LOCUS       Copy_of_pSB-CMV-MCS-Pur 9262 bp ds-DNA     circular     10-DEC-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        7..594
                     /label="CMV\promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="invitrogen: 2180000 vntifkey: 29"
     promoter        86..389
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        390..593
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    544..564
                     /label="CMV-Forward"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    544..564
                     /label="CMV\forward\primer"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="invitrogen: 1930000 vntifkey: 27"
     misc_feature    546..563
                     /label="CMV_F2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    549..564
                     /label="pr086f CMV promoter f"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        638..654
                     /label="T7\promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="invitrogen: 2370000 vntifkey: 30"
     misc_feature    638..657
                     /label="T7"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     638..657
                     /label="T7"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    638..657
                     /label="T7\primer"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="invitrogen: 2100000 vntifkey: 27"
     misc_feature    682..699
                     /label="011_mTq2-no_RS_F"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    complement(682..699)
                     /label="011_mTq2-no_RS_F_RC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             682..1389
                     /label="mCherry"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="product: monomeric derivative of DsRed fluorescent protein(Shaner et al., 2004) note: mammalian codon-optimized translation: MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK"
     CDS             682..4269
                     /label="Translation 682-4269"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYKSGLRSMPGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAGRTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRSSNSRVGSGATNFSLLKQAGDVEENPGPRSMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKRSMASAACTPAGQQSLSRGTSSTPLSPARISRLQEKEELQQLNDRLAAYIDTVRSLESENSVLQIQIREREDVRNRELTGLRTLYETELADARKALDDTARERARLQIELGKIKSEQEQLQHTYAKKDADLTSSQNRLKDVEALLNAKEAALNTAVSERKTLENTLSDLQIRVQELEGGLISAKKQLSDETLLRVDLENRCQSLMEELEFRKNMFEEEIKDTRRRHETRLVEVDSGRQMEYEFKLAQALTDMRQQHDEQVKLYKEEMEQTYVSKLENIRLSSEMNSSSASMAREELRESTLRVESLAGQLANLQKEARAWQDRINELEGALSHEKDLSRRLLAEKEREIAEIRAKMQQQLDEYEQLLDVKLALDMEINAYRKLLEGEEERLKLSPSPSSRVTVSRASSSRSVRTTRGKRKRVDVEESEASSSVSIAHSASATGNVSIDELDVDGKFIRLHNNSEQDQPMAGFQLTRTIGEVTATYKFTAKYNLKAGQKVTIWASNAGISSNPPADLIWKNQPSWGTGENIKVALLSPAGEEVAVRSTVFKTAVEDEDDDEEEEKEAEVIEEHFYHQQGDPRAAKRGCLIM*"
     misc_feature    685..702
                     /label="QAQA_TS_F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    827..845
                     /label="mCherry-R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     complement(827..845)
                     /label="mCherry-R"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: mCherry, reverse primer"
     primer_bind     1095..1114
                     /label="mCherry-F"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: mCherry, forward primer"
     misc_feature    1095..1114
                     /label="mCherry-F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(1366..1389)
                     /label="TS+Scarlet_I_AQAQ_R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(1375..1395)
                     /label="eGFP_D"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1405..1692
                     /label="Sec 61 Beta"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1708..1773
                     /label="P2A\peptide"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="vntifkey: 21"
     misc_feature    1724..1741
                     /label="AatII_blast_R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1780..1797
                     /label="011_mTq2-no_RS_F"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    complement(1780..1797)
                     /label="011_mTq2-no_RS_F_RC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1780..2496
                     /label="EGFP"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="vntifkey: 21"
     misc_feature    1783..1800
                     /label="QAQA_TS_F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(1821..1839)
                     /label="pr043r EGFP-N1 rev"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(1825..1846)
                     /label="EGFP-N"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(1826..1846)
                     /label="EGFP-N"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1846..1868
                     /label="sGFP2_to_mClover3_C_2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(1851..1868)
                     /label="sGFP2_to_mClover3_B_2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1923..1941
                     /label="RhoA_wt_mScarletI_SGFP2_FOR_2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2025..2045
                     /label="5'_KpnI_mClover3"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2027..2045
                     /label="5’ KpnI_mClover3_2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(2120..2138)
                     /label="hCD4-for1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2133..2152
                     /label="CTS40R_For1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(2133..2152)
                     /label="CTS40R_Rev1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(2149..2168)
                     /label="cpYFP_D"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2151..2187
                     /label="cpYFP_E"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2194..2214
                     /label="GCamp6s_D_RC "
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(2194..2214)
