LOCUS       PSY243-pAG415GPD-SU9MT 12214 bp ds-DNA     circular     06-JAN-2026
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          67..96
                     /label="VSY3-seq-F12"
                     /note="sequence: ggtttgtttgccggatcaagagctaccaac"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          828..850
                     /label="VSY3-seq-F13"
                     /note="sequence: ggcacgacaggtttcccgactgg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_binding    947..969
                     /label="LacO"
                     /ApEinfo_revcolor="#7ea7d8"
                     /ApEinfo_fwdcolor="#7ea7d8"
     primer_bind     975..995
                     /label="M13-rev"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     promoter        1012..1030
                     /label="T3 promoter"
                     /ApEinfo_revcolor="#8882be"
                     /ApEinfo_fwdcolor="#8882be"
     primer_bind     1013..1032
                     /label="T3"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     primer          1046..1086
                     /label="D142-F1"
                     /note="sequence: ctcagtttatcattatcaatactcgccatttcaaagaatac"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    1049..1697
                     /label="GPD_promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          1572..1621
                     /label="SY32-seq-F1-GPDF"
                     /note="sequence: GACTAATAAGTATATAAAGACGGTAGGTATTGATTGTAATTCTGTAAATC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          1683..1749
                     /label="D2201-a"
                     /note="sequence: cagaacttagtttcgacggattctagaactagtggatcccccatcacaagtttgtacaaaaaagcAG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          1687..1766
                     /label="D205-Fa"
                     /note="sequence: acttagtttcgacggattctagaactagtggatcccccatcacaagtttgtacaaaaaagcAGGCTTCGAAGGAGATAAC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          1690..1716
                     /label="D243-F4"
                     /note="sequence: tagtttcgacggattctagaactagtg"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          1690..1739
                     /label="E1-F1"
                     /note="sequence: tagtttcgacggattctagaactagtggatcccccatcacaagtttgtac"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          1720..1761
                     /label="D206-FF1"
                     /note="sequence: cccccatcacaagTTTGTACAAAAAAGCAGGCTTCGAAGGAG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          1721..1790
                     /label="D205-F1"
                     /note="sequence: ccccatcacaagtttgtacaaaaaagcAGGCTTCGAAGGAGATAACAAAATGGCCTCCACTCGTGTCCTC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1728..1752
                     /label="attB1_invitrogen"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_recomb     1729..1747
                     /label="attB1"
                     /ApEinfo_revcolor="#bc8dbf"
                     /ApEinfo_fwdcolor="#bc8dbf"
     misc_recomb     1729..1747
                     /label="attR1"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1732..1751
                     /label="ATTB1 primer"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_recomb     1733..1747
                     /label="attB1_short"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1736..1766
                     /label="GUF"
                     /note="sequence: GTACAAAAAAGCAGGCTTCGAAGGAGATAAC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          1770..1849
                     /label="D129-Fa"
                     /note="sequence: ATGGCCTCCACTCGTGTCCTCGCCTCTCGCCTGGCCTCCCAGATGGCTGCTTCCGCCAAGGTTGCCCGCCCTGCTGTCCG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1770..1976
                     /label="SU9MTS"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(1833..1912)
                     /label="D129-Fb"
                     /note="sequence: GTGCGCTTGAGGGTCTGGAGGGGGGAGCCAGTCTGGATGGTGCGCTTGCTGACCTGAGCAACGCGGACAGCAGGGCGGGC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(1893..1972)
                     /label="D129-Rd"
                     /note="sequence: GAGTAGGCGCGCTTCTGGAAAGCCTGGCGGGTGGTGGCGTTGACGATGGAGGTCATCTGGGTGCGCTTGAGGGTCTGGAG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          1953..2003
                     /label="D2204-F"
                     /note="sequence: TTCCAGAAGCGCGCCTACTCTTCCtctgaggtggagttttcccacgagtac"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1977..2474
