LOCUS       Exported                9546 bp ds-DNA     circular SYN 01-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9546)
  AUTHORS   Friedman Lab
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jan 2, 2026 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9546
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             607..1497
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     misc_feature    1461..1497
                     /label=Primer
     misc_feature    1475..1489
                     /label=Overlap
     misc_feature    1479..1485
                     /label=Replace
     misc_feature    1499..1527
                     /label=MCS
                     /note="multiple cloning site of fluorescent protein 
                     plasmids"
     CDS             1534..6192
                     /codon_start=1
                     /product="myosin VIIa isoform 1"
                     /label=myosin VIIa isoform 1
                     /note="motor protein; alternatively spliced"
                     /protein_id="AAV87212.1"
                     /translation="MDLKSGQEFDVPIGAVVKLCDSGQIQVVDDEDNEHWISPQNATHI
                     KPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYS
                     PEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNNRDQCCIISGESGAGKTESTKLILQF
                     LAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIE
                     QYLLEKSRVCRQAPDERNYHVFYCMLEGMNEEEKKKLGLGQAADYNYLAMGNCITCEGR
                     VDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHMGNLQYEARTFENLDACEVLFSPS
                     LATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWI
                     VEKINAAIYKPPPLEVKNSRRSIGLLDIFGFENFTVNSFEQLCINFANEHLQQFFVRHV
                     FKLEQEEYDLESIDWLHIEFTDNQEALDMIANRPMNVISLIDEESKFPKGTDATMLHKL
                     NSQHKLNANYVPPKNSHETQFGINHFAGVVYYESQGFLEKNRDTLHGDIIQLVHSSRNK
                     FIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPML
                     FDRHLCVRQLRYSGMMETIRIRHAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTC
                     QRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNF
                     LRLKSAATLIQRHWRGHHCRKNYELIRLGFLRLQALHRSRKLHKQYRLARQRIIEFQAR
                     CRAYLVRKAFRHRLWAVITVQAYARGMIARRLHRRLRVEYQRRLEAERMRLAEEEKLRK
                     EMSAKKAKEEAERKHQERLAQLAREDAERELKEKEEARRKKELLEQMEKARHEPINHSD
                     MVDKMFGFLGTSGSLPGQEGQAPSGFEDLERGRREMVEEDVDAALPLPDEDEEDLSEYK
                     FAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHT
                     AMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGETQLPEGQKKTSVRHKLVHLTLKK
                     KSKLTEEVTKRLNDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISK
                     QLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFV
                     NGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTARELCNALADKISLKD
                     RFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPW
                     HNPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLSLV
                     PTYIPDREITPLKNLEKWAQLAIAAHKKGIYAQRRTDSQKVKEDVVNYARFKWPLLFSR
                     FYEAYKFSGPPLPKSDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRGTKMMAPS
                     FTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLR"
     exon            1633..1785
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     misc_feature    1693..3723
                     /label=Motor
     exon            1786..1970
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            1971..2092
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            2093..2235
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            2236..2349
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            2350..2503
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            2504..2580
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            2581..2700
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            2701..2843
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            2844..3054
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            3055..3190
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            3191..3297
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            3298..3435
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     misc_feature    3394..3417
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /note="propagated from UniProtKB/Swiss-Prot (P97479.2);
                     Region: Actin-binding. /evidence=ECO:0000305"