                     /label="GCamp6s_D"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2433..2451
                     /label="EGFP-C1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2433..2454
                     /label="EGFP-C"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(2433..2454)
                     /label="EGFP-C_RC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(2458..2491)
                     /label="cpYFP_B"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2466..2484
                     /label="pr087f pEGFPC1for"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    2494..2519
                     /label="pr046f bglII-LMNB1 f"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2503..4269
                     /label="LMNB1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="vntifkey: 21"
     misc_feature    2861..2887
                     /label="lmnb1-seq-1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(2863..2890)
                     /label="lmnb1_NdeI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(2873..2890)
                     /label="lmnb1_NdeI_2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3367..3386
                     /label="lmnb1-seq-2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(3612..3629)
                     /label="lmnb1_AfeI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(4298..4318)
                     /label="BGHR_long_2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(4301..4318)
                     /label="BGHR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(4301..4318)
                     /label="BGH\reverse\primer"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="invitrogen: 1910000 vntifkey: 27"
     misc_feature    complement(4301..4323)
                     /label="BGH-R_long"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_site      4307..4531
                     /label="BGH\pA"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="invitrogen: 1870000 vntifkey: 26"
     misc_feature    complement(4354..4371)
                     /label="BGH_R_2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(4557..4578)
                     /label="pr081r common rev"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(4561..4581)
                     /label="pBABE_3_primer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="vntifkey: 21"
     misc_feature    complement(4567..4782)
                     /label="SV40_enhancer"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="vntifkey: 21"
     promoter        4579..4847
                     /label="SV40_promoter"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="vntifkey: 30"
     promoter        4579..4895
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     promoter        4699..4895
                     /label="SV40 early promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     promoter        4699..4901
                     /label="SV40_promoter"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="vntifkey: 30"
     rep_origin      4746..4823
                     /label="SV40_origin"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="vntifkey: 33"
     misc_feature    4808..4827
                     /label="SV40-promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    4808..4827
                     /label="SV40pro_F_primer"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="vntifkey: 21"
     misc_feature    4808..4827
                     /label="SV40pro-F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             4923..5522
                     /label="puro(variant)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="gene: puro(variant) vntifkey: 4"
     misc_feature    5040..5056
                     /label="Puro_A"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(5057..5075)
                     /label="puro_BstEII"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(5057..5076)
                     /label="OAEP_rev_5"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(5496..5522)
                     /label="Puro-R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(5536..5557)
                     /label="mK2_F2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(5579..5609)
                     /label="mK2_F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    5590..5609
                     /label="SV40pA-R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(5590..5609)
                     /label="SV40-pA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    5635..5640
                     /label="polyA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="vntifkey: 25"
     misc_feature    5665..5687
                     /label="pr087r pEGFPC1rev"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    5668..5688
                     /label="065r mTq2 r"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    5680..5696
                     /label="EGFP-C-REV"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    5833..6060
                     /label="IR-DR(R)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="vntifkey: 44"
     misc_feature    complement(6119..6136)
                     /label="T3"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(6119..6138)
                     /label="T3"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     complement(6119..6138)
                     /label="T3"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        complement(6120..6139)
                     /label="T3"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="vntifkey: 30"
     promoter        complement(6121..6139)
                     /label="T3 promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(6156..6171)
                     /label="M13R1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     complement(6156..6176)
                     /label="M13-rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(6159..6176)
                     /label="M13R2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(6160..6176)
