LOCUS       PSY190-pAG415GPD-NLS-A 12569 bp ds-DNA     circular     19-AUG-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      complement(73..583)
                     /label="CEN-ARS pRS"
                     /ApEinfo_revcolor="#f49ac2"
                     /ApEinfo_fwdcolor="#f49ac2"
     CDS             913..1572
                     /label="AmpR"
                     /ApEinfo_revcolor="#fff79a"
                     /ApEinfo_fwdcolor="#fff79a"
     rep_origin      1670..2352
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#8493ca"
                     /ApEinfo_fwdcolor="#8493ca"
     misc_binding    2692..2714
                     /label="LacO"
                     /ApEinfo_revcolor="#7ea7d8"
                     /ApEinfo_fwdcolor="#7ea7d8"
     primer_bind     2720..2740
                     /label="M13-rev"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     promoter        2757..2775
                     /label="T3 promoter"
                     /ApEinfo_revcolor="#8882be"
                     /ApEinfo_fwdcolor="#8882be"
     primer_bind     2758..2777
                     /label="T3"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     misc_feature    2794..3443
                     /label="GPD_promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          3318..3367
                     /label="SY32-seq-F1-GPDF"
                     /note="sequence: GACTAATAAGTATATAAAGACGGTAGGTATTGATTGTAATTCTGTAAATC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          3470..3500
                     /label="GUF"
                     /note="sequence: GTACAAAAAAGCAGGCTTCGAAGGAGATAAC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3474..3498
                     /label="attB1_invitrogen"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_recomb     3475..3493
                     /label="attR1"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_recomb     3475..3493
                     /label="attB1"
                     /ApEinfo_revcolor="#bc8dbf"
                     /ApEinfo_fwdcolor="#bc8dbf"
     misc_feature    3478..3497
                     /label="ATTB1 primer"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_recomb     3479..3493
                     /label="attB1_short"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          3504..3526
                     /label="D158-F1"
                     /note="sequence: atgaaacggacagccgacggaag"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    3504..4154
                     /label="ABE8a_bef"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             3504..4154
                     /label="Translation 3504-4154"
                     /translation="MKRTADGSEFESPKKKRKVSEVEFSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWNRAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTFEPCVMCAGAMIHSRIGRVVFGVRNSKRGAAGSLMNVLNYPGMNHRVEITEGILADECAALLCDFYRMPRQVFNAQKKAQSSINSGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     CDS             3540..3560
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             4155..4178
                     /label="Cas9(D10A)"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     primer          4155..4178
                     /label="D159-F1"
                     /note="sequence: gacaagaagtacagcatcggcctg"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(4155..4178)
                     /label="D158-R1"
                     /note="sequence: caggccgatgctgtacttcttgtc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             4155..8255
                     /label="Cas9-(JHD10A)"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             4155..8403
                     /label="Translation 4155-8403"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDEGADPKKKRKVDPKKKRKVDPKKKRKVDYKDHDGDYKDHDIDYKDDDDK"
     primer          complement(4160..4181)
                     /label="D83-R1"
                     /note="sequence: GGCcaggccgatgctgtacttc"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          4160..4207
                     /label="D82-F1"
                     /note="sequence: gaagtacagcatcggcctgGCCattggtactaactctgtgggttgggc"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    4179..4181
                     /label="D10A"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(4487..4521)
                     /label="JH-seq-R1"
