LOCUS       Exported               16204 bp ds-DNA     circular SYN 09-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jan 14, 2026 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16204
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    405..411
                     /label=misc_feature 1
     LTR             828..1016
                     /label=5' LTR
                     /note="5' LTR (R and U5 portions; U3 was replaced by the 
                     CMV promoter)"
     misc_feature    1067..1204
                     /label=Psi
                     /note="Psi"
     misc_feature    1124..1127
                     /label=SD; splice donor
                     /note="SD; splice donor"
     misc_feature    2440..2557
                     /label=CPPT; central polypurine tract
                     /note="CPPT; central polypurine tract"
     promoter        2607..2862
                     /label=EFS-NS
                     /note="EFS-NS"
     CDS             2886..6989
                     /codon_start=1
                     /label=hSpCas9
                     /note="hSpCas9"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6990..7037
                     /codon_start=1
                     /label=NLS
                     /note="NLS"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7038..7061
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG"
                     /translation="DYKDDDDK"
     CDS             7062..7172
                     /codon_start=1
                     /label=GS_XTEN_GS_linker
                     /translation="SGGSSGSGSETPGTSESATPESSGGSSSGGGSGGSGS"
     CDS             7173..10406
                     /codon_start=1
                     /label=ZRANB3
                     /translation="PRVHNIKKSLTPHISCVTNESDNLLDFLPDRLRAKLLPFQKDGII
                     FALKRNGRCMVADEMGLGKTIQAIGITYFYKEEWPLLIVVPSSLRYPWTEEIEKWIPEL
                     SPEEINVIQNKTDVRRMSTSKVTVLGYGLLTADAKTLIDALNNQNFKVVIVDESHYMKS
                     RNATRSRILLPIVQKARRAILLTGTPALGRPEELFMQIEALFPQKFGRWTDYAKRYCNA
                     HIRYFGKRPQWDCRGASNLNELHQLLSDIMIRRLKTEVLTQLPPKVRQRIPFDLPSAAA
                     KELNTSFEEWEKIMRTPNSGAMETVMGLITRMFKQTAIAKAGAVKDYIKMMLQNDSLKF
                     LVFAHHLSMLQACTEAVIENKTRYIRIDGSVSSSERIHLVNQFQKDPDTRVAILSIQAA
                     GQGLTFTAASHVVFAELYWDPGHIKQAEDRAHRIGQCSSVNIHYLIANGTLDTLMWGML
                     NRKAQVTGSTLNGRKEKIQAEEGDKEKWDFLQFAEAWTPNDSSEELRKEALFTHFEKEK
                     QHDIRSFFVPQPKKRQLMTSCDESKRFREENTVVSSDPTKTAARDIIDYESDVEPETKR
                     LKLAASEDHCSPSEETPSQSKQIRTPLVESVQEAKAQLTTPAFPVEGWQCSLCTYINNS
                     ELPYCEMCETPQGSAVMQIDSLNHIQDKNEKDDSQKDTSKKVQTISDCEKQALAQSEPG
                     QLADSKEETPKIEKEDGLTSQPGNEQWKSSDTLPVYDTLMFCASRNTDRIHIYTKDGKQ
                     MSCNFIPLDIKLDLWEDLPASFQLKQYRSLILRFVREWSSLTAMKQRIIRKSGQLFCSP
                     ILALEEITKQQTKQNCTKRYITKEDVAVASMDKVKNVGGHVRLITKESRPRDPFTKKLL
                     EDGACVPFLNPYTVQADLTVKPSTSKGYLQAVDNEGNPLCLRCQQPTCQTKQACKANSW
                     DSRFCSLKCQEEFWIRSNNSYLRAKVFETEHGVCQLCNVNAQELFLRLRDAPKSQRKNL
                     LYATWTSKLPLEQLNEMIRNPGEGHFWQVDHIKPVYGGGGQCSLDNLQTLCTVCHKERT
                     ARQAKERSQVRRQSLASKHGSDITRFLVKK"
     CDS             10407..10472
                     /codon_start=1
                     /label=P2A
                     /note="P2A"
                     /translation="GSGATNFSLLKQAGDVEENPGP"
     CDS             10473..10871
                     /codon_start=1
                     /label=Bla
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    10894..11487
                     /label=WPRE; Woodchuck post-transcriptional
                     /note="WPRE; Woodchuck post-transcriptional regulatory 
                     element"
     LTR             12002..12190
                     /label=3' LTR
                     /note="3' LTR"
     rep_origin      14639..14864
                     /direction=RIGHT
                     /label=ColE1
                     /note="ColE1"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagctagg tcttgaaagg agtgggaatt ggctccggtg
