccctggagtt
     5881 cctcgagatt cagacaagga catagagttt ggtgactttg acatatttga cgagccagcc
     5941 tccccttact ccactttcaa cttcaaatac aacaaccagg ccttcaagag acttcatgac
     6001 ctcatggagt tcaatacgct taacaacatc gaggttatca aagaagccat taaagacagc
     6061 attctgctga gacgagagaa cccagcgcga tgttcggttt ccctttcgct cagtgaaatc
     6121 gaaaacaaga agttcctgaa acgagacaac agcattgcaa aacgacccac cggagctgga
     6181 ggaattcgtc ctggatctat ggtgtctaag ggcgaagagc tgattaagga gaacatgcac
     6241 atgaagctgt acatggaggg caccgtgaac aaccaccact tcaagtgcac atccgagggc
     6301 gaaggcaagc cctacgaggg cacccagacc atgagaatca aggccgtcga gggcggccct
     6361 ctccccttcg ccttcgacat cctggctacc agcttcatgt acggcagcaa aaccttcatc
     6421 aaccacaccc agggcatccc cgacttcttt aagcagtcct tccctgaggg cttcacatgg
     6481 gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcctc
     6541 caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttccc atccaacggc
     6601 cctgtgatgc agaagaaaac actcggctgg gaggcctcca ccgagaccct gtaccccgct
     6661 gacggcggcc tggaaggcag agccgacatg gccctgaagc tcgtgggcgg gggccacctg
     6721 atctgcaact tgaagaccac atacagatcc aagaaacccg ctaagaacct caagatgccc
     6781 ggcgtctact atgtggacag aagactggaa agaatcaagg aggccgacaa agagacctac
     6841 gtcgagcagc acgaggtggc tgtggccaga tactgcgacc tccctagcaa actggggcac
     6901 agactgTctc gagacccagc tttcttgtac aaagtggggg gatccggagc cacgaacttc
     6961 tctctgttaa agcaagcagg agacgtggaa gaaaaccccg gCcctAAGTT ATCATTAGTT
     7021 GCAGCTATGT TACTATTGTT AAGCGCGGCG AGAGCAgGTC CGGTGAGCAA GGGCGAGGAG
     7081 CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG
     7141 TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC
     7201 ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTAC
     7261 GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC
     7321 GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC
     7381 AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG
     7441 GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC
     7501 AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGT GAACTTCAAG
     7561 ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC
     7621 CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC
     7681 CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC
     7741 GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC TCGAGCTCAA
     7801 GCTTCCAAGG ACGAGCTATG ATAATAGGGT ACCGCGGGCC CGGGtctaga gctcgctgat
     7861 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     7921 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     7981 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     8041 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     8101 aggcggaaag aaccagctgg gcctactaga tagcggaccc cttaccgaaa catcgccgca
     8161 ttctgcagag gagtcgaact tagtgtatgt aaacttctga cccactggaa ttgtgataca
     8221 gtgaattata agtgaaataa tctgtctgta aacaattgtt ggaaaaatga cttgtgtcat
     8281 gcacaaagta gatgtcctaa ctgacttgcc aaaactattg tttgttaaca agaaatttgt
     8341 ggagtagttg aaaaacgagt tttaatgact ccaacttaag tgtatgtaaa cttccgactt
     8401 caactgtata gggatccaat tttaacaaaa tattaacgct tacaatttcc tgatgcggta
     8461 ttttctcctt acgcatctgt gcggtatttc acaccgcatc aggtggcact tttcggggaa
     8521 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     8581 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     8641 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     8701 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     8761 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     8821 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     8881 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     8941 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     9001 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     9061 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     9121 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     9181 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     9241 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     9301 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     9361 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     9421 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     9481 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     9541 atttttaatt taaaaggatc taggtgaaga tcctttttga taatc
//