LOCUS       Exported                6495 bp ds-DNA     circular SYN 23-FEB-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6495)
  AUTHORS   Feng Luo
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Feb 23, 2026 from SnapGene 4.3.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6495
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     CDS             1..49
                     /label=lacI
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366; direction: RIGHT"
     rep_origin      138..726
                     /direction=RIGHT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
     terminator      951..968
                     /label=rrnB T2 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=64.3"
                     /note="/other=terminator"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             complement(993..1012)
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=11.5"
                     /note="/other=CDS"
                     /note="color: #993366; direction: LEFT"
     primer_bind     996..1012
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        1475..1493
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    1494..1518
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
                     /note="color: #31849b; direction: RIGHT"
     RBS             1546..1554
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
                     /note="color: #a6acb3"
     CDS             1566..1589
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHHHH"
     CDS             1590..1610
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /note="color: #cc99b2
                     Cleavage site after base 1607"
                     /translation="ENLYFQG"
     CDS             1590..1606
                     /label=TEV site (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=81.0"
                     /note="/other=CDS"
                     /note="color: #993366; direction: RIGHT"
     CDS             1614..4064
                     /codon_start=1
                     /label=Delta6
                     /note="color: #00ffff"
                     /translation="RSDLAVRSGIEALETAVEMAIAAMEAGLDPKEASKIVIELSREIV
                     EVVYKSKPDGEEMFEVAKAIADASLRMVEAMVDAGAPPRVIAEAIARGAKVISEAVIKA
                     VELGAPLELVKEAAEYGLEVLREAAELARKRGGDPDTLEAVLEAAKASSELIIMVAEGE
                     PPEVVAERAIEALKRLTEMAIEAMKKGGDPEVAIEVVANAAESALKAVAAAGPPPEVME
                     RMIKAANEVMQRMVQAAIEAGADGKVLKKAIKKAAEVSVKGIRLAVELVARSDLAVRSG
                     IDALETAVEMAIAAMEAGLDPKEASKIVIELSREIVEVVYKSKPDGEEMFEVAKAIADA
                     SLRMVEAMVDAGAPPRVIAEAIARGAKVISEAVIKAVELGAPLELVKEAAEYGLEVLRE
                     AAELARKRGGDPDTLEAVLEAAKASSELIIMVAEGEPPEVVAERAIEALKRLTEMAIEA
                     MKKGGDPEVAIEVVANAAESALKAVAAAGPPPEVMERMIKAANEVMQRMVQAAIEAGAD
                     GKVLKKAIKKAAEVSVKGIRLAVEGGLAVELVARSGIDALETAVEMAIAAMEAGLDPKE
                     ASKIVIELSREIVEVVYKSKPDGEEMFEVAKAIADASLRMVEAMVDAGAPPRVIAEAIA
                     RGAKVISEAVIKAVELGAPLELVKEAAEYGLEVLREAAELARKRGGDPDTLEAVLEAAK
                     ASSELIIMVAEGEPPEVVAERAIEALKRLTEMAIEAMKKGGDPEVAIEVVANAAESALK
                     AVAAAGPPPEVMERMIKAANEVMQRMVQAAIEAGADGKVLKKAIKKAAEVSVKGIRLAV
                     ENSPG"
     terminator      4074..4121
                     /label=T7 terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
                     /note="color: #ffffff; direction: RIGHT"
     terminator      4181..4221
                     /label=rrnB T1 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=87.2"
                     /note="/other=terminator"
                     /note="color: #ffffff"
     ncRNA           complement(4225..4340)
                     /label=5S rRNA
                     /note="pLannotate"
                     /note="/database=Rfam"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=97.5"
                     /note="/other=ncRNA"
                     /note="color: #8fbc8f; direction: LEFT"
     CDS             4369..4393
                     /label=KanR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.1"
                     /note="/other=CDS"
                     /note="color: #993366; direction: RIGHT"
