LOCUS       PSY328-pAG415GPD-SU9MT 13897 bp ds-DNA     circular     16-OCT-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          67..96
                     /label="VSY3-seq-F12"
                     /note="sequence: ggtttgtttgccggatcaagagctaccaac"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          828..850
                     /label="VSY3-seq-F13"
                     /note="sequence: ggcacgacaggtttcccgactgg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_binding    947..969
                     /label="LacO"
                     /ApEinfo_revcolor="#7ea7d8"
                     /ApEinfo_fwdcolor="#7ea7d8"
     primer_bind     975..995
                     /label="M13-rev"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     promoter        1012..1030
                     /label="T3 promoter"
                     /ApEinfo_revcolor="#8882be"
                     /ApEinfo_fwdcolor="#8882be"
     primer_bind     1013..1032
                     /label="T3"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     primer          1046..1086
                     /label="D142-F1"
                     /note="sequence: ctcagtttatcattatcaatactcgccatttcaaagaatac"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    1049..1697
                     /label="GPD_promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          1572..1621
                     /label="SY32-seq-F1-GPDF"
                     /note="sequence: GACTAATAAGTATATAAAGACGGTAGGTATTGATTGTAATTCTGTAAATC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          1683..1749
                     /label="D2201-a"
                     /note="sequence: cagaacttagtttcgacggattctagaactagtggatcccccatcacaagtttgtacaaaaaagcAG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          1687..1766
                     /label="D205-Fa"
                     /note="sequence: acttagtttcgacggattctagaactagtggatcccccatcacaagtttgtacaaaaaagcAGGCTTCGAAGGAGATAAC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          1690..1739
                     /label="E1-F1"
                     /note="sequence: tagtttcgacggattctagaactagtggatcccccatcacaagtttgtac"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          1692..1716
                     /label="D204-F2"
                     /note="sequence: gtttcgacggattctagaactagtg"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1720..1761
                     /label="D206-FF1"
                     /note="sequence: cccccatcacaagTTTGTACAAAAAAGCAGGCTTCGAAGGAG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          1721..1790
                     /label="D205-F1"
                     /note="sequence: ccccatcacaagtttgtacaaaaaagcAGGCTTCGAAGGAGATAACAAAATGGCCTCCACTCGTGTCCTC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1728..1752
                     /label="attB1_invitrogen"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_recomb     1729..1747
                     /label="attB1"
                     /ApEinfo_revcolor="#bc8dbf"
                     /ApEinfo_fwdcolor="#bc8dbf"
     misc_recomb     1729..1747
                     /label="attR1"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1732..1751
                     /label="ATTB1 primer"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_recomb     1733..1747
                     /label="attB1_short"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1736..1766
                     /label="GUF"
                     /note="sequence: GTACAAAAAAGCAGGCTTCGAAGGAGATAAC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          1770..1849
                     /label="D129-Fa"
                     /note="sequence: ATGGCCTCCACTCGTGTCCTCGCCTCTCGCCTGGCCTCCCAGATGGCTGCTTCCGCCAAGGTTGCCCGCCCTGCTGTCCG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1770..1976
                     /label="SU9MTS"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             1770..8774
                     /label="Translation 1770-8774"