                     /note="sequence: caactatattcccaaagattggatgtctctcatgc"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          4920..4961
                     /label="119-seq-F2"
                     /note="sequence: gatttagctgaagatgctaaattacaattatcaaaagacacc"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          5586..5616
                     /label="119-seq-F3"
                     /note="sequence: gaagaggttgttgataaaggagcatcagcac"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          6292..6324
                     /label="119-seq-F4"
                     /note="sequence: gtgggcaaggtgattcattacatgaacatattg"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          6995..7040
                     /label="119-seq-F5"
                     /note="sequence: gttaatacgtgaggttaaagtcattactttaaaatcaaaacttgtc"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          7658..7697
                     /label="119-seq-F6"
                     /note="sequence: ggaaagaagttcctttgaaaagaaccctattgattttttg"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          8082..8115
                     /label="D119-F1"
                     /note="sequence: cacttatttacccttaccaatttaggagcacctg"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          8256..8290
                     /label="D86-F1"
                     /note="sequence: gaaggggctgatcctaagaagaaaagaaaagttga"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             8268..8288
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             8292..8312
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             8316..8336
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             8337..8403
                     /label="3xFLAG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             8404..8417
                     /label="Translation 8404-8417"
                     /translation="TQLS"
     misc_feature    complement(8404..8427)
                     /label="ATTB2 primer"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    8404..8428
                     /label="attB2_invitrogen"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_recomb     complement(8407..8427)
                     /label="attB2"
                     /ApEinfo_revcolor="#bc8dbf"
                     /ApEinfo_fwdcolor="#bc8dbf"
     misc_feature    complement(8407..8427)
                     /label="attR2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             8418..8459
                     /label="Translation 8418-8459"
                     /translation="YKVVMGCRNSISSL"
     CDS             8460..9176
                     /label="EGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             8460..9179
                     /label="Translation 8460-9179"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*"
     primer          8473..8492
                     /label="SY28-seq-F3"
                     /note="sequence: gcgaggagctgttcaccggg"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(8506..8526)
                     /label="EGFP-N primer"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(8506..8526)
                     /label="EGFP-N"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    9113..9134
                     /label="EGFP-C-For"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    9113..9134
                     /label="EGFP-C-For primer"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     complement(9451..9470)
                     /label="T7"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     complement(9451..9471)
                     /label="T7"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     primer_bind     complement(9480..9497)
                     /label="M13-fwd"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     CDS             9568..9636
                     /label="LacZ alpha"
                     /ApEinfo_revcolor="#7ea7d8"
                     /ApEinfo_fwdcolor="#7ea7d8"
     rep_origin      9641..10096
                     /label="M13 origin"
                     /ApEinfo_revcolor="#8493ca"
                     /ApEinfo_fwdcolor="#8493ca"
     rep_origin      9658..9964
                     /label="F1 ori"
                     /ApEinfo_revcolor="#8493ca"
                     /ApEinfo_fwdcolor="#8493ca"
     gene            10396..12008
