LOCUS       pAAV-CAG-GFP            5442 bp    DNA     circular     11-MAY-2012
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|625832611|
COMMENT     VNTDBDATE|625832611|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV-CAG-GFP|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|Mingjie Li|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "Currently local object. Original author: Mingjie Li" 
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FEATURES             Location/Qualifiers
     misc_feature    2651..3587
                     /vntifkey="21"
                     /label=CAG\(CBA)
     misc_feature    complement(167..786)
                     /vntifkey="21"
                     /label=pBR322_origin
     misc_feature    complement(941..1801)
                     /vntifkey="21"
                     /label=Ampicilin
     misc_feature    complement(1843..1871)
                     /vntifkey="21"
                     /label=Ampr\promoter
     misc_feature    complement(4972..5091)
                     /vntifkey="21"
                     /label=SV40_PA_terminator
     misc_feature    4346..4933
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    2458..2647
                     /vntifkey="21"
                     /label=ITR
     misc_feature    complement(5199..5388)
                     /vntifkey="21"
                     /label=ITR
     exon            3600..4319
                     /vntifkey="61"
                     /label=GFP
BASE COUNT     1219 a      1486 c      1460 g      1277 t 
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta 
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 
     1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta 
     1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga 
     2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa 
     2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga 
     2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc 
     2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga 
     2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg 
     2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt 
     2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct 
     2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg 
     2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag 
     2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag 
     2641 gaagatcgta ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac 
     2701 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca 
     2761 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg 
     2821 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt 
     2881 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc 
     2941 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat 
     3001 gggggcgggg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg 
     3061 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc 
     3121 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg 
     3181 agtcgctgcg acgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc 
     3241 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc 
     3301 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa 
     3361 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ctgtccgcgg 
     3421 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc 
     3481 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg 
     3541 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattgga tccgccacca 
     3601 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg 
     3661 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg 
     3721 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc 
     3781 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc 
     3841 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct 
     3901 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg 
     3961 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca 
     4021 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg 
     4081 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg 
     4141 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact 
     4201 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc 
     4261 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag 
     4321 aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga 
     4381 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 
     4441 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 
     4501 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 
     4561 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 
     4621 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 
     4681 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat 
     4741 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 
     4801 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 
     4861 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 
     4921 ccgcctcccc gcatcgatac cgtcgacccg ggcggccgct tcgagcagac atgataagat 
     4981 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg 
     5041 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca 
     5101 acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag gttttttaaa 
     5161 gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tcttcctaga gcatggctac 
     5221 gtagataagt agcatggcgg gttaatcatt aactacaagg aacccctagt gatggagttg 
     5281 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga 
     5341 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cattaatgaa 
     5401 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ct 
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