LOCUS       PSY124-pAG415GPD-Arg8nu 8939 bp ds-DNA     circular     05-AUG-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      complement(73..583)
                     /label="CEN-ARS pRS"
                     /ApEinfo_revcolor="#f49ac2"
                     /ApEinfo_fwdcolor="#f49ac2"
     CDS             913..1572
                     /label="AmpR"
                     /ApEinfo_revcolor="#fff79a"
                     /ApEinfo_fwdcolor="#fff79a"
     rep_origin      1670..2352
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#8493ca"
                     /ApEinfo_fwdcolor="#8493ca"
     misc_binding    2692..2714
                     /label="LacO"
                     /ApEinfo_revcolor="#7ea7d8"
                     /ApEinfo_fwdcolor="#7ea7d8"
     primer_bind     2720..2740
                     /label="M13-rev"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     promoter        2757..2775
                     /label="T3 promoter"
                     /ApEinfo_revcolor="#8882be"
                     /ApEinfo_fwdcolor="#8882be"
     primer_bind     2758..2777
                     /label="T3"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     misc_feature    2794..3443
                     /label="GPD_promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          3318..3367
                     /label="SY32-seq-F1-GPDF"
                     /note="sequence: GACTAATAAGTATATAAAGACGGTAGGTATTGATTGTAATTCTGTAAATC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          3470..3500
                     /label="GUF"
                     /note="sequence: GTACAAAAAAGCAGGCTTCGAAGGAGATAAC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3474..3498
                     /label="attB1_invitrogen"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_recomb     3475..3493
                     /label="attR1"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_recomb     3475..3493
                     /label="attB1"
                     /ApEinfo_revcolor="#bc8dbf"
                     /ApEinfo_fwdcolor="#bc8dbf"
     misc_feature    3478..3497
                     /label="ATTB1 primer"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_recomb     3479..3493
                     /label="attB1_short"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    3504..4773
                     /label="Arg8 nuclear"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             3504..4773
                     /label="Translation 3504-4773"
                     /translation="MFKRYLSSTSSRRFTSILEEKAFQVTTYSRPEDLCITRGKNAKLYDDVNGKEYIDFTAGIAVTALGHANPKVAEILHHQANKLVHSSNLYFTKECLDLSEKIVEKTKQFGGQHDASRVFLCNSGTEANEAALKFAKKHGIMKNPSKQGIVAFENSFHGRTMGALSVTWNSKYRTPFGDLVPHVSFLNLNDEMTKLQSYIETKKDEIAGLIVEPIQGEGGVFPVEVEKLTGLKKICQDNDVIVIHDEIQCGLGRSGKLWAHAYLPSEAHPDIFTSAKALGNGFPIAATIVNEKVNNALRVGDHGTTYGGNPLACSVSNYVLDTIADEAFLKQVSKKSDILQKRLREIQAKYPNQIKTIRGKGLMLGAEFVEPPTEVIKKARELGLLIITAGKSTVRFVPALTIEDELIEEGMDAFEKAIEAVYA"
     CDS             4773..4787
                     /label="Translation 4773-4787"
                     /translation="NPAFL"
     misc_feature    complement(4774..4797)
                     /label="ATTB2 primer"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    4774..4798
                     /label="attB2_invitrogen"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_recomb     complement(4777..4797)
                     /label="attB2"
                     /ApEinfo_revcolor="#bc8dbf"
                     /ApEinfo_fwdcolor="#bc8dbf"
     misc_feature    complement(4777..4797)
                     /label="attR2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             4788..4829
                     /label="Translation 4788-4829"
                     /translation="YKVVMGCRNSISSL"
     CDS             4830..5546
                     /label="EGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             4830..5549
                     /label="Translation 4830-5549"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*"
     primer          4843..4862
                     /label="SY28-seq-F3"
                     /note="sequence: gcgaggagctgttcaccggg"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(4876..4896)
                     /label="EGFP-N"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(4876..4896)
                     /label="EGFP-N primer"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    5483..5504
                     /label="EGFP-C-For primer"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    5483..5504
                     /label="EGFP-C-For"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     complement(5821..5840)
