LOCUS       pQFlag-USP11       10080 bp    DNA     circular     30-SEP-2011
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|436296834|
COMMENT     VNTDBDATE|602599824|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pQFlag-USP11|
COMMENT     VNTAUTHORNAME|hghg|
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FEATURES             Location/Qualifiers
     promoter        1..511
                     /vntifkey="29"
                     /label=CMV/MSV
     misc_feature    584..654
                     /vntifkey="21"
                     /label=R
     misc_feature    655..728
                     /vntifkey="21"
                     /label=U5
     misc_feature    758..1567
                     /vntifkey="21"
                     /label=Psi
     promoter        1601..2132
                     /vntifkey="29"
                     /label=CMV\promotor
     RBS             5207..5780
                     /vntifkey="32"
                     /label=IRES
     CDS             5813..6412
                     /vntifkey="4"
                     /label=Puro\R
     LTR             6786..7211
                     /vntifkey="19"
                     /label=3'\MoMuLV\LTR\(deletion\in\U3)
     promoter        7491..7758
                     /vntifkey="29"
                     /label=SV40\prom
     rep_origin      7712..7777
                     /vntifkey="33"
                     /label=SV40\ori
     CDS             complement(8858..9718)
                     /vntifkey="4"
                     /label=AmpR
     CDS             2265..2288
                     /vntifkey="4"
                     /label=Flag
     CDS             2295..5186
                     /vntifkey="4"
                     /label=USP11
     CDS             2265..2288
                     /vntifkey="4"
                     /label=Flag
BASE COUNT     2310 a      2840 c      2679 g      2251 t 
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc 
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac 
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc 
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc 
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc 
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc 
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat 
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc 
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc 
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta 
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc 
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc 
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca 
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct 
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc 
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga 
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt 
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg 
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga 
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa 
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct 
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct 
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg 
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac 
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt 
     1921 ggcagtacat caatgggcgt gaatagcggt ttgactcacg gggatttcca agtctccacc 
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc 
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata 
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg 
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa 
     2221 cgctgcagga attgatccgc ggccgcgaca ccatggcaca attggattac aaggatgacg 
     2281 atgacaagac cggtatggca gtagccccgc gactgtttgg ggggctctgc ttccgtttcc 
     2341 gggaccagaa tccggaagtg gctgttgagg ggcgtcttcc aatctcgcac agctgcgttg 
     2401 gctgtagaag agaacggacg gcgatggcga cggtcgcagc aaatccagct gctgctgcgg 
     2461 cggctgtggc ggcggcagcg gcggtgactg aggatagaga gccacagcac gaggagctgc 
     2521 caggcctgga cagccagtgg cgccagatag aaaacggcga gagtgggcga gaacgtccac 
     2581 tgcgggccgg cgaaagctgg ttccttgtgg agaagcactg gtataagcag tgggaggcat 
     2641 acgtgcaggg aggggaccag gactccagca ccttccctgg ctgcatcaac aatgccacac 
     2701 tctttcaaga tgagataaac tggcgcctca aggagggact ggtggaaggc gaggattatg 
     2761 tgctgctccc agcagctgct tggcattacc tggtcagctg gtatggtcta gagcatggcc 
     2821 agccacccat tgaacgcaag gtcatagagc tgcccaacat ccagaaggtc gaagtgtacc 
     2881 cagtagaact gctgcttgtc cggcacaatg atttgggcaa atctcacact gttcagttca 
     2941 gccataccga ttctattggc ctagtattgc gcacagctcg ggagcggttt ctggtggagc 
     3001 cccaggaaga cactcggctt tgggccaaga actcagaagg ctctttggat aggttgtatg 