                     /label="max10"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             1977..6302
                     /label="Translation 1977-6302"
                     /translation="SEVEFSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWNRAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTFEPCVMCAGAMIHSRIGRVVFGVRNSKRGAAGSLMNVLNYPGMNHRVEITEGILADECAALLCDFYRMPRQVFNAQKKAQSSINSGGSSGGSSGSETPGTSESATPESSGGSSGGSSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAEDYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEIAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFSEEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGEFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLSDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNGPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQLQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDLLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSKDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGNYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLIDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVEEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKEELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKINTEVRVLLKHDDNPYVIGIARGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDYHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSGFKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQNGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDYKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNYEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSVKSGGSGKPIPNPLLGLDST"
     primer          complement(2006..2026)
                     /label="D243-R4"
                     /note="sequence: agggtcagggcatgtctcatc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          2007..2027
                     /label="D243-F1"
                     /note="sequence: atgagacatgccctgaccctg"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(2456..2475)
                     /label="KZ1428"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             2475..2570
                     /label="Genscript BE4 linker"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2513..2529
                     /label="USER"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     2513..2594
                     /label="MFR 549"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     complement(2526..2570)
                     /label="851_genscript_rAPOBEC1-rev"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     gene            2571..6248
                     /label="LbCpf1"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(2745..2769)
                     /label="KZ557"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer_bind     2919..2941
                     /label="SeqJH132"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     3519..3537
                     /label="SeqJH133"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(3701..3719)
                     /label="SeqJH140"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     4119..4142
                     /label="SeqJH134"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          4166..4194
                     /label="D243-F2"
                     /note="sequence: ctgggacaaggataaggagacagactatc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(4166..4194)
                     /label="D243-R1"
                     /note="sequence: gatagtctgtctccttatccttgtcccag"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(4398..4419)
                     /label="SeqJH141"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     4616..4640
                     /label="KZ562"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     4719..4739
                     /label="SeqJH135"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(5036..5080)
                     /label="JI180"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    5061..5063
                     /label="D832A if want nuclease inactive"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    5061..5084
                     /label="JI181"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(5104..5119)
                     /label="SeqJH142"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     5319..5336
                     /label="SeqJH136"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(5799..5819)
                     /label="SeqJH143"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     5919..5938
