LOCUS       Exported               11382 bp ds-DNA     circular SYN 01-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11382)
  AUTHORS   Friedman Lab
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jan 2, 2026 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11382
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             607..1497
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     misc_feature    1461..1497
                     /label=Primer
     misc_feature    1475..1489
                     /label=Overlap
     misc_feature    1479..1485
                     /label=Replace
     misc_feature    1499..1527
                     /label=MCS
                     /note="multiple cloning site of fluorescent protein 
                     plasmids"
     CDS             1534..8031
                     /codon_start=1
                     /product="myosin VIIa isoform 1"
                     /label=myosin VIIa isoform 1
                     /note="motor protein; alternatively spliced"
                     /protein_id="AAV87212.1"
                     /translation="MDLKSGQEFDVPIGAVVKLCDSGQIVVDDEDNEHWISPQNATHIK
                     PMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSP
                     EHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNNRDQCCIISGESGAGKTESTKLILQFL
                     AAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQ
                     YLLEKSRVCRQAPDERNYHVFYCMLEGMNEEEKKKLGLGQAADYNYLAMGNCITCEGRV
                     DSQEYANIRSAMKVLMFTDTENWEISKLLAAILHMGNLQYEARTFENLDACEVLFSPSL
                     ATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIV
                     EKINAAIYKPPPLEVKNSRRSIGLLDIFGFENFTVNSFEQLCINFANEHLQQFFVRHVF
                     KLEQEEYDLESIDWLHIEFTDNQEALDMIANRPMNVISLIDEESKFPKGTDATMLHKLN
                     SQHKLNANYVPPKNSHETQFGINHFAGVVYYESQGFLEKNRDTLHGDIIQLVHSSRNKF
                     IKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLF
                     DRHLCVRQLRYSGMMETIRIRHAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQ
                     RMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFL
                     RLKSAATLIQRHWRGHHCRKNYELIRLGFLRLQALHRSRKLHKQYRLARQRIIEFQARC
                     RAYLVRKAFRHRLWAVITVQAYARGMIARRLHRRLRVEYQRRLEAERMRLAEEEKLRKE
                     MSAKKAKEEAERKHQERLAQLAREDAERELKEKEEARRKKELLEQMEKARHEPINHSDM
                     VDKMFGFLGTSGSLPGQEGQAPSGFEDLERGRREMVEEDVDAALPLPDEDEEDLSEYKF
                     AKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTA
                     MSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGETQLPEGQKKTSVRHKLVHLTLKKK
                     SKLTEEVTKRLNDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQ
                     LTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVN
                     GTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTARELCNALADKISLKDR
                     FGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWH
                     NPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLSLVP
                     TYIPDREITPLKNLEKWAQLAIAAHKKGIYAQRRTDSQKVKEDVVNYARFKWPLLFSRF
                     YEAYKFSGPPLPKSDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRGTKMMAPSF
                     TLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAK
                     GDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDCVYVMPTVTLPPREIVALVTMTP
                     DQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSH
                     TREPLKQALLKKILGSEELSQEACMAFVAVLKYMGDYPSKRMRSVNELTDQIFEWALKA
                     EPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCP
                     LAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKA
                     KDFCQNIASRLLLKSSEGFSLFVKIADKVISVPENDFFFDFVRHLTDWIKKARPIKDGI
                     VPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIY
                     RVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFL
                     KLIFKWPTFGSAFFEVKQTTEPNFPEILLIAINKYGVSLIDPRTKDILTTHPFTKISNW
                     SSGNTYFHITIGNLVRGSKLLCETSLGYKMDDLLTSYISQMLTAMSKQRNSRSGR"
     exon            1630..1782
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     misc_feature    1690..3720
                     /label=Motor
     exon            1783..1967
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            1968..2089
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            2090..2232
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            2233..2346