     2641 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
     2701 gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
     2761 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
     2821 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggaccggttc tagagcgctg
     2881 ccaccatgga caagaagtac agcatcggcc tggacatcgg caccaactct gtgggctggg
     2941 ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg
     3001 accggcacag catcaagaag aacctgatcg gagccctgct gttcgacagc ggcgaaacag
     3061 ccgaggccac ccggctgaag agaaccgcca gaagaagata caccagacgg aagaaccgga
     3121 tctgctatct gcaagagatc ttcagcaacg agatggccaa ggtggacgac agcttcttcc
     3181 acagactgga agagtccttc ctggtggaag aggataagaa gcacgagcgg caccccatct
     3241 tcggcaacat cgtggacgag gtggcctacc acgagaagta ccccaccatc taccacctga
     3301 gaaagaaact ggtggacagc accgacaagg ccgacctgcg gctgatctat ctggccctgg
     3361 cccacatgat caagttccgg ggccacttcc tgatcgaggg cgacctgaac cccgacaaca
     3421 gcgacgtgga caagctgttc atccagctgg tgcagaccta caaccagctg ttcgaggaaa
     3481 accccatcaa cgccagcggc gtggacgcca aggccatcct gtctgccaga ctgagcaaga
     3541 gcagacggct ggaaaatctg atcgcccagc tgcccggcga gaagaagaat ggcctgttcg
     3601 gaaacctgat tgccctgagc ctgggcctga cccccaactt caagagcaac ttcgacctgg
     3661 ccgaggatgc caaactgcag ctgagcaagg acacctacga cgacgacctg gacaacctgc
     3721 tggcccagat cggcgaccag tacgccgacc tgtttctggc cgccaagaac ctgtccgacg
     3781 ccatcctgct gagcgacatc ctgagagtga acaccgagat caccaaggcc cccctgagcg
     3841 cctctatgat caagagatac gacgagcacc accaggacct gaccctgctg aaagctctcg
     3901 tgcggcagca gctgcctgag aagtacaaag agattttctt cgaccagagc aagaacggct
     3961 acgccggcta cattgacggc ggagccagcc aggaagagtt ctacaagttc atcaagccca
     4021 tcctggaaaa gatggacggc accgaggaac tgctcgtgaa gctgaacaga gaggacctgc
     4081 tgcggaagca gcggaccttc gacaacggca gcatccccca ccagatccac ctgggagagc
     4141 tgcacgccat tctgcggcgg caggaagatt tttacccatt cctgaaggac aaccgggaaa
     4201 agatcgagaa gatcctgacc ttccgcatcc cctactacgt gggccctctg gccaggggaa
     4261 acagcagatt cgcctggatg accagaaaga gcgaggaaac catcaccccc tggaacttcg
     4321 aggaagtggt ggacaagggc gcttccgccc agagcttcat cgagcggatg accaacttcg
     4381 ataagaacct gcccaacgag aaggtgctgc ccaagcacag cctgctgtac gagtacttca
     4441 ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga gggaatgaga aagcccgcct
     4501 tcctgagcgg cgagcagaaa aaggccatcg tggacctgct gttcaagacc aaccggaaag
     4561 tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat cgagtgcttc gactccgtgg
     4621 aaatctccgg cgtggaagat cggttcaacg cctccctggg cacataccac gatctgctga
     4681 aaattatcaa ggacaaggac ttcctggaca atgaggaaaa cgaggacatt ctggaagata
     4741 tcgtgctgac cctgacactg tttgaggaca gagagatgat cgaggaacgg ctgaaaacct
     4801 atgcccacct gttcgacgac aaagtgatga agcagctgaa gcggcggaga tacaccggct
     4861 ggggcaggct gagccggaag ctgatcaacg gcatccggga caagcagtcc ggcaagacaa
     4921 tcctggattt cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag ctgatccacg
     4981 acgacagcct gacctttaaa gaggacatcc agaaagccca ggtgtccggc cagggcgata
     5041 gcctgcacga gcacattgcc aatctggccg gcagccccgc cattaagaag ggcatcctgc
     5101 agacagtgaa ggtggtggac gagctcgtga aagtgatggg ccggcacaag cccgagaaca
     5161 tcgtgatcga aatggccaga gagaaccaga ccacccagaa gggacagaag aacagccgcg
     5221 agagaatgaa gcggatcgaa gagggcatca aagagctggg cagccagatc ctgaaagaac
     5281 accccgtgga aaacacccag ctgcagaacg agaagctgta cctgtactac ctgcagaatg
     5341 ggcgggatat gtacgtggac caggaactgg acatcaaccg gctgtccgac tacgatgtgg
     5401 accatatcgt gcctcagagc tttctgaagg acgactccat cgacaacaag gtgctgacca
     5461 gaagcgacaa gaaccggggc aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga
     5521 tgaagaacta ctggcggcag ctgctgaacg ccaagctgat tacccagaga aagttcgaca
     5581 atctgaccaa ggccgagaga ggcggcctga gcgaactgga taaggccggc ttcatcaaga
     5641 gacagctggt ggaaacccgg cagatcacaa agcacgtggc acagatcctg gactcccgga
     5701 tgaacactaa gtacgacgag aatgacaagc tgatccggga agtgaaagtg atcaccctga
