LOCUS       pGEF-U.dna             17180 bp ds-DNA     circular SYN 21-OCT-2018
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 21, 2018 from SnapGene Viewer 4.2.3 to Vector NTI(R) 
            format
            http://www.snapgene.com
COMMENT     VNTDATE|853632000|
COMMENT     VNTDBDATE|856137600|
COMMENT     VNTNAME|pGEF-U.dna|
FEATURES             Location/Qualifiers
     misc_feature    107..131
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    215..566
                     /label=LIR
     protein_bind    595..616
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        631..661
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer          662..681
                     /label=TNoS-GWB-Rev
                     /note="sequence: tgtggaattgtgagcggata"
     protein_bind    669..685
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     693..709
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      743..995
                     /label=tNOS
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(1020..1739)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=eGFP
                     /note="mammalian codon-optimized"
     protein_bind    1744..1768
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(1773..2118)
                     /label=P35S
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer          complement(2069..2088)
                     /label=P35S-Univ-For
                     /note="sequence: aaacctcctcggattccatt"
     primer          complement(2370..2389)
                     /label=AscI-DownStream
                     /note="sequence: gaagcaagccttgaatcgtc"
     promoter        2637..2982
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer          2667..2686
                     /label=P35S-Univ-For
                     /note="sequence: aaacctcctcggattccatt"
     CDS             3016..7119
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
     CDS             7132..7152
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
     misc_feature    7162..7411
                     /label=HSP-terminator
     misc_feature    7439..7862
                     /label=U6-26p
     primer          7865..7884
                     /label=BsaI-Spec-Fw
                     /note="sequence: cgggggagtcgagaccAACCCAGTGGACAT"
     CDS             8142..8933
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
     primer          complement(9063..9084)
                     /label=BsaI-Spec-Rv
                     /note="sequence: gggaaattcGAGACCGGCTTATTATGCACG"
     misc_RNA        9085..9160
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    9161..9352
                     /label=U6-26t
     misc_feature    9362..9696
                     /label=SIR
     misc_feature    9697..10636
                     /label=REP/REPA
     misc_feature    10637..10988
                     /label=LIR
     misc_feature    11010..11034
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             12334..12963
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
     CDS             13397..14464
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
     rep_origin      14530..14724
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    15068..15208
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(15394..15982)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer          16060..16086
                     /label=HindIII-KanR-R
                     /note="sequence: attaaagcttctaggtactaaaacaattcatccag"
     CDS             complement(16069..16863)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
     primer          complement(16840..16863)
                     /label=HindIII-KanR-For
                     /note="sequence: attaaagcttatggctaaaatgagaatatcaccg"
     primer          17136..17155
                     /label=AscI-UpStream
                     /note="sequence: gttgccgttcttccgaatag"
ORIGIN
        1 cgccttacaa cggctctccc ctgacgccgt cccggactga tgggctgcct gtatcgagtg
       61 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt
      121 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt
      181 agagctcaaa gtttaacgcg ttagcagaag gcatgttgtt gtgactccga ggggttgcct
      241 caaactctat cttataaccg gcgtggaggc atggaggcag gggtattttg gtcattttaa
      301 tagatagtgg aaaatgacgt ggaatttact taaagacgaa gtctttgcga caaggggggg
      361 cccacgccga atttaatatt accggcgtgg ccccccctta tcgcgagtgc tttagcacga
      421 gcggtccaga tttaaagtag aaaatttccc gcccactagg gttaaaggtg ttcacactat
      481 aaaagcatat acgatgtgat ggtatttgat ggagcgtata ttgtatcagg tatttccgtt
      541 ggatacgaat tattcgtacg accctcggta ccgatcggcg cgcctaattc agcctaatgt
      601 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      661 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacga
      721 attggttccg gaaccaattc ccgatctagt aacatagatg acaccgcgcg cgataattta
      781 tcctagtttg cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact
