LOCUS       DTR30_Sap1-TV-IRES-Neon 6586 bp ds-DNA     circular     04-SEP-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          66..84
                     /label="BP253 Seq TetOn (T2) 500 Fw"
                     /note="sequence: ctatgcccccacttctgaaa"
     primer          complement(163..184)
                     /label="YB104_TVBB_Seq_REV1"
                     /note="sequence: gcggataaagttgcaggaccac"
     primer          289..310
                     /label="YB105_TVBB_Seq_FWD1"
                     /note="sequence: gctcgtcgtttggtatggcttc"
     primer          complement(676..697)
                     /label="YB106_TVBB_Seq_REV2"
                     /note="sequence: gcacgagtgggttacatcgaac"
     Signal peptide  complement(746..814)
                     /label="AmpR signal peptide"
     primer          787..808
                     /label="YB107_TVBB_Seq_FWD2"
                     /note="sequence: gggcgacacggaaatgttgaat"
     Promoter        complement(815..919)
                     /label="AmpR promoter"
     primer          900..939
                     /label="Pshor55"
                     /note="sequence: AAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGC"
     primer          903..922
                     /label="YB115_TVUHA_Seq"
                     /note="sequence: caaataggggttccgcgcac"
     primer          complement(903..942)
                     /label="Pshor53"
                     /note="sequence: GTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTG"
     primer          complement(966..990)
                     /label="YB94_AAVTV_BB_Rev"
                     /note="sequence: gcggtattttctccttacgcatctg"
     primer          complement(966..1043)
                     /label="JH124_R_addSap_inf-ires"
                     /note="sequence: CCCAATACCACATCAGCCTGAAGAGCCTTTAAACGCGATCGCGCTCTTCCGTAGcggtattttctccttacgcatctg"
     misc_feature    971..990
                     /label="In-Fus overhang"
     misc_feature    1026..1028
                     /label="This will be added as an extra Gly to the targeted gene right before the stopcodon"
     misc_feature    1029..1031
                     /label="stopcodon"
     primer          1029..1047
                     /label="JH123_F_IRES"
                     /note="sequence: TGATGTGGTATTGGGGGCC"
     misc_feature    1041..1142
                     /label="'spacer'"
     primer          1112..1128
                     /label="MH32_Fw_IRES into Notch1"
                     /note="sequence: GTcGCTAGCtagCCCGGGAATTCCGCC"
     misc_feature    1143..1695
                     /label="IRES"
     primer          complement(1236..1252)
                     /label="BP572 Rv Prepare TV27"
                     /note="sequence: AGGGCCAGGTTTCCGgg"
     primer          1266..1285
                     /label="BP127 IRES Seq 160 Fw"
                     /note="sequence: GCATTCCTAGGGGTCTTTCC"
     primer          complement(1266..1285)
                     /label="BP128 IRES Seq 160 Rev"
                     /note="sequence: GGAAAGACCCCTAGGAATGC"
     primer          1363..1384
                     /label="K4-CMV-Neon (ires-Blast) 8333-8354 FWD 18"
                     /note="sequence: AACGTCTGTAGCGACCCTTTGC"
     primer          1364..1385
                     /label="K4-CMV-Neon (ires-Blast) 8334-8355 FWD 19"
                     /note="sequence: ACGTCTGTAGCGACCCTTTGCA"
     primer          1365..1386
                     /label="K4-CMV-Neon (ires-Blast) 8335-8356 FWD 16"
                     /note="sequence: CGTCTGTAGCGACCCTTTGCAG"
     primer          1365..1386
                     /label="K4-CMV-Neon (ires-Blast) 8335-8356 FWD 20"
                     /note="sequence: CGTCTGTAGCGACCCTTTGCAG"
     primer          1366..1387
                     /label="K4-CMV-Neon (ires-Blast) 8336-8357 FWD 17"
                     /note="sequence: GTCTGTAGCGACCCTTTGCAGG"
     primer          complement(1426..1451)
                     /label="MH144_Rv nls-IRES into TV35B"
                     /note="sequence: GTGTATCTTATACACGTGGCTTTTGG"
     primer          complement(1443..1464)
                     /label="K4-CMV-Neon (ires-Blast) 8413-8434 REV 7"
                     /note="sequence: GCCGCCTTTGCAGGTGTATCTT"
     primer          complement(1443..1464)
                     /label="K4-CMV-Neon (ires-Blast) 8413-8434 REV 6"
                     /note="sequence: GCCGCCTTTGCAGGTGTATCTT"
     primer          complement(1444..1465)
                     /label="K4-CMV-Neon (ires-Blast) 8414-8435 REV 10"
                     /note="sequence: TGCCGCCTTTGCAGGTGTATCT"
     primer          complement(1445..1466)