     5761 agtccaagct ggtgtccgat ttccggaagg atttccagtt ttacaaagtg cgcgagatca
     5821 acaactacca ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc gccctgatca
     5881 aaaagtaccc taagctggaa agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc
     5941 ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc taccgccaag tacttcttct
     6001 acagcaacat catgaacttt ttcaagaccg agattaccct ggccaacggc gagatccgga
     6061 agcggcctct gatcgagaca aacggcgaaa ccggggagat cgtgtgggat aagggccggg
     6121 attttgccac cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg aaaaagaccg
     6181 aggtgcagac aggcggcttc agcaaagagt ctatcctgcc caagaggaac agcgataagc
     6241 tgatcgccag aaagaaggac tgggacccta agaagtacgg cggcttcgac agccccaccg
     6301 tggcctattc tgtgctggtg gtggccaaag tggaaaaggg caagtccaag aaactgaaga
     6361 gtgtgaaaga gctactgggg atcaccatca tggaaagaag cagcttcgag aagaatccca
     6421 tcgactttct ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc
     6481 ctaagtactc cctgttcgag ctggaaaacg gccggaagag aatgctggcc tctgccggcg
     6541 aactgcagaa gggaaacgaa ctggccctgc cctccaaata tgtgaacttc ctgtacctgg
     6601 ccagccacta tgagaagctg aagggctccc ccgaggataa tgagcagaaa cagctgtttg
     6661 tggaacagca caagcactac ctggacgaga tcatcgagca gatcagcgag ttctccaaga
     6721 gagtgatcct ggccgacgct aatctggaca aagtgctgtc cgcctacaac aagcaccggg
     6781 ataagcccat cagagagcag gccgagaata tcatccacct gtttaccctg accaatctgg
     6841 gagcccctgc cgccttcaag tactttgaca ccaccatcga ccggaagagg tacaccagca
     6901 ccaaagaggt gctggacgcc accctgatcc accagagcat caccggcctg tacgagacac
     6961 ggatcgacct gtctcagctg ggaggcgaca agcgacctgc cgccacaaag aaggctggac
     7021 aggctaagaa gaagaaagat tacaaagacg atgacgataa gtctggagga tctagcggat
     7081 ctggaagcga gacaccagga acaagcgagt cagcaacacc agagagcagt ggcggcagca
     7141 gctcgggagg tggttcgggt ggatctggat cacctagggt tcataacata aaaaagtctc
     7201 ttacacctca catttcttgt gtgacaaatg aatctgataa tctgctggat tttttgcctg
     7261 acagactaag agcaaagcta cttccattcc agaaagatgg catcattttt gccctcaaaa
     7321 gaaatggcag gtgtatggtg gctgatgaaa tgggtctagg aaagacaatc caggcaattg
     7381 gaattactta cttctataaa gaggaatggc ctctgttaat agtggtccct tcgtctctga
     7441 ggtacccttg gacagaagaa attgaaaaat ggatcccaga gctaagtcca gaagaaatca
     7501 atgttattca gaataaaact gatgttagga gaatgtcgac cagtaaagtg acagttctgg
     7561 gttatggtct cttaaccgca gatgcaaaga ctttgataga tgcactgaat aatcagaact
     7621 tcaaagtagt tatagtggat gaatcacact acatgaaatc cagaaatgca actcgcagca
     7681 ggattttatt gccaatagta cagaaagcca gacgagccat tcttcttaca ggaacaccag
     7741 ctttaggaag gcctgaagag ctttttatgc agattgaagc tctctttcca caaaaatttg
     7801 gaagatggac cgactatgca aaaagatact gtaatgcaca catcagatat tttggtaaaa
     7861 gacctcagtg ggattgtaga ggggcatcaa atcttaatga acttcaccag ctattaagtg
     7921 acataatgat tagaagatta aagactgaag ttttaaccca gctaccccct aaagtcagac
     7981 agcgtattcc atttgatctt ccatcagcag ctgccaagga attgaatacc agctttgaag
     8041 agtgggaaaa aataatgaga actccaaatt caggtgccat ggagacagtc atggggttga
     8101 taactcgcat gtttaaacaa actgctattg ccaaggcagg tgctgtaaag gattatatta
     8161 agatgatgct tcagaatgat tcgcttaaat ttctggtttt tgctcaccat ttaagcatgc
     8221 tccaagcttg cacagaagca gtcatcgaaa ataagactcg ttacattagg atagatggaa
     8281 gtgtttcatc ttcagaaaga atacatctgg ttaatcagtt tcaaaaggat cctgacactc
     8341 gcgtggctat cctaagcatt caggctgctg gccagggatt aacatttact gcagcaagtc
     8401 atgttgtatt tgctgagttg tactgggacc ctggacatat aaaacaagca gaagaccgag
     8461 ctcacagaat tggccagtgc agttctgtga atattcacta ccttattgca aatggaaccc
     8521 tagacaccct tatgtgggga atgttgaatc gcaaggctca agttacaggg agcacactga
     8581 acggtaggaa agaaaaaatt caggctgagg aaggtgataa ggaaaaatgg gatttcctgc
     8641 agtttgctga agcttggact ccaaatgaca gttctgaaga gttaaggaag gaagctttgt
     8701 tcactcactt cgaaaaagaa aaacagcatg atattcgatc attttttgta ccacaaccta
     8761 aaaaaagaca gttgatgacc tcctgtgacg aatcaaagag attccgggag gaaaatactg
     8821 tagtgtcatc agaccctaca aaaacagctg caagagatat catcgattat gaaagtgatg
     8881 ttgaacctga aacgaaaaga ttgaaattgg ctgcctcgga agaccactgc agtccgtcgg
