LOCUS       pSB-CMV-MCS-Puro_ArfGAP 9190 bp ds-DNA     circular     10-DEC-2025
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name:
            psb-cmv-mcs-puro-arfgap1_alps-mkate2-3nls-p2a-egfp-lmnb1-c963.gb
FEATURES             Location/Qualifiers
     promoter        7..594
                     /label="CMV\promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="invitrogen: 2180000 vntifkey: 29"
     promoter        86..389
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        390..593
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    544..564
                     /label="CMV\forward\primer"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="invitrogen: 1930000 vntifkey: 27"
     promoter        638..654
                     /label="T7\promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="invitrogen: 2370000 vntifkey: 30"
     misc_feature    638..657
                     /label="T7\primer"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="invitrogen: 2100000 vntifkey: 27"
     primer_bind     638..657
                     /label="T7"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    682..798
                     /label="ArfGAP1_ALPS"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="vntifkey: 21"
     CDS             682..798
                     /label="Translation 682-798"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MDDFLNSAMSSLYSGWSSFTTGASKFASAAKEGATKFGS"
     misc_feature    808..1515
                     /label="mKate2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="vntifkey: 21"
     CDS             808..1629
                     /label="Translation 808-1629"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKAVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARYCDLPSKLGHKLTGLRSRAQASNSRVDPKKKRKVDPKKKRKVDPKKKRKV"
     misc_feature    1558..1629
                     /label="3NLS"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="vntifkey: 21"
     CDS             1630..4203
                     /label="Translation 1630-4203"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="SGGSGATNFSLLKQAGDVEENPGPRSMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKRSMASAACTPAGQQSLSRGTSSTPLSPARISRLQEKEELQQLNDRLAAYIDTVRSLESENSVLQIQIREREDVRNRELTGLRTLYETELADARKALDDTARERARLQIELGKIKSEQEQLQHTYAKKDADLTSSQNRLKDVEALLNAKEAALNTAVSERKTLENTLSDLQIRVQELEGGLISAKKQLSDETLLRVDLENRCQSLMEELEFRKNMFEEEIKDTRRRHETRLVEVDSGRQMEYEFKLAQALTDMRQQHDEQVKLYKEEMEQTYVSKLENIRLSSEMNSSSASMAREELRESTLRVESLAGQLANLQKEARAWQDRINELEGALSHEKDLSRRLLAEKEREIAEIRAKMQQQLDEYEQLLDVKLALDMEINAYRKLLEGEEERLKLSPSPSSRVTVSRASSSRSVRTTRGKRKRVDVEESEASSSVSIAHSASATGNVSIDELDVDGKFIRLHNNSEQDQPMAGFQLTRTIGEVTATYKFTAKYNLKAGQKVTIWASNAGISSNPPADLIWKNQPSWGTGENIKVALLSPAGEEVAVRSTVFKTAVEDEDDDEEEEKEAEVIEEHFYHQQGDPRAAKRGCLIM*SR"
     misc_feature    1636..1701
                     /label="P2A\peptide"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="vntifkey: 21"
     misc_feature    1708..2424
                     /label="EGFP"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="vntifkey: 21"
     misc_feature    2431..4197
                     /label="LMNB1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="vntifkey: 21"
     misc_feature    complement(4229..4246)
                     /label="BGH\reverse\primer"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="invitrogen: 1910000 vntifkey: 27"
     polyA_site      4235..4459
                     /label="BGH\pA"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="invitrogen: 1870000 vntifkey: 26"
     misc_feature    complement(4489..4509)
                     /label="pBABE_3_primer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="vntifkey: 21"
     misc_feature    complement(4495..4710)
                     /label="SV40_enhancer"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="vntifkey: 21"
     promoter        4507..4775
                     /label="SV40_promoter"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="vntifkey: 30"
     promoter        4507..4823
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     promoter        4627..4823
                     /label="SV40 early promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     promoter        4627..4829
                     /label="SV40_promoter"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="vntifkey: 30"
     rep_origin      4674..4751
                     /label="SV40_origin"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="vntifkey: 33"
     misc_feature    4736..4755
                     /label="SV40pro_F_primer"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="vntifkey: 21"
     CDS             4851..5450
                     /label="puro(variant)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="gene: puro(variant) vntifkey: 4"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     CDS             4851..5450
                     /label="CDS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     polyA_signal    5563..5568
                     /label="polyA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="vntifkey: 25"
     misc_feature    5761..5988
                     /label="IR-DR(R)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="vntifkey: 44"
     primer_bind     complement(6047..6066)
                     /label="T3"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        complement(6048..6067)
                     /label="T3"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="vntifkey: 30"
     promoter        complement(6049..6067)
                     /label="T3 promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     complement(6084..6104)
                     /label="M13-rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_binding    complement(6110..6132)