                     /label="K4-CMV-Neon (ires-Blast) 8415-8436 REV 9"
                     /note="sequence: GTGCCGCCTTTGCAGGTGTATC"
     primer          complement(1445..1466)
                     /label="K4-CMV-Neon (ires-Blast) 8415-8436 REV 8"
                     /note="sequence: GTGCCGCCTTTGCAGGTGTATC"
     primer          1594..1613
                     /label="BP129 IRES Seq 490 Fw"
                     /note="sequence: TCGGTGCACATGCTTTACAT"
     primer          complement(1706..1739)
                     /label="JH113_R_IRES_toDTR4"
                     /note="sequence: ctcgcccttgctcacCATGGTGGCGACCGTTGTG"
     primer          1714..1739
                     /label="BP464 Scarlet-I into K9 PCR2 Fw"
                     /note="sequence: tcgccaccATGGTGAGCAAGGGCGAG"
     misc_feature    1716..1725
                     /label="Kozak"
     primer          complement(1720..1739)
                     /label="AP05_R_mNeon_out_mScarlet_in"
                     /note="sequence: ctcgcccttgctcacCATGG"
     CDS             1722..1724
                     /translation="M"
     primer          1725..1737
                     /label="JH50_FL12_F_Neon"
                     /note="sequence: AGAGGATCCaccATGgtgagcaagggcg"
     primer          1725..1742
                     /label="JH110_F_mNG_RemoveP2A"
                     /note="sequence: GTGAGCAAGGGCGAGGAG"
     Fluorescent ... 1725..2429
                     /label="mNeonGreen"
     CDS             1725..2429
                     /label="Translation 1725-2429"
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDGASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIISTFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQKAFTDVMGMDELYK"
     primer          complement(1727..1748)
                     /label="JP063"
                     /note="sequence: gttatcctcctcgcccttgctc"
     primer          complement(1744..1765)
                     /label="YB116_TVBB_Seq"
                     /note="sequence: gctgggagagaggccatgttat"
     primer          2021..2042
                     /label="YB117_TVBB_Seq"
                     /note="sequence: gcagtttgaagatggtgcctcc"
     primer          2344..2365
                     /label="K4-CMV-Neon (ires-Blast) 7945-7966 FWD 15"
                     /note="sequence: agctcaagcactccaagaccga"
     primer          2347..2368
                     /label="K4-CMV-Neon (ires-Blast) 7948-7969 FWD 14"
                     /note="sequence: tcaagcactccaagaccgagct"
     primer          2349..2370
                     /label="K4-CMV-Neon (ires-Blast) 7950-7971 FWD 13"
                     /note="sequence: aagcactccaagaccgagctca"
     primer          2350..2371
                     /label="K4-CMV-Neon (ires-Blast) 7951-7972 FWD 12"
                     /note="sequence: agcactccaagaccgagctcaa"
     primer          2409..2429
                     /label="JB_0053_fwinf"
                     /note="sequence: ggcatggacgagctgtacaag"
     primer          complement(2409..2429)
                     /label="JB_0052_rvinf"
                     /note="sequence: cttgtacagctcgtccatgcc"
     primer          2430..2449
                     /label="JH37_F_DTR5_RemoveNeon"
                     /note="sequence: ATGGCCCCAAAGAAGAAGCG"
     misc_feature    2430..2480
                     /label="NLS"
     CDS             2430..2480
                     /label="Translation 2430-2480"
                     /translation="MAPKKKRKVGIHGVPAA"
     primer          2436..2459
                     /label="YB59_SV40NLS_Fwd_Seq"
                     /note="sequence: CCAAAGAAGAAGCGGAAGGTCGGT"
     primer          2436..2459
                     /label="YB59_SV40NLS_Fwd_Seq"
                     /note="sequence: CCAAAGAAGAAGCGGAAGGTCGGT"
     primer          complement(2436..2459)
                     /label="YB58_SV40NLS_Rev_Seq"
                     /note="sequence: ACCGACCTTCCGCTTCTTCTTTGG"
     primer          complement(2436..2459)
                     /label="YB58_SV40NLS_Rev_Seq"
                     /note="sequence: ACCGACCTTCCGCTTCTTCTTTGG"
     primer          complement(2463..2480)
                     /label="JH57_R_RemoveDTR"
                     /note="sequence: GGCTGCTGGGACTCCGTG"
     primer          complement(2468..2483)
                     /label="JH51_FL13_R_Neon"
                     /note="sequence: GCACAGGAATTCTTAGGCTGCTGGGACT"
     misc_feature    2481..2489
                     /label="P2A linker"
     primer          2481..2503
                     /label="JB_0002_fw"
                     /note="sequence: GGAAGCGGAGCGACGAATTTTAG"
     Ribosome ski... 2481..2546
                     /label="Optimized P2A (Chiu-An Lo et al.)"
