LOCUS       Exported                8166 bp ds-DNA     circular SYN 24-JUN-2015
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA3 Myc-DNMT3A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8166)
  AUTHORS   Addgene
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jun 24, 2015 from SnapGene 2.7.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8166
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        235..614
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        863..881
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             904..933
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /note="Myc"
                     /translation="EQKLISEEDL"
     promoter        complement(3719..3737)
                     /note="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    3763..3987
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4033..4461
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4475..4804
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      4655..4790
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     CDS             4871..5665
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    5839..5960
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6009..6025)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6033..6049
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6057..6087)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    6102..6123
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6411..6999)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7170..8030)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8031..8135)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgccacc
      901 atggagcaga agctgatctc agaggaggac ctggaattca tgcccgccat gccctccagc
      961 ggccccgggg acaccagcag ctctgctgcg gagcgggagg aggaccgaaa ggacggagag
     1021 gagcaggagg agccgcgtgg caaggaggag cgccaagagc ccagcaccac ggcacggaag
     1081 gtggggcggc ctgggaggaa gcgcaagcac cccccggtgg aaagcggtga cacgccaaag
     1141 gaccctgcgg tgatctccaa gtccccatcc atggcccagg actcaggcgc ctcagagcta
     1201 ttacccaatg gggacttgga gaagcggagt gagccccagc cagaggaggg gagccctgct
     1261 ggggggcaga agggcggggc cccagcagag ggagagggtg cagctgagac cctgcctgaa
     1321 gcctcaagag cagtggaaaa tggctgctgc acccccaagg agggccgagg agcccctgca
     1381 gaagcgggca aagaacagaa ggagaccaac atcgaatcca tgaaaatgga gggctcccgg
     1441 ggccggctgc ggggtggctt gggctgggag tccagcctcc gtcagcggcc catgccgagg
     1501 ctcaccttcc aggcggggga cccctactac atcagcaagc gcaagcggga cgagtggctg
     1561 gcacgctgga aaagggaggc tgagaagaaa gccaaggtca ttgcaggaat gaatgctgtg
     1621 gaagaaaacc aggggcccgg ggagtctcag aaggtggagg aggccagccc tcctgctgtg
     1681 cagcagccca ctgaccccgc atcccccact gtggctacca cgcctgagcc cgtggggtcc
     1741 gatgctgggg acaagaatgc caccaaagca ggcgatgacg agccagagta cgaggacggc
     1801 cggggctttg gcattgggga gctggtgtgg gggaaactgc ggggcttctc ctggtggcca
     1861 ggccgcattg tgtcttggtg gatgacgggc cggagccgag cagctgaagg cacccgctgg
     1921 gtcatgtggt tcggagacgg caaattctca gtggtgtgtg ttgagaagct gatgccgctg
     1981 agctcgtttt gcagtgcgtt ccaccaggcc acgtacaaca agcagcccat gtaccgcaaa
     2041 gccatctacg aggtcctgca ggtggccagc agccgcgcgg ggaagctgtt cccggtgtgc
     2101 cacgacagcg atgagagtga cactgccaag gccgtggagg tgcagaacaa gcccatgatt
     2161 gaatgggccc tggggggctt ccagccttct ggccctaagg gcctggagcc accagaagaa
     2221 gagaagaatc cctacaaaga agtgtacacg gacatgtggg tggaacctga ggcagctgcc
     2281 tacgcaccac ctccaccagc caaaaagccc cggaagagca cagcggagaa gcccaaggtc
     2341 aaggagatta ttgatgagcg cacaagagag cggctggtgt acgaggtgcg gcagaagtgc
     2401 cggaacattg aggacatctg catctcctgt gggagcctca atgttaccct ggaacacccc
     2461 ctcttcgttg gaggaatgtg ccaaaactgc aagaactgct ttctggagtg tgcgtaccag
     2521 tacgacgacg acggctacca gtcctactgc accatctgct gtgggggccg tgaggtgctc
     2581 atgtgcggaa acaacaactg ctgcaggtgc ttttgcgtgg agtgtgtgga cctcttggtg
     2641 gggccggggg ctgcccaggc agccattaag gaagacccct ggaactgcta catgtgcggg
     2701 cacaagggta cctacgggct gctgcggcgg cgagaggact ggccctcccg gctccagatg
     2761 ttcttcgcta ataaccacga ccaggaattt gaccctccaa aggtttaccc acctgtccca
     2821 gctgagaaga ggaagcccat ccgggtgctg tctctctttg atggaatcgc tacagggctc
     2881 ctggtgctga aggacttggg cattcaggtg gaccgctaca ttgcctcgga ggtgtgtgag
     2941 gactccatca cggtgggcat ggtgcggcac caggggaaga tcatgtacgt cggggacgtc
     3001 cgcagcgtca cacagaagca tatccaggag tggggcccat tcgatctggt gattgggggc
     3061 agtccctgca atgacctctc catcgtcaac cctgctcgca agggcctcta cgagggcact
     3121 ggccggctct tctttgagtt ctaccgcctc ctgcatgatg cgcggcccaa ggagggagat
     3181 gatcgcccct tcttctggct ctttgagaat gtggtggcca tgggcgttag tgacaagagg
     3241 gacatctcgc gatttctcga gtccaaccct gtgatgattg atgccaaaga agtgtcagct
     3301 gcacacaggg cccgctactt ctggggtaac cttcccggta tgaacaggcc gttggcatcc
     3361 actgtgaatg ataagctgga gctgcaggag tgtctggagc atggcaggat agccaagttc
     3421 agcaaagtga ggaccattac tacgaggtca aactccataa agcagggcaa agaccagcat
     3481 tttcctgtct tcatgaatga gaaagaggac atcttatggt gcactgaaat ggaaagggta
     3541 tttggtttcc cagtccacta tactgacgtc tccaacatga gccgcttggc gaggcagaga
     3601 ctgctgggcc ggtcatggag cgtgccagtc atccgccacc tcttcgctcc gctgaaggag
     3661 tattttgcgt gtgtgtaagg atccgcggcc gctcgagcat gcatctagag ggccctattc
     3721 tatagtgtca cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc
     3781 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     3841 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     3901 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     3961 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     4021 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     4081 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     4141 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     4201 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     4261 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     4321 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     4381 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     4441 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     4501 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     4561 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     4621 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     4681 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     4741 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     4801 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg
     4861 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     4921 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     4981 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     5041 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     5101 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     5161 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     5221 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     5281 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     5341 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     5401 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     5461 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     5521 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     5581 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     5641 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     5701 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     5761 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     5821 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     5881 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     5941 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     6001 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     6061 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     6121 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     6181 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     6241 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6301 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6361 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6421 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6481 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6541 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     6601 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     6661 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     6721 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     6781 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     6841 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     6901 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     6961 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7021 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7081 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7141 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7201 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7261 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7321 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7381 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7441 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     7501 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     7561 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     7621 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     7681 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     7741 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     7801 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     7861 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     7921 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     7981 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8041 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8101 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8161 gacgtc
//
