LOCUS       Exported                9444 bp ds-DNA     circular SYN 11-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9444)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Dec 11, 2025 from SnapGene 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9444
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          2101..2415
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    14..566
                     /label=CMV_immearly_promoter
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     481..500
                     /label=NLS-F
     misc_feature    523..543
                     /label=cmv_f
     misc_feature    593
                     /label=P1
     protein_bind    595..619
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    595..602
                     /label=attR1
     misc_feature    595..602
                     /label=attR1+teminus
     primer_bind     complement(601..622)
                     /label=plx304-F
     primer_bind     complement(603..622)
                     /label=V-R
     misc_feature    603..619
                     /label=attB1
     primer_bind     complement(615..630)
                     /label=Primer 1
     misc_feature    619..624
                     /label=BstBI
     misc_feature    625..633
                     /label=Kozak
     misc_feature    634..675
                     /label=V5 Tag
     misc_feature    676..681
                     /label=AgeI
     misc_feature    682..2076
                     /label=G3BP1
     misc_feature    2077..2094
                     /label=GS linker
     primer_bind     complement(2081..2100)
                     /label=R_GS_pcDNA3.1
     primer_bind     2095..2131
                     /label=314F_S3G_04/29
     misc_feature    2095..2100
                     /label=BamH1
     misc_feature    2101..2415
                     /label=Lantern
     misc_feature    2107..2109
                     /label=S3G
     primer_bind     complement(2305..2363)
                     /label=315R_W80N_04/25
     misc_feature    2338..2340
                     /label=W80N
     primer_bind     2341..2367
                     /label=316F_04/25
     primer_bind     complement(2384..2416)
                     /label=316R_Q102A_04/25
     misc_feature    2404..2406
                     /label=Q102A
     primer_bind     2419..2433
                     /label=Primer 2
     primer_bind     2419..2433
                     /label=V-F
     misc_feature    2419..2424
                     /label=Nhe1
     primer_bind     2420..2436
                     /label=plx304-R
     misc_feature    2450
                     /label=p_overlap
                     /label=p_overlap(1)
     misc_feature    2451..3039
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2541..2560)
                     /label=NLS-R
     CDS             complement(2922..2933)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3111..3344
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    3116..3140
                     /label=Sequencing primer_fwd_3059
     misc_feature    3164..3344
                     /label=HIV1 3' LTR
     polyA_signal    3422..3543
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      3583..3718
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3738..3758)
                     /label=T7
     promoter        complement(3739..3757)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3767..3784)
                     /label=M13-fwd
     primer_bind     complement(3767..3783)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3855..3923
                     /label=LacZ alpha
     rep_origin      3925..4380
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      3942..4248
                     /label=F1 ori
     promoter        4406..4510
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4511..5371
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             4709..5368
                     /label=AmpR
     rep_origin      5466..6148
                     /label=ColE1 origin
     rep_origin      5542..6130
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6418..6439
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6454..6484
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     misc_binding    6488..6510
                     /label=LacO
     protein_bind    6492..6508
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6516..6536
                     /label=M13-rev
     primer_bind     6516..6532
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6553..6571
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     6554..6573
                     /label=T3
     promoter        6597..6825
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    6777..6802
                     /label=Fwd sequencing primer_6720
     LTR             6826..7006
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6826..7006
                     /label=HIV1 5'LTR
     misc_feature    7053..7178
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7117..7161
                     /label=Psi Packaging Signal
     misc_feature    7671..7904
                     /label=RRE
     misc_feature    7928..7950
                     /label=Sequencing primer_Rev_7893
     promoter        8308..8808
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             8820..9215
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9275..9392
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttgtacaaaa aagcaggctt cgaagccacc atgggcaagc ccatccccaa ccccctgctg
      661 ggcctggaca gcaccaccgg tgtgatggag aagcctagtc ccctgctggt cgggcgggaa
      721 tttgtgagac agtattacac actgctgaac caggccccag acatgctgca tagattttat
      781 ggaaagaact cttcttatgt ccatggggga ttggattcaa atggaaagcc agcagatgca
      841 gtctacggac agaaagaaat ccacaggaaa gtgatgtcac aaaacttcac caactgccac
      901 accaagattc gccatgttga tgctcatgcc acgctaaatg atggtgtggt agtccaggtg
      961 atggggcttc tctctaacaa caaccaggct ttgaggagat tcatgcaaac gtttgtcctt
     1021 gctcctgagg ggtctgttgc aaataaattc tatgttcaca atgatatctt cagataccaa
     1081 gatgaggtct ttggtgggtt tgtcactgag cctcaggagg agtctgaaga agaagtagag
     1141 gaacctgaag aaagacagca aacacctgag gtggtacctg atgattctgg aactttctat
     1201 gatcaggcag ttgtcagtaa tgacatggaa gaacatttag aggagcctgt tgctgaacca
     1261 gagcctgatc ctgaaccaga accagaacaa gaacctgtat ctgaaatcca agaggaaaag
     1321 cctgagccag tattagaaga aactgcccct gaggatgctc agaagagttc ttctccagca
     1381 cctgcagaca tagctcagac agtacaggaa gacttgagga cattttcttg ggcatctgtg
     1441 accagtaaga atcttccacc cagtggagct gttccagtta ctgggatacc acctcatgtt
     1501 gttaaagtac cagcttcaca gccccgtcca gagtctaagc ctgaatctca gattccacca
     1561 caaagacctc agcgggatca aagagtgcga gaacaacgaa taaatattcc tccccaaagg
     1621 ggacccagac caatccgtga ggctggtgag caaggtgaca ttgaaccccg aagaatggtg
     1681 agacaccctg acagtcacca actcttcatt ggcaacctgc ctcatgaagt ggacaaatca
     1741 gagcttaaag atttctttca aagttatgga aacgtggtgg agttgcgcat taacagtggt
     1801 gggaaattac ccaattttgg ttttgttgtg tttgatgatt ctgagcctgt tcagaaagtc
     1861 cttagcaaca ggcccatcat gttcagaggt gaggtccgtc tgaatgtcga agagaagaag
     1921 actcgagctg ccagggaagg cgaccgacga gataatcgcc ttcggggacc tggaggccct
     1981 cgaggtgggc tgggtggtgg aatgagaggc cctccccgtg gaggcatggt gcagaaacca
     2041 ggatttggag tgggaagggg gcttgcgcca cggcagggag gttcaggagg cagcggatcc
     2101 gaaaagggct ttgtgattac cgatccgcgc ctgccagaca acccgatcat tttcgcgagc
     2161 gatggctttc tggagttaac cgaatattct cgtgaggaaa ttctgggtcg caatggccgt
     2221 ttcttgcagg gtccggaaac ggatcaagcc accgtgcaga aaatccgcga tgcgattcgt
     2281 gaccaacgcg aaatcaccgt tcagctgatt aactatacga aaagcggcaa gaaatttaat
     2341 aacttactgc atctgcaacc gatgcgcgat cagaaaggcg aattgcaata tttcattggt