     CDS             2481..2546
                     /label="Translation 2481-2546"
                     /translation="GSGATNFSLLKQAGDVEENPGP"
     primer          2503..2526
                     /label="YB175_KRASGT_DHA_F"
                     /note="sequence: GTCTACTGAAACAAGCGGGAGACG"
     primer          complement(2503..2526)
                     /label="YB178_KRASGT_UHA_R"
                     /note="sequence: CGTCTCCCGCTTGTTTCAGTAGAC"
     primer          complement(2505..2523)
                     /label="JH99_P2A_R_rspo3"
                     /note="sequence: CTCCCGCTTGTTTCAGTAG"
     primer          2526..2546
                     /label="YB111_TVBB_Seq_FWD4"
                     /note="sequence: GTGGAGGAAAACCCTGGACCT"
     primer          complement(2526..2546)
                     /label="JH43_FL4_R_P2A"
                     /note="sequence: AGGTCCAGGGTTTTCCTCCAC"
     primer          2547..2565
                     /label="JH79_DTR_F"
                     /note="sequence: ATGAAGCTGCTGCCGTCGG"
     misc_feature    2547..3173
                     /label="wt DTR"
     CDS             2547..3173
                     /label="Translation 2547-3173"
                     /translation="MKLLPSVVLKLFLAAVLSALVTGESLERLRRGLAAGTSNPDPPTVSTDQLLPLGGGRDRKVRDLQEADLDLLRVTLSSKPQALATPNKEEHGKRKKKGKGLGKKRDPCLRKYKDFCIHGECKYVKELRAPSCICHPGYHGERCHGLSLPVENRLYTYDHTTILAVVAVVLSSVCLLVIVGLLMFRYHRRGGYDVENEEKVKLGMTNSH*"
     primer          2679..2697
                     /label="JH27_DTR_F_kpn1-pvu2"
                     /note="sequence: GTATCCACGGACCAGCTGC"
     primer          2875..2917
                     /label="JH25_DTR_V117I_F"
                     /note="sequence: GGAAATACAAGGACTTCTGCATCCATGGAGAATGCAAATATGT"
     primer          complement(2875..2917)
                     /label="JH26_DTR_V117I_R"
                     /note="sequence: ACATATTTGCATTCTCCATGGATGCAGAAGTCCTTGTATTTCC"
     misc_feature    2895..2897
                     /label="I117"
     primer          2971..3005
                     /label="JH23_DTR_V148L_F"
                     /note="sequence: GGTGTCATGGGCTGAGCCTCCCAGTGGAAAATCGC"
     primer          complement(2971..3005)
                     /label="JH24_DTR_V148L_R"
                     /note="sequence: GCGATTTTCCACTGGGAGGCTCAGCCCATGACACC"
     misc_feature    2988..2990
                     /label="L148"
     primer          3090..3115
                     /label="JH32_F_DTR"
                     /note="sequence: CTCATGTTTAGGTACCATAGGAGAGG"
     primer          complement(3095..3119)
                     /label="JH28_DTR_R_kpn1-puv2"
                     /note="sequence: ACCTCCTCTCCTATGGTACCTAAAC"
     primer          complement(3144..3173)
                     /label="JH31_R_DTR"
                     /note="sequence: TCAGTGGGAATTAGTCATGCCCAACTTCAC"
     primer          complement(3149..3170)
                     /label="JH83_R_DTR"
                     /note="sequence: GTGGGAATTAGTCATGCCCAAC"
     primer          complement(3154..3173)
                     /label="JH16_R_TVLgr5dtrneon_PCR"
                     /note="sequence: TCAGTGGGAATTAGTCATGC"
     primer          3159..3197
                     /label="JH115_F_WPRE_toDTR4"
                     /note="sequence: ACTAATTCCCACTGAGCTCGAAGATGGTGACTGAATTCG"
     primer          3166..3197
                     /label="JH120_F_RemoveDTR_DTR24"
                     /note="sequence: GGAGTCCCAGCAGCCTGAGCTCGAAGATGGTGACTGAATTCG"
     primer          3171..3197
                     /label="AP04_F"