      841 ctaatcataa aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc
      901 ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat
      961 cttaagaaac tttattgcca aatgtttgaa cgatcgggga aattcgagct cgggccgctt
     1021 tacttgtaca gctcgtccat gccgtgagtg atcccggcgg cggtcacgaa ctccagcagg
     1081 accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactgggt gctcaggtag
     1141 tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg
     1201 gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttcac cttgatgccg
     1261 ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg
     1321 tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc
     1381 agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag
     1441 aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc
     1501 ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt aggtgaaggt ggtcacgagg
     1561 gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg
     1621 taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt cacgtcgccg
     1681 tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatg
     1741 atcaccactt tgtacaagaa agctgggtcc cgtgttctct ccaaatgaaa tgaacttcct
     1801 tatatagagg aagggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg
     1861 gagatatcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg
     1921 atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttcaacg
     1981 atggcctttc ctttatcgca atgatggcat ttgtaggagc caccttcctt ttccactatc
     2041 ttcacaataa agtgacagat agctgggcaa tggaatccga ggaggtttcc ggatattacc
     2101 ctttgttgaa aagtctcaat tgccctttgg tcttctgaga cggcgcgcca gatttgcctt
     2161 ttcaatttca gaaagaatgc taacccacag atggttagag aggcttacgc agcaggtatc
     2221 atcaagacga tctacccgag caataatctc caggaaatca aataccttcc caagaaggtt
     2281 aaagatgcag tcaaaagatt caggactaac tgcatcaaga acacagagaa agatatattt
     2341 ctcaagatca gaagtactat tccagtatgg acgattcaag gcttgcttca caaaccaagg
     2401 caagtaatag agattggagt ctctaaaaag gtagttccca ctgaatcaaa ggccatggag
     2461 tcaaagattc aaatagagga cctaacagaa ctcgccgtaa agactggcga acagttcata
     2521 cagagtctct tacgactcaa tgacaagaag aaaatcttcg tcaacatggt ggagcacgac
     2581 acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag ggcaattgag
     2641 acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt
     2701 cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat
     2761 aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca
     2821 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
     2881 tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac
     2941 ccttcctcta tataaggaag ttcatttcat ttggagagaa cacgggggac tcctgcaggt
     3001 agatcgctcg tcgacatgga taagaagtac tctatcggac tcgatatcgg aactaactct
     3061 gtgggatggg ctgtgatcac cgatgagtac aaggtgccat ctaagaagtt caaggttctc
     3121 ggaaacaccg ataggcactc tatcaagaaa aaccttatcg gtgctctcct cttcgattct
     3181 ggtgaaactg ctgaggctac cagactcaag agaaccgcta gaagaaggta caccagaaga
     3241 aagaacagga tctgctacct ccaagagatc ttctctaacg agatggctaa agtggatgat
     3301 tcattcttcc acaggctcga agagtcattc ctcgtggaag aagataagaa gcacgagagg
     3361 caccctatct tcggaaacat cgttgatgag gtggcatacc acgagaagta ccctactatc
     3421 taccacctca gaaagaagct cgttgattct actgataagg ctgatctcag gctcatctac
     3481 ctcgctctcg ctcacatgat caagttcaga ggacacttcc tcatcgaggg tgatctcaac
     3541 cctgataact ctgatgtgga taagttgttc atccagctcg tgcagaccta caaccagctt
     3601 ttcgaagaga accctatcaa cgcttcaggt gtggatgcta aggctatcct ctctgctagg
     3661 ctctctaagt caagaaggct tgagaacctc attgctcagc tccctggtga gaagaagaac
     3721 ggacttttcg gaaacttgat cgctctctct ctcggactca cccctaactt caagtctaac
     3781 ttcgatctcg ctgaggatgc aaagctccag ctctcaaagg atacctacga tgatgatctc
     3841 gataacctcc tcgctcagat cggagatcag tacgctgatt tgttcctcgc tgctaagaac
     3901 ctctctgatg ctatcctcct cagtgatatc ctcagagtga acaccgagat caccaaggct
     3961 ccactctcag cttctatgat caagagatac gatgagcacc accaggatct cacacttctc
     4021 aaggctcttg ttagacagca gctcccagag aagtacaaag agattttctt cgatcagtct
     4081 aagaacggat acgctggtta catcgatggt ggtgcatctc aagaagagtt ctacaagttc
     4141 atcaagccta tcctcgagaa gatggatgga accgaggaac tcctcgtgaa gctcaataga
     4201 gaggatcttc tcagaaagca gaggaccttc gataacggat ctatccctca tcagatccac
     4261 ctcggagagt tgcacgctat ccttagaagg caagaggatt tctacccatt cctcaaggat
     4321 aacagggaaa agattgagaa gattctcacc ttcagaatcc cttactacgt gggacctctc
     4381 gctagaggaa actcaagatt cgcttggatg accagaaagt ctgaggaaac catcacccct
     4441 tggaacttcg aagaggtggt ggataagggt gctagtgctc agtctttcat cgagaggatg
     4501 accaacttcg ataagaacct tccaaacgag aaggtgctcc ctaagcactc tttgctctac
     4561 gagtacttca ccgtgtacaa cgagttgacc aaggttaagt acgtgaccga gggaatgagg
     4621 aagcctgctt ttttgtcagg tgagcaaaag aaggctatcg ttgatctctt gttcaagacc
     4681 aacagaaagg tgaccgtgaa gcagctcaaa gaggattact tcaagaaaat cgagtgcttc
