LOCUS       Exported                8489 bp ds-DNA     circular SYN 08-JAN-2026
DEFINITION  Mammalian expression of SpCas9 PEmax prime editor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8489)
  AUTHORS   Wells Burrell
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jan 14, 2026 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Imported from database: AddGene Entry: pCMV-PEmax URL: 
            https://www.addgene.org/browse/sequence/406249/ GENERAL Depositing 
            Lab: David Liu Publication: Chen et al Cell. 2021 Oct 
            28;184(22):5635-5652.e29. doi: 10.1016/j.cell.2021.09.018. Purpose: 
            Mammalian expression of SpCas9 PEmax prime editor BACKBONE Vector 
            backbone: pCMV Vector type: Mammalian Expression CLONING INFORMATION
            3′ sequencing primer: BGH-reverse 5′ sequencing primer: T7-forward 
            Cloning method: Ligation Independent Cloning GROWTH IN BACTERIA 
            Bacterial Resistance(s): Ampicillin, 100 ug/mL Copy number: High 
            Copy Growth Strain(s): NEB Stable Growth Temperature: 37(o)C 
            GENE/INSERT Alt name: SV40_bpNLS-SpCas9(R221K,N394K,H840A)-SGG 
            Sx2-SV40_bpNLS-SGGSx2-MMLV_RT(D200N, T306K, W313F, T330P, 
            L603W)-SV40_bpNLS-cMyc_NLS Gene/Insert name: PEmax Insert Size (bp):
            6393 Mutation: Detailed in manuscript Promoter: CMV Species: 
            Synthetic; SpCas9 is from Streptococcus pyogenes; MMLV_RT is from 
            the Moloney murine leukemia virus Tags / Fusion Proteins:  TERMS AND
            LICENSES Academic/Nonprofit Terms:  Industry Terms:
FEATURES             Location/Qualifiers
     source          1..8489
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    626..631
                     /label=Kozak
     CDS             632..4735
                     /codon_start=1
                     /label=hSpCas9
                     /note="hSpCas9"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4736..4783
                     /codon_start=1
                     /label=NLS
                     /note="NLS"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4784..4807
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG"
                     /translation="DYKDDDDK"
     CDS             4808..4918
                     /codon_start=1
                     /label=GS_XTEN_GS_linker
                     /translation="SGGSSGSGSETPGTSESATPESSGGSSSGGGSGGSGS"
     CDS             4919..5740
                     /codon_start=1
                     /label=SLX1A
                     /translation="GPAGVAARPGRFFGVYLLYCLNPRYRGRVYVGFTVNTARRVQQHN
                     GGRKKGGAWRTSGRGPWEMVLVVHGFPSSVAALRFEWAWQHPHASRRLAHVGPRLRGET
                     AFAFHLRVLAHMLRAPPWARLPLTLRWVRPDLRQDLCLPPPPHVPLAFGPPPPQAPAPR
                     RRAGPFDDAEPEPDQGDPGACCSLCAQTIQDEEGPLCCPHPGCLLRAHVICLAEEFLQE
                     EPGQLLPLEGQCPCCEKSLLWGDLIWLCQMDTEKEVEDSELEEAHWTDLLET"
     CDS             5741..5743
                     /codon_start=1
                     /label=StopCodon
                     /translation=""
     primer_bind     complement(5793..5810)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5799..6023
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6094..6110)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6094..6110)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6107..6129)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6118..6134
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        6142..6172
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6187..6208
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6325..6342)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6496..7084)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6576..6595)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7255..8115)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, 
                     andrelated antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7878..7897
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        8116..8220
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8299..8318)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      601 agatccgcta gagatccgcg gccgcgccac catggacaag aagtacagca tcggcctgga
      661 catcggcacc aactctgtgg gctgggccgt gatcaccgac gagtacaagg tgcccagcaa
      721 gaaattcaag gtgctgggca acaccgaccg gcacagcatc aagaagaacc tgatcggagc
      781 cctgctgttc gacagcggcg aaacagccga ggccacccgg ctgaagagaa ccgccagaag
      841 aagatacacc agacggaaga accggatctg ctatctgcaa gagatcttca gcaacgagat
