LOCUS       Cre Stoplight 2.        5241 bp    DNA     circular SYN 18-Jun-2014
DEFINITION  Cre Stoplight 2.4
ACCESSION   Cre Stoplight 2.4
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     Textco/File created by Gene Construction Kit (Textco BioSoftware)
FEATURES             Location/Qualifiers
     misc_feature    4632..4633
     misc_feature    complement(3634..4595)
                     /label="ampicillin resistance gene"
     misc_feature    complement(2821..3491)
                     /label="pUC origin"
     misc_feature    2308..2438
                     /label="SV40 poly A"
     misc_feature    195..199
                     /label="tata box"
     misc_feature    complement(4767..4785)
                     /label="good lower primer for mut"
                     /note="I designed this primer to amplify the entire primer minus the
                      promoter region"
     misc_feature    4864..5241
                     /label="CMV promoter"
     misc_feature    1418..1451
                     /label="loxP"
     misc_feature    1269..1397
                     /label="T7 transcription termination"
     misc_feature    254..273
                     /label="T7 primer"
     misc_feature    292..325
                     /label="loxH"
     misc_feature    221..227
                     /label="putative transcription start site"
     misc_feature    248..267
                     /label="Good upper for mutagenesis"
     misc_feature    1047..1255
                     /label="BGH poly A"
     CDS             1471..2190
                     /label="mCherry"
     CDS             339..1034
                     /label="ZsGreen1"
ORIGIN
        1 CCATGGTGAT GCGGTTTTGG CAGTACATCA ATGGGCGTGG ATAGCGGTTT GACTCACGGG  
       61 GATTTCCAAG TCTCCACCCC ATTGACGTCA ATGGGAGTTT GTTTTGGCAC CAAAATCAAC  
      121 GGGACTTTCC AAAATGTCGT AACAACTCCG CCCCATTGAC GCAAATGGGC GGTAGGCGTG  
      181 TACGGTGGGA GGTCTATATA AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT  
      241 GGCTTATCGA AATTAATACG ACTCACTATA GGGAGACCCA AGCTGGCTAG AATTACCTCA  
      301 TATAGCATAC ATTATACGAA GTTATGGGCG CGCTCGAGAT GGCCCAGTCC AAGCACGGCC  
      361 TGACCAAGGA GATGACCATG AAGTACCGCA TGGAGGGCTG CGTGGACGGC CACAAGTTCG  
      421 TGATCACCGG CGAGGGCATC GGCTACCCCT TCAAGGGCAA GCAGGCCATC AACCTGTGCG  
      481 TGGTGGAGGG CGGCCCCTTG CCCTTCGCCG AGGACATCTT GTCCGCCGCC TTCATGTACG  
      541 GCAACCGCGT GTTCACCGAG TACCCCCAGG ACATCGTCGA CTACTTCAAG AACTCCTGCC  
      601 CCGCCGGCTA CACCTGGGAC CGCTCCTTCC TGTTCGAGGA CGGCGCCGTG TGCATCTGCA  
      661 ACGCCGACAT CACCGTGAGC GTGGAGGAGA ACTGCATGTA CCACGAGTCC AAGTTCTACG  
      721 GCGTGAACTT CCCCGCCGAC GGCCCCGTGA TGAAGAAGAT GACCGACAAC TGGGAGCCCT  
      781 CCTGCGAGAA GATCATCCCC GTGCCCAAGC AGGGCATCTT GAAGGGCGAC GTGAGCATGT  
      841 ACCTGCTGCT GAAGGACGGT GGCCGCTTGC GCTGCCAGTT CGACACCGTG TACAAGGCCA  
      901 AGTCCGTGCC CCGCAAGATG CCCGACTGGC ACTTCATCCA GCACAAGCTG ACCCGCGAGG  
      961 ACCGCAGCGA CGCCAAGAAC CAGAAGTGGC ACCTGACCGA GCACGCCATC GCCTCCGGCT  
     1021 CCGCCTTGCC CTGACTCGAG CGCGCCCTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG  
     1081 CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA  
     1141 AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT  
     1201 GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATCTAGA  
     1261 AGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA  
     1321 ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG  
     1381 AGGAACTATA TCCGGATATC CCAGCGGCCG CGGTACCATA ACTTCGTATA GCATACATTA  
     1441 TACGAAGTTA TGTTTcggAA TTCGCGCGCA ATGGTGAGCA AGGGCGAGGA GGATAACATG  
     1501 GCCATCATCA AGGAGTTCAT GCGCTTCAAG GTGCACATGG AGGGCTCCGT GAACGGCCAC  
     1561 GAGTTCGAGA TCGAGGGCGA GGGCGAGGGC CGCCCCTACG AGGGCACCCA GACCGCCAAG  
     1621 CTGAAGGTGA CCAAGGGTGG CCCCCTGCCC TTCGCCTGGG ACATCCTGTC CCCTCAGTTC  
     1681 ATGTACGGCT CCAAGGCCTA CGTGAAGCAC CCCGCCGACA TCCCCGACTA CTTGAAGCTG  
     1741 TCCTTCCCCG AGGGCTTCAA GTGGGAGCGC GTGATGAACT TCGAGGACGG CGGCGTGGTG  
     1801 ACCGTGACCC AGGACTCCTC CCTGCAGGAC GGCGAGTTCA TCTACAAGGT GAAGCTGCGC  