                     /label="M13R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(6173..6195)
                     /label="M13pUC-Rev"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(6174..6193)
                     /label="M13-48REV"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(6182..6204)
                     /label="lacO"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_binding    complement(6182..6204)
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     promoter        complement(6208..6238)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     rep_origin      6380..7260
                     /label="ori"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="vntifkey: 33"
     rep_origin      complement(6544..7226)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(6642..6661)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             complement(7324..7983)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(7324..8181)
                     /label="Amp"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="vntifkey: 4"
     rep_origin      8312..8768
                     /label="F1\ori"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="vntifkey: 33"
     rep_origin      8330..8636
                     /label="F1 ori"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             complement(8769..8837)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    8889..8905
                     /label="M13F1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    8894..8916
                     /label="M13pUC-Fwd"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     8908..8925
                     /label="M13-fwd"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    8908..8925
                     /label="M13F2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    8909..8924
                     /label="M13F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     promoter        8934..8954
                     /label="T7"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="vntifkey: 30"
     misc_feature    8935..8954
                     /label="T7"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(8979..9205)
                     /label="IR-DR(L)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="vntifkey: 44"
ORIGIN
        1 aagcttgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagctgg ctagcgccac catggtgagc aagggcgagg aggataacat ggccatcatc
      721 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
      781 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
      841 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
      901 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
      961 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     1021 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     1081 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
     1141 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
     1201 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
     1261 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
     1321 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     1381 ctgtacaagt ccggactcag atctatgcct ggtccgaccc ccagtggcac taacgtggga
     1441 tcctcagggc gctctcccag caaagcagtg gccgcccggg cggcgggatc cactgtccgg
     1501 cagaggaaaa atgccagctg tgggacaagg agtgcaggcc gcacaacctc ggcaggcacc
     1561 ggggggatgt ggcgattcta cacagaagat tcacctgggc tcaaagttgg ccctgttcca
     1621 gtattggtta tgagtcttct gttcatcgct tctgtattta tgttgcacat ttggggcaag
     1681 tacactcgtt cgtcgaattc ccgggtcgga agcggagcta ctaacttcag cctgctgaag
     1741 caggctggag acgtggagga gaaccctgga cctagatcca tggtgagcaa gggcgaggag
     1801 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     1861 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     1921 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     1981 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     2041 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     2101 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     2161 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     2221 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     2281 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     2341 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     2401 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     2461 gccgggatca ctctcggcat ggacgagctg tacaagagat ctatggcgag cgcggcgtgt
     2521 actccggccg ggcagcagtc gttgtcccgc ggcaccagca gcactccact gagccccgcg
     2581 cggatctcca ggctgcaaga gaaagaggag ctgcagcagc tcaatgaccg gcttgccgcc
     2641 tacatcgaca cagtccgtag tctggagtcc gagaacagcg tcctgcaaat acagatcaga
     2701 gaacgagagg atgtgcggaa ccgcgagctc acggggctca gaacactcta cgaaaccgag
     2761 ctggcggacg cacggaaggc gcttgatgac acggcacggg agcgcgcacg attgcagatt
     2821 gaactgggca agattaaaag cgagcaagag cagctacagc acacatatgc caagaaagat
     2881 gctgacctca ccagctctca gaaccgacta aaagatgttg aagccctgtt gaacgctaaa
     2941 gaagctgcac taaacacagc cgtctctgaa aggaagactc tggaaaacac tctatctgat
     3001 ctgcagatcc gtgtccagga gttggaagga gggttgatct ccgctaagaa gcagctgtca
     3061 gatgagaccc tgctgcgtgt ggatctggag aaccgctgtc agagccttat ggaggaactt
     3121 gaatttagga aaaacatgtt tgaagaggaa atcaaagata cacgtcgtag acatgagacc
     3181 cgtcttgtcg aggtggattc aggtcgtcag atggagtatg agtttaaact ggctcaggct
     3241 ctgactgaca tgagacagca gcatgatgag caagtcaaac tttataagga ggagatggag
     3301 cagacatacg tgtccaagct ggagaacatt cgtttgtcat ctgagatgaa cagctcttca
     3361 gctagtatgg cccgtgagga gcttcgagag tctacactga gagttgagag tttggcaggt
     3421 caacttgcaa atctgcagaa agaggctcgt gcctggcagg atcgcattaa tgaactggag
     3481 ggggctctta gccatgagaa agatttaagc cgtaggttgc tggcagagaa agagcgcgag
     3541 attgctgaga tcagagccaa gatgcagcag cagctggatg agtatgagca actgctggac
     3601 gtcaaactag cgctggacat ggagatcaat gcttacagaa agcttctgga gggagaagaa
     3661 gaaaggttga aactgtctcc aagtccttca tctcgtgtga ctgtgtcacg agcatcctcc
     3721 agtcgcagtg ttcgtactac ccgtggcaag agaaagcgtg tggatgtgga ggagtcggag