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     promoter        complement(6136..6166)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     rep_origin      6308..7188
                     /label="ori"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="vntifkey: 33"
     rep_origin      complement(6472..7154)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             complement(7252..7911)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(7252..8109)
                     /label="Amp"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="vntifkey: 4"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAAKLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     CDS             complement(7252..8109)
                     /label="CDS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAAKLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     rep_origin      8240..8696
                     /label="F1\ori"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="vntifkey: 33"
     rep_origin      8258..8564
                     /label="F1 ori"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             complement(8697..8765)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     8836..8853
                     /label="M13-fwd"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        8862..8882
                     /label="T7"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="vntifkey: 30"
     misc_feature    complement(8907..9133)
                     /label="IR-DR(L)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="vntifkey: 44"
ORIGIN
        1 aagcttgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagctgg ctagcgccac catggatgat tttctgaaca gcgcgatgag cagcctgtat
      721 agcggctgga gcagctttac caccggcgcg agcaaatttg cgagcgcggc gaaagaaggc
      781 gcgaccaaat ttggcagcgg accggtcatg gtgtctaagg gcgaagagct gattaaggag
      841 aacatgcaca tgaagctgta catggagggc accgtgaaca accaccactt caagtgcaca
      901 tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggccgtcgag
      961 ggcggccctc tccccttcgc cttcgacatc ctggctacca gcttcatgta cggcagcaaa
     1021 accttcatca accacaccca gggcatcccc gacttcttta agcagtcctt ccctgagggc
     1081 ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac
     1141 accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt gaacttccca
     1201 tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggcctccac cgagaccctg
     1261 taccccgctg acggcggcct ggaaggcaga gccgacatgg ccctgaagct cgtgggcggg
     1321 ggccacctga tctgcaactt gaagaccaca tacagatcca agaaacccgc taagaacctc
     1381 aagatgcccg gcgtctacta tgtggacaga agactggaaa gaatcaagga ggccgacaaa
     1441 gagacctacg tcgagcagca cgaggtggct gtggccagat actgcgacct ccctagcaaa
     1501 ctggggcaca aactgaccgg actcagatct cgagctcaag cttcgaattc ccgggtcgat
     1561 ccaaaaaaga agagaaaggt agatccaaaa aagaagagaa aggtagatcc aaaaaagaag
     1621 agaaaggtat ccggtggaag cggagctact aacttcagcc tgctgaagca ggctggagac
     1681 gtggaggaga accctggacc tagatccatg gtgagcaagg gcgaggagct gttcaccggg
     1741 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     1801 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     1861 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     1921 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     1981 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     2041 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     2101 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     2161 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     2221 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     2281 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     2341 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     2401 ctcggcatgg acgagctgta caagagatct atggcgagcg cggcgtgtac tccggccggg
     2461 cagcagtcgt tgtcccgcgg caccagcagc actccactga gccccgcgcg gatctccagg
     2521 ctgcaagaga aagaggagct gcagcagctc aatgaccggc ttgccgccta catcgacaca
     2581 gtccgtagtc tggagtccga gaacagcgtc ctgcaaatac agatcagaga acgagaggat
     2641 gtgcggaacc gcgagctcac ggggctcaga acactctacg aaaccgagct ggcggacgca
     2701 cggaaggcgc ttgatgacac ggcacgggag cgcgcacgat tgcagattga actgggcaag
     2761 attaaaagcg agcaagagca gctacagcac acatatgcca agaaagatgc tgacctcacc
     2821 agctctcaga accgactaaa agatgttgaa gccctgttga acgctaaaga agctgcacta
     2881 aacacagccg tctctgaaag gaagactctg gaaaacactc tatctgatct gcagatccgt
     2941 gtccaggagt tggaaggagg gttgatctcc gctaagaagc agctgtcaga tgagaccctg
     3001 ctgcgtgtgg atctggagaa ccgctgtcag agccttatgg aggaacttga atttaggaaa
     3061 aacatgtttg aagaggaaat caaagataca cgtcgtagac atgagacccg tcttgtcgag
     3121 gtggattcag gtcgtcagat ggagtatgag tttaaactgg ctcaggctct gactgacatg
     3181 agacagcagc atgatgagca agtcaaactt tataaggagg agatggagca gacatacgtg
     3241 tccaagctgg agaacattcg tttgtcatct gagatgaaca gctcttcagc tagtatggcc
     3301 cgtgaggagc ttcgagagtc tacactgaga gttgagagtt tggcaggtca acttgcaaat
     3361 ctgcagaaag aggctcgtgc ctggcaggat cgcattaatg aactggaggg ggctcttagc
     3421 catgagaaag atttaagccg taggttgctg gcagagaaag agcgcgagat tgctgagatc
     3481 agagccaaga tgcagcagca gctggatgag tatgagcaac tgctggacgt caaactagcg
     3541 ctggacatgg agatcaatgc ttacagaaag cttctggagg gagaagaaga aaggttgaaa
     3601 ctgtctccaa gtccttcatc tcgtgtgact gtgtcacgag catcctccag tcgcagtgtt
     3661 cgtactaccc gtggcaagag aaagcgtgtg gatgtggagg agtcggaggc aagcagcagt
     3721 gtcagtatcg ctcattctgc atctgccact ggaaacgtct ccatcgatga gctggatgta
     3781 gatgggaagt tcatccgctt gcacaacaac tctgagcagg accagccgat ggcaggattt
     3841 cagctgacca ggactattgg agaagttact gcaacatata aattcactgc aaaatacaat
     3901 ctgaaggctg gacagaaagt gacgatctgg gcatctaatg caggcataag ctccaaccct