                     /label="T7"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     complement(5821..5841)
                     /label="T7"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     primer_bind     complement(5850..5867)
                     /label="M13-fwd"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     CDS             5938..6006
                     /label="LacZ alpha"
                     /ApEinfo_revcolor="#7ea7d8"
                     /ApEinfo_fwdcolor="#7ea7d8"
     rep_origin      6011..6466
                     /label="M13 origin"
                     /ApEinfo_revcolor="#8493ca"
                     /ApEinfo_fwdcolor="#8493ca"
     rep_origin      6028..6334
                     /label="F1 ori"
                     /ApEinfo_revcolor="#8493ca"
                     /ApEinfo_fwdcolor="#8493ca"
     gene            6766..8378
                     /label="LEU2"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata
      121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt
      181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa
      241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata
      301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct
      361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata
      421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact
      481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa
      541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg
      601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1861 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     2521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     2581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttacctcact
     2641 cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg tggaattgtg
     2701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     2761 aaccctcact aaagggaaca aaagctggag ctcagtttat cattatcaat actcgccatt
     2821 tcaaagaata cgtaaataat taatagtagt gattttccta actttattta gtcaaaaaat
     2881 tagcctttta attctgctgt aacccgtaca tgcccaaaat agggggcggg ttacacagaa
     2941 tatataacac cgaaggtgcc tgggtgaaca ggtttattcc tggcatccac taaatataat
     3001 ggagcccgct ttttaagctg gcatccagaa aaaaaaagaa tcccagcacc aaaatattgt
     3061 tttcttcacc aaccatcagt tcataggtcc attctcttag cgcaactaca gagaacaggg
     3121 gcacaaacag gcaaaaaacg ggcacaacct caatggagtg atgcaacctg cctggagtaa
     3181 atgatgacac aaggcaattg acccacgcat gtatctatct cattttctta caccttctat
     3241 taccttctgc tctctctgat ttggaaaaag ctgaaaaaaa aggttgaaac cagttccctg
     3301 aaattattcc cctacttgac taataagtat ataaagacgg taggtattga ttgtaattct
     3361 gtaaatctat ttcttaaact tcttaaattc tacttttata gttagtcttt tttttagttt
     3421 taaaacacca gaacttagtt tcgacggatt ctagaactag tggatccccc atcacaagtt
     3481 tgtacaaaaa agcAGGCTAC AAAATGTTTA AAAGATATTT ATCCAGTACG TCATCAAGAA
     3541 GATTTACAAG CATTTTAGAG GAAAAGGCCT TTCAAGTGAC CACTTACTCT AGACCTGAAG
     3601 ATCTATGTAT AACTAGAGGT AAAAATGCAA AGCTGTATGA TGACGTGAAT GGTAAAGAAT
     3661 ATATCGATTT CACCGCAGGT ATTGCGGTGA CCGCATTAGG CCATGCAAAT CCTAAAGTGG
     3721 CAGAAATTCT GCACCATCAG GCTAACAAAC TGGTTCATTC CTCCAACCTT TACTTCACTA
     3781 AGGAATGTTT GGATTTAAGT GAAAAGATTG TTGAAAAGAC CAAGCAATTC GGTGGTCAAC
     3841 ACGACGCCTC AAGAGTATTT TTATGTAATT CTGGTACGGA AGCAAATGAA GCTGCTTTGA
     3901 AGTTTGCAAA GAAACATGGT ATAATGAAAA ATCCTAGCAA GCAAGGCATT GTTGCATTTG
     3961 AGAACTCTTT TCATGGCCGT ACTATGGGCG CTTTATCTGT CACTTGGAAT AGTAAATATA
     4021 GAACTCCTTT TGGGGATTTG GTTCCCCATG TCTCATTCTT AAATTTGAAT GACGAAATGA
     4081 CCAAACTACA AAGTTATATC GAGACCAAAA AGGACGAGAT TGCTGGTTTA ATTGTCGAGC
     4141 CCATACAAGG TGAAGGTGGG GTTTTTCCCG TAGAAGTTGA AAAGCTAACC GGATTGAAGA
     4201 AAATATGTCA AGATAATGAT GTGATTGTCA TTCATGATGA AATTCAATGC GGTTTGGGCC
     4261 GTTCAGGTAA ACTATGGGCT CATGCTTATT TACCAAGTGA GGCTCATCCG GATATTTTTA
     4321 CATCTGCCAA AGCATTGGGA AATGGCTTCC CCATCGCTGC CACCATCGTC AATGAAAAAG
     4381 TTAATAATGC TTTGAGAGTT GGTGACCACG GCACCACGTA TGGTGGTAAT CCGCTGGCCT
     4441 GTTCTGTAAG CAACTATGTT TTGGATACCA TAGCAGACGA AGCTTTTTTG AAACAAGTCT
     4501 CTAAGAAGAG TGATATCTTA CAAAAGCGCT TGCGCGAAAT TCAAGCCAAA TATCCAAATC
     4561 AAATAAAGAC TATCAGAGGA AAAGGTTTGA TGCTTGGTGC TGAGTTCGTC GAACCACCCA
     4621 CCGAGGTCAT CAAAAAGGCC AGAGAATTGG GACTTTTGAT CATTACCGCT GGTAAGAGTA
     4681 CCGTTAGATT TGTTCCCGCA TTAACGATTG AAGACGAACT AATCGAAGAA GGGATGGATG
     4741 CTTTTGAAAA GGCTATTGAA GCGGTTTACG CTAACCcagc tttcttgtac aaagtggtga
     4801 tgggctgcag gaattcgata tcaagcttaa tggtgagcaa gggcgaggag ctgttcaccg
