LOCUS Exported 10297 bp ds-DNA circular SYN 24-JUN-2015 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pcDNA3 Myc-DNMT1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10297) AUTHORS Addgene TITLE Direct Submission JOURNAL Exported Wednesday, Jun 24, 2015 from SnapGene 2.7.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10297 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 235..614 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" promoter 863..881 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" CDS 904..933 /codon_start=1 /product="Myc (human c-Myc oncogene) epitope tag" /note="Myc" /translation="EQKLISEEDL" promoter complement(5850..5868) /note="SP6 promoter" /note="promoter for bacteriophage SP6 RNA polymerase" polyA_signal 5894..6118 /note="bGH poly(A) signal" /note="bovine growth hormone polyadenylation signal" rep_origin 6164..6592 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 6606..6935 /note="SV40 promoter" /note="SV40 enhancer and early promoter" rep_origin 6786..6921 /note="SV40 ori" /note="SV40 origin of replication" CDS 7002..7796 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 7970..8091 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" primer_bind complement(8140..8156) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" protein_bind 8164..8180 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8188..8218) /note="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 8233..8254 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(8542..9130) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(9301..10161) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(10162..10266) /gene="bla" /note="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgccacc 901 atggagcaga agctgatctc agaggaggac ctggaattca tgccggcgcg taccgcccca 961 gcccgggtgc ccacactggc cgtcccggcc atctcgctgc ccgacgatgt ccgcaggcgg 1021 ctcaaagatt tggaaagaga cagcttaaca gaaaaggaat gtgtgaagga gaaattgaat 1081 ctcttgcacg aatttctgca aacagaaata aagaatcagt tatgtgactt ggaaaccaaa 1141 ttacgtaaag aagaattatc cgaggagggc tacctggcta aagtcaaatc ccttttaaat 1201 aaagatttgt ccttggagaa cggtgctcat gcttacaacc gggaagtgaa tggacgtcta 1261 gaaaacggga accaagcaag aagtgaagcc cgtagagtgg gaatggcaga tgccaacagc 1321 ccccccaaac ccctttccaa acctcgcacg cccaggagga gcaagtccga tggagaggct 1381 aagcctgaac cttcacctag ccccaggatt acaaggaaaa gcaccaggca aaccaccatc 1441 acatctcatt ttgcaaaggg ccctgccaaa cggaaacctc aggaagagtc tgaaagagcc 1501 aaatcggatg agtccatcaa ggaagaagac aaagaccagg atgagaagag acgtagagtt 1561 acatccagag aacgagttgc tagaccgctt cctgcagaag aacctgaaag agcaaaatca 1621 ggaacgcgca ctgaaaagga agaagaaaga gatgaaaaag aagaaaagag actccgaagt 1681 caaaccaaag aaccaacacc caaacagaaa ctgaaggagg agccggacag agaagccagg 1741 gcaggcgtgc aggctgacga ggacgaagat ggagacgaga aagatgagaa gaagcacaga 1801 agtcaaccca aagatctagc tgccaaacgg aggcccgaag aaaaagaacc tgaaaaagta 1861 aatccacaga tttctgatga aaaagacgag gatgaaaagg aggagaagag acgcaaaacg 1921 acccccaaag aaccaacgga gaaaaaaatg gctcgcgcca aaacagtcat gaactccaag 1981 acccaccctc ccaagtgcat tcagtgcggg cagtacctgg acgaccctga cctcaaatat 2041 gggcagcacc caccagacgc ggtggatgag ccacagatgc tgacaaatga gaagctgtcc 2101 atctttgatg ccaacgagtc tggctttgag agttatgagg cgcttcccca gcacaaactg 2161 acctgcttca gtgtgtactg taagcacggt cacctgtgtc ccatcgacac cggcctcatc 2221 gagaagaata tcgaactctt cttttctggt tcagcaaaac caatctatga tgatgaccca 2281 tctcttgaag gtggtgttaa tggcaaaaat cttggcccca taaatgaatg gtggatcact 2341 ggctttgatg gaggtgaaaa ggccctcatc ggcttcagca cctcatttgc cgaatacatt 2401 ctgatggatc ccagtcccga gtatgcgccc atatttgggc tgatgcagga gaagatctac 2461 atcagcaaga ttgtggtgga gttcctgcag agcaattccg actcgaccta tgaggacctg 2521 atcaacaaga tcgagaccac ggttcctcct tctggcctca acttgaaccg cttcacagag 2581 gactccctcc tgcgacacgc gcagtttgtg gtggagcagg tggagagtta tgacgaggcc 2641 ggggacagtg atgagcagcc catcttcctg acgccctgca tgcgggacct gatcaagctg 2701 gctggggtca cgctgggaca gaggcgagcc caggcgaggc ggcagaccat caggcattct 2761 accagggaga aggacagggg acccacgaaa gccaccacca ccaagctggt ctaccagatc 