      901 ggccaaggtg gacgacagct tcttccacag actggaagag tccttcctgg tggaagagga
      961 taagaagcac gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg cctaccacga
     1021 gaagtacccc accatctacc acctgagaaa gaaactggtg gacagcaccg acaaggccga
     1081 cctgcggctg atctatctgg ccctggccca catgatcaag ttccggggcc acttcctgat
     1141 cgagggcgac ctgaaccccg acaacagcga cgtggacaag ctgttcatcc agctggtgca
     1201 gacctacaac cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc
     1261 catcctgtct gccagactga gcaagagcag acggctggaa aatctgatcg cccagctgcc
     1321 cggcgagaag aagaatggcc tgttcggaaa cctgattgcc ctgagcctgg gcctgacccc
     1381 caacttcaag agcaacttcg acctggccga ggatgccaaa ctgcagctga gcaaggacac
     1441 ctacgacgac gacctggaca acctgctggc ccagatcggc gaccagtacg ccgacctgtt
     1501 tctggccgcc aagaacctgt ccgacgccat cctgctgagc gacatcctga gagtgaacac
     1561 cgagatcacc aaggcccccc tgagcgcctc tatgatcaag agatacgacg agcaccacca
     1621 ggacctgacc ctgctgaaag ctctcgtgcg gcagcagctg cctgagaagt acaaagagat
     1681 tttcttcgac cagagcaaga acggctacgc cggctacatt gacggcggag ccagccagga
     1741 agagttctac aagttcatca agcccatcct ggaaaagatg gacggcaccg aggaactgct
     1801 cgtgaagctg aacagagagg acctgctgcg gaagcagcgg accttcgaca acggcagcat
     1861 cccccaccag atccacctgg gagagctgca cgccattctg cggcggcagg aagattttta
     1921 cccattcctg aaggacaacc gggaaaagat cgagaagatc ctgaccttcc gcatccccta
     1981 ctacgtgggc cctctggcca ggggaaacag cagattcgcc tggatgacca gaaagagcga
     2041 ggaaaccatc accccctgga acttcgagga agtggtggac aagggcgctt ccgcccagag
     2101 cttcatcgag cggatgacca acttcgataa gaacctgccc aacgagaagg tgctgcccaa
     2161 gcacagcctg ctgtacgagt acttcaccgt gtataacgag ctgaccaaag tgaaatacgt
     2221 gaccgaggga atgagaaagc ccgccttcct gagcggcgag cagaaaaagg ccatcgtgga
     2281 cctgctgttc aagaccaacc ggaaagtgac cgtgaagcag ctgaaagagg actacttcaa
     2341 gaaaatcgag tgcttcgact ccgtggaaat ctccggcgtg gaagatcggt tcaacgcctc
     2401 cctgggcaca taccacgatc tgctgaaaat tatcaaggac aaggacttcc tggacaatga
     2461 ggaaaacgag gacattctgg aagatatcgt gctgaccctg acactgtttg aggacagaga
     2521 gatgatcgag gaacggctga aaacctatgc ccacctgttc gacgacaaag tgatgaagca
     2581 gctgaagcgg cggagataca ccggctgggg caggctgagc cggaagctga tcaacggcat
     2641 ccgggacaag cagtccggca agacaatcct ggatttcctg aagtccgacg gcttcgccaa
     2701 cagaaacttc atgcagctga tccacgacga cagcctgacc tttaaagagg acatccagaa
     2761 agcccaggtg tccggccagg gcgatagcct gcacgagcac attgccaatc tggccggcag
     2821 ccccgccatt aagaagggca tcctgcagac agtgaaggtg gtggacgagc tcgtgaaagt
     2881 gatgggccgg cacaagcccg agaacatcgt gatcgaaatg gccagagaga accagaccac
     2941 ccagaaggga cagaagaaca gccgcgagag aatgaagcgg atcgaagagg gcatcaaaga
     3001 gctgggcagc cagatcctga aagaacaccc cgtggaaaac acccagctgc agaacgagaa
     3061 gctgtacctg tactacctgc agaatgggcg ggatatgtac gtggaccagg aactggacat
     3121 caaccggctg tccgactacg atgtggacca tatcgtgcct cagagctttc tgaaggacga
     3181 ctccatcgac aacaaggtgc tgaccagaag cgacaagaac cggggcaaga gcgacaacgt
     3241 gccctccgaa gaggtcgtga agaagatgaa gaactactgg cggcagctgc tgaacgccaa
     3301 gctgattacc cagagaaagt tcgacaatct gaccaaggcc gagagaggcg gcctgagcga
     3361 actggataag gccggcttca tcaagagaca gctggtggaa acccggcaga tcacaaagca
     3421 cgtggcacag atcctggact cccggatgaa cactaagtac gacgagaatg acaagctgat
     3481 ccgggaagtg aaagtgatca ccctgaagtc caagctggtg tccgatttcc ggaaggattt
     3541 ccagttttac aaagtgcgcg agatcaacaa ctaccaccac gcccacgacg cctacctgaa
     3601 cgccgtcgtg ggaaccgccc tgatcaaaaa gtaccctaag ctggaaagcg agttcgtgta
     3661 cggcgactac aaggtgtacg acgtgcggaa gatgatcgcc aagagcgagc aggaaatcgg
     3721 caaggctacc gccaagtact tcttctacag caacatcatg aactttttca agaccgagat
     3781 taccctggcc aacggcgaga tccggaagcg gcctctgatc gagacaaacg gcgaaaccgg
     3841 ggagatcgtg tgggataagg gccgggattt tgccaccgtg cggaaagtgc tgagcatgcc
     3901 ccaagtgaat atcgtgaaaa agaccgaggt gcagacaggc ggcttcagca aagagtctat
     3961 cctgcccaag aggaacagcg ataagctgat cgccagaaag aaggactggg accctaagaa
     4021 gtacggcggc ttcgacagcc ccaccgtggc ctattctgtg ctggtggtgg ccaaagtgga
     4081 aaagggcaag tccaagaaac tgaagagtgt gaaagagctg ctggggatca ccatcatgga
     4141 aagaagcagc ttcgagaaga atcccatcga ctttctggaa gccaagggct acaaagaagt
     4201 gaaaaaggac ctgatcatca agctgcctaa gtactccctg ttcgagctgg aaaacggccg
     4261 gaagagaatg ctggcctctg ccggcgaact gcagaaggga aacgaactgg ccctgccctc
     4321 caaatatgtg aacttcctgt acctggccag ccactatgag aagctgaagg gctcccccga
     4381 ggataatgag cagaaacagc tgtttgtgga acagcacaag cactacctgg acgagatcat