     promoter        4430..4521
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             4522..5382
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.2"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366; direction: RIGHT"
     promoter        5391..5464
                     /label=lacI promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=94.9"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             5465..6495
                     /label=lacI
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366; direction: RIGHT"
ORIGIN
        1 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gactcatgac
       61 caaaatccct taacgtgagt tacgcgcgcg tcgttccact gagcgtcaga ccccgtagaa
      121 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
      181 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
      241 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
      301 tagttagccc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
      361 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
      421 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
      481 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
      541 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
      601 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
      661 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
      721 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
      781 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
      841 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
      901 gcggaaggcg agagtaggga actgccaggc atcaaactaa gcagaaggcc cctgacggat
      961 ggcctttttg cgtttctaca aactctttct gtgttgtaaa acgacggcca gtcttaagct
     1021 cgggccccct gggcggttct gataacgagt aatcgttaat ccgcaaataa cgtaaaaacc
     1081 cgcttcggcg ggttttttta tggggggagt ttagggaaag agcatttgtc agaatattta
     1141 agggcgcctg tcactttgct tgatatatga gaattattta accttataaa tgagaaaaaa
     1201 gcaacgcact ttaaataaga tacgttgctt tttcgattga tgaacaccta taattaaact
     1261 attcatctat tatttatgat tttttgtata tacaatattt ctagtttgtt aaagagaatt
     1321 aagaaaataa atctcgaaaa taataaaggg aaaatcagtt tttgatatca aaattataca
     1381 tgtcaacgat aatacaaaat ataatacaaa ctataagatg ttatcagtat ttattatcat
     1441 ttagaataaa ttttgtgtcg cccttccgcg aaattaatac gactcactat aggggaattg
     1501 tgagcggata acaattcccc tctagaaata attttgttta acttttaagg aggtaaaaaa
     1561 tgtcgcatca ccaccatcat caccatcacg aaaacctgta ttttcaagga tcccgcagcg
     1621 atctggccgt acgttctgga attgaagcct tagaaactgc agtggagatg gccatcgctg
     1681 ctatggaagc cggtctggac ccaaaagaag cgagtaaaat cgttattgaa cttagtcgcg
     1741 aaatcgtaga ggtcgtttac aagtccaaac cggacggaga agagatgttc gaagttgcaa
     1801 aagctatcgc ggatgcttct cttcgcatgg ttgaagctat ggtcgatgcc ggggcacccc
     1861 cgcgtgtaat tgctgaagct atcgctcgcg gagccaaggt gatttcggag gctgttatca
     1921 aggcggtaga attgggggca ccgttggagc ttgtaaaaga agccgcagaa tatggccttg
     1981 aggtacttcg cgaagctgca gagttagcac gcaagcgtgg gggtgaccca gacactctgg
     2041 aggctgtgct ggaagctgcg aaagcaagta gcgaacttat tatcatggtt gctgaagggg
     2101 aacccccaga agttgttgcg gaacgcgcca tcgaggctct gaaacgcttg actgaaatgg
     2161 ctattgaagc tatgaaaaag ggcggcgacc cagaggttgc aatcgaggtg gtagctaatg
     2221 cagcagaaag cgcattaaaa gctgtggcag cggcaggacc gccccccgag gtaatggaac
     2281 gtatgattaa ggctgcgaac gaagttatgc aacgtatggt tcaggcggcc attgaagcgg
     2341 gggctgacgg aaaagtgctg aaaaaggcaa ttaaaaaagc tgctgaggtt agtgttaagg
     2401 gcattcgcct tgcagttgag cttgtggcgc gctccgatct tgcagtccgg tctggcattg
     2461 atgcgctcga aacggctgtc gagatggcaa tagctgcgat ggaagctggg ttggatccca
     2521 aagaggcttc aaaaatcgta atcgaactta gtagggagat tgtagaggtt gtctataaat
     2581 caaaacctga cggtgaggag atgttcgagg tagcaaaagc gatagcagac gcatcacttc
     2641 ggatggtcga agcgatggta gatgcgggag caccccccag ggtcatagcc gaagcgattg
     2701 cgcgcggtgc taaggtgata tctgaagctg taatcaaagc ggtggaactg ggagcaccct
     2761 tggaactcgt aaaagaagca gccgaatatg gactcgaagt ccttcgcgag gccgcggaac
     2821 ttgctagaaa gcgcggagga gaccccgata ctcttgaggc agtactggag gccgctaagg
     2881 catctagtga actgattatt atggtagctg agggtgaacc ccccgaggtt gtagccgaga