                     /note="sequence: TGAgctcgaagatggtgactgaattcg"
     primer          3174..3197
                     /label="JH117_F_RemoveDTR_DTR24"
                     /note="sequence: GCTCGAAGATGGTGACTGAATTCG"
     misc_feature    3215..3803
                     /label="WPRE"
     primer          3624..3641
                     /label="Pshor50"
                     /note="sequence: cttggctgctcgcctgtg"
     CDS             complement(3686..3697)
                     /label="Factor Xa site"
     primer          complement(3796..3829)
                     /label="JH116_R_WPRE_toDTR4"
                     /note="sequence: AAGGCACAGTCGAGGTCGGTATCGATGCGGGGAG"
     primer          3800..3836
                     /label="JH29_F_bGHpA_intoDTR3"
                     /note="sequence: ACTAATTCCCACTGACCTCGACTGTGCCTTCTAGTTG"
     primer          3807..3836
                     /label="JH56_F_RemoveDTR"
                     /note="sequence: GGAGTCCCAGCAGCCTGACCTCGACTGTGCCTTCTAGTTG"
     primer          3812..3830
                     /label="JH82_F_stopPolyA"
                     /note="sequence: TGACCTCGACTGTGCCTTC"
     primer          3815..3833
                     /label="JH114_F_DTR4_PolyA"
                     /note="sequence: CCTCGACTGTGCCTTCTAG"
     misc_feature    3815..4045
                     /label="Joris bGH PolyA"
     polyA_signal    3821..4045
                     /label="bGH poly(A) signal"
     Poly-A site     3830..4045
                     /label="bGH_PA_terminator"
     primer          complement(3929..3947)
                     /label="JP009"
                     /note="sequence: CTACTCAGACAATGCGATG"
     primer          complement(3968..3987)
                     /label="JP032"
                     /note="sequence: GCTGTCCTGCCCCACCCCAC"
     primer          complement(4027..4045)
                     /label="JH94_R_pA_removeLoxP-puro"
                     /note="sequence: CCATAGAGCCCACCGCATC"
     primer          complement(4027..4060)
                     /label="JH30_R_bGHpA_intoDTR3"
                     /note="sequence: GCTATACGAAGTTATCCATAGAGCCCACCGCATC"
     primer          4031..4066
                     /label="JH21_F_addInfTvToLoxp"
                     /note="sequence: ACTAATTCCCACTGAATAACTTCGTATAGCATACAT"
     primer          4031..4097
                     /label="JH19_F_addInfLoxpToPuroSc10"
                     /note="sequence: ACTAATTCCCACTGAATAACTTCGTATAGCATACATTATACGAAGTTATTAATTCGCGGCCGCACCG"
     misc_feature    4046..4079
                     /label="LoxP"
     primer          4046..4097
                     /label="JH17_F_addLoxPtoPuroSc10"
                     /note="sequence: ATAACTTCGTATAGCATACATTATACGAAGTTATTAATTCGCGGCCGCACCG"
     primer          complement(4088..4102)
                     /label="BP365 Make F22 Rev"
                     /note="sequence: CTACCCGGTgcggcc"
     misc_feature    4097..4596
                     /label="PGK promoter"
     primer          complement(4136..4152)
                     /label="BP406 Turq2 van F7 into miniTol-Y-ER-C_Rv"
                     /note="sequence: CGGGGCTGCTAAAGCGC"
     primer          complement(4205..4222)
                     /label="KO rv-(pLV)_PGK-seq.primer #21"
                     /note="sequence: GTTGGCGCCTACCGGTGG"
     primer          4249..4267
                     /label="BP119 PGK Seq Fw170"
                     /note="sequence: GCGCCACCTTCTACTCCTC"
     primer          4249..4267
                     /label="BP119 PGK Seq Fw170"
                     /note="sequence: GCGCCACCTTCTACTCCTC"
