LOCUS       LNE379-BiFC7b           5983 bp    DNA     circular SYN 08-JAN-2026
DEFINITION  LNE379-BiFC7b.
ACCESSION   LNE379-BiFC7b
VERSION     LNE379-BiFC7b
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     CDS             complement(3072..3950)
                     /note="pLannotate"
                     /label="araC"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /description="L-arabinose regulatory protein; araC"
     CDS             4561..5025
                     /note="pLannotate"
                     /label="VN155(I152L)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /description="improved N-terminal fragment of mVenus for
                     use in bimolecular fluorescence complementation (BiFC)
                     (Kodama and Hu, 2010)"
     CDS             complement(1758..2615)
                     /note="pLannotate"
                     /label="BLAT_ECOLX"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /description="Protein evidence at protein level: Swiss-Prot
                     protein existence level 1. TEM-type are the most prevalent
                     beta-lactamases in enterobacteria; they hydrolyze the
                     beta-lactam bond in susceptible beta-lactam antibiotics,
                     thus conferring resistance to penicillins and
                     cephalosporins. TEM-3 and TEM-4 are capable of hydrolyzing
                     cefotaxime and ceftazidime. TEM-5 is capable of hydrolyzing
                     ceftazidime. TEM-6 is capable of hydrolyzing ceftazidime
                     and aztreonam. TEM-8/CAZ-2, TEM-16/CAZ-7 and TEM-24/CAZ-6
                     are markedly active against ceftazidime. IRT-4 shows
                     resistance to beta-lactamase inhibitors. From Escherichia
                     coli."
     CDS             5320..5571
                     /note="pLannotate"
                     /label="VC155"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /description="C-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
     rep_origin      1..772
                     /note="pLannotate"
                     /label="pBBR1 oriV"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.3"
                     /fragment="False"
                     /other="rep_origin"
                     /description="replication origin of the broad-host-range
                     plasmid pBBR1 from Bordetella bronchiseptica; requires the
                     pBBR1 Rep protein for replication"
     CDS             773..1435
                     /note="pLannotate"
                     /label="pBBR1 Rep"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /description="replication protein for the broad-host-range
                     plasmid pBBR1 from Bordetella bronchiseptica"
     terminator      complement(2780..2874)
                     /note="pLannotate"
                     /label="lambda t0 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
                     /description="transcription terminator from phage lambda"
     promoter        complement(2616..2707)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /description="bla"
     terminator      5783..5869
                     /note="pLannotate"
                     /label="rrnB T1 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
                     /description="Escherichia coli rrnB; transcription
                     terminator T1 from the E. coli rrnB gene"
     promoter        3977..4261
                     /note="pLannotate"
                     /label="araBAD promoter"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /description="araBAD; promoter of the L-arabinose operon of
                     E. coli; the araC regulatory gene is transcribed in the
                     opposite direction (Guzman et al., 1995)"
     terminator      complement(1712..1751)
                     /note="pLannotate"
                     /label="fd terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
                     /description="central terminator from bacteriophage fd
                     (Otsuka and Kunisawa, 1982)"
     oriT            complement(1446..1554)
                     /note="pLannotate"
                     /label="oriT"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="99.1"
                     /fragment="False"
                     /other="oriT"
                     /description="incP origin of transfer"
     RBS             4288..4310
                     /note="pLannotate"
                     /label="RBS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="RBS"
                     /description="efficient ribosome binding site from
                     bacteriophage T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4330..4551
                     /note="pLannotate"
                     /label="B2_FHV (fragment)"
                     /database="swissprot"
                     /identity="98.6"
                     /match_length="69.8"
                     /fragment="True"
                     /other="CDS"
                     /description="Protein evidence at protein level: Swiss-Prot
                     protein existence level 1. Binds double-strand RNA (dsRNA)
                     with high affinity. Suppresses the host RNA silencing-based
                     antiviral response. From Flock house virus (FHV)."
     CDS             5089..5310
                     /note="pLannotate"
                     /label="B2_FHV (fragment)"
                     /database="swissprot"
                     /identity="98.6"
                     /match_length="69.8"
                     /fragment="True"
                     /other="CDS"
                     /description="Protein evidence at protein level: Swiss-Prot
                     protein existence level 1. Binds double-strand RNA (dsRNA)
                     with high affinity. Suppresses the host RNA silencing-based
                     antiviral response. From Flock house virus (FHV)."