                     /translation="MASTRVLASRLASQMAASAKVARPAVRVAQVSKRTIQTGSPLQTLKRTQMTSIVNATTRQAFQKRAYSSSEVEFSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWNRAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTFEPCVMCAGAMIHSRIGRVVFGWRNSKRGAAGSLMNVLNYPGMNHRVEITEGILADECAALLCDFYRMPRQVFNAQKKAQSSINSGGSSGGSSGSETPGTSESATPESSGGSSGGSDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDSGGSFKRYLGSSTSSRRFTSILEEKAFQVTTYSRPEDLCITRGKNAKLYDDVNGKEYIDFTAGIAVTALGHANPKVAEILHHQANKLVHSSNLYFTKECLDLSEKIVEKTKQFGGQHDASRVFLCNSGTEANEAALKFAKKHGIMKNPSKQGIVAFENSFHGRTMGALSVTWNSKYRTPFGDLVPHVSFLNLNDEMTKLQSYIETKKDEIAGLIVEPIQGEGGVFPVEVEKLTGLKKICQDNDVIVIHDEIQCGLGRSGKLWAHAYLPSEAHPDIFTSAKALGNGFPIAATIVNEKVNNALRVGDHGTTYGGNPLACSVSNYVLDTIADEAFLKQVSKKSDILQKRLREIQAKYPNQIKTIRGKGLMLGAEFVEPPTEVIKKARELGLLIITAGKSTVRFVPALTIEDELIEEGMDAFEKAIEAVYAGKPIPNPLLGLDSTGATNFSLLKQAGDVEENPGPMVSKAEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*"
     primer          complement(1833..1912)
                     /label="D129-Fb"
                     /note="sequence: GTGCGCTTGAGGGTCTGGAGGGGGGAGCCAGTCTGGATGGTGCGCTTGCTGACCTGAGCAACGCGGACAGCAGGGCGGGC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(1893..1972)
                     /label="D129-Rd"
                     /note="sequence: GAGTAGGCGCGCTTCTGGAAAGCCTGGCGGGTGGTGGCGTTGACGATGGAGGTCATCTGGGTGCGCTTGAGGGTCTGGAG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          1953..2003
                     /label="D2204-F"
                     /note="sequence: TTCCAGAAGCGCGCCTACTCTTCCtctgaggtggagttttcccacgagtac"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1977..2570
                     /label="ABE8e_bef"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             1977..7994
                     /label="Translation 1977-7994"
                     /translation="SEVEFSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWNRAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTFEPCVMCAGAMIHSRIGRVVFGWRNSKRGAAGSLMNVLNYPGMNHRVEITEGILADECAALLCDFYRMPRQVFNAQKKAQSSINSGGSSGGSSGSETPGTSESATPESSGGSSGGSDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDSGGSFKRYLGSSTSSRRFTSILEEKAFQVTTYSRPEDLCITRGKNAKLYDDVNGKEYIDFTAGIAVTALGHANPKVAEILHHQANKLVHSSNLYFTKECLDLSEKIVEKTKQFGGQHDASRVFLCNSGTEANEAALKFAKKHGIMKNPSKQGIVAFENSFHGRTMGALSVTWNSKYRTPFGDLVPHVSFLNLNDEMTKLQSYIETKKDEIAGLIVEPIQGEGGVFPVEVEKLTGLKKICQDNDVIVIHDEIQCGLGRSGKLWAHAYLPSEAHPDIFTSAKALGNGFPIAATIVNEKVNNALRVGDHGTTYGGNPLACSVSNYVLDTIADEAFLKQVSKKSDILQKRLREIQAKYPNQIKTIRGKGLMLGAEFVEPPTEVIKKARELGLLIITAGKSTVRFVPALTIEDELIEEGMDAFEKAIEAVYAGKPIPNPLLGLDST"
     misc_feature    2273..2288
                     /label="reverse"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(2273..2307)
                     /label="D204-R2"
                     /note="sequence: ctctttttgagttcctCCAgccaaacaccacgcgg"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2280..2307
                     /label="anneal"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          2280..2311
                     /label="D204-F1"
                     /note="sequence: gtgtttggcTGGaggaactcaaaaagaggcgc"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2292..2311
                     /label="forward"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          2292..2311
                     /label="204seq-F1"
                     /note="sequence: aggaactcaaaaagaggcgc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             2571..2594
                     /label="Cas9(D10A)"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     primer          2571..2594
                     /label="D159-F1"
                     /note="sequence: gacaagaagtacagcatcggcctg"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(2571..2594)
                     /label="D158-R1"
                     /note="sequence: caggccgatgctgtacttcttgtc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2571..6671
                     /label="ABE"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             2571..7994
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDSGGSFKRYLGSSTSSRRFTSILEEKAFQVTTYSRPEDLCITRGKNAKLYDDVNGKEYIDFTAGIAVTALGHANPKVAEILHHQANKLVHSSNLYFTKECLDLSEKIVEKTKQFGGQHDASRVFLCNSGTEANEAALKFAKKHGIMKNPSKQGIVAFENSFHGRTMGALSVTWNSKYRTPFGDLVPHVSFLNLNDEMTKLQSYIETKKDEIAGLIVEPIQGEGGVFPVEVEKLTGLKKICQDNDVIVIHDEIQCGLGRSGKLWAHAYLPSEAHPDIFTSAKALGNGFPIAATIVNEKVNNALRVGDHGTTYGGNPLACSVSNYVLDTIADEAFLKQVSKKSDILQKRLREIQAKYPNQIKTIRGKGLMLGAEFVEPPTEVIKKARELGLLIITAGKSTVRFVPALTIEDELIEEGMDAFEKAIEAVYAGKPIPNPLLGLDST"
     primer          complement(2576..2597)
                     /label="D83-R1"
                     /note="sequence: GGCcaggccgatgctgtacttc"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             2595..2597