     4861 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     4921 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     4981 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     5041 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     5101 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     5161 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     5221 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     5281 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     5341 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     5401 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     5461 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     5521 ctctcggcat ggacgagctg tacaagtaac tcgagtcatg taattagtta tgtcacgctt
     5581 acattcacgc cctcccccca catccgctct aaccgaaaag gaaggagtta gacaacctga
     5641 agtctaggtc cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt
     5701 tcaaattttt cttttttttc tgtacagacg cgtgtacgca tgtaacatta tactgaaaac
     5761 cttgcttgag aaggttttgg gacgctcgaa ggctttaatt tgcggccggt acccaattcg
     5821 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     5881 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     5941 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     6001 gaatggcgcg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     6061 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     6121 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     6181 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     6241 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     6301 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     6361 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     6421 caaaaattta acgcgaattt taacaaaata ttaacgttta caatttcctg atgcggtatt
     6481 ttctccttac gcatctgtgc ggtatttcac accgcatatc gacggtcgag gagaacttct
     6541 agtatatcca catacctaat attattgcct tattaaaaat ggaatcccaa caattacatc
     6601 aaaatccaca ttctcttcaa aatcaattgt cctgtacttc cttgttcatg tgtgttcaaa
     6661 aacgttatat ttataggata attatactct atttctcaac aagtaattgg ttgtttggcc
     6721 gagcggtcta aggcgcctga ttcaagaaat atcttgaccg cagttaactg tgggaatact
     6781 caggtatcgt aagatgcaag agttcgaatc tcttagcaac cattattttt ttcctcaaca
     6841 taacgagaac acacaggggc gctatcgcac agaatcaaat tcgatgactg gaaatttttt
     6901 gttaatttca gaggtcgcct gacgcatata cctttttcaa ctgaaaaatt gggagaaaaa
     6961 ggaaaggtga gaggccggaa ccggcttttc atatagaata gagaagcgtt catgactaaa
     7021 tgcttgcatc acaatacttg aagttgacaa tattatttaa ggacctattg ttttttccaa
     7081 taggtggtta gcaatcgtct tactttctaa cttttcttac cttttacatt tcagcaatat
     7141 atatatatat ttcaaggata taccattcta atgtctgccc ctatgtctgc ccctaagaag
     7201 atcgtcgttt tgccaggtga ccacgttggt caagaaatca cagccgaagc cattaaggtt
     7261 cttaaagcta tttctgatgt tcgttccaat gtcaagttcg atttcgaaaa tcatttaatt
     7321 ggtggtgctg ctatcgatgc tacaggtgtc ccacttccag atgaggcgct ggaagcctcc
     7381 aagaaggttg atgccgtttt gttaggtgct gtgggtggtc ctaaatgggg taccggtagt
     7441 gttagacctg aacaaggttt actaaaaatc cgtaaagaac ttcaattgta cgccaactta
     7501 agaccatgta actttgcatc cgactctctt ttagacttat ctccaatcaa gccacaattt
     7561 gctaaaggta ctgacttcgt tgttgtcaga gaattagtgg gaggtattta ctttggtaag
     7621 agaaaggaag acgatggtga tggtgtcgct tgggatagtg aacaatacac cgttccagaa
     7681 gtgcaaagaa tcacaagaat ggccgctttc atggccctac aacatgagcc accattgcct
     7741 atttggtcct tggataaagc taatgttttg gcctcttcaa gattatggag aaaaactgtg
     7801 gaggaaacca tcaagaacga attccctaca ttgaaggttc aacatcaatt gattgattct
     7861 gccgccatga tcctagttaa gaacccaacc cacctaaatg gtattataat caccagcaac
     7921 atgtttggtg atatcatctc cgatgaagcc tccgttatcc caggttcctt gggtttgttg
     7981 ccatctgcgt ccttggcctc tttgccagac aagaacaccg catttggttt gtacgaacca
     8041 tgccacggtt ctgctccaga tttgccaaag aataaggttg accctatcgc cactatcttg
     8101 tctgctgcaa tgatgttgaa attgtcattg aacttgcctg aagaaggtaa ggccattgaa
     8161 gatgcagtta aaaaggtttt ggatgcaggt atcagaactg gtgatttagg tggttccaac
     8221 agtaccaccg aagtcggtga tgctgtcgcc gaagaagtta agaaaatcct tgcttaaaaa
     8281 gattctcttt ttttatgata tttgtacata aactttataa atgaaattca taatagaaac
     8341 gacacgaaat tacaaaatgg aatatgttca tagggtagac gaaactatat acgcaatcta
     8401 catacattta tcaagaagga gaaaaaggag gatagtaaag gaatacaggt aagcaaattg
     8461 atactaatgg ctcaacgtga taaggaaaaa gaattgcact ttaacattaa tattgacaag
     8521 gaggagggca ccacacaaaa agttaggtgt aacagaaaat catgaaacta cgattcctaa
     8581 tttgatattg gaggattttc tctaaaaaaa aaaaaataca acaaataaaa aacactcaat
     8641 gacctgacca tttgatggag tttaagtcaa taccttcttg aaccatttcc cataatggtg
     8701 aaagttccct caagaatttt actctgtcag aaacggcctt acgacgtagt cgatatggtg
     8761 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     8821 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     8881 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcga
//