ORIGIN
        1 gcggccaccg gctggctcgc ttcgctcggc ccgtggacaa ccctgctgga caagctgatg
       61 gacaggctgc gcctgcccac gagcttgacc acagggattg cccaccggct acccagcctt
      121 cgaccacata cccaccggct ccaactgcgc ggcctgcggc cttgccccat caattttttt
      181 aattttctct ggggaaaagc ctccggcctg cggcctgcgc gcttcgcttg ccggttggac
      241 accaagtgga aggcgggtca aggctcgcgc agcgaccgcg cagcggcttg gccttgacgc
      301 gcctggaacg acccaagcct atgcgagtgg gggcagtcga agggcgaagc ccgcccgcct
      361 gccccccgag cctcacggcg gcgagtgcgg gggttccaag ggggcagcgc caccttgggc
      421 aaggccgaag gccgcgcagt cgatcaacaa gccccggagg ggccactttt tgccggaggg
      481 ggagccgcgc cgaaggcgtg ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga
      541 actagatata gggcgaaatg cgaaagactt aaaaatcaac aacttaaaaa aggggggtac
      601 gcaacagctc attgcggcac cccccgcaat agctcattgc gtaggttaaa gaaaatctgt
      661 aattgactgc cacttttacg caacgcataa ttgttgtcgc gctgccgaaa agttgcagct
      721 gattgcgcat ggtgccgcaa ccgtgcggca cccctaccgc atggagataa gcatggccac
      781 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
      841 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
      901 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
      961 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
     1021 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
     1081 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
     1141 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
     1201 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
     1261 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
     1321 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
     1381 ggacgatggc gagccgttgg agccgccgac acgggtaacg ctgccgcgcc ggtagggccg
     1441 gcctacggcc agcctcgcag agcaggattc ccgttgagca ccgccaggtg cgaataaggg
     1501 acagtgaaga aggaacaccc gctcgcgggt gggcctactt cacctatcct gcccggctga
     1561 cgccgttgga tacaccaagg aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt
     1621 gcgaaaaagg atggatatac cgaaaaaatc gctataatga ccccgaagca gggttatgca
     1681 gcggaaaagg acaacgcgcg gaccggggtc gtccaaaaaa aaaggctcca aaaggagcct
     1741 ttaattgtat cggtttacca atgtttaatc aggctcgcgc caatttccgc aatctgacgg
     1801 ttacgttcat ccatcgtcgc ctggctgccg gtggtataaa tcaccacaat acggctcggt
     1861 ttaccatccg ggcccagcgc cgcaataatg ccacggctgc cacgttcgcc cgcaccgctt
     1921 ttatccgcaa taaaccagcc agccggcagc gcgctacgca gcagcggacc cgccacttta
     1981 tccgcttcca tccaatcaat cagttgctgg cggcttgcca gggtcagcag ctcacccgtc
     2041 agcagtttac gcagggtggt cgccattgct gccggcatgg tggtatcacg ttcatcgttc
     2101 ggaatcgctt cgttcagttc cggttcccaa cgatccagac gggtcacatg atcgcccatg
     2161 ttatgcagaa acgcggtcag ttctttcgga ccgccaatgg tcgtcagcag caggttcgcc
     2221 gcggtgttat cgctcatggt aatcgccgcg ctgcacagtt cacgcacggt catgccatcg
     2281 gtcagatgtt tttcggtcac cgggctatat tccaccagat cgttctggct ataatgaata
     2341 cgacggccca gttgttcctg gcccgcatcc acacggctca gaaccgcacc gcacagcagc
     2401 actttaaagg tgctcatcat cggaaaacgt tcttccggac gaaaagattc cagaattttg
     2461 ccgctgttca gatccagttc aatatagccc acgcgcgcac ccagttgatc ttccgcatct
     2521 ttcactttca ccagggtttc cggatgcgca aacaccggca ggcaaaacgc cgcaaaaaac
     2581 ggaatcagcg ccacacgaaa atgctgaatg ctcatactct tcctttttca atattattga
     2641 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     2701 aaacaaatag gggaattaaa aaaaagcccg ctcattaggc gggctactaa tttaaatcgt
     2761 aattattggg gacccctgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc
     2821 tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaaggt
     2881 tctcttgcag gtggcacctg cttcgcatct agactgatga gacgtggtag agccacaaac
     2941 agccggtaca agcaacgatc tccaggacca tctgaatcat gcgcggatga cacgaactca