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            2347..2500
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            2501..2577
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            2578..2697
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            2698..2840
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            2841..3051
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            3052..3187
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            3188..3294
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            3295..3432
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     misc_feature    3391..3414
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /note="propagated from UniProtKB/Swiss-Prot (P97479.2);
                     Region: Actin-binding. /evidence=ECO:0000305"
     exon            3433..3591
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            3592..3684
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            3685..3779
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     misc_feature    3730..3792
                     /label=IQ1
     exon            3780..3864
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     misc_feature    3799..3861
                     /label=IQ2
     exon            3865..4083
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     misc_feature    3868..3930
                     /label=IQ3
     misc_feature    3937..3999
                     /label=IQ4
     misc_feature    4006..4068
                     /label=IQ5
     misc_feature    4069..4302
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /note="propagated from UniProtKB/Swiss-Prot (P97479.2);
                     Region: SAH. /evidence=ECO:0000250|UniProtKB:Q13402"
     misc_feature    4069..4302
                     /label=SAH
     exon            4084..4191
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            4192..4401
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            4402..4605
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     misc_feature    4546..5256
                     /label=MYTH4 1
     exon            4606..4782
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            4783..4872
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            4873..5000
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            5001..5127
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            5128..5247
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            5248..5421
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     misc_feature    5269..6189
                     /label=FERM 1
     exon            5422..5649
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            5650..5820
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            5821..5938
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            5939..6065
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     misc_feature    6190..6399
                     /label=SH3
     exon            6236..6426
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            6427..6551
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            6552..6709
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     misc_feature    6622..7071
                     /label=MYTH4 2
     exon            6710..6863
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            6864..7019
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            7020..7125
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     misc_feature    7087..7998
                     /label=FERM 2
     exon            7126..7239
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            7240..7327
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            7328..7434
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            7435..7620
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            7621..7737
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            7738..7821
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            7822..7941
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     misc_feature    7909..7920
                     /label=AGSA
     misc_feature    8039..8046
                     /label=MCS
                     /note="multiple cloning site of fluorescent protein 
                     plasmids"
     misc_feature    8055..8065
                     /label=stop codons
