LOCUS       Exported               13793 bp ds-DNA     circular SYN 08-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jan 14, 2026 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13793
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    405..411
                     /label=misc_feature 1
     LTR             828..1016
                     /label=5' LTR
                     /note="5' LTR (R and U5 portions; U3 was replaced by the 
                     CMV promoter)"
     misc_feature    1067..1204
                     /label=Psi
                     /note="Psi"
     misc_feature    1124..1127
                     /label=SD; splice donor
                     /note="SD; splice donor"
     misc_feature    2440..2557
                     /label=CPPT; central polypurine tract
                     /note="CPPT; central polypurine tract"
     promoter        2608..2863
                     /label=EFS-NS
                     /note="EFS-NS"
     CDS             2887..6990
                     /codon_start=1
                     /label=hSpCas9
                     /note="hSpCas9"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6991..7038
                     /codon_start=1
                     /label=NLS
                     /note="NLS"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7039..7062
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG"
                     /translation="DYKDDDDK"
     CDS             7063..7173
                     /codon_start=1
                     /label=GS_XTEN_GS_linker
                     /translation="SGGSSGSGSETPGTSESATPESSGGSSSGGGSGGSGS"
     CDS             7174..7995
                     /codon_start=1
                     /label=SLX1A
                     /translation="GPAGVAARPGRFFGVYLLYCLNPRYRGRVYVGFTVNTARRVQQHN
                     GGRKKGGAWRTSGRGPWEMVLVVHGFPSSVAALRFEWAWQHPHASRRLAHVGPRLRGET
                     AFAFHLRVLAHMLRAPPWARLPLTLRWVRPDLRQDLCLPPPPHVPLAFGPPPPQAPAPR
                     RRAGPFDDAEPEPDQGDPGACCSLCAQTIQDEEGPLCCPHPGCLLRAHVICLAEEFLQE
                     EPGQLLPLEGQCPCCEKSLLWGDLIWLCQMDTEKEVEDSELEEAHWTDLLET"
     CDS             7996..8061
                     /codon_start=1
                     /label=P2A
                     /note="P2A"
                     /translation="GSGATNFSLLKQAGDVEENPGP"
     CDS             8062..8460
                     /codon_start=1
                     /label=Bla
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8483..9076
                     /label=WPRE; Woodchuck post-transcriptional
                     /note="WPRE; Woodchuck post-transcriptional regulatory 
                     element"
     LTR             9591..9779
                     /label=3' LTR
                     /note="3' LTR"
     rep_origin      12228..12453
                     /direction=RIGHT
                     /label=ColE1
                     /note="ColE1"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agctagctag gtcttgaaag gagtgggaat tggctccggt
     2641 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     2701 ggcaattgat ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     2761 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     2821 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggaccggtt ctagagcgct
     2881 gccaccatgg acaagaagta cagcatcggc ctggacatcg gcaccaactc tgtgggctgg
     2941 gccgtgatca ccgacgagta caaggtgccc agcaagaaat tcaaggtgct gggcaacacc
     3001 gaccggcaca gcatcaagaa gaacctgatc ggagccctgc tgttcgacag cggcgaaaca
     3061 gccgaggcca cccggctgaa gagaaccgcc agaagaagat acaccagacg gaagaaccgg
     3121 atctgctatc tgcaagagat cttcagcaac gagatggcca aggtggacga cagcttcttc
     3181 cacagactgg aagagtcctt cctggtggaa gaggataaga agcacgagcg gcaccccatc
     3241 ttcggcaaca tcgtggacga ggtggcctac cacgagaagt accccaccat ctaccacctg
     3301 agaaagaaac tggtggacag caccgacaag gccgacctgc ggctgatcta tctggccctg
     3361 gcccacatga tcaagttccg gggccacttc ctgatcgagg gcgacctgaa ccccgacaac
     3421 agcgacgtgg acaagctgtt catccagctg gtgcagacct acaaccagct gttcgaggaa
     3481 aaccccatca acgccagcgg cgtggacgcc aaggccatcc tgtctgccag actgagcaag
     3541 agcagacggc tggaaaatct gatcgcccag ctgcccggcg agaagaagaa tggcctgttc
     3601 ggaaacctga ttgccctgag cctgggcctg acccccaact tcaagagcaa cttcgacctg
     3661 gccgaggatg ccaaactgca gctgagcaag gacacctacg acgacgacct ggacaacctg
     3721 ctggcccaga tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac
     3781 gccatcctgc tgagcgacat cctgagagtg aacaccgaga tcaccaaggc ccccctgagc
     3841 gcctctatga tcaagagata cgacgagcac caccaggacc tgaccctgct gaaagctctc
     3901 gtgcggcagc agctgcctga gaagtacaaa gagattttct tcgaccagag caagaacggc
     3961 tacgccggct acattgacgg cggagccagc caggaagagt tctacaagtt catcaagccc
     4021 atcctggaaa agatggacgg caccgaggaa ctgctcgtga agctgaacag agaggacctg
     4081 ctgcggaagc agcggacctt cgacaacggc agcatccccc accagatcca cctgggagag
     4141 ctgcacgcca ttctgcggcg gcaggaagat ttttacccat tcctgaagga caaccgggaa
     4201 aagatcgaga agatcctgac cttccgcatc ccctactacg tgggccctct ggccagggga
     4261 aacagcagat tcgcctggat gaccagaaag agcgaggaaa ccatcacccc ctggaacttc
     4321 gaggaagtgg tggacaaggg cgcttccgcc cagagcttca tcgagcggat gaccaacttc
     4381 gataagaacc tgcccaacga gaaggtgctg cccaagcaca gcctgctgta cgagtacttc
     4441 accgtgtata acgagctgac caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc
     4501 ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc tgttcaagac caaccggaaa
     4561 gtgaccgtga agcagctgaa agaggactac ttcaagaaaa tcgagtgctt cgactccgtg
     4621 gaaatctccg gcgtggaaga tcggttcaac gcctccctgg gcacatacca cgatctgctg
     4681 aaaattatca aggacaagga cttcctggac aatgaggaaa acgaggacat tctggaagat
     4741 atcgtgctga ccctgacact gtttgaggac agagagatga tcgaggaacg gctgaaaacc
     4801 tatgcccacc tgttcgacga caaagtgatg aagcagctga agcggcggag atacaccggc
     4861 tggggcaggc tgagccggaa gctgatcaac ggcatccggg acaagcagtc cggcaagaca
     4921 atcctggatt tcctgaagtc cgacggcttc gccaacagaa acttcatgca gctgatccac
     4981 gacgacagcc tgacctttaa agaggacatc cagaaagccc aggtgtccgg ccagggcgat
     5041 agcctgcacg agcacattgc caatctggcc ggcagccccg ccattaagaa gggcatcctg
     5101 cagacagtga aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa gcccgagaac
     5161 atcgtgatcg aaatggccag agagaaccag accacccaga agggacagaa gaacagccgc
     5221 gagagaatga agcggatcga agagggcatc aaagagctgg gcagccagat cctgaaagaa
     5281 caccccgtgg aaaacaccca gctgcagaac gagaagctgt acctgtacta cctgcagaat
     5341 gggcgggata tgtacgtgga ccaggaactg gacatcaacc ggctgtccga ctacgatgtg
     5401 gaccatatcg tgcctcagag ctttctgaag gacgactcca tcgacaacaa ggtgctgacc
     5461 agaagcgaca agaaccgggg caagagcgac aacgtgccct ccgaagaggt cgtgaagaag
     5521 atgaagaact actggcggca gctgctgaac gccaagctga ttacccagag aaagttcgac
     5581 aatctgacca aggccgagag aggcggcctg agcgaactgg ataaggccgg cttcatcaag
     5641 agacagctgg tggaaacccg gcagatcaca aagcacgtgg cacagatcct ggactcccgg
     5701 atgaacacta agtacgacga gaatgacaag ctgatccggg aagtgaaagt gatcaccctg
     5761 aagtccaagc tggtgtccga tttccggaag gatttccagt tttacaaagt gcgcgagatc
     5821 aacaactacc accacgccca cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc
     5881 aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg actacaaggt gtacgacgtg
     5941 cggaagatga tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc
     6001 tacagcaaca tcatgaactt tttcaagacc gagattaccc tggccaacgg cgagatccgg
     6061 aagcggcctc tgatcgagac aaacggcgaa accggggaga tcgtgtggga taagggccgg
     6121 gattttgcca ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt gaaaaagacc
     6181 gaggtgcaga caggcggctt cagcaaagag tctatcctgc ccaagaggaa cagcgataag
     6241 ctgatcgcca gaaagaagga ctgggaccct aagaagtacg gcggcttcga cagccccacc
     6301 gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag
     6361 agtgtgaaag agctgctggg gatcaccatc atggaaagaa gcagcttcga gaagaatccc
     6421 atcgactttc tggaagccaa gggctacaaa gaagtgaaaa aggacctgat catcaagctg
     6481 cctaagtact ccctgttcga gctggaaaac ggccggaaga gaatgctggc ctctgccggc
     6541 gaactgcaga agggaaacga actggccctg ccctccaaat atgtgaactt cctgtacctg
     6601 gccagccact atgagaagct gaagggctcc cccgaggata atgagcagaa acagctgttt
     6661 gtggaacagc acaagcacta cctggacgag atcatcgagc agatcagcga gttctccaag
     6721 agagtgatcc tggccgacgc taatctggac aaagtgctgt ccgcctacaa caagcaccgg
     6781 gataagccca tcagagagca ggccgagaat atcatccacc tgtttaccct gaccaatctg
     6841 ggagcccctg ccgccttcaa gtactttgac accaccatcg accggaagag gtacaccagc
     6901 accaaagagg tgctggacgc caccctgatc caccagagca tcaccggcct gtacgagaca
     6961 cggatcgacc tgtctcagct gggaggcgac aagcgacctg ccgccacaaa gaaggctgga
     7021 caggctaaga agaagaaaga ttacaaagac gatgacgata agtctggagg atctagcgga
     7081 tctggaagcg agacaccagg aacaagcgag tcagcaacac cagagagcag tggcggcagc
     7141 agctcgggag gtggttcggg tggatctgga tcaggtcccg cgggggtcgc ggcgaggcca
     7201 gggcgctttt tcggcgtcta cctgctctac tgcctgaacc cccggtaccg gggccgcgtc
     7261 tacgtggggt tcactgtcaa cactgctcgt cgggtccagc agcacaatgg gggccgcaaa
     7321 aaaggcgggg cctggcggac cagcgggcga gggccctggg agatggtgct cgtcgtgcac
     7381 ggcttcccgt cctccgtggc cgcccttcgg tttgagtggg cttggcagca cccgcacgcc
     7441 tcgcgccgcc tggcgcacgt ggggcctcgc ctgcgaggag agacagcctt cgctttccac
     7501 ctgcgcgtgc tggcgcacat gctgcgcgca ccgccctggg ctcgcctccc gctcacgctg
     7561 cgctgggtgc gcccagacct ccgccaggac ctctgcctcc cgccgccgcc gcacgtgcct
     7621 ctggccttcg ggcctccacc gccccaggcc ccggccccaa ggcgccgcgc aggtcccttt
     7681 gatgacgcgg agcctgagcc agaccagggg gatccagggg cctgctgctc cctgtgcgcc
     7741 cagaccatcc aggatgaaga ggggcccttg tgttgccccc accctggctg cctgctaagg
     7801 gcccatgtga tctgcctggc agaggagttt cttcaggaag aaccagggca gcttctgccc
     7861 ctagagggcc aatgcccttg ctgtgagaag tcactgcttt ggggagacct gatctggctg
     7921 tgccagatgg acactgagaa agaagtagaa gactcagaat tagaagaggc acactggaca
     7981 gacctgctgg agaccggatc cggcgcaaca aacttctctc tgctgaaaca agccggagat
     8041 gtcgaagaga atcctggacc gatggccaag cctttgtctc aagaagaatc caccctcatt
     8101 gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc
     8161 gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg
     8221 ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg
     8281 acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc
     8341 cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga
     8401 cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa
     8461 gaattcgata tcaagcttat cggtaatcaa cctctggatt acaaaatttg tgaaagattg