     primer          4375..4392
                     /label="JP005"
                     /note="sequence: CAATGGAAGCGGGTAGGC"
     primer          complement(4529..4548)
                     /label="BP120 PGK Seq Rev 430"
                     /note="sequence: CTTTTGAAGCGTGCAGAATG"
     primer          complement(4529..4548)
                     /label="BP120 PGK Seq Rev 430"
                     /note="sequence: CTTTTGAAGCGTGCAGAATG"
     primer          complement(4596..4613)
                     /label="KO rv-pCS2_SV40-seq.primer #18"
                     /note="sequence: GTCAACAGGCTGCAGGTC"
     promoter        4608..4655
                     /label="EM7 promoter"
     primer          complement(4653..4673)
                     /label="JH45_FL7_R_PGK"
                     /note="sequence: GGTTTAGTTCCTCACCTTGTC"
     primer          4668..4715
                     /label="BP040 Puro Fw"
                     /note="sequence: TAAACCGTCGACGATtTCACTAGTCTCGAAGCCACCATGACCGAGTAC"
     primer          complement(4680..4703)
                     /label="JH72_PGK_R"
                     /note="sequence: GGTGGCTTCGAGACTAGTGAAATC"
     primer          4698..4723
                     /label="BP095 Fusion PCR PGK-Puro 03 Fw"
                     /note="sequence: GCCACCATGACCGAGTACAAGCCCAC"
     primer          4704..4723
                     /label="JH71_PuroR_F"
                     /note="sequence: ATGACCGAGTACAAGCCCAC"
     CDS             4704..5303
                     /label="PuroR"
     primer          complement(4833..4851)
                     /label="JP030"
                     /note="sequence: GCAGCTCGGTGACCCGCTC"
     primer          complement(4839..4858)
                     /label="BP123 Puro Seq Rev140"
                     /note="sequence: AGTTCTTGCAGCTCGGTGAC"
     primer          complement(4839..4858)
                     /label="BP123 Puro Seq Rev140"
                     /note="sequence: AGTTCTTGCAGCTCGGTGAC"
     primer          4841..4859
                     /label="KO fw-Puro-seq.primer #37"
                     /note="sequence: CACCGAGCTGCAAGAACTC"
     primer          5103..5120
                     /label="BP121 Puro Seq Fw400"
                     /note="sequence: CACCAGGGCAAGGGTCTG"
     primer          5103..5120
                     /label="BP121 Puro Seq Fw400"
                     /note="sequence: CACCAGGGCAAGGGTCTG"
     primer          complement(5103..5120)
                     /label="BP124 Puro Seq Rev410"
                     /note="sequence: CAGACCCTTGCCCTGGTG"
     primer          complement(5103..5120)
                     /label="BP124 Puro Seq Rev410"
                     /note="sequence: CAGACCCTTGCCCTGGTG"
     primer          5200..5219
                     /label="BP122 Puro Seq Fw510"
                     /note="sequence: GCAACCTCCCCTTCTACGAG"
     primer          5200..5219
                     /label="BP122 Puro Seq Fw510"
                     /note="sequence: GCAACCTCCCCTTCTACGAG"
     primer          5304..5332
                     /label="JH44_FL6_F_Puro"
                     /note="sequence: CTAGAACTATAGTGAGTCGTATTACGTAG"
     promoter        complement(5310..5327)
                     /label="T7 promoter"
     polyA_signal    5332..5466
                     /label="SV40 poly(A) signal"
     primer          5409..5432
                     /label="BP190 Seq Rev into TagGFP of Casper3"
                     /note="sequence: TGAAATTTGTGATGCTATTGCTTT"
     primer          5508..5527