     2941 gggccataga agctttgaag agattgactg agatggcgat tgaagctatg aagaagggcg
     3001 gcgaccctga agtcgcgatc gaggttgttg caaacgccgc cgaatctgca ctgaaggctg
     3061 tcgctgcggc tggcccaccc ccggaggtaa tggagcgcat gatcaaggcg gccaacgaag
     3121 tcatgcagcg catggtacaa gcagcaattg aagctggcgc ggatgggaag gtgttgaaaa
     3181 aagccatcaa aaaggctgcg gaggtaagtg ttaagggtat aagactcgcg gtagagggcg
     3241 ggttggctgt tgaactggta gcacgatctg gtatagatgc tcttgagact gcagttgaaa
     3301 tggcaatagc agcgatggaa gctggtctcg atcctaaaga agccagcaaa atagtaatcg
     3361 aactgtccag ggaaatagta gaagtagtat ataagtctaa accggacggt gaagaaatgt
     3421 ttgaagtagc taaagctata gcggacgcgt cccttcgaat ggtggaggct atggtagacg
     3481 cgggcgcacc gccacgcgta atcgcagagg ccatcgcccg cggggcgaag gttatttctg
     3541 aggcggtcat aaaagcggtc gagttgggtg cacctcttga gctcgttaag gaagcggccg
     3601 agtacggact ggaagtgttg agagaagccg cggaattggc ccgaaaacgc gggggagatc
     3661 ctgatacttt ggaggcggtg ttggaggccg cgaaggcgtc aagcgaattg atcatcatgg
     3721 tcgccgaggg ggagccccca gaagttgtcg cggagcgggc tattgaggcg ttgaaacgac
     3781 tgaccgaaat ggccattgaa gccatgaaga aggggggaga ccctgaggtt gccattgagg
     3841 tggtcgccaa tgctgcagaa tctgcgttga aagcggtggc tgctgccgga ccacctccag
     3901 aggttatgga gagaatgatc aaagcggcta atgaagtgat gcaacgcatg gtgcaggcag
     3961 caatcgaggc tggagctgat ggtaaagtgc ttaaaaaagc gataaaaaaa gcggcagagg
     4021 tcagcgtgaa aggaatccgg ctcgccgtcg aaaactctcc gggttaagaa ttcctagcat
     4081 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gcccctgaga cgcgtcaatc
     4141 gagttcgtac ctaagggcga caccccataa ttagcccggg cgaaaggccc agtctttcga
     4201 ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcatgg ggagtcccca
     4261 cactaccatc ggcgctacgg cgtttcactt ctgagttcgg catggggtca ggtgggacca
     4321 ccgcgctact gccgccaggc aaacaagggg tgttatgagc catattcagg tataaatggg
     4381 ctcgcgataa tgttcagaat tggttaattg gttgtaacac tgacccctat ttgtttattt
     4441 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     4501 taatattgaa aaaggaagaa tatgagtatt caacatttcc gtgtcgccct tattcccttt
     4561 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     4621 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     4681 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     4741 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     4801 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     4861 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     4921 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     4981 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     5041 gacgagcgtg acaccacgat gcctgtagcg atggcaacaa cgttgcgcaa actattaact
     5101 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     5161 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatcc
     5221 ggagccggtg agcgtggttc tcgcggtatc atcgcagcgc tggggccaga tggtaagccc
     5281 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     5341 cagatcgctg agataggtgc ctcactgatt aagcattggt aagcggcgcg ccatcgaatg
     5401 gcgcaaaacc tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt
     5461 gaatatgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac
     5521 cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga
     5581 agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa
     5641 acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat
     5701 tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt
     5761 agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt
     5821 cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc
     5881 ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat
     5941 tattttctcc catgaggacg gtacgcgact gggcgtggag catctggtcg cattgggtca
     6001 ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc
     6061 tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga
     6121 ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc
     6181 cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga
     6241 gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagatag
     6301 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac
     6361 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
     6421 gccagtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc
     6481 ccgcgcgttg gccga
//