     4441 cgagcagatc agcgagttct ccaagagagt gatcctggcc gacgctaatc tggacaaagt
     4501 gctgtccgcc tacaacaagc accgggataa gcccatcaga gagcaggccg agaatatcat
     4561 ccacctgttt accctgacca atctgggagc ccctgccgcc ttcaagtact ttgacaccac
     4621 catcgaccgg aagaggtaca ccagcaccaa agaggtgctg gacgccaccc tgatccacca
     4681 gagcatcacc ggcctgtacg agacacggat cgacctgtct cagctgggag gcgacaagcg
     4741 acctgccgcc acaaagaagg ctggacaggc taagaagaag aaagattaca aagacgatga
     4801 cgataagtct ggaggatcta gcggatctgg aagcgagaca ccaggaacaa gcgagtcagc
     4861 aacaccagag agcagtggcg gcagcagctc gggaggtggt tcgggtggat ctggatcagg
     4921 tcccgcgggg gtcgcggcga ggccagggcg ctttttcggc gtctacctgc tctactgcct
     4981 gaacccccgg taccggggcc gcgtctacgt ggggttcact gtcaacactg ctcgtcgggt
     5041 ccagcagcac aatgggggcc gcaaaaaagg cggggcctgg cggaccagcg ggcgagggcc
     5101 ctgggagatg gtgctcgtcg tgcacggctt cccgtcctcc gtggccgccc ttcggtttga
     5161 gtgggcttgg cagcacccgc acgcctcgcg ccgcctggcg cacgtggggc ctcgcctgcg
     5221 aggagagaca gccttcgctt tccacctgcg cgtgctggcg cacatgctgc gcgcaccgcc
     5281 ctgggctcgc ctcccgctca cgctgcgctg ggtgcgccca gacctccgcc aggacctctg
     5341 cctcccgccg ccgccgcacg tgcctctggc cttcgggcct ccaccgcccc aggccccggc
     5401 cccaaggcgc cgcgcaggtc cctttgatga cgcggagcct gagccagacc agggggatcc
     5461 aggggcctgc tgctccctgt gcgcccagac catccaggat gaagaggggc ccttgtgttg
     5521 cccccaccct ggctgcctgc taagggccca tgtgatctgc ctggcagagg agtttcttca
     5581 ggaagaacca gggcagcttc tgcccctaga gggccaatgc ccttgctgtg agaagtcact
     5641 gctttgggga gacctgatct ggctgtgcca gatggacact gagaaagaag tagaagactc
     5701 agaattagaa gaggcacact ggacagacct gctggagacc tagtagaccg gtcatcatca
     5761 ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     5821 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     5881 cctttcctaa taaaatgaga aaattgcatc gcattgtctg agtaggtgtc attctattct
     5941 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     6001 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgatacc
     6061 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     6121 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctaggg
     6181 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     6241 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     6301 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6361 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6421 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6481 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6541 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6601 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6661 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6721 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6781 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     6841 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     6901 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     6961 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7021 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7081 tcaagaagat cctttgatct tttctacggg gtctgacact cagtggaacg aaaactcacg
     7141 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7201 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     7261 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     7321 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     7381 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     7441 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     7501 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     7561 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     7621 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     7681 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     7741 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     7801 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     7861 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     7921 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     7981 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     8041 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     8101 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     8161 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     8221 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcgat ctcccgatcc
     8281 cctagggtcg actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     8341 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
     8401 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
     8461 tcgcgatgta cgggccagat atacgcgtt
//