     4741 gattcagttg agatttctgg tgttgaggat aggttcaacg catctctcgg aacctaccac
     4801 gatctcctca agatcattaa ggataaggat ttcttggata acgaggaaaa cgaggatatc
     4861 ttggaggata tcgttcttac cctcaccctc tttgaagata gagagatgat tgaagaaagg
     4921 ctcaagacct acgctcatct cttcgatgat aaggtgatga agcagttgaa gagaagaaga
     4981 tacactggtt ggggaaggct ctcaagaaag ctcattaacg gaatcaggga taagcagtct
     5041 ggaaagacaa tccttgattt cctcaagtct gatggattcg ctaacagaaa cttcatgcag
     5101 ctcatccacg atgattctct cacctttaaa gaggatatcc agaaggctca ggtttcagga
     5161 cagggtgata gtctccatga gcatatcgct aacctcgctg gatctcctgc aatcaagaag
     5221 ggaatcctcc agactgtgaa ggttgtggat gagttggtga aggtgatggg aaggcataag
     5281 cctgagaaca tcgtgatcga aatggctaga gagaaccaga ccactcagaa gggacagaag
     5341 aactctaggg aaaggatgaa gaggatcgag gaaggtatca aagagcttgg atctcagatc
     5401 ctcaaagagc accctgttga gaacactcag ctccagaatg agaagctcta cctctactac
     5461 ctccagaacg gaagggatat gtatgtggat caagagttgg atatcaacag gctctctgat
     5521 tacgatgttg atcatatcgt gccacagtca ttcttgaagg atgattctat cgataacaag
     5581 gtgctcacca ggtctgataa gaacaggggt aagagtgata acgtgccaag tgaagaggtt
     5641 gtgaagaaaa tgaagaacta ttggaggcag ctcctcaacg ctaagctcat cactcagaga
     5701 aagttcgata acttgactaa ggctgagagg ggaggactct ctgaattgga taaggcagga
     5761 ttcatcaaga ggcagcttgt ggaaaccagg cagatcacta agcacgttgc acagatcctc
     5821 gattctagga tgaacaccaa gtacgatgag aacgataagt tgatcaggga agtgaaggtt
     5881 atcaccctca agtcaaagct cgtgtctgat ttcagaaagg atttccaatt ctacaaggtg
     5941 agggaaatca acaactacca ccacgctcac gatgcttacc ttaacgctgt tgttggaacc
     6001 gctctcatca agaagtatcc taagctcgag tcagagttcg tgtacggtga ttacaaggtg
     6061 tacgatgtga ggaagatgat cgctaagtct gagcaagaga tcggaaaggc taccgctaag
     6121 tatttcttct actctaacat catgaatttc ttcaagaccg agattaccct cgctaacggt
     6181 gagatcagaa agaggccact catcgagaca aacggtgaaa caggtgagat cgtgtgggat
     6241 aagggaaggg atttcgctac cgttagaaag gtgctctcta tgccacaggt gaacatcgtt
     6301 aagaaaaccg aggtgcagac cggtggattc tctaaagagt ctatcctccc taagaggaac
     6361 tctgataagc tcattgctag gaagaaggat tgggacccta agaaatacgg tggtttcgat
     6421 tctcctaccg tggcttactc tgttctcgtt gtggctaagg ttgagaaggg aaagagtaag
     6481 aagctcaagt ctgttaagga acttctcgga atcactatca tggaaaggtc atctttcgag
     6541 aagaacccaa tcgatttcct cgaggctaag ggatacaaag aggttaagaa ggatctcatc
     6601 atcaagctcc caaagtactc actcttcgaa ctcgagaacg gtagaaagag gatgctcgct
     6661 tctgctggtg agcttcaaaa gggaaacgag cttgctctcc catctaagta cgttaacttt
     6721 ctttacctcg cttctcacta cgagaagttg aagggatctc cagaagataa cgagcagaag
     6781 caacttttcg ttgagcagca caagcactac ttggatgaga tcatcgagca gatctctgag
     6841 ttctctaaaa gggtgatcct cgctgatgca aacctcgata aggtgttgtc tgcttacaac
     6901 aagcacagag ataagcctat cagggaacag gcagagaaca tcatccatct cttcaccctt
     6961 accaacctcg gtgctcctgc tgctttcaag tacttcgata caaccatcga taggaagaga
     7021 tacacctcta ccaaagaagt gctcgatgct accctcatcc atcagtctat cactggactc
     7081 tacgagacta ggatcgatct ctcacagctc ggtggtgatt caagggctga tcctaagaag
     7141 aagaggaagg tttgactcga gatatgaaga tgaagatgaa atatttggtg tgtcaaataa
     7201 aaagcttgtg tgcttaagtt tgtgtttttt tcttggcttg ttgtgttatg aatttgtggc
     7261 tttttctaat attaaatgaa tgtaagatca cattataatg aataaacaaa tgtttctata
     7321 atccattgtg aatgttttgt tggatctctt ctgcagcata taactactgt atgtgctatg
     7381 gtatggacta tggaatatga ttaaagataa ggagctccgg tgacggaccc atgggcttcg
     7441 acttgccttc cgcacaatac atcatttctt cttagctttt tttcttcttc ttcgttcata
     7501 cagttttttt ttgtttatca gcttacattt tcttgaaccg tagctttcgt tttcttcttt
     7561 ttaactttcc attcggagtt tttgtatctt gtttcatagt ttgtcccagg attagaatga
     7621 ttaggcatcg aaccttcaag aatttgattg aataaaacat cttcattctt aagatatgaa
     7681 gataatcttc aaaaggcccc tgggaatctg aaagaagaga agcaggccca tttatatggg
     7741 aaagaacaat agtatttctt atataggccc atttaagttg aaaacaatct tcaaaagtcc
     7801 cacatcgctt agataagaaa acgaagctga gtttatatac agctagagtc gaagtagtga
     7861 ttgggagacc aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc
     7921 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     7981 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
     8041 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     8101 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgggggaa gcggtgatcg
     8161 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     8221 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
     8281 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     8341 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     8401 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     8461 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     8521 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     8581 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     8641 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     8701 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     8761 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     8821 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     8881 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taatgtctag
