LOCUS       Exported                9991 bp ds-DNA     circular SYN 08-JAN-2026
DEFINITION  Mammalian expression of SpCas9 PEmax prime editor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jan 14, 2026 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Imported from database: AddGene Entry: pCMV-PEmax URL: 
            https://www.addgene.org/browse/sequence/406249/ GENERAL Depositing 
            Lab: David Liu Publication: Chen et al Cell. 2021 Oct 
            28;184(22):5635-5652.e29. doi: 10.1016/j.cell.2021.09.018. Purpose: 
            Mammalian expression of SpCas9 PEmax prime editor BACKBONE Vector 
            backbone: pCMV Vector type: Mammalian Expression CLONING INFORMATION
            3′ sequencing primer: BGH-reverse 5′ sequencing primer: T7-forward 
            Cloning method: Ligation Independent Cloning GROWTH IN BACTERIA 
            Bacterial Resistance(s): Ampicillin, 100 ug/mL Copy number: High 
            Copy Growth Strain(s): NEB Stable Growth Temperature: 37(o)C 
            GENE/INSERT Alt name: SV40_bpNLS-SpCas9(R221K,N394K,H840A)-SGG 
            Sx2-SV40_bpNLS-SGGSx2-MMLV_RT(D200N, T306K, W313F, T330P, 
            L603W)-SV40_bpNLS-cMyc_NLS Gene/Insert name: PEmax Insert Size (bp):
            6393 Mutation: Detailed in manuscript Promoter: CMV Species: 
            Synthetic; SpCas9 is from Streptococcus pyogenes; MMLV_RT is from 
            the Moloney murine leukemia virus Tags / Fusion Proteins:  TERMS AND
            LICENSES Academic/Nonprofit Terms:  Industry Terms:
FEATURES             Location/Qualifiers
     source          1..9991
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     595..647
                     /label=prWB358_TE_mRNA_subclone_Gibson_F
     misc_feature    625..630
                     /label=Kozak
     CDS             631..4734
                     /codon_start=1
                     /label=hSpCas9
                     /note="hSpCas9"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     4679..4698
                     /label=prWB007_Cas9_XTEN_seq_F
     CDS             4735..4782
                     /codon_start=1
                     /label=NLS
                     /note="NLS"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4783..4806
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG"
                     /translation="DYKDDDDK"
     CDS             4807..4917
                     /codon_start=1
                     /label=GS_XTEN_GS_linker
                     /translation="SGGSSGSGSETPGTSESATPESSGGSSSGGGSGGSGS"
     primer_bind     4888..4934
                     /label=prWB061_BARD1_gibson_F
     CDS             4918..7245
                     /codon_start=1
                     /label=BARD1
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=99.6"
                     /note="/match_length=99.9"
                     /note="/other=CDS"
                     /translation="PDNRQPRNRQPRIRSGNEPRSASAMEPDGRGAWAHSRAALDRLEK
                     LLRCSRCTNILREPVCLGGCEHIFCSNCVSDCIGTGCPVCYTPAWIQDLKINRQLDSMI
                     QLCSKLRNLLHDNELSDLKEDKPRKSLFNDAGNKKNSIKMWFSPRSKKVRYVVSKASVQ
                     TQPAIKKDASAQQDSYEFVSPSPPADVSERAKKASARSGKKQKKKTLAEINQKWNLEAE
                     KEDGEFDSKEESKQKLVSFCSQPSVISSPQINGEIDLLASGSLTESECFGSLTEVSLPL
                     AEQIESPDTKSRNEVVTPEKVCKNYLTSKKSLPLENNGKRGHHNRLSSPISKRCRTSIL
                     STSGDFVKQTVPSENIPLPECSSPPSCKRKVGGTSGRKNSNMSDEFISLSPGTPPSTLS
                     SSSYRRVMSSPSAMKLLPNMAVKRNHRGETLLHIASIKGDIPSVEYLLQNGSDANVKDH
                     AGWTPLHEACNHGHLKVVELLLQHKALVNTTGYQNDSPLHDAAKNGHVDIVKLLLSYGA
                     SRNAVNIFGLRPVDYTDDESMKSLLLLPEKNESSSASHCSVMNTGQRRDGPLVLIGSGL
                     SSEQQKMLSELAVILKAKKYTEFDSTVTHVVVPGDAVQSTLKCMLGILNGCWILKFEWV
                     KACLRRKVCEQEEKYEIPEGPRRSRLNREQLLPKLFDGCYFYLWGTFKHHPKDNLIKLV
                     TAGGGQILSRKPKPDSDVTQTINTVAYHARPDSDQRFCTQYIIYEDLCNYHPERVRQGK
                     VWKAPSSWFIDCVMSFELLPLDS"
     primer_bind     complement(7221..7275)
                     /label=prWB360_BARD1_mRNA_subclone_Gibson_R
     CDS             7255..7272
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7295..7312)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7301..7525