     8941 aagagacacc atcccagtcc aagcaaatcc gaactccact cgtggaaagt gtacaggagg
     9001 ccaaggccca gttaaccacc ccagcctttc ctgtagaggg ctggcaatgt agtctctgca
     9061 cctatatcaa taattcagag ttaccttatt gtgaaatgtg tgagactcct caaggcagtg
     9121 ctgttatgca aatagatagc ctcaaccata tccaggataa aaacgagaag gatgattctc
     9181 agaaagacac ctccaaaaag gttcaaacta tctcagactg tgaaaaacaa gcccttgcac
     9241 agtcagaacc tggccagttg gctgacagca aggaagaaac accaaaaatt gagaaagaag
     9301 acggacttac atcccagcca ggtaatgaac agtggaagag ttcagacact ttgccagtgt
     9361 atgacacctt aatgttctgt gcaagtagga atactgaccg gattcacatc tatactaagg
     9421 atggaaaaca gatgagctgt aatttcattc ctctggatat aaaattagac ctttgggaag
     9481 atttaccagc aagctttcag ctgaaacaat atcgctcact gattttgaga tttgttcgag
     9541 aatggagtag tctaactgcc atgaagcaaa ggataatcag gaaaagtgga cagctattct
     9601 gtagcccaat tcttgctttg gaagagatca caaagcaaca aaccaaacaa aattgcacca
     9661 aaagatacat aaccaaagaa gatgttgccg tagcctcaat ggacaaagtg aagaatgttg
     9721 ggggccatgt ccgtctgatc acaaaggagt ccaggccacg ggatcctttc acaaaaaaac
     9781 ttcttgaaga tggagcctgt gtcccatttc taaatccata cacagtccag gcagatctca
     9841 ctgtgaagcc ctctacatcc aaaggctatt tgcaagctgt ggataatgaa ggaaatccac
     9901 tttgccttcg ctgtcagcaa cccacttgcc agactaagca agcatgtaaa gcgaactctt
     9961 gggattcacg gttttgctct ctgaaatgtc aggaagagtt ttggattcga tctaataaca
    10021 gttacctgag agccaaagta tttgaaactg aacatggtgt gtgtcagctc tgtaatgtga
    10081 acgcacaaga actcttttta cgtctgagag atgcccctaa aagtcagagg aagaatcttc
    10141 tgtatgctac ctggacttca aagctcccat tagaacagct aaatgaaatg ataagaaacc
    10201 caggggaagg acatttctgg caggtggatc acatcaagcc agtgtatggg ggaggaggac
    10261 agtgttccct ggacaacctg cagactctct gcacagtctg tcacaaagag agaactgcca
    10321 gacaagctaa ggaaagaagc caggtgagaa gacaatctct agcatcaaag catggatcag
    10381 acatcacacg atttttggta aagaagggat ccggcgcaac aaacttctct ctgctgaaac
    10441 aagccggaga tgtcgaagag aatcctggac cgatggccaa gcctttgtct caagaagaat
    10501 ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca
    10561 gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc
    10621 attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag
    10681 ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct
    10741 gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg
    10801 acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt
    10861 gggagggcta agaattcgat atcaagctta tcggtaatca acctctggat tacaaaattt
    10921 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
    10981 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
    11041 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
    11101 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
    11161 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
    11221 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
    11281 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
    11341 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
    11401 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
    11461 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg
    11521 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac
    11581 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt
    11641 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
    11701 ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
    11761 tccctgattg gcagaactac acaccagggc cagggatcag atatccactg acctttggat
    11821 ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga
    11881 acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat
    11941 tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg
    12001 actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
    12061 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
    12121 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
    12181 tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
    12241 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
    12301 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
    12361 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
    12421 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
    12481 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
    12541 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
    12601 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
    12661 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
    12721 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
    12781 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
    12841 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
    12901 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
    12961 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
    13021 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
    13081 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
    13141 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
    13201 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
    13261 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta
    13321 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc
    13381 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt
    13441 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt
    13501 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac
    13561 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt
    13621 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg
    13681 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga
    13741 gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg
    13801 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
    13861 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
    13921 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    13981 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
    14041 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
    14101 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
    14161 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
    14221 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
    14281 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
    14341 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
    14401 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
    14461 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
    14521 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    14581 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    14641 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    14701 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    14761 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    14821 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    14881 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
    14941 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    15001 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    15061 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
    15121 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
    15181 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
    15241 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
    15301 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
    15361 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
    15421 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
    15481 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
    15541 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    15601 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    15661 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    15721 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    15781 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    15841 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    15901 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    15961 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    16021 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    16081 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    16141 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    16201 tgac
//