                     /note="stop codons in all three reading frames"
     polyA_signal    8170..8291
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(8298..8753)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        8780..8884
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8886..9243
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9094..9229
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9278..10072
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    10304..10351
                     /label=HSV TK poly(A) signal
                     /note="herpesvirus thymidine kinase polyadenylation signal"
     rep_origin      10680..11268
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcatgg cagaaatcgg tactggcttt ccattcgacc cccattatgt ggaagtcctg
      661 ggcgagcgca tgcactacgt cgatgttggt ccgcgcgatg gcacccctgt gctgttcctg
      721 cacggtaacc cgacctcctc ctacgtgtgg cgcaacatca tcccgcatgt tgcaccgacc
      781 catcgctgca ttgctccaga cctgatcggt atgggcaaat ccgacaaacc agacctgggt
      841 tatttcttcg acgaccacgt ccgcttcatg gatgccttca tcgaagccct gggtctggaa
      901 gaggtcgtcc tggtcattca cgactggggc tccgctctgg gtttccactg ggccaagcgc
      961 aatccagagc gcgtcaaagg tattgcattt atggagttca tccgccctat cccgacctgg
     1021 gacgaatggc cagaatttgc ccgcgagacc ttccaggcct tccgcaccac cgacgtcggc
     1081 cgcaagctga tcatcgatca gaacgttttt atcgagggta cgctgccgat gggtgtcgtc
     1141 cgcccgctga ctgaagtcga gatggaccat taccgcgagc cgttcctgaa tcctgttgac
     1201 cgcgagccac tgtggcgctt cccaaacgag ctgccaatcg ccggtgagcc agcgaacatc
     1261 gtcgcgctgg tcgaagaata catggactgg ctgcaccagt cccctgtccc gaagctgctg
     1321 ttctggggca ccccaggcgt tctgatccca ccggccgaag ccgctcgcct ggccaaaagc
     1381 ctgcctaact gcaaggctgt ggacatcggc ccgggtctga atctgctgca agaagacaac
     1441 ccggacctga tcggcagcga gatcgcgcgc tggctgtcca cgctggagat ttccggctcc
     1501 ggactcagat ctcgagctca agcttcgaat tctatggacc tgaagtcagg ccaggagttt
     1561 gatgtgccca tcggggccgt ggtgaagctc tgcgactcgg gccagatcgt ggtggatgat
     1621 gaagacaatg aacactggat atcccctcag aatgccacgc acatcaagcc aatgcacccc
     1681 acatcggtgc acggcgtgga ggacatgatc cgcctggggg atctcaacga ggcaggcatc
     1741 cttcgaaacc ttctcattcg ctaccgggac cacctcatct atacgtacac aggttccatc
     1801 ctggtggccg tgaaccccta ccagctgctc tccatctact cgccagagca catccgccag
     1861 tacaccaaca agaagatagg ggagatgccc ccccacatct tcgccattgc tgacaactgc
     1921 tacttcaaca tgaaacgcaa caaccgggac cagtgctgta ttatcagcgg ggagtcggga
     1981 gctggcaaga cagagagcac aaagttgatc ctgcagttcc tggcagccat cagtggacag
     2041 cactcatgga tcgagcagca ggtgctggag gccaccccga tcctggaagc atttgggaac
     2101 gccaagacca tccgcaacga caactctagc cgctttggca agtacattga catccacttt
     2161 aacaagcgtg gtgccatcga gggcgccaaa atagagcaat acctgctgga gaagtcacgt
     2221 gtctgccgcc aggcccctga cgagaggaac tatcacgtgt tctactgtat gctggagggc
     2281 atgaatgagg aggagaagaa gaaactgggc ctaggccagg ccgctgacta caactacttg
     2341 gccatgggta actgcatcac ctgtgagggc cgcgtggaca gtcaggagta tgccaacatc
     2401 cgctctgcca tgaaggttct catgttcaca gacacggaga actgggagat ctcgaagctt
     2461 ctggctgcca tcctacacat gggcaatctg cagtatgagg cccggacatt tgagaacttg
     2521 gatgcgtgtg aagtcctctt ctccccatcg ctggccacgg cagcttctct gctcgaggtg
     2581 aaccccccag acctgatgag ctgcctcacc agccgcaccc tcatcacccg tggggagacg
     2641 gtgtccaccc ctctcagcag ggaacaggcg ctggatgtgc gagatgcctt tgtcaagggc
     2701 atctatgggc ggctctttgt gtggattgtg gagaagatca acgcagcaat ctacaagcca
     2761 ccccccctgg aagtgaagaa ctctcgccgg tccatcggtc tcctggacat ctttggattt
     2821 gagaacttca ctgtgaacag cttcgagcag ctctgcatta actttgccaa tgagcacctg
     2881 cagcaattct tcgtgcggca cgtgttcaag ctggagcagg aggagtacga cctggagagc
     2941 atcgactggt tgcacattga gttcactgac aaccaggaag cactggacat gattgccaac
     3001 cggcctatga acgtcatctc cctcatcgat gaggagagca agttccccaa gggcacggat
     3061 gccaccatgc tgcataagct gaactcacag cacaagctca atgccaacta cgtgccaccc
     3121 aagaacagcc acgagaccca gtttggaatc aaccactttg cgggtgttgt ctattatgag
     3181 agtcaaggct tcctggagaa gaaccgagac accctgcatg gggacatcat ccagctggtc
     3241 cactcttccc ggaacaagtt cataaagcag attttccaag ctgacgttgc catgggtgcc
     3301 gagaccagga agcgctcgcc tacactcagc agccagttca agcggtctct ggagctgctg
     3361 atgcgcacac tgggcgcctg ccagcccttc tttgtgcgtt gtatcaaacc caatgagttc
     3421 aagaagccca tgctcttcga ccggcacttg tgtgtacgcc agctgcgata ttcgggcatg
     3481 atggagacaa tccgcatccg ccacgcaggc taccccattc gctacagctt tgtggagttt
     3541 gtggagcgct accgggtact gctgcctggt gtgaagccag catacaagca gggtgacctc
     3601 cgagggacat gccagcgcat ggctgaggct gtgctgggca cgcacgatga ctggcagatt