                     /label="SeqJH137"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     6116..6140
                     /label="KZ566"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    6166..6182
                     /label="SeqJJ004"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(6206..6244)
                     /label="KZ791"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    6229..6244
                     /label="USER"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(6243..6279)
                     /label="D243-R2"
                     /note="sequence: GGTTAGGGATAGGCTTTCCtgagccgccagacttcac"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     source          6249..6260
                     /label="source"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    6249..6260
                     /label="SHort linker"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          6254..6287
                     /label="D243-F3"
                     /note="sequence: cggctcaGGAAAGCCTATCCCTAACCCTCTGCTG"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    6261..6302
                     /label="V5 tag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             6303..6305
                     /label="Translation 6303-6305"
                     /translation="G"
     misc_feature    6303..6362
                     /label="yP2A"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             6306..6362
                     /label="P2A"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             6306..6362
                     /label="Translation 6306-6362"
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer          complement(6339..6388)
                     /label="D243-R3"
                     /note="sequence: tcgcccttgctcaccattaagcttgaaggcccgggattttcctCAACATC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             6363..6371
                     /label="Translation 6363-6371"
                     /translation="SSL"
     CDS             6372..7088
                     /label="EGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             6372..7091
                     /label="Translation 6372-7091"
                     /translation="MVSKAEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*"
     misc_feature    complement(6418..6438)
                     /label="EGFP-N primer"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(6418..6438)
                     /label="EGFP-N"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    7025..7046
                     /label="EGFP-C-For"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    7025..7046
                     /label="EGFP-C-For primer"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     complement(7363..7382)
                     /label="T7"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     complement(7363..7383)
                     /label="T7"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     primer_bind     complement(7392..7409)
                     /label="M13-fwd"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     CDS             7480..7547
                     /label="LacZ alpha"
                     /ApEinfo_revcolor="#7ea7d8"
                     /ApEinfo_fwdcolor="#7ea7d8"
     rep_origin      7549..8004
                     /label="M13 origin"
                     /ApEinfo_revcolor="#8493ca"
                     /ApEinfo_fwdcolor="#8493ca"
     rep_origin      7566..7872
                     /label="F1 ori"
                     /ApEinfo_revcolor="#8493ca"
                     /ApEinfo_fwdcolor="#8493ca"
     gene            8305..9908
                     /label="LEU2"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     rep_origin      complement(10542..11052)
                     /label="CEN-ARS pRS"
                     /ApEinfo_revcolor="#f49ac2"
                     /ApEinfo_fwdcolor="#f49ac2"
     CDS             11382..12041
                     /label="AmpR"
                     /ApEinfo_revcolor="#fff79a"
                     /ApEinfo_fwdcolor="#fff79a"
     rep_origin      12139..607
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#8493ca"
                     /ApEinfo_fwdcolor="#8493ca"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtTct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttaGc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcGtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctcag tttatcatta tcaatactcg ccatttcaaa