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7596..7612)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7596..7612)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7609..7631)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7620..7636
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        7644..7674
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7689..7710
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7827..7844)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7998..8586)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8078..8097)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8757..9617)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, 
                     andrelated antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9380..9399
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        9618..9722
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9801..9820)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      601 agatccgcta gagatccgcg gccggccacc atggacaaga agtacagcat cggcctggac
      661 atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt gcccagcaag
      721 aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct gatcggagcc
      781 ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac cgccagaaga
      841 agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag caacgagatg
      901 gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt ggaagaggat
      961 aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc ctaccacgag
     1021 aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga caaggccgac
     1081 ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca cttcctgatc
     1141 gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca gctggtgcag
     1201 acctacaacc agctgttcga ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc
     1261 atcctgtctg ccagactgag caagagcaga cggctggaaa atctgatcgc ccagctgccc
     1321 ggcgagaaga agaatggcct gttcggaaac ctgattgccc tgagcctggg cctgaccccc
     1381 aacttcaaga gcaacttcga cctggccgag gatgccaaac tgcagctgag caaggacacc
     1441 tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc cgacctgttt
     1501 ctggccgcca agaacctgtc cgacgccatc ctgctgagcg acatcctgag agtgaacacc
     1561 gagatcacca aggcccccct gagcgcctct atgatcaaga gatacgacga gcaccaccag
     1621 gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta caaagagatt
     1681 ttcttcgacc agagcaagaa cggctacgcc ggctacattg acggcggagc cagccaggaa
     1741 gagttctaca agttcatcaa gcccatcctg gaaaagatgg acggcaccga ggaactgctc
     1801 gtgaagctga acagagagga cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc
     1861 ccccaccaga tccacctggg agagctgcac gccattctgc ggcggcagga agatttttac
     1921 ccattcctga aggacaaccg ggaaaagatc gagaagatcc tgaccttccg catcccctac
     1981 tacgtgggcc ctctggccag gggaaacagc agattcgcct ggatgaccag aaagagcgag
     2041 gaaaccatca ccccctggaa cttcgaggaa gtggtggaca agggcgcttc cgcccagagc
     2101 ttcatcgagc ggatgaccaa cttcgataag aacctgccca acgagaaggt gctgcccaag
     2161 cacagcctgc tgtacgagta cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg
     2221 accgagggaa tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc catcgtggac
     2281 ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag
     2341 aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc
     2401 ctgggcacat accacgatct gctgaaaatt atcaaggaca aggacttcct ggacaatgag
     2461 gaaaacgagg acattctgga agatatcgtg ctgaccctga cactgtttga ggacagagag
     2521 atgatcgagg aacggctgaa aacctatgcc cacctgttcg acgacaaagt gatgaagcag
     2581 ctgaagcggc ggagatacac cggctggggc aggctgagcc ggaagctgat caacggcatc
     2641 cgggacaagc agtccggcaa gacaatcctg gatttcctga agtccgacgg cttcgccaac
     2701 agaaacttca tgcagctgat ccacgacgac agcctgacct ttaaagagga catccagaaa
     2761 gcccaggtgt ccggccaggg cgatagcctg cacgagcaca ttgccaatct ggccggcagc
     2821 cccgccatta agaagggcat cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg
     2881 atgggccggc acaagcccga gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc
     2941 cagaagggac agaagaacag ccgcgagaga atgaagcgga tcgaagaggg catcaaagag
