LOCUS       LNE244-BiFC6            5844 bp    DNA     circular SYN 08-JAN-2026
DEFINITION  LNE244-BiFC6.
ACCESSION   LNE244-BiFC6
VERSION     LNE244-BiFC6
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     CDS             3488..3952
                     /note="pLannotate"
                     /label="VN155(I152L)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /description="improved N-terminal fragment of mVenus for
                     use in bimolecular fluorescence complementation (BiFC)
                     (Kodama and Hu, 2010)"
     CDS             complement(1758..2615)
                     /note="pLannotate"
                     /label="BLAT_ECOLX"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /description="Protein evidence at protein level: Swiss-Prot
                     protein existence level 1. TEM-type are the most prevalent
                     beta-lactamases in enterobacteria; they hydrolyze the
                     beta-lactam bond in susceptible beta-lactam antibiotics,
                     thus conferring resistance to penicillins and
                     cephalosporins. TEM-3 and TEM-4 are capable of hydrolyzing
                     cefotaxime and ceftazidime. TEM-5 is capable of hydrolyzing
                     ceftazidime. TEM-6 is capable of hydrolyzing ceftazidime
                     and aztreonam. TEM-8/CAZ-2, TEM-16/CAZ-7 and TEM-24/CAZ-6
                     are markedly active against ceftazidime. IRT-4 shows
                     resistance to beta-lactamase inhibitors. From Escherichia
                     coli."
     CDS             4247..4498
                     /note="pLannotate"
                     /label="VC155"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /description="C-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
     rep_origin      1..772
                     /note="pLannotate"
                     /label="pBBR1 oriV"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.3"
                     /fragment="False"
                     /other="rep_origin"
                     /description="replication origin of the broad-host-range
                     plasmid pBBR1 from Bordetella bronchiseptica; requires the
                     pBBR1 Rep protein for replication"
     CDS             773..1435
                     /note="pLannotate"
                     /label="pBBR1 Rep"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /description="replication protein for the broad-host-range
                     plasmid pBBR1 from Bordetella bronchiseptica"
     terminator      complement(2780..2874)
                     /note="pLannotate"
                     /label="lambda t0 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
                     /description="transcription terminator from phage lambda"
     promoter        complement(2616..2707)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /description="bla"
     terminator      5644..5730
                     /note="pLannotate"
                     /label="rrnB T1 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
                     /description="Escherichia coli rrnB; transcription
                     terminator T1 from the E. coli rrnB gene"
     terminator      complement(1712..1751)
                     /note="pLannotate"
                     /label="fd terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
                     /description="central terminator from bacteriophage fd
                     (Otsuka and Kunisawa, 1982)"
     oriT            complement(1446..1554)
                     /note="pLannotate"
                     /label="oriT"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="99.1"
                     /fragment="False"
                     /other="oriT"
                     /description="incP origin of transfer"
     CDS             3257..3478
                     /note="pLannotate"
                     /label="B2_FHV (fragment)"
                     /database="swissprot"
                     /identity="98.6"
                     /match_length="69.8"
                     /fragment="True"
                     /other="CDS"
                     /description="Protein evidence at protein level: Swiss-Prot
                     protein existence level 1. Binds double-strand RNA (dsRNA)
                     with high affinity. Suppresses the host RNA silencing-based
                     antiviral response. From Flock house virus (FHV)."
     CDS             4016..4237
                     /note="pLannotate"
                     /label="B2_FHV (fragment)"
                     /database="swissprot"
                     /identity="98.6"
                     /match_length="69.8"
                     /fragment="True"
                     /other="CDS"
                     /description="Protein evidence at protein level: Swiss-Prot
                     protein existence level 1. Binds double-strand RNA (dsRNA)
                     with high affinity. Suppresses the host RNA silencing-based
                     antiviral response. From Flock house virus (FHV)."
     promoter        4579..4652
                     /note="pLannotate"
                     /label="lacIq promoter (fragment)"
                     /database="snapgene"
                     /identity="98.6"
                     /match_length="94.9"
                     /fragment="True"
                     /other="promoter"
                     /description="lacI (mutant); In the lacIq allele, a single
                     base change in the promoter boosts expression of the lacI
                     gene about 10-fold."