     1861 GGCACCAACT TCCCCTCCGA CGGCCCCGTA ATGCAGAAGA AGACCATGGG CTGGGAGGCC  
     1921 TCCTCCGAGC GGATGTACCC CGAGGACGGC GCCCTGAAGG GCGAGATCAA GCAGAGGCTG  
     1981 AAGCTGAAGG ACGGCGGCCA CTACGACGCT GAGGTCAAGA CCACCTACAA GGCCAAGAAG  
     2041 CCCGTGCAGC TGCCCGGCGC CTACAACGTC AACATCAAGT TGGACATCAC CTCCCACAAC  
     2101 GAGGACTACA CCATCGTGGA ACAGTACGAA CGCGCCGAGG GCCGCCACTC CACCGGCGGC  
     2161 ATGGACGAGC TGTACAAGGG GCGCGCCTAG GaaTTCGATT CCACCGCCGC CTTCTATGAA  
     2221 AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT  
     2281 CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA  
     2341 TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT  
     2401 GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG  
     2461 AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT  
     2521 CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC  
     2581 TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC  
     2641 CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT  
     2701 TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA  
     2761 GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC  
     2821 ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT  
     2881 TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG  
     2941 CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC  
     3001 TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC  
     3061 GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC  
     3121 AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC  
     3181 TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT  
     3241 AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT  
     3301 AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC  
     3361 TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTTTT  
     3421 TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC  
     3481 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG  
     3541 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA  
     3601 ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA  
     3661 CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG  
     3721 ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC  
     3781 CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC  
     3841 AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT  
     3901 AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC  
     3961 GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG  
     4021 CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC  
     4081 GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT  
     4141 TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG  
     4201 TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT  
     4261 AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG  
     4321 CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA  
     4381 CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA  
     4441 AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC  
     4501 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA  
     4561 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG  
     4621 CCACCTGACG TCGACGGATC GGGAGATCTC CCGATCCCCT ATGGTGCACT CTCAGTACAA  
     4681 TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATCTGCTCCC TGCTTGTGTG TTGGAGGTCG  
     4741 CTGAGTAGTG CGCGAGCAAA ATTTAAGCTA CAACAAGGCA AGGCTTGACC GACAATTGCA  
     4801 TGAAGAATCT GCTTAGGGTT AGGCGTTTTG CGCTGCTTCG CGATGTACGG GCCAGATATA  
     4861 CGCGTTGACA TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC  
     4921 ATAGCCCATA TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC  
     4981 CGCCCAACGA CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA  
     5041 TAGGGACTTT CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG  
     5101 TACATCAAGT GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC  
     5161 CCGCCTGGCA TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT  
     5221 ACGTATTAGT CATCGCTATT A 
//