     3001 ctgggcagcc agatcctgaa agaacacccc gtggaaaaca cccagctgca gaacgagaag
     3061 ctgtacctgt actacctgca gaatgggcgg gatatgtacg tggaccagga actggacatc
     3121 aaccggctgt ccgactacga tgtggaccat atcgtgcctc agagctttct gaaggacgac
     3181 tccatcgaca acaaggtgct gaccagaagc gacaagaacc ggggcaagag cgacaacgtg
     3241 ccctccgaag aggtcgtgaa gaagatgaag aactactggc ggcagctgct gaacgccaag
     3301 ctgattaccc agagaaagtt cgacaatctg accaaggccg agagaggcgg cctgagcgaa
     3361 ctggataagg ccggcttcat caagagacag ctggtggaaa cccggcagat cacaaagcac
     3421 gtggcacaga tcctggactc ccggatgaac actaagtacg acgagaatga caagctgatc
     3481 cgggaagtga aagtgatcac cctgaagtcc aagctggtgt ccgatttccg gaaggatttc
     3541 cagttttaca aagtgcgcga gatcaacaac taccaccacg cccacgacgc ctacctgaac
     3601 gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc tggaaagcga gttcgtgtac
     3661 ggcgactaca aggtgtacga cgtgcggaag atgatcgcca agagcgagca ggaaatcggc
     3721 aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa gaccgagatt
     3781 accctggcca acggcgagat ccggaagcgg cctctgatcg agacaaacgg cgaaaccggg
     3841 gagatcgtgt gggataaggg ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc
     3901 caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa agagtctatc
     3961 ctgcccaaga ggaacagcga taagctgatc gccagaaaga aggactggga ccctaagaag
     4021 tacggcggct tcgacagccc caccgtggcc tattctgtgc tggtggtggc caaagtggaa
     4081 aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac catcatggaa
     4141 agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta caaagaagtg
     4201 aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga aaacggccgg
     4261 aagagaatgc tggcctctgc cggcgaactg cagaagggaa acgaactggc cctgccctcc
     4321 aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg ctcccccgag
     4381 gataatgagc agaaacagct gtttgtggaa cagcacaagc actacctgga cgagatcatc
     4441 gagcagatca gcgagttctc caagagagtg atcctggccg acgctaatct ggacaaagtg
     4501 ctgtccgcct acaacaagca ccgggataag cccatcagag agcaggccga gaatatcatc
     4561 cacctgttta ccctgaccaa tctgggagcc cctgccgcct tcaagtactt tgacaccacc
     4621 atcgaccgga agaggtacac cagcaccaaa gaggtgctgg acgccaccct gatccaccag
     4681 agcatcaccg gcctgtacga gacacggatc gacctgtctc agctgggagg cgacaagcga
     4741 cctgccgcca caaagaaggc tggacaggct aagaagaaga aagattacaa agacgatgac
     4801 gataagtctg gaggatctag cggatctgga agcgagacac caggaacaag cgagtcagca
     4861 acaccagaga gcagtggcgg cagcagctcg ggaggtggtt cgggtggatc tggatcaccg
     4921 gataatcggc agccgaggaa ccggcagccg aggatccgct ccgggaacga gcctcgttcc
     4981 gcgtccgcca tggaaccgga tggtcgcggt gcctgggccc acagtcgcgc cgcgctcgac
     5041 cgcctggaga agctgctgcg ctgctcgcgt tgtactaaca ttctgagaga gcctgtgtgt
     5101 ttaggaggat gtgagcacat cttctgtagt aattgtgtaa gtgactgcat tggaactgga
     5161 tgtccagtgt gttacacccc ggcctggata caagacttga agataaatag acaactggac
     5221 agcatgattc aactttgtag taagcttcga aatttgctac atgacaatga gctgtcagat
     5281 ttgaaagaag ataaacctag gaaaagtttg tttaatgatg caggaaacaa gaagaattca
     5341 attaaaatgt ggtttagccc tcgaagtaag aaagtcagat atgttgtgag taaagcttca
     5401 gtgcaaaccc agcctgcaat aaaaaaagat gcaagtgctc agcaagactc atatgaattt
     5461 gtttccccaa gtcctcctgc agatgtttct gagagggcta aaaaggcttc tgcaagatct
     5521 ggaaaaaagc aaaaaaagaa aactttagct gaaatcaacc aaaaatggaa tttagaggca
     5581 gaaaaagaag atggtgaatt tgactccaaa gaggaatcta agcaaaagct ggtatccttc
     5641 tgtagccaac catctgttat ctccagtcct cagataaatg gtgaaataga cttactagca
     5701 agtggctcct tgacagaatc tgaatgtttt ggaagtttaa ctgaagtctc tttaccattg
     5761 gctgagcaaa tagagtctcc agacactaag agcaggaatg aagtagtgac tcctgagaag
     5821 gtctgcaaaa attatcttac atctaagaaa tctttgccat tagaaaataa tggaaaacgt
     5881 ggccatcaca atagactttc cagtcccatt tctaagagat gtagaaccag cattctgagc
     5941 accagtggag attttgttaa gcaaacggtg ccctcagaaa atataccatt gcctgaatgt
     6001 tcttcaccac cttcatgcaa acgtaaagtt ggtggtacat cagggaggaa aaacagtaac
     6061 atgtccgatg aattcattag tctttcacca ggtacaccac cttctacatt aagtagttca
     6121 agttacaggc gagtgatgtc tagtccctca gcaatgaagc tgttgcccaa tatggctgtg
     6181 aaaagaaatc atagaggaga gactttgctc catattgctt ctattaaggg cgacatacct
     6241 tctgttgaat accttttaca aaatggaagt gatgcaaatg ttaaagacca tgctggatgg