     misc_RNA        3157..3206
                     /note="pLannotate"
                     /label="sTRSV HHRz"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="96.2"
                     /fragment="False"
                     /other="misc_RNA"
                     /description="hammerhead ribozyme from the tobacco ringspot
                     virus satellite RNA (Khvorova et al., 2003)"
     promoter        3116..3140
                     /note="pLannotate"
                     /label="J23119(SpeI) promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="71.4"
                     /fragment="True"
                     /other="promoter"
                     /description="bacterial promoter (Registry of Standard
                     Biological Parts BBa J23119) modified to end with an SpeI
                     site"
ORIGIN
        1 gcggccaccg gctggctcgc ttcgctcggc ccgtggacaa ccctgctgga caagctgatg
       61 gacaggctgc gcctgcccac gagcttgacc acagggattg cccaccggct acccagcctt
      121 cgaccacata cccaccggct ccaactgcgc ggcctgcggc cttgccccat caattttttt
      181 aattttctct ggggaaaagc ctccggcctg cggcctgcgc gcttcgcttg ccggttggac
      241 accaagtgga aggcgggtca aggctcgcgc agcgaccgcg cagcggcttg gccttgacgc
      301 gcctggaacg acccaagcct atgcgagtgg gggcagtcga agggcgaagc ccgcccgcct
      361 gccccccgag cctcacggcg gcgagtgcgg gggttccaag ggggcagcgc caccttgggc
      421 aaggccgaag gccgcgcagt cgatcaacaa gccccggagg ggccactttt tgccggaggg
      481 ggagccgcgc cgaaggcgtg ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga
      541 actagatata gggcgaaatg cgaaagactt aaaaatcaac aacttaaaaa aggggggtac
      601 gcaacagctc attgcggcac cccccgcaat agctcattgc gtaggttaaa gaaaatctgt
      661 aattgactgc cacttttacg caacgcataa ttgttgtcgc gctgccgaaa agttgcagct
      721 gattgcgcat ggtgccgcaa ccgtgcggca cccctaccgc atggagataa gcatggccac
      781 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
      841 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
      901 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
      961 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
     1021 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
     1081 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
     1141 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
     1201 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
     1261 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
     1321 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
     1381 ggacgatggc gagccgttgg agccgccgac acgggtaacg ctgccgcgcc ggtagggccg
     1441 gcctacggcc agcctcgcag agcaggattc ccgttgagca ccgccaggtg cgaataaggg
     1501 acagtgaaga aggaacaccc gctcgcgggt gggcctactt cacctatcct gcccggctga
     1561 cgccgttgga tacaccaagg aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt
     1621 gcgaaaaagg atggatatac cgaaaaaatc gctataatga ccccgaagca gggttatgca
     1681 gcggaaaagg acaacgcgcg gaccggggtc gtccaaaaaa aaaggctcca aaaggagcct
     1741 ttaattgtat cggtttacca atgtttaatc aggctcgcgc caatttccgc aatctgacgg
     1801 ttacgttcat ccatcgtcgc ctggctgccg gtggtataaa tcaccacaat acggctcggt
     1861 ttaccatccg ggcccagcgc cgcaataatg ccacggctgc cacgttcgcc cgcaccgctt
     1921 ttatccgcaa taaaccagcc agccggcagc gcgctacgca gcagcggacc cgccacttta
     1981 tccgcttcca tccaatcaat cagttgctgg cggcttgcca gggtcagcag ctcacccgtc
     2041 agcagtttac gcagggtggt cgccattgct gccggcatgg tggtatcacg ttcatcgttc
     2101 ggaatcgctt cgttcagttc cggttcccaa cgatccagac gggtcacatg atcgcccatg
     2161 ttatgcagaa acgcggtcag ttctttcgga ccgccaatgg tcgtcagcag caggttcgcc
     2221 gcggtgttat cgctcatggt aatcgccgcg ctgcacagtt cacgcacggt catgccatcg
     2281 gtcagatgtt tttcggtcac cgggctatat tccaccagat cgttctggct ataatgaata
     2341 cgacggccca gttgttcctg gcccgcatcc acacggctca gaaccgcacc gcacagcagc
     2401 actttaaagg tgctcatcat cggaaaacgt tcttccggac gaaaagattc cagaattttg
     2461 ccgctgttca gatccagttc aatatagccc acgcgcgcac ccagttgatc ttccgcatct
     2521 ttcactttca ccagggtttc cggatgcgca aacaccggca ggcaaaacgc cgcaaaaaac