                     /label="Cas9(D10A)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             2598..6671
                     /label="Cas9(D10A)"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     primer          2800..2828
                     /label="ABE-s2"
                     /note="sequence: ggatctgctatctgcaagagatcttcagc"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(2817..2839)
                     /label="D201-R2"
                     /note="sequence: gccatctcgttgctgaagatctc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(2819..2839)
                     /label="E1-R1"
                     /note="sequence: gccatctcgttgctgaagatc"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(2819..2843)
                     /label="D201-R"
                     /note="sequence: cttggccatctcgttgctgaagatc"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          3588..3614
                     /label="ABE-s3"
                     /note="sequence: cagcagctgcctgagaagtacaaagag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          4397..4427
                     /label="ABE-s4"
                     /note="sequence: ggaaaacgaggacattctggaagatatcgtg"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(4900..4922)
                     /label="HNH-R1"
                     /note="sequence: ttcgatccgcttcattctctcgc"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          5198..5222
                     /label="ABE-s5"
                     /note="sequence: gaagatgaagaactactggcggcag"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          5812..5834
                     /label="D125-F1"
                     /note="sequence: ccgtgcggaaagtgctgagcatg"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          5817..5834
                     /label="D328-F1"
                     /note="sequence: cggaaagtgctgagcatg"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          5999..6021
                     /label="ABE-s6"
                     /note="sequence: ggtggtggccaaagtggaaaagg"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(6667..6691)
                     /label="D328-R1"
                     /note="sequence: cttttaaatgagccgccagAGTCAC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          6673..6691
                     /label="D328-F2-2amp"
                     /note="sequence: ctggcggctcatttaaaag"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          6673..6709
                     /label="D328-F2"
                     /note="sequence: ctggcggctcatttaaaagATATTTAgggTCCAGcAC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    6684..6743
                     /label="MTS"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    6684..7952
                     /label="ARG8_nuclear"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(7933..7961)
                     /label="D328-R2"
                     /note="sequence: GGGCTTGCCAGCGTAAACcgcttcaatag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          7944..7970
                     /label="D328-F3"
                     /note="sequence: GTTTACGCTGGCAAGCCCatccccaac"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    7953..7994
                     /label="V5 tag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(8051..8068)
                     /label="D328-R3"
                     /note="sequence: GccttgCTCACCATAGGC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             8055..8771
                     /label="EGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             8055..8774
                     /label="Translation 8055-8774"
                     /translation="MVSKAEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*"
     misc_feature    complement(8101..8121)
                     /label="EGFP-N"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(8101..8121)
                     /label="EGFP-N primer"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    8708..8729
                     /label="EGFP-C-For primer"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    8708..8729
                     /label="EGFP-C-For"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     complement(9046..9065)
                     /label="T7"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     complement(9046..9066)