     3661 ggcaaaacca agatctttct gaaggaccac catgacatgt tgctggaggt ggagcgggac
     3721 aaggccatca cagacagagt cattctcctc cagaaggtta tccggggctt caaagacagg
     3781 tccaacttcc tgagactgaa gagtgctgcc acactgatcc agaggcactg gcggggccac
     3841 cactgtagga aaaactatga gctgattcgt cttggcttcc tgcggctgca ggccctgcac
     3901 cgctcccgga agctgcacaa gcagtaccgc ctggccagac agcgcataat agagttccag
     3961 gcccgctgcc gggcctatct ggtgcgcaag gccttccgcc accgcctctg ggccgtgatc
     4021 accgtgcagg cctatgcccg aggcatgatt gcccgccggc tacaccgccg cctccgggtt
     4081 gagtaccagc ggcgcctcga ggcagagagg atgcgtctgg cagaggagga gaaactccga
     4141 aaggagatga gtgccaagaa ggccaaagag gaggctgagc gcaagcatca ggagcgcctg
     4201 gctcagctag cccgcgagga tgcggagcgg gaactgaagg agaaggagga ggctcggagg
     4261 aagaaggaac tgctggagca gatggagaag gcccgccacg aacccatcaa ccactcagat
     4321 atggtggaca agatgtttgg cttcctgggg acttcaggca gcctgccagg ccaggaaggc
     4381 caggcgccta gtggctttga ggacctagag cgcggacgga gggagatggt ggaagaggat
     4441 gttgacgctg ccctgcccct gcctgatgaa gacgaggagg acctttctga gtacaaattc
     4501 gccaagtttg ctgccaccta cttccagggc acaaccacac actcctacac ccggaggcct
     4561 ctcaagcagc cgctgctcta ccacgacgat gagggtgacc agctggcggc gctggctgtc
     4621 tggatcacca tcctccggtt catgggggac ctcccagagc ccaagtacca cacagccatg
     4681 agcgacggca gtgagaagat cccagtgatg actaagatct acgagaccct aggcaagaag
     4741 acatataaga gggagctgca ggccttgcag ggcgagggcg agacccagct ccctgagggg
     4801 cagaagaaga ccagtgtgag acacaagttg gtacacttga cactgaagaa aaagtccaaa
     4861 ctcacagaag aggtgaccaa gaggctgaac gatggggaat ccacggtaca gggcaacagc
     4921 atgctggagg atcggcccac ctcaaatcta gagaagctgc acttcatcat cggcaacggc
     4981 atcctgcggc ctgcgctgcg ggacgagatt tactgccaga tcagtaagca gctcacacac
     5041 aacccatcca agagcagcta tgccaggggc tggatcctcg tgtcgctctg tgtgggctgc
     5101 ttcgccccct ctgagaagtt cgttaagtac ctgcggaact tcatccacgg aggcccacct
     5161 ggctatgctc cttactgtga ggagcgcctg aggaggacct ttgtcaacgg aactcggaca
     5221 cagccaccca gctggctgga gctgcaggcc accaagtcca agaagcccat catgttgccc
     5281 gtgaccttca tggatgggac caccaagacc ctgctaacag attcagcaac tacagccagg
     5341 gagctgtgca atgctctggc tgacaagatc tcactcaagg accgctttgg cttctccctc
     5401 tacatcgctc tgttcgataa ggtgtcctcc ctgggcagcg gcagtgacca tgtcatggat
     5461 gccatctctc agtgtgagca gtacgccaag gagcagggtg ctcaggagcg caacgcccca
     5521 tggaggctct tctttagaaa ggaggtcttc acaccctggc acaacccctc ggaggacaac
     5581 gtggccacga acctcatcta ccagcaggtg gtgcgaggag tcaagtttgg ggagtacagg
     5641 tgtgagaagg aggacgacct ggctgagctg gcttctcagc agtactttgt ggactatggt
     5701 tctgagatga ttctggagcg cctgctgagc ctcgtgccca cttacatccc tgaccgtgag
     5761 atcacaccgc tgaagaatct tgagaagtgg gcacagctgg ccattgctgc ccacaagaag
     5821 ggaatttatg cccagaggag aactgactcc cagaaggtca aagaggatgt ggtcaattat
     5881 gcccgtttca agtggccctt gctcttctcc aggttttacg aagcttacaa attctcaggc
     5941 cctcccctcc ccaagagcga cgtcatcgtg gctgtcaact ggacgggtgt gtacttcgtg
     6001 gacgagcagg agcaggtgct tctggagctg tccttcccgg agatcatggc tgtgtccagc
     6061 agtaggggaa caaagatgat ggcccccagc tttaccctgg ccaccatcaa aggagatgag
     6121 tacaccttca catccagcaa tgctgaggac atccgtgacc tggtggtcac ctttctggag
     6181 gggctacgga agaggtctaa gtatgtggtg gcactgcagg acaatcctaa ccctgctggt
     6241 gaggagtcag gcttcctcag cttcgccaag ggagacctca tcatccttga ccatgatact
     6301 ggtgagcagg tcatgaactc aggctgggcc aacggcatca acgagaggac caagcagcgc
     6361 ggcgacttcc ccactgactg tgtatacgtc atgcccactg tcaccttgcc accaagggag
     6421 attgtggccc tggtcactat gaccccagac cagaggcagg atgtcgtccg gctcctgcag
     6481 cttcgcacag cagagccaga ggtgcgcgcc aagccctaca cgctagagga gttctcctac
     6541 gactacttca ggcccccacc caagcacacg ctgagccgtg tcatggtgtc caaggcccgc
     6601 ggtaaggaca ggctgtggag ccacacacga gagcccctca agcaggccct gctcaagaag
     6661 atcctgggca gtgaagaact ctcccaggaa gcctgcatgg cctttgtagc tgtgctcaag
     6721 tacatgggcg actacccatc caagaggatg cgatccgtca atgagctcac tgaccagatc
     6781 tttgagtggg cactcaaggc tgagcccctc aaggatgagg cctacgtgca gatcctgaag
     6841 cagctgactg acaatcacat caggtacagc gaagagaggg gctgggaact gctgtggctg
     6901 tgcacgggcc tcttcccgcc cagcaacatc ctcctgcctc atgttcagcg gtttctgcag
     6961 tcccgcaagc actgtcctct tgccattgac tgcctgcaga ggctccagaa agccctgaga
     7021 aatggctccc ggaagtaccc tccgcacctg gtggaggtgg aggccatcca acataagact
     7081 acccagatct tccacaaggt ctacttcccc gatgacacgg acgaggcttt tgaggtggag
     7141 tccagcacca aggccaagga cttctgccag aacatcgcca gccggctgct gctcaagtct
     7201 tccgagggat tcagcctttt tgtcaaaatc gcagataagg tcatcagcgt cccagagaat