2821 ttcgatactt tcttcgcaga gcaaattgaa aaggatgaca gagaagacaa ggagaacgcc 2881 tttaagcgcc ggcgatgtgg cgtctgtgag gtgtgtcagc agcctgagtg tgggaaatgt 2941 aaagcctgca aggacatggt taaatttggt ggcagtggac ggagcaagca ggcttgccaa 3001 gagcggaggt gtcccaatat ggccatgaag gaggcagatg acgatgagga agtcgatgat 3061 aacatcccag agatgccgtc acccaaaaaa atgcaccagg ggaagaagaa gaaacagaac 3121 aagaatcgca tctcttgggt cggagaagcc gtcaagactg atgggaagaa gagttactat 3181 aagaaggtgt gcattgatgc ggaaaccctg gaagtggggg actgtgtctc tgttattcca 3241 gatgattcct caaaaccgct gtatctagca agggtcacgg cgctgtggga ggacagcagc 3301 aacgggcaga tgtttcacgc ccactggttc tgcgctggga cagacacagt cctcggggcc 3361 acgtcggacc ctctggagct gttcttggtg gatgaatgtg aggacatgca gctttcatat 3421 atccacagca aagtgaaagt catctacaaa gccccctccg aaaactgggc catggaggga 3481 ggcatggatc ccgagtccct gctggagggg gacgacggga agacctactt ctaccagctg 3541 tggtatgatc aagactacgc gagattcgag tcccctccaa aaacccagcc aacagaggac 3601 aacaagttca aattctgtgt gagctgtgcc cgtctggctg agatgaggca aaaagaaatc 3661 cccagggtcc tggagcagct cgaggacctg gatagccggg tcctctacta ctcagccacc 3721 aagaacggca tcctgtaccg agttggtgat ggtgtgtacc tgccccctga ggccttcacg 3781 ttcaacatca agctgtccag tcccgtgaaa cgcccacgga aggagcccgt ggatgaggac 3841 ctgtacccag agcactaccg gaaatactcc gactacatca aaggcagcaa cctggatgcc 3901 cctgagccct accgaattgg ccggatcaaa gagatcttct gtcccaagaa gagcaacggc 3961 aggcccaatg agactgacat caaaatccgg gtcaacaagt tctacaggcc tgagaacacc 4021 cacaagtcca ctccagcgag ctaccacgca gacatcaacc tgctctactg gagcgacgag 4081 gaggccgtgg tggacttcaa ggctgtgcag ggccgctgca ccgtggagta tggggaggac 4141 ctgcccgagt gcgtccaggt gtactccatg ggcggcccca accgcttcta cttcctcgag 4201 gcctataatg caaagagcaa aagctttgaa gatcctccca accatgcccg tagccctgga 4261 aacaaaggga agggcaaggg aaaagggaag ggcaagccca agtcccaagc ctgtgagccg 4321 agcgagccag agatagagat caagctgccc aagctgcgga ccctggatgt gttttctggc 4381 tgcggggggt tgtcggaggg attccaccaa gcaggcatct ctgacacgct gtgggccatc 4441 gagatgtggg accctgcggc ccaggcgttc cggctgaaca accccggctc cacagtgttc 4501 acagaggact gcaacatcct gctgaagctg gtcatggctg gggagaccac caactcccgc 4561 ggccagcggc tgccccagaa gggagacgtg gagatgctgt gcggcgggcc gccctgccag 4621 ggcttcagcg gcatgaaccg cttcaattcg cgcacctact ccaagttcaa aaactctctg 4681 gtggtttcct tcctcagcta ctgcgactac taccggcccc ggttcttcct cctggagaat 4741 gtcaggaact ttgtctcctt caagcgctcc atggtcctga agctcaccct ccgctgcctg 4801 gtccgcatgg gctatcagtg caccttcggc gtgctgcagg ccggtcagta cggcgtggcc 4861 cagactagga ggcgggccat catcctggcc gcggcccctg gagagaagct ccctctgttc 4921 ccggagccac tgcacgtgtt tgctccccgg gcctgccagc tgagcgtggt ggtggatgac 4981 aagaagtttg tgagcaacat aaccaggttg agctcgggtc ctttccggac catcacggtg 5041 cgagacacga tgtccgacct gccggaggtg cggaatggag cctcggcact ggagatctcc 5101 tacaacgggg agcctcagtc ctggttccag aggcagctcc ggggcgcaca gtaccagccc 5161 atcctcaggg accacatctg taaggacatg agtgcattgg tggctgcccg catgcggcac 5221 atccccttgg ccccagggtc agactggcgc gatctgccca acatcgaggt gcggctctca 5281 gacggcacca tggccaggaa gctgcggtat acccaccatg acaggaagaa cggccgcagc 5341 agctctgggg ccctccgtgg ggtctgctcc tgcgtggaag ccggcaaagc ctgcgacccc 5401 gcagccaggc agttcaacac cctcatcccc tggtgcctgc cccacaccgg gaaccggcac 5461 aaccactggg ctggcctcta tggaaggctc gagtgggacg gcttcttcag cacaaccgtc 5521 accaaccccg agcccatggg caagcagggc cgcgtgctcc acccagagca gcaccgtgtg 5581 gtgagcgtgc gggagtgtgc ccgctcccag ggcttccctg acacctaccg gctcttcggc 5641 aacatcctgg acaagcaccg gcaggtgggc aatgccgtgc caccgcccct ggccaaagcc 5701 attggcttgg agatcaagct ttgtatgttg gccaaagccc gagagagtgc ctcagctaaa 5761 ataaaggagg aggaagctgc taaggactag gtcgaccgag atctctcgag gtaccgcggc 5821 cgctcgagca tgcatctaga gggccctatt ctatagtgtc acctaaatgc tagagctcgc 5881 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg 5941 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt 6001 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc 6061 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct 6121 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc 6181 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc 6241 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc 6301 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc 6361 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg 6421 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact 6481 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt 6541 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt 6601 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 6661 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag 6721 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc 6781 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 6841 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt 6901 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca 6961 ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat 7021 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac 7081 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc 7141 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc 7201 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag 7261 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc 7321 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg 7381 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc 7441 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc 7501 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga 7561 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca 7621 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg 7681 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg 7741 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg 7801 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga 7861 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg 7921 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat 7981 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 8041 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 8101 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg 8161 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 8221 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 8281 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 8341 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 8401 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 8461 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 8521 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 8581 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 8641 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 8701 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 8761 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 8821 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 8881 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 8941 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg 9001 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 9061 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 9121 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 9181 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 9241 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 9301 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 9361 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 9421 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 9481 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 9541 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 9601 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 9661 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 9721 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 9781 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 9841 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 9901 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 9961 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 10021 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 10081 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 10141 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 10201 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 10261 tccgcgcaca tttccccgaa aagtgccacc tgacgtc //