                     /label="T7"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     primer_bind     complement(9075..9092)
                     /label="M13-fwd"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     CDS             9163..9231
                     /label="LacZ alpha"
                     /ApEinfo_revcolor="#7ea7d8"
                     /ApEinfo_fwdcolor="#7ea7d8"
     rep_origin      9232..9687
                     /label="M13 origin"
                     /ApEinfo_revcolor="#8493ca"
                     /ApEinfo_fwdcolor="#8493ca"
     rep_origin      9249..9555
                     /label="F1 ori"
                     /ApEinfo_revcolor="#8493ca"
                     /ApEinfo_fwdcolor="#8493ca"
     gene            9988..11591
                     /label="LEU2"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     rep_origin      complement(12225..12735)
                     /label="CEN-ARS pRS"
                     /ApEinfo_revcolor="#f49ac2"
                     /ApEinfo_fwdcolor="#f49ac2"
     CDS             13065..13724
                     /label="AmpR"
                     /ApEinfo_revcolor="#fff79a"
                     /ApEinfo_fwdcolor="#fff79a"
     rep_origin      13822..607
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#8493ca"
                     /ApEinfo_fwdcolor="#8493ca"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtTct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttaGc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcGtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctcag tttatcatta tcaatactcg ccatttcaaa
     1081 gaatacgtaa ataattaata gtagtgattt tcctaacttt atttagtcaa aaaattagcc
     1141 ttttaattct gctgtaaccc gtacatgccc aaaatagggg gcgggttaca cagaatatat
     1201 aacaTcgTag gtgTctgggt gaacagttta ttcctggcat ccactaaata taatggagcc
     1261 cgctttttaa gctggcatcc agaaaaaaaa agaatcccag caccaaaata ttgttttctt
     1321 caccaaccat cagttcatag gtccattctc ttagcgcaac tacagagaac aggggcacaa
     1381 acaggcaaaa aacgggcaca acctcaatgg agtgatgcaa cctgcctgga gtaaatgatg
     1441 acacaaggca attgacccac gcatgtatct atctcatttt cttacacctt ctattacctt
     1501 ctgctctctc tgatttggaa aaagctgaaa aaaaaggttg aaaccagttc cctgaaatta
     1561 ttcccctact tgactaataa gtatataaag acggtaggta ttgattgtaa ttctgtaaat
     1621 ctatttctta aacttcttaa attctacttt tatagttagt ctttttttta gttttaaaac
     1681 accagaactt agtttcgacg gattctagaa ctagtggatc ccccatcaca agtttgtaca
     1741 aaaaagcAGG CTTCGAAGGA GATAACAAAA TGGCCTCCAC TCGTGTCCTC GCCTCTCGCC
     1801 TGGCCTCCCA GATGGCTGCT TCCGCCAAGG TTGCCCGCCC TGCTGTCCGC GTTGCTCAGG
     1861 TCAGCAAGCG CACCATCCAG ACTGGCTCCC CCCTCCAGAC CCTCAAGCGC ACCCAGATGA
     1921 CCTCCATCGT CAACGCCACC ACCCGCCAGG CTTTCCAGAA GCGCGCCTAC TCTTCCtctg
     1981 aggtggagtt ttcccacgag tactggatga gacatgccct gaccctggcc aagagggcac
     2041 gggatgagag ggaggtgcct gtgggagccg tgctggtgct gaacaataga gtgatcggcg
     2101 agggctggaa cagagccatc ggcctgcacg acccaacagc ccatgccgaa attatggccc
     2161 tgagacaggg cggcctggtc atgcagaact acagactgat tgacgccacc ctgtacgtga
     2221 cattcgagcc ttgcgtgatg tgcgccggcg ccatgatcca ctctaggatc ggccgcgtgg
     2281 tgtttggcTG Gaggaactca aaaagaggcg ccgcaggctc cctgatgaac gtgctgaact
     2341 accccggcat gaatcaccgc gtcgaaatta ccgagggaat cctggcagat gaatgtgccg
     2401 ccctgctgtg cgatttctat cggatgccta gacaggtgtt caatgctcag aagaaggccc
     2461 agagctccat caactccgga ggatctagcg gaggctcctc tggctctgag acacctggca
     2521 caagcgagag cgcaacacct gaaagcagcg ggggcagcag cggggggtca gacaagaagt
     2581 acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt
     2641 acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga
     2701 agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc acccggctga
     2761 agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga
     2821 tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct
     2881 tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg
     2941 aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca
     3001 gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc
     3061 ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt
     3121 tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc aacgccagcg
     3181 gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gAGCAGACGG CTGGAAAATC