     7261 gatttcttct ttgactttgt ccgacacctg acagactgga taaagaaagc acggcccatc
     7321 aaggacggaa tcgtgccctc actaacctac caggtgttct tcatgaagaa gctgtggacc
     7381 accacagtgc cgggcaagga ccccatggct gactccatct tccactatta ccaggaactg
     7441 cccaaatatc tccgaggcta ccacaagtgc acccgggagg aggtgctgca gctgggcgca
     7501 ctcatctaca gggtcaagtt tgaggaggac aaatcctact tccctagcat ccccaagttg
     7561 ctgagggagc tggtacccca ggacctaatc cggcaggtct cacctgatga ctggaaacgg
     7621 tctattgtcg cctacttcaa caaacatgcg gggaagtcca aggaggaagc caagctggcc
     7681 ttcctcaaac tcatcttcaa gtggcccacc tttggctcag ccttctttga ggtgaagcaa
     7741 actacagaac caaacttccc agagattctc ttaattgcca tcaacaagta cggggtcagc
     7801 ctcatcgatc ccagaaccaa ggacatcctg actactcacc ccttcaccaa gatctccaac
     7861 tggagtagtg gcaacaccta cttccacatc accattggga acttggtccg tgggagcaaa
     7921 ctgctctgtg agacatcgct gggatacaaa atggatgatc ttctgacttc ctacatcagc
     7981 cagatgctca cagccatgag caagcagagg aactccagga gcgggaggtg agcgggcccg
     8041 ggatccaccg gatctagata actgatcata atcagccata ccacatttgt agaggtttta
     8101 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     8161 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     8221 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     8281 aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     8341 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     8401 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     8461 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     8521 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     8581 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     8641 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     8701 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
     8761 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     8821 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     8881 gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     8941 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     9001 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     9061 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     9121 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     9181 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     9241 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     9301 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     9361 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     9421 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
     9481 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     9541 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     9601 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     9661 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     9721 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     9781 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
     9841 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     9901 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     9961 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
    10021 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
    10081 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
    10141 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
    10201 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact
    10261 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
    10321 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
    10381 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
    10441 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
    10501 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat
    10561 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
    10621 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
    10681 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
    10741 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
    10801 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
    10861 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
    10921 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
    10981 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    11041 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
    11101 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    11161 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    11221 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    11281 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    11341 ttatcccctg attctgtgga taaccgtatt accgccatgc at
//