     3001 cgacggcgat cacagacatt aacccacagt acagacactg cgacaacgtg gcaattcgtc
     3061 gcaatacaac gttatgacaa cttgacggct acatcattca ctttttcttc acaaccggca
     3121 cggaactcgc tcgggctggc cccggtgcat tttttaaata cccgcgagaa atagagttga
     3181 tcgtcaaaac caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc
     3241 agcttcgcct ggctgatacg ttggtcctcg cgccagctta agacgctaat ccctaactgc
     3301 tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa catgctgtgc gacgctggcg
     3361 atatcaaaat tgctgtctgc caggtgatcg ctgatgtact gacaagcctc gcgtacccga
     3421 ttatccatcg gtggatggag cgactcgtta atcgcttcca tgcgccgcag taacaattgc
     3481 tcaagcagat ttatcgccag cagctccgaa tagcgccctt ccccttgccc ggcgttaatg
     3541 atttgcccaa acaggtcgct gaaatgcggc tggtgcgctt catccgggcg aaagaacccc
     3601 gtattggcaa atattgacgg ccagttaagc cattcatgcc agtaggcgcg cggacgaaag
     3661 taaacccact ggtgatacca ttcgcgagcc tccggatgac gaccgtagtg atgaatctct
     3721 cctggcggga acagcaaaat atcacccggt cggcaaacaa attctcgtcc ctgatttttc
     3781 accaccccct gaccgcgaat ggtgagattg agaatataac ctttcattcc cagcggtcgg
     3841 tcgataaaaa aatcgagata accgttggcc tcaatcggcg ttaaacccgc caccagatgg
     3901 gcattaaacg agtatcccgg cagcagggga tcattttgcg cttcagccat acttttcata
     3961 ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc
     4021 gtcttttact ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa aagcattctg
     4081 taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc
     4141 agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta
     4201 tccataagat tagcggatac tacctgacgc tttttatcgc aactctctac tgtttctcca
     4261 tacccgtttt tttgggctag aaataatttt gtttaacttt aagaaggaga tatacatatg
     4321 cggggttcta tgccctccaa acttgccctt atccaagaac ttcccgatcg gattcaaacc
     4381 gctgttgaag cagctatggg catgtcgtat caagccgcgc ccaacaatgt gcgtcgggac
     4441 cttgacaacc tgcacgcctg ccttaacaag gccaaactta ctgtctcgcg tatggtcacg
     4501 agcctgcttg aaaagcctag cgtggtagca tacctggagg gcaaagcgcc cggcagatct
     4561 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     4621 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     4681 ggcaagctga ccctgaagct gatctgcacc accggcaagc tgcccgtgcc ctggcccacc
     4741 ctcgtgacca ccctgggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag
     4801 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     4861 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     4921 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     4981 aagctggagt acaactacaa cagccacaac gtctatctca ccgcctgatt gtattgcgac
     5041 gaattgccat tctatacaga atactagaga aagaggggaa atactagtat gccaagcaaa
     5101 ctcgcgctaa tccaggaact tcccgaccgc attcaaacgg cggtggaagc agccatggga
     5161 atgagctacc aagccgcacc gaacaacgtg cgcagggacc tcgacaacct gcacgcttgc
     5221 ctaaacaagg caaaactaac ggtaagtcgg atggtaacat cactgctgga gaaacccagc
     5281 gtggtggcat acctagaggg aaaggccccc cgaggatccg acaagcagaa gaacggcatc
     5341 aaggccaact tcaagatccg ccacaacatc gaggacggcg gcgtgcagct cgccgaccac
     5401 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     5461 agctaccagt ccaaactgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     5521 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaatccgc
     5581 tggaaatctg ctcgtcagtg gtgctcacac tgacgaatca tgtacagatc ataccgatga
     5641 ctgcctggcg actcacaact aagcaagaca gccggaacca gcgccggcga acaccactgc
     5701 atatatggca tatcacaaca gtccacgtct caagcagtta cagagatgtt acgaacgcgc
     5761 atcacctgca aggcgaaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     5821 tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgcccta
     5881 gacagctggg cgcgcccccc ctacgggctt gctctccggg cttcgccctg cgcggtcgct
     5941 gcgctccctt gccagcccgt ggatatgtgg acgatggccg cga
//