     8521 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     8581 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     8641 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     8701 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     8761 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     8821 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     8881 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     8941 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     9001 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     9061 gccgcctccc cgcatcgata ccgtcgacct cgagacctag aaaaacatgg agcaatcaca
     9121 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag
     9181 gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
     9241 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     9301 cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg
     9361 cagaactaca caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag
     9421 ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg
     9481 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg
     9541 tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg
     9601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     9661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     9721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg
     9781 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     9841 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     9901 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     9961 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
    10021 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
    10081 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
    10141 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
    10201 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
    10261 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
    10321 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
    10381 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
    10441 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
    10501 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
    10561 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
    10621 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
    10681 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
    10741 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
    10801 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
    10861 ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat
    10921 agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca
    10981 gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc
    11041 ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg
    11101 tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg
    11161 tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact
    11221 tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt
    11281 tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac
    11341 acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
    11401 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
    11461 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
    11521 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
    11581 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
    11641 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
    11701 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
    11761 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
    11821 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
    11881 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
    11941 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    12001 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    12061 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    12121 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    12181 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    12241 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    12301 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    12361 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    12421 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    12481 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    12541 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    12601 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    12661 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    12721 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    12781 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    12841 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    12901 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    12961 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    13021 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    13081 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    13141 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    13201 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    13261 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    13321 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    13381 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    13441 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    13501 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    13561 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    13621 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    13681 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    13741 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gac
//