     3781 gcaagcagca gtgtcagtat cgctcattct gcatctgcca ctggaaacgt ctccatcgat
     3841 gagctggatg tagatgggaa gttcatccgc ttgcacaaca actctgagca ggaccagccg
     3901 atggcaggat ttcagctgac caggactatt ggagaagtta ctgcaacata taaattcact
     3961 gcaaaataca atctgaaggc tggacagaaa gtgacgatct gggcatctaa tgcaggcata
     4021 agctccaacc ctcctgctga cctgatatgg aagaaccagc cttcatgggg gactggggag
     4081 aacataaaag tggctctttt gagcccagca ggagaggaag tagctgtgag aagcacagta
     4141 ttcaagactg cagtggaaga tgaagacgac gatgaggagg aagaaaagga agctgaagtc
     4201 attgaggagc atttctatca ccagcaggga gatccacgcg ctgctaagag aggatgtttg
     4261 atcatgtaat ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     4321 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     4381 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     4441 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     4501 aggcatgctg gggatgcggt gggctctatg gaccggactg aggcggaaag aaccagctgt
     4561 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4621 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     4681 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     4741 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     4801 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     4861 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct agattttctg accctagcca
     4921 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg
     4981 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg
     5041 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     5101 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     5161 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     5221 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     5281 agcccgcgtg gttcctggcc accgtcggcg tgtcgcccga ccaccagggc aagggtctgg
     5341 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     5401 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     5461 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     5521 gaggatctcg agctcgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     5581 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     5641 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     5701 gttttttaaa gcaagtaaaa cctctacaaa tgtggtactt aagagggatc gataaggatc
     5761 tagcttgtgg aaggctactc gaaatgtttg acccaagtta aacaatttaa aggcaatgct
     5821 accaaatact aattgagtgt atgtaaactt ctgacccact gggaatgtga tgaaagaaat
     5881 aaaagctgaa atgaatcatt ctctctacta ttattctgat atttcacatt cttaaaataa
     5941 agtggtgatc ctaactgacc taagacaggg aatttttact aggattaaat gtcaggaatt
     6001 gtgaaaaagt gagtttaaat gtatttggct aaggtgtatg taaacttccg acttcaactg
     6061 tatagggatc ctctagctag agtcgacctc gagggggggc ccggtaccca gcttttgttc
     6121 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     6181 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     6241 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     6301 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     6361 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     6421 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     6481 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6541 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6601 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6661 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6721 cgcctttctc ccttcgggaa ccgtggcgct ttctcatagc tcacgctgta ggtatctcag
     6781 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     6841 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     6901 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     6961 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     7021 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     7081 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7141 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7201 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     7261 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     7321 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     7381 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     7441 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     7501 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7561 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     7621 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7681 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7741 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaac ttggccgcag tgttatcact
     7801 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     7861 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     7921 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     7981 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     8041 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     8101 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     8161 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     8221 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     8281 tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc
     8341 ggcgggagtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     8401 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     8461 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     8521 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     8581 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     8641 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     8701 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     8761 tacaatttcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     8821 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     8881 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac
     8941 gactcactat agggcgaatt ggagctcgga tccctataca gttgaagtcg gaagtttaca
     9001 tacacttaag ttggagtcat taaaactcgt ttttcaacta ctccacaaat ttcttgttaa
     9061 caaacaatag ttttggcaag tcagttagga catctacttt gtgcatgaca caagtcattt
     9121 ttccaacaat tgtttacaga cagattattt cacttataat tcactgtatc acaattccag
     9181 tgggtcagaa gtttacatac actaagttga ctgtgccttt aaacagcttg gaaaattcca
     9241 gaaaatgatg tcatggcttt ag
//