     3241 TGATCGCCCA GCTgcccggc gagaagaaga atggcctgtt cggaaacctg attgccctga
     3301 gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat gccaaactgc
     3361 agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag atcggcgacc
     3421 agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg ctgagcgaca
     3481 tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg atcaagagat
     3541 acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg
     3601 agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc tacattgacg
     3661 gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa aagatggacg
     3721 gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag cagcggacct
     3781 tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc attctgcggc
     3841 ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag aagatcctga
     3901 ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga ttcgcctgga
     3961 tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg gtggacaagg
     4021 gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac ctgcccaacg
     4081 agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat aacgagctga
     4141 ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc ggcgagcaga
     4201 aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga
     4261 aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag
     4321 atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc aaggacaagg
     4381 acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg accctgacac
     4441 tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg
     4501 acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg ctgagccgga
     4561 agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat ttcctgaagt
     4621 ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc ctgaccttta
     4681 aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac gagcacattg
     4741 ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg aaggtggtgg
     4801 acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc gaaatggcca
     4861 gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg aagcggatcg
     4921 aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc
     4981 agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat atgtacgtgg
     5041 accaggaact ggacatcaac cggctgtccg actacgatgt ggaccatatc gtgcctcaga
     5101 gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac aagaaccggg
     5161 gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac tactggcggc
     5221 agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc aaggccgaga
     5281 gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg gtggaaaccc
     5341 ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg
     5401 agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg
     5461 atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc
     5521 acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg
     5581 aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga
     5641 gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact
     5701 ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct ctgatcgaga
     5761 caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc accgtgcgga
     5821 aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct
     5881 tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc agaaagaagg
     5941 actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat tctgtgctgg
     6001 tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg
     6061 ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt ctggaagcca
     6121 agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac tccctgttcg
     6181 agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag aagggaaacg
     6241 aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac tatgagaagc
     6301 tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag cacaagcact
     6361 acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc ctggccgacg
     6421 ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc atcagagagc
     6481 aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct gccgccttca
     6541 agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag gtgctggacg
     6601 ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac ctgtctcagc