     2401 gtggccctgg atggctgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc
     2461 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     2521 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     2581 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     2641 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     2701 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     2761 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     2821 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     2881 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     2941 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     3001 ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac
     3061 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     3121 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     3181 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     3241 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     3301 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt atagtagttc
     3361 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     3421 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     3481 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     3541 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     3601 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     3661 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     3721 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     3781 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     3841 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     3901 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     3961 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     4021 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     4081 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     4141 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     4201 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     4261 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     4321 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     4381 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     4441 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     4501 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     4561 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     4621 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     4681 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     4741 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     4801 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     4861 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     4921 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     4981 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     5041 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     5101 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     5161 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     5221 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     5281 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     5341 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     5401 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     5461 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     5521 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     5581 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     5641 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     5701 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     5761 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     5821 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     5881 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     5941 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6001 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6061 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6121 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6181 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     6241 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     6301 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     6361 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     6421 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     6481 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     6541 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     6601 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     6661 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     6721 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     6781 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     6841 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     6901 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     6961 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     7021 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     7081 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     7141 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     7201 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     7261 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     7321 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     7381 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     7441 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     7501 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     7561 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     7621 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     7681 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     7741 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     7801 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     7861 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     7921 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     7981 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     8041 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     8101 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     8161 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     8221 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     8281 acccacctcc caaccccgag gggacccttg cgccttttcc aaggcagccc tgggtttgcg
     8341 cagggacgcg gctgctctgg gcgtggttcc gggaaacgca gcggcgccga ccctgggtct
     8401 cgcacattct tcacgtccgt tcgcagcgtc acccggatct tcgccgctac ccttgtgggc
     8461 cccccggcga cgcttcctgc tccgccccta agtcgggaag gttccttgcg gttcgcggcg
     8521 tgccggacgt gacaaacgga agccgcacgt ctcactagta ccctcgcaga cggacagcgc
     8581 cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca
     8641 gggcgcgccg agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg tggggcggta
     8701 gtgtgggccc tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg
     8761 tcggcagtcg gctccctcgt tgaccgaatc accgacctct ctccccaggg ggtaccacca
     8821 tggccaagcc tttgtctcaa gaagaatcca ccctcattga aagagcaacg gctacaatca
     8881 acagcatccc catctctgaa gactacagcg tcgccagcgc agctctctct agcgacggcc
     8941 gcatcttcac tggtgtcaat gtatatcatt ttactggggg accttgtgca gaactcgtgg
     9001 tgctgggcac tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa
     9061 atgagaacag gggcatcttg agcccctgcg gacggtgccg acaggtgctt ctcgatctgc
     9121 atcctgggat caaagccata gtgaaggaca gtgatggaca gccgacggca gttgggattc
     9181 gtgaattgct gccctctggt tatgtgtggg agggcctgca gctgcagtag taagaattct
     9241 agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     9301 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     9361 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     9421 cactttggcg ccggctcgag gggg
//