     exon            3436..3594
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            3595..3687
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            3688..3782
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     misc_feature    3733..3795
                     /label=IQ1
     exon            3783..3867
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     misc_feature    3802..3864
                     /label=IQ2
     exon            3868..4086
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     misc_feature    3871..3933
                     /label=IQ3
     misc_feature    3940..4002
                     /label=IQ4
     misc_feature    4009..4071
                     /label=IQ5
     misc_feature    4072..4305
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /note="propagated from UniProtKB/Swiss-Prot (P97479.2);
                     Region: SAH. /evidence=ECO:0000250|UniProtKB:Q13402"
     misc_feature    4072..4305
                     /label=SAH
     exon            4087..4194
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            4195..4404
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            4405..4608
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     misc_feature    4549..5259
                     /label=MYTH4 1
     exon            4609..4785
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            4786..4875
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            4876..5003
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            5004..5130
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            5131..5250
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            5251..5424
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     misc_feature    5272..6192
                     /label=FERM 1
     exon            5425..5652
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            5653..5823
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            5824..5941
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     exon            5942..6068
                     /gene="Myo7a"
                     /gene_synonym="Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B"
                     /inference="alignment:same species:2.1.0"
     misc_feature    6203..6210
                     /label=MCS
                     /note="multiple cloning site of fluorescent protein 
                     plasmids"
     misc_feature    6219..6229
                     /label=stop codons
                     /note="stop codons in all three reading frames"
     polyA_signal    6334..6455
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(6462..6917)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        6944..7048
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7050..7407
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7258..7393
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7442..8236
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    8468..8515
                     /label=HSV TK poly(A) signal
                     /note="herpesvirus thymidine kinase polyadenylation signal"
     rep_origin      8844..9432
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcatgg cagaaatcgg tactggcttt ccattcgacc cccattatgt ggaagtcctg
      661 ggcgagcgca tgcactacgt cgatgttggt ccgcgcgatg gcacccctgt gctgttcctg
      721 cacggtaacc cgacctcctc ctacgtgtgg cgcaacatca tcccgcatgt tgcaccgacc
      781 catcgctgca ttgctccaga cctgatcggt atgggcaaat ccgacaaacc agacctgggt
      841 tatttcttcg acgaccacgt ccgcttcatg gatgccttca tcgaagccct gggtctggaa
      901 gaggtcgtcc tggtcattca cgactggggc tccgctctgg gtttccactg ggccaagcgc
      961 aatccagagc gcgtcaaagg tattgcattt atggagttca tccgccctat cccgacctgg
     1021 gacgaatggc cagaatttgc ccgcgagacc ttccaggcct tccgcaccac cgacgtcggc
     1081 cgcaagctga tcatcgatca gaacgttttt atcgagggta cgctgccgat gggtgtcgtc
     1141 cgcccgctga ctgaagtcga gatggaccat taccgcgagc cgttcctgaa tcctgttgac
     1201 cgcgagccac tgtggcgctt cccaaacgag ctgccaatcg ccggtgagcc agcgaacatc
     1261 gtcgcgctgg tcgaagaata catggactgg ctgcaccagt cccctgtccc gaagctgctg
     1321 ttctggggca ccccaggcgt tctgatccca ccggccgaag ccgctcgcct ggccaaaagc
     1381 ctgcctaact gcaaggctgt ggacatcggc ccgggtctga atctgctgca agaagacaac
     1441 ccggacctga tcggcagcga gatcgcgcgc tggctgtcca cgctggagat ttccggctcc
     1501 ggactcagat ctcgagctca agcttcgaat tctatggacc tgaagtcagg ccaggagttt
     1561 gatgtgccca tcggggccgt ggtgaagctc tgcgactcgg gccagatcca ggtggtggat
     1621 gatgaagaca atgaacactg gatatcccct cagaatgcca cgcacatcaa gccaatgcac