     3961 cctgctgacc tgatatggaa gaaccagcct tcatggggga ctggggagaa cataaaagtg
     4021 gctcttttga gcccagcagg agaggaagta gctgtgagaa gcacagtatt caagactgca
     4081 gtggaagatg aagacgacga tgaggaggaa gaaaaggaag ctgaagtcat tgaggagcat
     4141 ttctatcacc agcagggaga tccacgcgct gctaagagag gatgtttgat catgtaatct
     4201 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     4261 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     4321 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     4381 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     4441 gatgcggtgg gctctatgga ccggactgag gcggaaagaa ccagctgtgg aatgtgtgtc
     4501 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4561 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4621 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     4681 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     4741 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     4801 ttggaggcct aggcttttgc aaaaagctag attttctgac cctagccacc atgaccgagt
     4861 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg
     4921 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg
     4981 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     5041 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     5101 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     5161 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     5221 tcctggccac cgtcggcgtg tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     5281 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     5341 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     5401 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga ggatctcgag
     5461 ctcgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     5521 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     5581 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
     5641 aagtaaaacc tctacaaatg tggtacttaa gagggatcga taaggatcta gcttgtggaa
     5701 ggctactcga aatgtttgac ccaagttaaa caatttaaag gcaatgctac caaatactaa
     5761 ttgagtgtat gtaaacttct gacccactgg gaatgtgatg aaagaaataa aagctgaaat
     5821 gaatcattct ctctactatt attctgatat ttcacattct taaaataaag tggtgatcct
     5881 aactgaccta agacagggaa tttttactag gattaaatgt caggaattgt gaaaaagtga
     5941 gtttaaatgt atttggctaa ggtgtatgta aacttccgac ttcaactgta tagggatcct
     6001 ctagctagag tcgacctcga gggggggccc ggtacccagc ttttgttccc tttagtgagg
     6061 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     6121 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     6181 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     6241 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     6301 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     6361 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     6421 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     6481 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     6541 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     6601 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     6661 ttcgggaacc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     6721 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     6781 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     6841 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     6901 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     6961 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7021 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7081 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7141 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7201 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7261 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7321 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7381 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7441 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     7501 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     7561 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     7621 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7681 cggtcctccg atcgttgtca gaagtaactt ggccgcagtg ttatcactca tggttatggc
     7741 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     7801 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     7861 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     7921 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7981 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8041 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8101 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     8161 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     8221 tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggagtggt
     8281 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     8341 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     8401 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     8461 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     8521 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     8581 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     8641 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttccat
     8701 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     8761 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     8821 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag
     8881 ggcgaattgg agctcggatc cctatacagt tgaagtcgga agtttacata cacttaagtt
     8941 ggagtcatta aaactcgttt ttcaactact ccacaaattt cttgttaaca aacaatagtt
     9001 ttggcaagtc agttaggaca tctactttgt gcatgacaca agtcattttt ccaacaattg
     9061 tttacagaca gattatttca cttataattc actgtatcac aattccagtg ggtcagaagt
     9121 ttacatacac taagttgact gtgcctttaa acagcttgga aaattccaga aaatgatgtc
     9181 atggctttag
//