     2581 ggaatcagcg ccacacgaaa atgctgaatg ctcatactct tcctttttca atattattga
     2641 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     2701 aaacaaatag gggaattaaa aaaaagcccg ctcattaggc gggctactaa tttaaatcgt
     2761 aattattggg gacccctgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc
     2821 tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaaggt
     2881 tctcttgcag gtggcacctg cttcgcatct agactgatga gacgtggtag agccacaaac
     2941 agccggtaca agcaacgatc tccaggacca tctgaatcat gcgcggatga cacgaactca
     3001 cgacggcgat cacagacatt aacccacagt acagacactg cgacaacgtg gcaattcgtc
     3061 gcaatacaac ggtttctggt atcccgctta acgatcgttg gctgattgga tccaattgac
     3121 agctagctca gtcctaggta ccattggatc caatagctgt caccggatgt gctttccggt
     3181 ctgatgagtc cgtgaggacg aaacagcctc tacaaataat tttgtttaat actagagaaa
     3241 gaggggaaat actagtatgc cctccaaact tgcccttatc caagaacttc ccgatcggat
     3301 tcaaaccgct gttgaagcag ctatgggcat gtcgtatcaa gccgcgccca acaatgtgcg
     3361 tcgggacctt gacaacctgc acgcctgcct taacaaggcc aaacttactg tctcgcgtat
     3421 ggtcacgagc ctgcttgaaa agcctagcgt ggtagcatac ctggagggca aagcgcccgg
     3481 cagatctatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     3541 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     3601 cacctacggc aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg
     3661 gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc ttcgcccgct accccgacca
     3721 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     3781 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     3841 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3901 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatctcaccg cctgattgta
     3961 ttgcgacgaa ttgccattct atacagaata ctagagaaag aggggaaata ctagtatgcc
     4021 aagcaaactc gcgctaatcc aggaacttcc cgaccgcatt caaacggcgg tggaagcagc
     4081 catgggaatg agctaccaag ccgcaccgaa caacgtgcgc agggacctcg acaacctgca
     4141 cgcttgccta aacaaggcaa aactaacggt aagtcggatg gtaacatcac tgctggagaa
     4201 acccagcgtg gtggcatacc tagagggaaa ggccccccga ggatccgaca agcagaagaa
     4261 cggcatcaag gccaacttca agatccgcca caacatcgag gacggcggcg tgcagctcgc
     4321 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     4381 ctacctgagc taccagtcca aactgagcaa agaccccaac gagaagcgcg atcacatggt
     4441 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     4501 aatccctcgg taccaaattc cagaaaagag gcctcccgaa aggggggcct tttttcgttt
     4561 tggtccaatg gcggcgcgcc atcgaatggt gcaaaacctt tcgcggtatg gcatgatagc
     4621 gcccggaaga gagtcaattc aggggggtga ataatggaca tgcctcgtat taaaccgggt
     4681 cagcgtgtta tgatggcact gcgtaaaatg attgcaagcg gtgaaatcaa aagtggtgaa
     4741 cgtattgcag aaattccgac cgcagcagca ctgggtgtta gccgtatgcc ggttcgtatc
     4801 gcactgcgtt cactggaaca agaaggtctg gttgttcgtc tgggtgcacg tggttatgca
     4861 gcccgtggtg ttagcagcga tcagattcgt gatgcaattg aagttcgtgg tgttctggaa
     4921 ggttttgcag cacgtcgtct ggcagaacgt ggtatgaccg cagaaaccca tgcacgtttt
     4981 gttgtactga ttgcagaagg tgaagcactg tttgcagccg gtcgcctgaa tggtgaagat
     5041 ctggatcgtt atgccgcata taatcaggca tttcatgata ccctggttag cgcagcaggt
     5101 aatggtgcag ttgaaagcgc actggcacgt aatggttttg aaccgtttgc agcagccggt
     5161 gcactggccc tggatctgat ggacctgtct gccgaatatg aacatctgct ggcagcacat
     5221 cgtcagcatc aggcagttct ggatgcagtt agctgtggtg atgccgaagg tgcagaacgt
     5281 attatgcgtg atcatgcact ggcagcaatt cgtaatgcaa aagtttttga agcagcagca
     5341 agcgcaggcg caccgctggg tgcagcatgg tcaattcgtg cagattgata aggatcctaa
     5401 ttggtaacga atcagacaat tgacggctcg agggagtagg ctggaaatct gctcgtcagt
     5461 ggtgctcaca ctgacgaatc atgtacagat cataccgatg actgcctggc gactcacaac
     5521 taagcaagac agccggaacc agcgccggcg aacaccactg catatatggc atatcacaac
     5581 agtccacgtc tcaagcagtt acagagatgt tacgaacgcg catcacctgc aaggcgaagg
     5641 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     5701 tcggtgaacg ctctcctgag taggacaaat ccgccgccct agacagctgg gcgcgccccc
     5761 cctacgggct tgctctccgg gcttcgccct gcgcggtcgc tgcgctccct tgccagcccg
     5821 tggatatgtg gacgatggcc gcga
//