                     /label="BP067 Seq primer polyA Fw"
                     /note="sequence: ggaggtgtgggaggtttttt"
     primer          complement(5508..5527)
                     /label="BP066 Seq primer polyA Rev"
                     /note="sequence: aaaaaacctcccacacctcc"
     primer          complement(5508..5569)
                     /label="JH18_R_addLoxPtoPuroSc10"
                     /note="sequence: ATAACTTCGTATAATGTATGCTATACGAAGTTATGCTTAATTAAAAAACCTCCCACACCTCC"
     primer          complement(5520..5569)
                     /label="JH122_R_LOXP"
                     /note="sequence: ATAACTTCGTATAATGTATGCTATACGAAGTTATGCTTAATTAAAAAACC"
     primer          5534..5556
                     /label="JH21_F_addInfTvToLoxp"
                     /note="sequence: ACTAATTCCCACTGAATAACTTCGTATAGCATACAT"
     misc_feature    5536..5569
                     /label="LoxP"
     primer          5555..5626
                     /label="JH33_F_AddSap1toDTR4"
                     /note="sequence: ATTATACGAAGTTATTGACGAAGAGCGTTTAAACGCGAACCGGCTCTTCGAATTcctcgctcactgactcgc"
     primer          5608..5626
                     /label="YB101_AAVTV_BB_Fwd"
                     /note="sequence: tcctcgctcactgactcgc"
     misc_feature    5608..5627
                     /label="In-Fus overhang"
     primer          5659..5680
                     /label="YB113_TVBB_Seq_FWD5"
                     /note="sequence: tcagctcactcaaaggcggtaa"
     primer          complement(5659..5680)
                     /label="YB114_TVDHA_Seq"
                     /note="sequence: ttaccgcctttgagtgagctga"
     rep_origin      complement(5763..6445)
                     /label="ColE1 origin"
     primer          complement(5813..5830)
                     /label="MH189_Fw ori 2"
                     /note="sequence: TTGAGCGTCGATTTTTGT"
     Kozak sequence  complement(5837..5843)
                     /label="Kozak"
     primer          complement(6103..6124)
                     /label="JH83_R_DTR"
                     /note="sequence: GTGGGAATTAGTCATGCCCAAC"
     primer          6288..6303
                     /label="MH188_Rv ori 1"
                     /note="sequence: ATCCGGCAAACAAACC"
     primer          complement(6349..6368)
                     /label="MH187_Fw ori 1"
                     /note="sequence: TGAGATCCTTTTTTTCTGCG"
     primer          6547..6563
                     /label="MH193_Rv Amp 2"
                     /note="sequence: TGCTTAATCAGTGAGGC"
     primer          complement(6555..6574)
                     /label="BP 048 Amp_HA2_control_fw"
                     /note="sequence: CTGAGATAGGTGCCTCACTG"
ORIGIN
        1 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
       61 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
      121 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
      181 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
      241 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
      301 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
      361 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
      421 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
      481 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
      541 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
      601 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
      661 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
      721 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
      781 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