     1081 gaatacgtaa ataattaata gtagtgattt tcctaacttt atttagtcaa aaaattagcc
     1141 ttttaattct gctgtaaccc gtacatgccc aaaatagggg gcgggttaca cagaatatat
     1201 aacaTcgTag gtgTctgggt gaacagttta ttcctggcat ccactaaata taatggagcc
     1261 cgctttttaa gctggcatcc agaaaaaaaa agaatcccag caccaaaata ttgttttctt
     1321 caccaaccat cagttcatag gtccattctc ttagcgcaac tacagagaac aggggcacaa
     1381 acaggcaaaa aacgggcaca acctcaatgg agtgatgcaa cctgcctgga gtaaatgatg
     1441 acacaaggca attgacccac gcatgtatct atctcatttt cttacacctt ctattacctt
     1501 ctgctctctc tgatttggaa aaagctgaaa aaaaaggttg aaaccagttc cctgaaatta
     1561 ttcccctact tgactaataa gtatataaag acggtaggta ttgattgtaa ttctgtaaat
     1621 ctatttctta aacttcttaa attctacttt tatagttagt ctttttttta gttttaaaac
     1681 accagaactt agtttcgacg gattctagaa ctagtggatc ccccatcaca agtttgtaca
     1741 aaaaagcAGG CTTCGAAGGA GATAACAAAA TGGCCTCCAC TCGTGTCCTC GCCTCTCGCC
     1801 TGGCCTCCCA GATGGCTGCT TCCGCCAAGG TTGCCCGCCC TGCTGTCCGC GTTGCTCAGG
     1861 TCAGCAAGCG CACCATCCAG ACTGGCTCCC CCCTCCAGAC CCTCAAGCGC ACCCAGATGA
     1921 CCTCCATCGT CAACGCCACC ACCCGCCAGG CTTTCCAGAA GCGCGCCTAC TCTTCCtctg
     1981 aggtggagtt ttcccacgag tactggatga gacatgccct gaccctggcc aagagggcac
     2041 gggatgagag ggaggtgcct gtgggagccg tgctggtgct gaacaataga gtgatcggcg
     2101 agggctggaa cagagccatc ggcctgcacg acccaacagc ccatgccgaa attatggccc
     2161 tgagacaggg cggcctggtc atgcagaact acagactgat tgacgccacc ctgtacgtga
     2221 cattcgagcc ttgcgtgatg tgcgccggcg ccatgatcca ctctaggatc ggccgcgtgg
     2281 tgtttggcGT gaggaactca aaaagaggcg ccgcaggctc cctgatgaac gtgctgaact
     2341 accccggcat gaatcaccgc gtcgaaatta ccgagggaat cctggcagat gaatgtgccg
     2401 ccctgctgtg cgatttctat cggatgccta gacaggtgtt caatgctcag aagaaggccc
     2461 agagctccat caactcCgga ggatctagcg gaggatcctc tggcagcgag acaccaggaa
     2521 caagcgagtc agcaacacca gagagcagtg gcggcagcag cggcggcagc agcaagctgg
     2581 agaagtttac aaactgctac tccctgtcta agaccctgag gttcaaggcc atccctgtgg
     2641 gcaagaccca ggagaacatc gacaataagc ggctgctggt ggaggacgag aagagagccg
     2701 aggattataa gggcgtgaag aagctgctgg atcgctacta tctgtctttt atcaacgacg
     2761 tgctgcacag catcaagctg aagaatctga acaattacat cagcctgttc cggaagaaaa
     2821 ccagaaccga gaaggagaat aaggagctgg agaacctgga gatcaatctg cggaaggaga
     2881 tcgccaaggc cttcaagggc aacgagggct acaagtccct gtttaagaag gatatcatcg
     2941 agacaatcct gccagagttc ctggacgata aggacgagat cgccctggtg aacagcttca
     3001 atggctttac cacagccttc accggcttct ttgataacag agagaatatg ttttccgagg
     3061 aggccaagag cacatccatc gccttcaggt gtatcaacga gaatctgacc cgctacatct
     3121 ctaatatgga catcttcgag aaggtggacg ccatctttga taagcacgag gtgcaggaga
     3181 tcaaggagaa gatcctgaac agcgactatg atgtggagga tttctttgag ggcgagttct
     3241 ttaactttgt gctgacacag gagggcatcg acgtgtataa cgccatcatc ggcggcttcg
     3301 tgaccgagag cggcgagaag atcaagggcc tgaacgagta catcaacctg tataatcaga
     3361 aaaccaagca gaagctgcct aagtttaagc cactgtataa gcaggtgctg agcgatcggg
     3421 agtctctgag cttctacggc gagggctata catccgatga ggaggtgctg gaggtgttta
     3481 gaaacaccct gaacaagaac agcgagatct tcagctccat caagaagctg gagaagctgt
     3541 tcaagaattt tgacgagtac tctagcgccg gcatctttgt gaagaacggc cccgccatca
     3601 gcacaatctc caaggatatc ttcggcgagt ggaacgtgat ccgggacaag tggaatgccg
     3661 agtatgacga tatccacctg aagaagaagg ccgtggtgac cgagaagtac gaggacgatc
     3721 ggagaaagtc cttcaagaag atcggctcct tttctctgga gcagctgcag gagtacgccg
     3781 acgccgatct gtctgtggtg gagaagctga aggagatcat catccagaag gtggatgaga
     3841 tctacaaggt gtatggctcc tctgagaagc tgttcgacgc cgattttgtg ctggagaaga
     3901 gcctgaagaa gaacgacgcc gtggtggcca tcatgaagga cctgctggat tctgtgaaga
     3961 gcttcgagaa ttacatcaag gccttctttg gcgagggcaa ggagacaaac agggacgagt
     4021 ccttctatgg cgattttgtg ctggcctacg acatcctgct gaaggtggac cacatctacg
     4081 atgccatccg caattatgtg acccagaagc cctactctaa ggataagttc aagctgtatt
     4141 ttcagaaccc tcagttcatg ggcggctggg acaaggataa ggagacagac tatcgggcca
     4201 ccatcctgag atacggctcc aagtactatc tggccatcat ggataagaag tacgccaagt
     4261 gcctgcagaa gatcgacaag gacgatgtga acggcaatta cgagaagatc aactataagc
     4321 tgctgcccgg ccctaataag atgctgccaa aggtgttctt ttctaagaag tggatggcct
     4381 actataaccc cagcgaggac atccagaaga tctacaagaa tggcacattc aagaagggcg
     4441 atatgtttaa cctgaatgac tgtcacaagc tgatcgactt ctttaaggat agcatctccc
     4501 ggtatccaaa gtggtccaat gcctacgatt tcaacttttc tgagacagag aagtataagg
     4561 acatcgccgg cttttacaga gaggtggagg agcagggcta taaggtgagc ttcgagtctg
     4621 ccagcaagaa ggaggtggat aagctggtgg aggagggcaa gctgtatatg ttccagatct