     8941 ctagaaattc gttcaagccg acgccgcttc gcggcgcggc ttaactcaag cgttagatgc
     9001 actaagcaca taattgctca cagccaaact atcaggtcaa gtctgctttt attattttta
     9061 agcgtgcata ataagccggt ctcggtttta gagctagaaa tagcaagtta aaataaggct
     9121 agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttttttgc aaaattttcc
     9181 agatcgattt cttcttcctc tgttcttcgg cgttcaattt ctggggtttt ctcttcgttt
     9241 tctgtaactg aaacctaaaa tttgacctaa aaaaaatctc aaataatatg attcagtggt
     9301 tttgtacttt tcagttagtt gagttttgca gttccgatga gataaaccaa taagcttgac
     9361 cgagtgtact tcaagtcagt gggaaatcaa taaaatgatt attttatgaa tatatttcat
     9421 tgtgcaagta gatagaaatt acatatgtta cataacacac gaaataaaca aaaaaagaca
     9481 atccaaaaac aaacacccca aaaaaaataa tcactttaga taaactcgta tgaggagagg
     9541 cacgttcagt gactcgacga ttcccgagca aaaaaagtct ccccgtcaca catgtagtgg
     9601 gtgacgcaat tatctttaaa gtaatccttc tgttgacttg tcattgataa catccagtct
     9661 tcgtcaggat tgcaaagaat tatagaaggg atcccacctt ttattttctt cttttttcca
     9721 tatttagggt tgacagtgaa atcagactgg caacctatta attgcttcca caatgggacg
     9781 aacttgaagg ggatgtcgtc gatgatatta taggtggcgt gttcatcgta gttggtgaaa
     9841 tcgatggtac cgttccaata gttgtgtcgt ccgagacttc tagcccaggt ggtctttccg
     9901 gtacgagttg gtccgcagat gtagaggctg gggtgtcgga ttccattcct tccattgtcc
     9961 ttgttaaatc ggccatccat tcaaggtcag attgagcttg ttggtatgag acaggatgta
    10021 tgtaagtata agcgtctatg cttacatggt atagatgggt ttccctccag gagtgtagat
    10081 cttcgtggca gcgaagatct gattctgtga agggcgacac atacggttca ggttgtggag
    10141 ggaataattt gttggctgaa tattccagcc attgaagctt tgttgcccat tcatgaggga
    10201 attcttcctt gatcatgtca agatattcct ccttagacgt tgcagtctgg ataatagttc
    10261 tccatcgtgc gtcagatttg cgaggagaaa ccttatgatc tcggaaatct cctctggttt
    10321 taatatctcc gtcctttgat atgtaatcaa ggacttgttt agagtttcta gctggctgga
    10381 tattagggtg atttccttca aaatcgaaaa aagaaggatc cctaatacaa ggttttttat
    10441 caagctggag aagagcatga tagtgggtag tgccatcttg atgaagctca gaagcaacac
    10501 caaggaagaa aataagaaaa ggtgtgagtt tctcccagag aaactggaat aaatcatctc
    10561 tttgagatga gcacttggga taggtaagga aaacatattt agattggagt ctgaagttct
    10621 tactagcaga aggcatgttg ttgtgactcc gaggggttgc ctcaaactct atcttataac
    10681 cggcgtggag gcatggaggc aggggtattt tggtcatttt aatagatagt ggaaaatgac
    10741 gtggaattta cttaaagacg aagtctttgc gacaaggggg ggcccacgcc gaatttaata
    10801 ttaccggcgt ggccccccct tatcgcgagt gctttagcac gagcggtcca gatttaaagt
    10861 agaaaatttc ccgcccacta gggttaaagg tgttcacact ataaaagcat atacgatgtg
    10921 atggtatttg atggagcgta tattgtatca ggtatttccg ttggatacga attattcgta
    10981 cgaccctcat agtttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga
    11041 gaaaagagcg tttattagaa taacggatat ttaaaagggc gtgaaaaggt ttatccgttc
    11101 gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttgatcc
    11161 aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa
    11221 acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc
    11281 gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac
    11341 attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg
    11401 acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt
    11461 tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc
    11521 acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc
    11581 gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg
    11641 cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg
    11701 gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg
    11761 ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc
    11821 acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg
    11881 gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg
    11941 aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg
    12001 ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga
    12061 accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga
    12121 gccgcccgcg cacggctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc
    12181 caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa
    12241 aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg
    12301 atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag
    12361 aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc
    12421 ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc
    12481 gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac
    12541 gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac
    12601 ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct
    12661 tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg
    12721 gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc
    12781 ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg
    12841 cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc
    12901 gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt
    12961 tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc
    13021 cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga
    13081 agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac
    13141 gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa
    13201 tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat
    13261 caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg
    13321 ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg gcactggaac ccccaagccc
    13381 gaggaatcgg cgtgacggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg
    13441 tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc
    13501 agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg
    13561 gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc
    13621 agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga
    13681 cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga
    13741 gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga
    13801 ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga
    13861 agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt
    13921 ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt
    13981 aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac
    14041 ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg
    14101 gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa
    14161 gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg
    14221 ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa
    14281 gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg
    14341 caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc
    14401 tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc
    14461 ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg
    14521 cctctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa
    14581 cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga
    14641 tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct
    14701 taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa
    14761 agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc
    14821 ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca
    14881 agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt
    14941 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggaaacggtc acagcttgtc
    15001 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
    15061 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
    15121 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
    15181 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
    15241 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
    15301 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
    15361 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
    15421 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
    15481 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
    15541 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
    15601 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
    15661 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    15721 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    15781 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
    15841 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    15901 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    15961 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    16021 gtggaacgaa aactcacgtt aagggatttt ggtcatgcat tctaggtact aaaacaattc
    16081 atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa
    16141 aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc
    16201 aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt
    16261 gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc
    16321 ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc
    16381 ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc
    16441 ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa
    16501 gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata
    16561 ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg
    16621 ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc
    16681 cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata
    16741 taggttttca ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc
    16801 ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc
    16861 catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat
    16921 aacaagacga actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca
    16981 gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg
    17041 aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc
    17101 cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg
    17161 gtaacatgag caaagtctgc
//