     6661 tgggagGTGA CTctggcggc tcatttaaaa gATATTTAgg gTCCAGcACG TCATCAAGAA
     6721 GATTTACAAG CATTTTAGAG GAAAAGGCCT TTCAAGTGAC CACTTACTCT AGACCTGAAG
     6781 ATCTATGTAT AACTAGAGGT AAAAATGCAA AGCTGTATGA TGACGTGAAT GGTAAAGAAT
     6841 ATATCGATTT CACCGCAGGT ATTGCGGTGA CCGCATTAGG CCATGCAAAT CCTAAAGTGG
     6901 CAGAAATTCT GCACCATCAG GCTAACAAAC TGGTTCATTC CTCCAACCTT TACTTCACTA
     6961 AGGAATGTTT GGATTTAAGT GAAAAGATTG TTGAAAAGAC CAAGCAATTC GGTGGTCAAC
     7021 ACGACGCCTC AAGAGTATTT TTATGTAATT CTGGTACGGA AGCAAATGAA GCTGCTTTGA
     7081 AGTTTGCAAA GAAACATGGT ATAATGAAAA ATCCTAGCAA GCAAGGCATT GTTGCATTTG
     7141 AGAACTCTTT TCATGGCCGT ACTATGGGCG CTTTATCTGT CACTTGGAAT AGTAAATATA
     7201 GAACTCCTTT TGGGGATTTG GTTCCCCATG TCTCATTCTT AAATTTGAAT GACGAAATGA
     7261 CCAAACTACA AAGTTATATC GAGACCAAAA AGGACGAGAT TGCTGGTTTA ATTGTCGAGC
     7321 CCATACAAGG TGAAGGTGGG GTTTTTCCCG TAGAAGTTGA AAAGCTAACC GGATTGAAGA
     7381 AAATATGTCA AGATAATGAT GTGATTGTCA TTCATGATGA AATTCAATGC GGTTTGGGCC
     7441 GTTCAGGTAA ACTATGGGCT CATGCTTATT TACCAAGTGA GGCTCATCCG GATATTTTTA
     7501 CATCTGCCAA AGCATTGGGA AATGGCTTCC CCATCGCTGC CACCATCGTC AATGAAAAAG
     7561 TTAATAATGC TTTGAGAGTT GGTGACCACG GCACCACGTA TGGTGGTAAT CCGCTGGCCT
     7621 GTTCTGTAAG CAACTATGTT TTGGATACCA TAGCAGACGA AGCTTTTTTG AAACAAGTCT
     7681 CTAAGAAGAG TGATATCTTA CAAAAGCGCT TGCGCGAAAT TCAAGCCAAA TATCCAAATC
     7741 AAATAAAGAC TATCAGAGGA AAAGGTTTGA TGCTTGGTGC TGAGTTCGTC GAACCACCCA
     7801 CCGAGGTCAT CAAAAAGGCC AGAGAATTGG GACTTTTGAT CATTACCGCT GGTAAGAGTA
     7861 CCGTTAGATT TGTTCCCGCA TTAACGATTG AAGACGAACT AATCGAAGAA GGGATGGATG
     7921 CTTTTGAAAA GGctattgaa gcgGTTTACG CTGGCAAGCC Catccccaac CCCCTGCTGG
     7981 GCCTGGACAG CACCGGTGCA ACAAACTTCT CCCTCTTGAA ACAAGCAGGA GATGTTGAGG
     8041 AAAATCCCGG GCCTATGGTG AGcaaggCcg aggagctgtt caccggggtg gtgcccatcc
     8101 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     8161 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     8221 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     8281 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     8341 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     8401 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     8461 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     8521 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     8581 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     8641 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     8701 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     8761 agctgtacaa gtaactcgag tcatgtaatt agttatgtca cgcttacatt cacgccctcc
     8821 ccccacatcc gctctaaccg aaaaggaagg agttagacaa cctgaagtct aggtccctat
     8881 ttattttttt atagttatgt tagtattaag aacgttattt atatttcaaa tttttctttt
     8941 ttttctgtac agacgcgtgt acgcatgtaa cattatactg aaaaccttgc ttgagaaggt
     9001 tttgggacgc tcgaaggctt taatttgcgg ccggtaccca attcgcccta tagtgagtcg
     9061 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     9121 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     9181 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gacgcgccct
     9241 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     9301 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     9361 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     9421 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     9481 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     9541 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     9601 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     9661 ttaacaaaat attaacgCtt acaatttcct gatgcggtat tttctcctta cgcatctgtg
     9721 cggtatttca caccgcatat cgacCggtcg aggagaactt ctagtatatc Tacataccta
     9781 atattattgc cttattaaaa atggaatccc aacaattaca tcaaaatcca cattctcttc
     9841 aaaatcaatt gtcctgtact tccttgttca tgtgtgttca aaaacgttat atttatagga
     9901 taattatact ctatttctca acaagtaatt ggttgtttgg ccgagcggtc taaggcgcct
     9961 gattcaagaa atatcttgac cgcagttaac tgtgggaata ctcaggtatc gtaagatgca
    10021 agagttcgaa tctcttagca accattattt ttttcctcaa cataacgaga acacacaggg
    10081 gcgctatcgc acagaatcaa attcgatgac tggaaatttt ttgttaattt cagaggtcgc
    10141 ctgacgcata tacctttttc aactgaaaaa ttgggagaaa aaggaaaggt gagagCgccg
    10201 gaaccggctt ttcatataga atagagaagc gttcatgact aaatgcttgc atcacaatac
    10261 ttgaagttga caatattatt taaggaccta ttgttttttc caataggtgg ttagcaatcg