     4681 ataacaagga cttttccgat aagtctcacg gcacacccaa tctgcacacc atgtacttca
     4741 agctgctgtt tgacgagaac aatcacggac agatcaggct gagcggagga gcagagctgt
     4801 tcatgaggcg cgcctccctg aagaaggagg agctggtggt gcacccagcc aactccccta
     4861 tcgccaacaa gaatccagat aatcccaaga aaaccacaac cctgtcctac gacgtgtata
     4921 aggataagag gttttctgag gaccagtacg agctgcacat cccaatcgcc atcaataagt
     4981 gccccaagaa catcttcaag atcaatacag aggtgcgcgt gctgctgaag cacgacgata
     5041 acccctatgt gatcggcatc gccaggggTg agcgcaatct gctgtatatc gtggtggtgg
     5101 acggcaaggg caacatcgtg gagcagtatt ccctgaacga gatcatcaac aacttcaacg
     5161 gcatcaggat caagacagat taccactctc tgctggacaa gaaggagaag gagaggttcg
     5221 aggcccgcca gaactggacc tccatcgaga atatcaagga gctgaaggcc ggctatatct
     5281 ctcaggtggt gcacaagatc tgcgagctgg tggagaagta cgatgccgtg atcgccctgg
     5341 aggacctgaa ctctggcttt aagaatagcc gcgtgaaggt ggagaagcag gtgtatcaga
     5401 agttcgagaa gatgctgatc gataagctga actacatggt ggacaagaag tctaatcctt
     5461 gtgcaacagg cggcgccctg aagggctatc agatcaccaa taagttcgag agctttaagt
     5521 ccatgtctac ccagaacggc ttcatctttt acatccctgc ctggctgaca tccaagatcg
     5581 atccatctac cggctttgtg aacctgctga aaaccaagta taccagcatc gccgattcca
     5641 agaagttcat cagctccttt gacaggatca tgtacgtgcc cgaggaggat ctgttcgagt
     5701 ttgccctgga ctataagaac ttctctcgca cagacgccga ttacatcaag aagtggaagc
     5761 tgtactccta cggcaaccgg atcagaatct tccggaatcc taagaagaac aacgtgttcg
     5821 actgggagga ggtgtgcctg accagcgcct ataaggagct gttcaacaag tacggcatca
     5881 attatcagca gggcgatatc agagccctgc tgtgcgagca gtccgacaag gccttctact
     5941 ctagctttat ggccctgatg agcctgatgc tgcagatgcg gaacagcatc acaggccgca
     6001 ccgacgtgga ttttctgatc agccctgtga agaactccga cggcatcttc tacgatagcc
     6061 ggaactatga ggcccaggag aatgccatcc tgccaaagaa cgccgacgcc aatggcgcct
     6121 ataacatcgc cagaaaggtg ctgtgggcca tcggccagtt caagaaggcc gaggacgaga
     6181 agctggataa ggtgaagatc gccatctcta acaaggagtg gctggagtac gcccagacca
     6241 gcgtgaagtc tggcggctca GGAAAGCCTA TCCCTAACCC TCTGCTGGGC CTGGACAGCA
     6301 CCggtgcaac aaacttCTCC CTCTTGAAAC AAGCAGGAGA TGTTGaggaa aatcccgggc
     6361 cttcaagctt aatggtgagc aaggCcgagg agctgttcac cggggtggtg cccatcctgg
     6421 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     6481 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     6541 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     6601 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     6661 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     6721 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     6781 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     6841 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     6901 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     6961 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     7021 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     7081 tgtacaagta actcgagtca tgtaattagt tatgtcacgc ttacattcac gccctccccc
     7141 cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta
     7201 tttttttata gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt
     7261 tctgtacaga cgcgtgtacg catgtaacat tatactgaaa accttgcttg agaaggtttt
     7321 gggacgctcg aaggctttaa tttgcggccg gtacccaatt cgccctatag tgagtcgtat
     7381 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     7441 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     7501 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggac gcgccctgta
     7561 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     7621 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     7681 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     7741 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     7801 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     7861 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     7921 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     7981 acaaaatatt aacgCttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg
     8041 tatttcacac cgcatatcga Ccggtcgagg agaacttcta gtatatcTac atacctaata
     8101 ttattgcctt attaaaaatg gaatcccaac aattacatca aaatccacat tctcttcaaa
     8161 atcaattgtc ctgtacttcc ttgttcatgt gtgttcaaaa acgttatatt tataggataa
     8221 ttatactcta tttctcaaca agtaattggt tgtttggccg agcggtctaa ggcgcctgat
     8281 tcaagaaata tcttgaccgc agttaactgt gggaatactc aggtatcgta agatgcaaga
     8341 gttcgaatct cttagcaacc attatttttt tcctcaacat aacgagaaca cacaggggcg
     8401 ctatcgcaca gaatcaaatt cgatgactgg aaattttttg ttaatttcag aggtcgcctg