     1681 cccacatcgg tgcacggcgt ggaggacatg atccgcctgg gggatctcaa cgaggcaggc
     1741 atccttcgaa accttctcat tcgctaccgg gaccacctca tctatacgta cacaggttcc
     1801 atcctggtgg ccgtgaaccc ctaccagctg ctctccatct actcgccaga gcacatccgc
     1861 cagtacacca acaagaagat aggggagatg cccccccaca tcttcgccat tgctgacaac
     1921 tgctacttca acatgaaacg caacaaccgg gaccagtgct gtattatcag cggggagtcg
     1981 ggagctggca agacagagag cacaaagttg atcctgcagt tcctggcagc catcagtgga
     2041 cagcactcat ggatcgagca gcaggtgctg gaggccaccc cgatcctgga agcatttggg
     2101 aacgccaaga ccatccgcaa cgacaactct agccgctttg gcaagtacat tgacatccac
     2161 tttaacaagc gtggtgccat cgagggcgcc aaaatagagc aatacctgct ggagaagtca
     2221 cgtgtctgcc gccaggcccc tgacgagagg aactatcacg tgttctactg tatgctggag
     2281 ggcatgaatg aggaggagaa gaagaaactg ggcctaggcc aggccgctga ctacaactac
     2341 ttggccatgg gtaactgcat cacctgtgag ggccgcgtgg acagtcagga gtatgccaac
     2401 atccgctctg ccatgaaggt tctcatgttc acagacacgg agaactggga gatctcgaag
     2461 cttctggctg ccatcctaca catgggcaat ctgcagtatg aggcccggac atttgagaac
     2521 ttggatgcgt gtgaagtcct cttctcccca tcgctggcca cggcagcttc tctgctcgag
     2581 gtgaaccccc cagacctgat gagctgcctc accagccgca ccctcatcac ccgtggggag
     2641 acggtgtcca cccctctcag cagggaacag gcgctggatg tgcgagatgc ctttgtcaag
     2701 ggcatctatg ggcggctctt tgtgtggatt gtggagaaga tcaacgcagc aatctacaag
     2761 ccaccccccc tggaagtgaa gaactctcgc cggtccatcg gtctcctgga catctttgga
     2821 tttgagaact tcactgtgaa cagcttcgag cagctctgca ttaactttgc caatgagcac
     2881 ctgcagcaat tcttcgtgcg gcacgtgttc aagctggagc aggaggagta cgacctggag
     2941 agcatcgact ggttgcacat tgagttcact gacaaccagg aagcactgga catgattgcc
     3001 aaccggccta tgaacgtcat ctccctcatc gatgaggaga gcaagttccc caagggcacg
     3061 gatgccacca tgctgcataa gctgaactca cagcacaagc tcaatgccaa ctacgtgcca
     3121 cccaagaaca gccacgagac ccagtttgga atcaaccact ttgcgggtgt tgtctattat
     3181 gagagtcaag gcttcctgga gaagaaccga gacaccctgc atggggacat catccagctg
     3241 gtccactctt cccggaacaa gttcataaag cagattttcc aagctgacgt tgccatgggt
     3301 gccgagacca ggaagcgctc gcctacactc agcagccagt tcaagcggtc tctggagctg
     3361 ctgatgcgca cactgggcgc ctgccagccc ttctttgtgc gttgtatcaa acccaatgag
     3421 ttcaagaagc ccatgctctt cgaccggcac ttgtgtgtac gccagctgcg atattcgggc
     3481 atgatggaga caatccgcat ccgccacgca ggctacccca ttcgctacag ctttgtggag
     3541 tttgtggagc gctaccgggt actgctgcct ggtgtgaagc cagcatacaa gcagggtgac
     3601 ctccgaggga catgccagcg catggctgag gctgtgctgg gcacgcacga tgactggcag
     3661 attggcaaaa ccaagatctt tctgaaggac caccatgaca tgttgctgga ggtggagcgg
     3721 gacaaggcca tcacagacag agtcattctc ctccagaagg ttatccgggg cttcaaagac
     3781 aggtccaact tcctgagact gaagagtgct gccacactga tccagaggca ctggcggggc
     3841 caccactgta ggaaaaacta tgagctgatt cgtcttggct tcctgcggct gcaggccctg
     3901 caccgctccc ggaagctgca caagcagtac cgcctggcca gacagcgcat aatagagttc
     3961 caggcccgct gccgggccta tctggtgcgc aaggccttcc gccaccgcct ctgggccgtg
     4021 atcaccgtgc aggcctatgc ccgaggcatg attgcccgcc ggctacaccg ccgcctccgg
     4081 gttgagtacc agcggcgcct cgaggcagag aggatgcgtc tggcagagga ggagaaactc
     4141 cgaaaggaga tgagtgccaa gaaggccaaa gaggaggctg agcgcaagca tcaggagcgc
     4201 ctggctcagc tagcccgcga ggatgcggag cgggaactga aggagaagga ggaggctcgg
     4261 aggaagaagg aactgctgga gcagatggag aaggcccgcc acgaacccat caaccactca
     4321 gatatggtgg acaagatgtt tggcttcctg gggacttcag gcagcctgcc aggccaggaa
     4381 ggccaggcgc ctagtggctt tgaggaccta gagcgcggac ggagggagat ggtggaagag
     4441 gatgttgacg ctgccctgcc cctgcctgat gaagacgagg aggacctttc tgagtacaaa
     4501 ttcgccaagt ttgctgccac ctacttccag ggcacaacca cacactccta cacccggagg
     4561 cctctcaagc agccgctgct ctaccacgac gatgagggtg accagctggc ggcgctggct
     4621 gtctggatca ccatcctccg gttcatgggg gacctcccag agcccaagta ccacacagcc
     4681 atgagcgacg gcagtgagaa gatcccagtg atgactaaga tctacgagac cctaggcaag
     4741 aagacatata agagggagct gcaggccttg cagggcgagg gcgagaccca gctccctgag
     4801 gggcagaaga agaccagtgt gagacacaag ttggtacact tgacactgaa gaaaaagtcc
     4861 aaactcacag aagaggtgac caagaggctg aacgatgggg aatccacggt acagggcaac
     4921 agcatgctgg aggatcggcc cacctcaaat ctagagaagc tgcacttcat catcggcaac
     4981 ggcatcctgc ggcctgcgct gcgggacgag atttactgcc agatcagtaa gcagctcaca
     5041 cacaacccat ccaagagcag ctatgccagg ggctggatcc tcgtgtcgct ctgtgtgggc
     5101 tgcttcgccc cctctgagaa gttcgttaag tacctgcgga acttcatcca cggaggccca
     5161 cctggctatg ctccttactg tgaggagcgc ctgaggagga cctttgtcaa cggaactcgg
     5221 acacagccac ccagctggct ggagctgcag gccaccaagt ccaagaagcc catcatgttg
     5281 cccgtgacct tcatggatgg gaccaccaag accctgctaa cagattcagc aactacagcc