     6301 acaccattgc atgaagcttg caatcatggg cacctgaagg tagtggaatt attgctccag
     6361 cataaggcat tggtgaacac caccgggtat caaaatgact caccacttca cgatgcagcc
     6421 aagaatgggc acgtggatat agtcaagctg ttactttcct atggagcctc cagaaatgct
     6481 gttaatatat ttggtctgcg gcctgtcgat tatacagatg atgaaagtat gaaatcgcta
     6541 ttgctgctac cagagaagaa tgaatcatcc tcagctagcc actgctcagt aatgaacact
     6601 gggcagcgta gggatggacc tcttgtactt ataggcagtg ggctgtcttc agaacaacag
     6661 aaaatgctca gtgagcttgc agtaattctt aaggctaaaa aatatactga gtttgacagt
     6721 acagtaactc atgttgttgt tcctggtgat gcagttcaaa gtaccttgaa gtgtatgctt
     6781 gggattctca atggatgctg gattctaaaa tttgaatggg taaaagcatg tctacgaaga
     6841 aaagtatgtg aacaggaaga aaagtatgaa attcctgaag gtccacgcag aagcaggctc
     6901 aacagagaac agctgttgcc aaagctgttt gatggatgct acttctattt gtggggaacc
     6961 ttcaaacacc atccaaagga caaccttatt aagctcgtca ctgcaggtgg gggccagatc
     7021 ctcagtagaa agcccaagcc agacagtgac gtgactcaga ccatcaatac agtcgcatac
     7081 catgcgagac ccgattctga tcagcgcttc tgcacacagt atatcatcta tgaagatttg
     7141 tgtaattatc acccagagag ggttcggcag ggcaaagtct ggaaggctcc ttcgagctgg
     7201 tttatagact gtgtgatgtc ctttgagttg cttcctcttg acagctagac cggtcatcat
     7261 caccatcacc attgagttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     7321 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     7381 gtcctttcct aataaaatga gaaaattgca tcgcattgtc tgagtaggtg tcattctatt
     7441 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     7501 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctcgata
     7561 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     7621 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctag
     7681 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     7741 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     7801 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     7861 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7921 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7981 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     8041 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     8101 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     8161 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     8221 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     8281 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     8341 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     8401 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     8461 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     8521 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     8581 tctcaagaag atcctttgat cttttctacg gggtctgaca ctcagtggaa cgaaaactca
     8641 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     8701 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     8761 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     8821 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     8881 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     8941 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     9001 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     9061 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     9121 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     9181 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     9241 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     9301 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     9361 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     9421 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     9481 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     9541 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     9601 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     9661 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     9721 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatcg atctcccgat
     9781 cccctagggt cgactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
     9841 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
     9901 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
     9961 cttcgcgatg tacgggccag atatacgcgt t
//