     8461 acgcatatac ctttttcaac tgaaaaattg ggagaaaaag gaaaggtgag agCgccggaa
     8521 ccggcttttc atatagaata gagaagcgtt catgactaaa tgcttgcatc acaatacttg
     8581 aagttgacaa tattatttaa ggacctattg ttttttccaa taggtggtta gcaatcgtct
     8641 tactttctaa cttttcttac cttttacatt tcagcaATat atatatatat atttcaagga
     8701 tataccattc taatgtctgc ccctaagaag atcgtcgttt tgccaggtga ccacgttggt
     8761 caagaaatca cagccgaagc cattaaggtt cttaaagcta tttctgatgt tcgttccaat
     8821 gtcaagttcg atttcgaaaa tcatttaatt ggtggtgctg ctatcgatgc tacaggtgtT
     8881 ccacttccag atgaggcgct ggaagcctcc aagaaggCtg atgccgtttt gttaggtgct
     8941 gtgggtggtc ctaaatgggg taccggtagt gttagacctg aacaaggttt actaaaaatc
     9001 cgtaaagaac ttcaattgta cgccaactta agaccatgta actttgcatc cgactctctt
     9061 ttagacttat ctccaatcaa gccacaattt gctaaaggta ctgacttcgt tgttgtcaga
     9121 gaattagtgg gaggtattta ctttggtaag agaaaggaag acgatggtga tggtgtcgct
     9181 tgggatagtg aacaatacac cgttccagaa gtgcaaagaa tcacaagaat ggccgctttc
     9241 atggccctac aacatgagcc accattgcct atttggtcct tggataaagc taatgttttg
     9301 gcctcttcaa gattatggag aaaaactgtg gaggaaacca tcaagaacga attccctaca
     9361 ttgaaggttc aacatcaatt gattgattct gccgccatga tcctagttaa gaacccaacc
     9421 cacctaaatg gtattataat caccagcaac atgtttggtg atatcatctc cgatgaagcc
     9481 tccgttatcc caggttcctt gggtttgttg ccatctgcgt ccttggcctc tttgccagac
     9541 aagaacaccg catttggttt gtacgaacca tgccacggtt ctgctccaga tttgccaaag
     9601 aataaggtCA accctatcgc cactatcttg tctgctgcaa tgatgttgaa attgtcattg
     9661 aacttgcctg aagaaggtaa ggccattgaa gatgcagtta aaaaggtttt ggatgcaggt
     9721 atcagaactg gtgatttagg tggttccaac agtaccaccg aagtcggtga tgctgtcgcc
     9781 gaagaagtta agaaaatcct tgcttaaaaa gattctcttt ttttatgata tttgtacata
     9841 aactttataa atgaaattca taatagaaac gacacgaaat tacaaaatgg aatatgttca
     9901 tagggtagac gaaactatat acgcaatcta catacattta tcaagaagga gaaaaaggag
     9961 gatgtaaagg aatacaggta agcaaattga tactaatggc tcaacgtgat aaggaaaaag
    10021 aattgcactt taacattaat attgacaagg aggagggcac cacacaaaaa gttaggtgta
    10081 acagaaaatc atgaaactaT gattcctaat ttATatattg gaggattttc tctaaaaaaa
    10141 aaaaatacaa caaataaaaa acactcaatg acctgaccat ttgatggagt ttaagtcaat
    10201 accttcttga accatttccc ataatggtga aagttccctc aagaatttta ctctgtcaga
    10261 aacggccttA acgacgtagt cgatatggtg cactctcagt acaatctgct ctgatgccgc
    10321 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
    10381 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
    10441 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
    10501 ataggttaat gtcatgataa taatggtttc ttaggacgga tcgcttgcct gtaacttaca
    10561 cgcgcctcgt atcttttaat gatggaataa tttgggaatt tactctgtgt ttatttattt
    10621 ttatgttttg tatttggatt ttagaaagta aataaagaag gtagaagagt tacggaatga
    10681 agaaaaaaaa ataaacaaag gtttaaaaaa tttcaacaaa aagcgtactt tacatatata
    10741 tttattagac aagaaaagca gattaaatag atatacattc gattaacgat aagtaaaatg
    10801 taaaatcaca ggattttcgt gtgtggtctt ctacacagac aagatgaaac aattcggcat
    10861 taatacctga gagcaggaag agcaagataa aaggtagtat ttgttggcga tccccctaga
    10921 gtcttttaca tcttcggaaa acaaaaacta ttttttcttt aatttctttt tttactttct
    10981 atttttaatt tatatattta tattaaaaaa tttaaattat aattattttt atagcacgtg
    11041 atgaaaagga cccaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
    11101 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
    11161 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
    11221 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
    11281 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
    11341 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
    11401 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
    11461 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
    11521 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
    11581 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
    11641 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
    11701 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
    11761 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
    11821 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
    11881 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
    11941 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
    12001 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
    12061 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
    12121 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
    12181 cgtcagaccc cgtagaaaag atcaaaggat cttc
//