                     /label="LEU2"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata
      121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt
      181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa
      241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata
      301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct
      361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata
      421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact
      481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa
      541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg
      601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1861 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     2521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     2581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttacctcact
     2641 cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg tggaattgtg
     2701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     2761 aaccctcact aaagggaaca aaagctggag ctcagtttat cattatcaat actcgccatt
     2821 tcaaagaata cgtaaataat taatagtagt gattttccta actttattta gtcaaaaaat
     2881 tagcctttta attctgctgt aacccgtaca tgcccaaaat agggggcggg ttacacagaa
     2941 tatataacac cgaaggtgcc tgggtgaaca ggtttattcc tggcatccac taaatataat
     3001 ggagcccgct ttttaagctg gcatccagaa aaaaaaagaa tcccagcacc aaaatattgt
     3061 tttcttcacc aaccatcagt tcataggtcc attctcttag cgcaactaca gagaacaggg
     3121 gcacaaacag gcaaaaaacg ggcacaacct caatggagtg atgcaacctg cctggagtaa
     3181 atgatgacac aaggcaattg acccacgcat gtatctatct cattttctta caccttctat
     3241 taccttctgc tctctctgat ttggaaaaag ctgaaaaaaa aggttgaaac cagttccctg
     3301 aaattattcc cctacttgac taataagtat ataaagacgg taggtattga ttgtaattct
     3361 gtaaatctat ttcttaaact tcttaaattc tacttttata gttagtcttt tttttagttt
     3421 taaaacacca gaacttagtt tcgacggatt ctagaactag tggatccccc atcacaagtt
     3481 tgtacaaaaa agcAGGCTAC AAAatgaaac ggacagccga cggaagcgag ttcgagtcac
     3541 caaagaagaa gcggaaagtc tctgaggtgg agttttccca cgagtactgg atgagacatg
     3601 ccctgaccct ggccaagagg gcacgggatg agagggaggt gcctgtggga gccgtgctgg
     3661 tgctgaacaa tagagtgatc ggcgagggct ggaacagagc catcggcctg cacgacccaa
     3721 cagcccatgc cgaaattatg gccctgagac agggcggcct ggtcatgcag aactacagac
     3781 tgattgacgc caccctgtac gtgacattcg agccttgcgt gatgtgcgcc ggcgccatga
     3841 tccactctag gatcggccgc gtggtgtttg gcgtgaggaa ctcaaaaaga ggcgccgcag
     3901 gctccctgat gaacgtgctg aactaccccg gcatgaatca ccgcgtcgaa attaccgagg
     3961 gaatcctggc agatgaatgt gccgccctgc tgtgcgattt ctatcggatg cctagacagg
     4021 tgttcaatgc tcagaagaag gcccagagct ccatcaactc cggaggatct agcggaggct
     4081 cctctggctc tgagacacct ggcacaagcg agagcgcaac acctgaaagc agcgggggca
     4141 gcagcggggg gtcagacaag aagtacagca tcggcctgGC Cattggtact aactctgtgg
     4201 gttgggcagt tatcactgac gaatataaag ttccatcaaa gaaatttaag gtgttaggta
     4261 acactgacag acactcaata aaaaagaatc ttatcggtgc tcttttgttc gactccggtg
     4321 aaactgccga ggctacacgt ttaaaaagaa cagcaagaag aagatatacc cgtagaaaaa
     4381 atagaatatg ttatttacaa gaaatctttt ctaatgaaat ggctaaagtt gatgattcct
     4441 ttttccatag attggaagag tcatttttgg ttgaagaaga caaaaagcat gagagacatc
     4501 caatctttgg gaatatagtt gatgaagtgg cttaccatga aaaatatcct accatttatc
     4561 atttaagaaa gaaattggta gattcaactg ataaagctga ccttagatta atctatttag
     4621 cacttgccca tatgattaaa tttagaggtc attttttgat tgaaggtgat ttgaacccag
     4681 ataattctga cgtggataaa ttatttattc aattagtcca aacctacaac caattatttg
     4741 aggaaaatcc aattaatgct agtggtgtcg atgccaaagc tatattatca gccagattat
     4801 caaaatctag acgtttggaa aatttgattg cccaattgcc aggagaaaaa aagaatggat
     4861 tatttggaaa cttgatcgca ttatcattgg gtttgacacc aaattttaaa tctaattttg
     4921 atttagctga agatgctaaa ttacaattat caaaagacac ctatgacgac gatttggaca
     4981 atttacttgc tcaaattggt gatcaatatg cagatttgtt cttagctgct aaaaacttat
     5041 ctgatgctat tttgttgtct gatattttga gagtgaacac agaaataacc aaagctccat
     5101 tatcagcatc tatgatcaaa cgttatgatg aacaccatca ggatttgact ttattgaaag
     5161 ctttggtgag acaacaattg ccagagaagt ataaagaaat ctttttcgat caatctaaaa
     5221 acgggtatgc aggttatatt gatgggggtg cctcccaaga ggaattttac aaatttataa
     5281 aacctatttt agaaaagatg gatgggactg aggaactttt ggtcaaattg aacagagaag
     5341 atttgttacg taaacagaga acttttgata atggtagtat acctcaccaa attcatttgg
     5401 gtgagttgca tgcaatttta agaagacaag aagattttta tccattttta aaagataata