      841 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
      901 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgatgcg gtgtgaaata
      961 ccgcacagat gcgtaaggag aaaataccgc TACGGAAGAG CGCGATCGCG TTTAAAGGCT
     1021 CTTCAGGCTG ATGTGGTATT GGGGGCCAAG TCTGTACAGC ATCTTGAGTC CCTTTTTACC
     1081 GCTGTTACCA ATTTTCTTTT GTCTTTGGGT AAACCCGGGA ATTCCGCCCC TCTCCCCCCC
     1141 TAACGTTACT GGCCGAAGCC GCTTGGAATA AGGCCGGTGT GCGTTTGTCT ATATGTTATT
     1201 TTCCACCATA TTGCCGTCTT TTGGCAATGT GAGGGCCCGG AAACCTGGCC CTGTCTTCTT
     1261 GACGAGCATT CCTAGGGGTC TTTCCCCTCT CGCCAAAGGA ATGCAAGGTC TGTTGAATGT
     1321 CGTGAAGGAA GCAGTTCCTC TGGAAGCTTC TTGAAGACAA ACAACGTCTG TAGCGACCCT
     1381 TTGCAGGCAG CGGAACCCCC CACCTGGCGA CAGGTGCCTC TGCGGCCAAA AGCCACGTGT
     1441 ATAAGATACA CCTGCAAAGG CGGCACAACC CCAGTGCCAC GTTGTGAGTT GGATAGTTGT
     1501 GGAAAGAGTC AAATGGCTCT CCTCAAGCGT ATTCAACAAG GGGCTGAAGG ATGCCCAGAA
     1561 GGTACCCCAT TGTATGGGAT CTGATCTGGG GCCTCGGTGC ACATGCTTTA CATGTGTTTA
     1621 GTCGAGGTTA AAAAAACGTC TAGGCCCCCC GAACCACGGG GACGTGGTTT TCCTTTGAAA
     1681 AACACGATGA TAATATGGAT ATGGCCACAA CGGTCGCCAC CATGgtgagc aagggcgagg
     1741 aggataacat ggcctctctc ccagcgacac atgagttaca catctttggc TCTatcaacg
     1801 gtgtggactt tgacatggtg ggtcagGGAa ccGGAaatcc aaatgatggt tatgaggagt
     1861 taAATctgaa gTCAaccaag ggtgacctcc agttcTCTCC Ttggattctg gtccctcata
     1921 tcgggtatgg cttccatcag tacctgCCTt accctgacgg gatgTCTcct ttccaggccg
     1981 ccatggtaga tggcTCTgga taccaagtcc atAGAacaat gcagtttgaa gatggtgcct
     2041 cccttactgt tAATtacAGA tacACAtacg agggaagcCA Tatcaaagga gaggcccagg
     2101 tgaaggggac tggtttccct gctgacggtc ctgtgatgac caactcgctg accgctgcgg
     2161 actggtgcag gtcgaagAAA acttacccca acgacaaaac catcatcagt acctttaagt
     2221 ggagttacac cactggaaat ggcAAAAGAt accggagcac tgcgAGAacc ACTtacACTt
     2281 ttgccaagcc aatggcggct aactatctga agaaccagcc gatgtacgtg ttccgtaaga
     2341 cggagctcaa gcactccaag accgagctca acttcaagga gtggcaaaag gcctttaccg
     2401 atgtgatggg catggacgag ctgtacaagA TGGCCCCAAA GAAGAAGCGG AAGGTCGGTA
     2461 TCCACGGAGT CCCAGCAGCC GGAAGCGGAG CGACGAATTT TAGTCTACTG AAACAAGCGG
     2521 GAGACGTGGA GGAAAACCCT GGACCTATGA AGCTGCTGCC GTCGGTGGTG CTGAAGCTCT
     2581 TTCTGGCTGC AGTTCTCTCG GCACTGGTGA CTGGCGAGAG CCTGGAGCGG CTTCGGAGAG
     2641 GGCTAGCTGC TGGAACCAGC AACCCGGACC CTCCCACTGT ATCCACGGAC CAGCTGCTAC
     2701 CCCTAGGAGG CGGCCGGGAC CGGAAAGTCC GTGACTTGCA AGAGGCAGAT CTGGACCTTT
     2761 TGAGAGTCAC TTTATCCTCC AAGCCACAAG CACTGGCCAC ACCAAACAAG GAGGAGCACG
     2821 GGAAAAGAAA GAAGAAAGGC AAGGGGCTAG GGAAGAAGAG GGACCCATGT CTTCGGAAAT
     2881 ACAAGGACTT CTGCATCCAT GGAGAATGCA AATATGTGAA GGAGCTCCGG GCTCCCTCCT
     2941 GCATCTGCCA CCCGGGTTAC CATGGAGAGA GGTGTCATGG GCTGAGCCTC CCAGTGGAAA
     3001 ATCGCTTATA TACCTATGAC CACACAACCA TCCTGGCCGT GGTGGCTGTG GTGCTGTCAT
     3061 CTGTCTGTCT GCTGGTCATC GTGGGGCTTC TCATGTTTAG GTACCATAGG AGAGGAGGTT
     3121 ATGATGTGGA AAATGAAGAG AAAGTGAAGT TGGGCATGAC TAATTCCCAC TGAgctcgaa
     3181 gatggtgact gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     3241 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     3301 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     3361 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     3421 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     3481 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     3541 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     3601 gtcggggaaa tcatcgtcct ttccttggct gctcgcctat gttgccacct ggattctgcg