     5341 agggagctgt gcaatgctct ggctgacaag atctcactca aggaccgctt tggcttctcc
     5401 ctctacatcg ctctgttcga taaggtgtcc tccctgggca gcggcagtga ccatgtcatg
     5461 gatgccatct ctcagtgtga gcagtacgcc aaggagcagg gtgctcagga gcgcaacgcc
     5521 ccatggaggc tcttctttag aaaggaggtc ttcacaccct ggcacaaccc ctcggaggac
     5581 aacgtggcca cgaacctcat ctaccagcag gtggtgcgag gagtcaagtt tggggagtac
     5641 aggtgtgaga aggaggacga cctggctgag ctggcttctc agcagtactt tgtggactat
     5701 ggttctgaga tgattctgga gcgcctgctg agcctcgtgc ccacttacat ccctgaccgt
     5761 gagatcacac cgctgaagaa tcttgagaag tgggcacagc tggccattgc tgcccacaag
     5821 aagggaattt atgcccagag gagaactgac tcccagaagg tcaaagagga tgtggtcaat
     5881 tatgcccgtt tcaagtggcc cttgctcttc tccaggtttt acgaagctta caaattctca
     5941 ggccctcccc tccccaagag cgacgtcatc gtggctgtca actggacggg tgtgtacttc
     6001 gtggacgagc aggagcaggt gcttctggag ctgtctttcc cggagatcat ggctgtgtcc
     6061 agcagtaggg gaacaaagat gatggccccc agctttaccc tggccaccat caaaggagat
     6121 gagtacacct tcacatccag caatgctgag gacatccgtg acctggtggt cacctttctg
     6181 gaggggctac ggtaagcggg cccgggatcc accggatcta gataactgat cataatcagc
     6241 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     6301 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     6361 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6421 agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat
     6481 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     6541 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     6601 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     6661 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     6721 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     6781 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     6841 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     6901 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     6961 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     7021 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg
     7081 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     7141 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     7201 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     7261 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     7321 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     7381 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg
     7441 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     7501 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     7561 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     7621 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     7681 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     7741 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     7801 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     7861 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     7921 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
     7981 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     8041 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     8101 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     8161 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     8221 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     8281 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     8341 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     8401 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
     8461 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa
     8521 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
     8581 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
     8641 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata
     8701 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     8761 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     8821 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     8881 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     8941 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     9001 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     9061 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     9121 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     9181 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     9241 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     9301 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     9361 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     9421 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     9481 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     9541 atgcat
//