     5461 gagaaaaaat cgagaaaatt ttaaccttta gaattccata ctatgttggg cctttggcta
     5521 gaggtaattc aagatttgcc tggatgacac gtaaatcaga agaaactatt accccttgga
     5581 attttgaaga ggttgttgat aaaggagcat cagcacagag ttttattgaa agaatgacca
     5641 atttcgataa aaacttacca aatgaaaaag ttttaccaaa acattccttg ttatacgaat
     5701 attttactgt ttacaatgaa cttacaaagg ttaaatatgt tactgaaggt atgcgtaagc
     5761 cagccttttt atctggagaa cagaaaaagg caatagttga tttattgttt aaaacaaata
     5821 gaaaagttac tgttaaacaa ttaaaagaag attactttaa gaaaattgaa tgttttgatt
     5881 cagttgaaat cagtggtgtt gaagacagat ttaatgctag tttaggaact taccatgatt
     5941 tacttaaaat tatcaaagat aaagatttct tggataacga agaaaatgaa gacattttag
     6001 aagacattgt tttaacctta actttattcg aagatagaga gatgattgaa gaacgtttga
     6061 agacttatgc acatttgttt gacgataaag tgatgaaaca gttgaaaaga agacgttata
     6121 ctggatgggg tagattgtct cgtaaattga tcaatggaat tagagataaa caaagtggta
     6181 aaactatctt ggactttttg aaatctgacg gatttgctaa tagaaatttc atgcaattga
     6241 tccacgacga tagtttgaca tttaaagaag acatccaaaa ggcccaagtg agtgggcaag
     6301 gtgattcatt acatgaacat attgcaaatt tagccggatc tcctgctatt aagaaaggga
     6361 tattacaaac tgttaaagtt gtggatgaat tagtgaaagt aatgggaaga cataaacctg
     6421 aaaacattgt cattgagatg gcaagagaaa atcaaactac acaaaaagga cagaaaaata
     6481 gtagagaacg tatgaaaaga atagaagagg gtattaaaga attgggtagt caaatattga
     6541 aagaacaccc agtggaaaat acccagttgc aaaatgaaaa attatatctt tactaccttc
     6601 aaaatggacg tgatatgtat gttgatcagg aattagatat aaatagactt tcagattatg
     6661 atgtagatca tatagttcca caatctttct tgaaagatga ttccatagac aataaagtat
     6721 taactagaag tgataaaaat agaggtaaaa gtgataatgt cccaagtgag gaagtcgtca
     6781 aaaagatgaa aaattactgg cgtcaacttt tgaatgctaa attaattact caaagaaaat
     6841 ttgataattt gactaaagca gaaagaggtg ggctttctga attagataaa gccgggttca
     6901 ttaaaagaca attggtcgaa actagacaaa ttactaaaca tgttgcccaa attttagatt
     6961 cccgtatgaa cactaagtat gacgaaaatg ataagttaat acgtgaggtt aaagtcatta
     7021 ctttaaaatc aaaacttgtc tctgatttca gaaaggattt ccaattctat aaagttagag
     7081 aaattaataa ttatcatcat gctcatgatg catatttgaa tgctgtagtt ggaactgctt
     7141 taatcaagaa ataccctaaa ttagaatctg aatttgtata tggtgattac aaagtctatg
     7201 atgttagaaa gatgattgct aaatcagaac aagaaattgg taaagctaca gctaaatact
     7261 tcttttactc taacattatg aatttcttta aaacagaaat tactttggca aacggtgaaa
     7321 ttagaaaaag acctcttatt gaaacaaatg gtgagactgg agagatagtt tgggacaaag
     7381 ggcgtgattt cgctactgtt agaaaagttt tatcaatgcc acaagttaac attgtaaaga
     7441 aaacagaggt tcaaactggt ggtttctcaa aagaaagtat tttgcctaaa agaaatagtg
     7501 ataaattgat tgccagaaaa aaggattggg atccaaagaa atatggtggt ttcgactcac
     7561 caaccgtagc ctattctgtt ttggttgtgg caaaggttga aaagggtaaa agtaaaaagc
     7621 ttaaatcagt aaaagaactt ttgggtatta caataatgga aagaagttcc tttgaaaaga
     7681 accctattga ttttttggaa gctaaaggtt ataaggaagt aaagaaggac ttaataatca
     7741 aattgcctaa atattcttta tttgaattag aaaatgggag aaaaagaatg ttggcttctg
     7801 ctggagaatt gcaaaagggt aatgaattag cattgccttc caaatatgtt aacttcttgt
     7861 atttagcttc acactatgaa aagttgaaag ggtcaccaga agataacgag caaaaacaat
     7921 tatttgttga acaacacaaa cactacttag atgagattat agaacaaatt agtgaattca
     7981 gtaaaagagt gatattagct gatgcaaatt tagataaagt tttgtcagcc tataacaaac
     8041 atagagataa gccaattaga gaacaagcag aaaacattat tcacttattt acccttacca
     8101 atttaggagc acctgctgct ttcaagtatt ttgatacaac aattgatcgt aaaagatata
     8161 cctcaacaaa agaagtctta gacgccacct taattcatca atcaatcact ggattgtatg
     8221 agacaagaat tgatttgtct caattgggtg gtgatgaagg ggctgatcct aagaagaaaa
     8281 gaaaagttga tccaaagaaa aagcgtaagg tggatcctaa gaaaaagaga aaggttgact
     8341 acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat gacgatgaca
     8401 aGgACCcagc tttcttgtac aaagtggtga tgggctgcag gaattcgata tcaagcttaa
     8461 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     8521 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     8581 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     8641 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     8701 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     8761 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     8821 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     8881 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     8941 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     9001 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     9061 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     9121 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaac
     9181 tcgagtcatg taattagtta tgtcacgctt acattcacgc cctcccccca catccgctct
     9241 aaccgaaaag gaaggagtta gacaacctga agtctaggtc cctatttatt tttttatagt
     9301 tatgttagta ttaagaacgt tatttatatt tcaaattttt cttttttttc tgtacagacg
     9361 cgtgtacgca tgtaacatta tactgaaaac cttgcttgag aaggttttgg gacgctcgaa
     9421 ggctttaatt tgcggccggt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     9481 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     9541 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     9601 ccttcccaac agttgcgcag cctgaatggc gaatggcgcg acgcgccctg tagcggcgca
     9661 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     9721 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     9781 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     9841 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     9901 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     9961 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
    10021 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
    10081 ttaacgttta caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
    10141 accgcatatc gacggtcgag gagaacttct agtatatcca catacctaat attattgcct
    10201 tattaaaaat ggaatcccaa caattacatc aaaatccaca ttctcttcaa aatcaattgt
    10261 cctgtacttc cttgttcatg tgtgttcaaa aacgttatat ttataggata attatactct
    10321 atttctcaac aagtaattgg ttgtttggcc gagcggtcta aggcgcctga ttcaagaaat
    10381 atcttgaccg cagttaactg tgggaatact caggtatcgt aagatgcaag agttcgaatc
    10441 tcttagcaac cattattttt ttcctcaaca taacgagaac acacaggggc gctatcgcac
    10501 agaatcaaat tcgatgactg gaaatttttt gttaatttca gaggtcgcct gacgcatata
    10561 cctttttcaa ctgaaaaatt gggagaaaaa ggaaaggtga gaggccggaa ccggcttttc
    10621 atatagaata gagaagcgtt catgactaaa tgcttgcatc acaatacttg aagttgacaa
    10681 tattatttaa ggacctattg ttttttccaa taggtggtta gcaatcgtct tactttctaa
    10741 cttttcttac cttttacatt tcagcaatat atatatatat ttcaaggata taccattcta
    10801 atgtctgccc ctatgtctgc ccctaagaag atcgtcgttt tgccaggtga ccacgttggt
    10861 caagaaatca cagccgaagc cattaaggtt cttaaagcta tttctgatgt tcgttccaat
    10921 gtcaagttcg atttcgaaaa tcatttaatt ggtggtgctg ctatcgatgc tacaggtgtc
    10981 ccacttccag atgaggcgct ggaagcctcc aagaaggttg atgccgtttt gttaggtgct
    11041 gtgggtggtc ctaaatgggg taccggtagt gttagacctg aacaaggttt actaaaaatc
    11101 cgtaaagaac ttcaattgta cgccaactta agaccatgta actttgcatc cgactctctt
    11161 ttagacttat ctccaatcaa gccacaattt gctaaaggta ctgacttcgt tgttgtcaga
    11221 gaattagtgg gaggtattta ctttggtaag agaaaggaag acgatggtga tggtgtcgct
    11281 tgggatagtg aacaatacac cgttccagaa gtgcaaagaa tcacaagaat ggccgctttc
    11341 atggccctac aacatgagcc accattgcct atttggtcct tggataaagc taatgttttg
    11401 gcctcttcaa gattatggag aaaaactgtg gaggaaacca tcaagaacga attccctaca
    11461 ttgaaggttc aacatcaatt gattgattct gccgccatga tcctagttaa gaacccaacc
    11521 cacctaaatg gtattataat caccagcaac atgtttggtg atatcatctc cgatgaagcc
    11581 tccgttatcc caggttcctt gggtttgttg ccatctgcgt ccttggcctc tttgccagac
    11641 aagaacaccg catttggttt gtacgaacca tgccacggtt ctgctccaga tttgccaaag
    11701 aataaggttg accctatcgc cactatcttg tctgctgcaa tgatgttgaa attgtcattg
    11761 aacttgcctg aagaaggtaa ggccattgaa gatgcagtta aaaaggtttt ggatgcaggt
    11821 atcagaactg gtgatttagg tggttccaac agtaccaccg aagtcggtga tgctgtcgcc
    11881 gaagaagtta agaaaatcct tgcttaaaaa gattctcttt ttttatgata tttgtacata
    11941 aactttataa atgaaattca taatagaaac gacacgaaat tacaaaatgg aatatgttca
    12001 tagggtagac gaaactatat acgcaatcta catacattta tcaagaagga gaaaaaggag
    12061 gatagtaaag gaatacaggt aagcaaattg atactaatgg ctcaacgtga taaggaaaaa
    12121 gaattgcact ttaacattaa tattgacaag gaggagggca ccacacaaaa agttaggtgt
    12181 aacagaaaat catgaaacta cgattcctaa tttgatattg gaggattttc tctaaaaaaa
    12241 aaaaaataca acaaataaaa aacactcaat gacctgacca tttgatggag tttaagtcaa
    12301 taccttcttg aaccatttcc cataatggtg aaagttccct caagaatttt actctgtcag
    12361 aaacggcctt acgacgtagt cgatatggtg cactctcagt acaatctgct ctgatgccgc
    12421 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
    12481 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
    12541 gttttcaccg tcatcaccga aacgcgcga
//