    10321 tcttactttc taacttttct taccttttac atttcagcaa tatatatata tatAtTTcaa
    10381 ggatatacca ttctAatgtc tgcccctaag aagatcgtcg ttttgccagg tgaccacgtt
    10441 ggtcaagaaa tcacagccga agccattaag gttcttaaag ctatttctga tgttcgttcc
    10501 aatgtcaagt tcgatttcga aaatcattta attggtggtg ctgctatcga tgctacaggt
    10561 gtTccacttc cagatgaggc gctggaagcc tccaagaagg Ctgatgccgt tttgttaggt
    10621 gctgtgggtg gtcctaaatg gggtaccggt agtgttagac ctgaacaagg tttactaaaa
    10681 atccgtaaag aacttcaatt gtacgccaac ttaagaccat gtaactttgc atccgactct
    10741 cttttagact tatctccaat caagccacaa tttgctaaag gtactgactt cgttgttgtc
    10801 agagaattag tgggaggtat ttactttggt aagagaaagg aagacgatgg tgatggtgtc
    10861 gcttgggata gtgaacaata caccgttcca gaagtgcaaa gaatcacaag aatggccgct
    10921 ttcatggccc tacaacatga gccaccattg cctatttggt ccttggataa agctaatgtt
    10981 ttggcctctt caagattatg gagaaaaact gtggaggaaa ccatcaagaa cgaattccct
    11041 acattgaagg ttcaacatca attgattgat tctgccgcca tgatcctagt taagaaccca
    11101 acccacctaa atggtattat aatcaccagc aacatgtttg gtgatatcat ctccgatgaa
    11161 gcctccgtta tcccaggttc cttgggtttg ttgccatctg cgtccttggc ctctttgcca
    11221 gacaagaaca ccgcatttgg tttgtacgaa ccatgccacg gttctgctcc agatttgcca
    11281 aagaataagg tCAaccctat cgccactatc ttgtctgctg caatgatgtt gaaattgtca
    11341 ttgaacttgc ctgaagaagg taaggccatt gaagatgcag ttaaaaaggt tttggatgca
    11401 ggtatcagaa ctggtgattt aggtggttcc aacagtacca ccgaagtcgg tgatgctgtc
    11461 gccgaagaag ttaagaaaat ccttgcttaa aaagattctc tttttttatg atatttgtac
    11521 ataaacttta taaatgaaat tcataataga aacgacacga aattacaaaa tggaatatgt
    11581 tcatagggta gacgaaacta tatacgcaat ctacatacat ttatcaagaa ggagaaaaag
    11641 gaggatgtaa aggaatacag gtaagcaaat tgatactaat ggctcaacgt gataaggaaa
    11701 aagaattgca ctttaacatt aatattgaca aggaggaggg caccacacaa aaagttaggt
    11761 gtaacagaaa atcatgaaac taTgattcct aatttATATA tTgGAgGAtt tTCTCTaaaa
    11821 aaaaaaaata caacaaataa aaaacactca atgacctgac catttgatgg agtttaagtc
    11881 aataccttct tgaaccattt cccataatgg tgaaagttcc ctcaagaatt ttactctgtc
    11941 agaaacggcc ttAacgacgt agtcgatatg gtgcactctc agtacaatct gctctgatgc
    12001 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
    12061 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
    12121 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
    12181 tttataggtt aatgtcatga taataatggt ttcttaggac ggatcgcttg cctgtaactt
    12241 acacgcgcct cgtatctttt aatgatggaa taatttggga atttactctg tgtttattta
    12301 tttttatgtt ttgtatttgg attttagaaa gtaaataaag aaggtagaag agttacggaa
    12361 tgaagaaaaa aaaataaaca aaggtttaaa aaatttcaac aaaaagcgta ctttacatat
    12421 atatttatta gacaagaaaa gcagattaaa tagatataca ttcgattaac gataagtaaa
    12481 atgtaaaatc acaggatttt cgtgtgtggt cttctacaca gacaagatga aacaattcgg
    12541 cattaatacc tgagagcagg aagagcaaga taaaaggtag tatttgttgg cgatccccct
    12601 agagtctttt acatcttcgg aaaacaaaaa ctattttttc tttaatttct ttttttactt
    12661 tctattttta atttatatat ttatattaaa aaatttaaat tataattatt tttatagcac
    12721 gtgatgaaaa ggacccaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
    12781 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
    12841 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
    12901 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
    12961 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
    13021 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
    13081 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
    13141 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
    13201 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
    13261 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
    13321 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
    13381 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
    13441 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
    13501 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
    13561 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
    13621 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
    13681 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
    13741 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
    13801 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
    13861 gagcgtcaga ccccgtagaa aagatcaaag gatcttc
//