     3661 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     3721 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     3781 ctccctttgg gccgcctccc cgcatcgata ccgaCCTCGA CTGTGCCTTC TAGTTGCCAG
     3841 CCATCTGTTG TTTGCCCCTC CCCCGTGCCT TCCTTGACCC TGGAAGGTGC CACTCCCACT
     3901 GTCCTTTCCT AATAAAATGA GGAAATTGCA TCGCATTGTC TGAGTAGGTG TCATTCTATT
     3961 CTGGGGGGTG GGGTGGGGCA GGACAGCAAG GGGGAGGATT GGGAAGACAA TAGCAGGCAT
     4021 GCTGGGGATG CGGTGGGCTC TATGGATAAC TTCGTATAGC ATACATTATA CGAAGTTATT
     4081 AATTCGCGGC CGCACCGGGT AGGGGAGGCG CTTTTCCCAA GGCAGTCTGG AGCATGCGCT
     4141 TTAGCAGCCC CGCTGGGCAC TTGGCGCTAC ACAAGTGGCC TCTGGCCTCG CACACATTCC
     4201 ACATCCACCG GTAGGCGCCA ACCGGCTCCG TTCTTTGGTG GCCCCTTCGC GCCACCTTCT
     4261 ACTCCTCCCC TAGTCAGGAA GTTCCCCCCC GCCCCGCAGC TCGCGTCGTG CAGGACGTGA
     4321 CAAATGGAAG TAGCACGTCT CACTAGTCTC GTGCAGATGG ACAGCACCGC TGAGCAATGG
     4381 AAGCGGGTAG GCCTTTGGGG CAGCGGCCAA TAGCAGCTTT GCTCCTTCGC TTTCTGGGCT
     4441 CAGAGGCTGG GAAGGGGTGG GTCCGGGGGC GGGCTCAGGG GCGGGCTCAG GGGCGGGGCG
     4501 GGCGCCCGAA GGTCCTCCGG AGGCCCGGCA TTCTGCACGC TTCAAAAGCG CACGTCTGCC
     4561 GCGCTGTTCT CCTCTTCCTC ATCTCCGGGC CTTTCGACCT GCAGCCTGTT GACAATTAAT
     4621 CATCGGCATA GTATATCGGC ATAGTATAAT ACGACAAGGT GAGGAACTAA ACCGTCGACG
     4681 ATtTCACTAG TCTCGAAGCC ACCATGACCG AGTACAAGCC CACGGTGCGC CTCGCCACCC
     4741 GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC GTTCGCCGAC TACCCCGCCA
     4801 CGCGCCACAC CGTCGATCCG GACCGCCACA TCGAGCGGGT CACCGAGCTG CAAGAACTCT
     4861 TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT CGCGGACGAC GGCGCCGCGG
     4921 TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC GGTGTTCGCC GAGATCGGCC
     4981 CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA GCAACAGATG GAAGGCCTCC
     5041 TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC CACCGTCGGC GTCTCGCCCG
     5101 ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC CGGAGTGGAG GCGGCCGAGC
     5161 GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG CAACCTCCCC TTCTACGAGC
     5221 GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA AGGACCGCGC ACCTGGTGCA
     5281 TGACCCGCAA GCCCGGTGCC TGACTAGAac tatagtgagt cgtattacgt agatccagac
     5341 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     5401 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     5461 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     5521 gttttttaat taagcATAAC TTCGTATAGC ATACATTATA CGAAGTTATT GACGAAGAGC
     5581 GTTTAAACGC GAACCGGCTC TTCGAATtcc tcgctcactg actcgctgcg ctcggtcgtt
     5641 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     5701 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     5761 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     5821 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     5881 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     5941 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     6001 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     6061 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     6121 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     6181 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     6241 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     6301 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     6361 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     6421 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     6481 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     6541 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctat
//