     3061 acacacacat cacggttctc gatgcggccc ttgagactgg gcagttgatc atcatggaga 
     3121 cccgcaagaa agatggcact tggcccagcg cacagctgca tgtcatgaac aacaacatgt 
     3181 cggaagagga tgaggacttc aagggtcagc caggcatctg tggcctcacc aatctgggca 
     3241 acacgtgctt catgaactcg gccctgcagt gcctcagcaa tgtgccacag ctcaccgagt 
     3301 acttcctcaa caactgctac ctggaggagc tcaacttccg caacccactg ggcatgaagg 
     3361 gtgagatcgc agaggcctat gcagacctgg tgaagcaggc gtggtctggc caccaccgct 
     3421 ccattgtgcc acatgtgttc aagaacaagg ttggccattt tgcatcccaa tttctgggct 
     3481 accagcagca tgactctcag gagctgctgt cattcctcct ggacgggctg catgaggacc 
     3541 ttaatcgggt gaagaagaag gagtatgtgg agctgtgcga tgctgctggg cgaccggatc 
     3601 aggaggtggc acaggaggca tggcaaaacc acaaacggcg gaacgattct gtgatcgtgg 
     3661 acactttcca cggcctcttc aagtccacgc tggtgtgccc cgattgtggc aatgtatctg 
     3721 tgaccttcga ccccttctgc tacctcagtg ttccactgcc tatcagccac aagagggtct 
     3781 tggaggtctt ctttatcccc atggatccgc gccgcaagcc agagcagcac cggctcgtgg 
     3841 tccccaagaa aggcaagatc tcggatctat gtgtggctct gtccaaacac acgggcatct 
     3901 cgccagagag gatgatggtg gctgatgtct tcagtcaccg cttctataag ctctatcagc 
     3961 tagaggagcc tctgagcagc atcttggacc gtgatgatat cttcgtctat gaggtgtcag 
     4021 gtcgcattga ggccattgag ggctcaagag aggacatcgt ggttcctgtc tacctgcggg 
     4081 agcgcacccc tgcccgtgac tacaacaact cctactacgg cctgatgctt tttggacacc 
     4141 ccctcctggt atcagtgccc cgggaccgct tcacctggga gggcctgtat aacgtcctga 
     4201 tgtaccggct ctcacgctac gtgaccaaac ccaactcaga tgatgaggac gatggggatg 
     4261 agaaagaaga tgacgaggag gataaagatg acgtccctgg gccctcaact gggggcagcc 
     4321 tccgagaccc tgagccagag caggctgggc ccagctctgg agtcacgaac aggtgcccgt 
     4381 tcctcctgga caattgcctt ggcacatctc agtggccccc aaggcgacga cgcaagcagc 
     4441 tgttcaccct gcagacggtg aactccaatg ggaccagcga ccgcacaacc tcccctgaag 
     4501 aagtccatgc ccagccgtac attgctatcg actgggagcc agagatgaag aagcgttact 
     4561 atgacgaggt agaggctgag ggctacgtga agcatgactg cgtcgggtac gtgatgaaga 
     4621 aggctcccgt gcggctgcag gagtgcattg agctcttcac cactgtggag accctggaga 
     4681 aggaaaaccc ctggtactgc ccttcctgca agcagcacca gctggcaacc aagaagctgg 
     4741 acctgtggat gctgccggag attctcatca tccacctgaa acgcttttcc tacaccaagt 
     4801 tctcccgaga gaagctggac accctcgtgg agtttcctat ccgggacctg gacttctctg 
     4861 agtttgtcat ccagccacag aatgagtcga atccggagct gtacaaatat gacctcatcg 
     4921 cggtttccaa ccattatggg ggcatgcgtg atggacacta cacaacattt gcctgcaaca 
     4981 aggacagcgg ccagtggcac tactttgatg acaacagcgt ctcccctgtc aatgagaatc 
     5041 agatcgagtc caaggcagcc tatgtcctct tctaccaacg ccaggacgtg gcgcgacgcc 
     5101 tgctgtcccc ggccggctca tctggcgccc cagcctcccc tgcctgcagc tccccaccca 
     5161 gctctgagtt catggatgtt aattgactcg agggatccgg aattccgccc ctctccctcc 
     5221 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat 
     5281 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct 
     5341 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg 
     5401 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta 
     5461 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag 
     5521 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg 
     5581 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat 
     5641 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca 
     5701 tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc 
     5761 tttgaaaaac acgatgataa gcttgccaca acccacaagg agacgacctt ccatgaccga 
     5821 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct 
     5881 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat 
     5941 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa 
     6001 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga 
     6061 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct 
     6121 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg 
     6181 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt 
     6241 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc 
     6301 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga 
     6361 gtgcccgaag gaccgcgcga cctggtgcat gacccgcaag cccggtgcct gacgcccgcc 
     6421 ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catggctccg accgaagccg 
     6481 acccgggcgg ccccgccgac cccgcacccg cccccgaggc ccaccgactc tagtcgaggg 
     6541 ctgcagcgct gcagaggccg agtgcagaac tgctccaaag ggacctcaag gctttccgag 
     6601 ggacactagg ctgactccat cgagccagtg tagagataag cttatcgatt agtccaattt 
     6661 gttaaagaca ggatatcagt ggtccaggct ctagttttga ctcaacaata tcaccagctg 
     6721 aagcctatag agtacgagcc atagataaaa taaaagattt tatttagtct ccagaaaaag 
     6781 gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc 
     6841 aaggcatgga aaaatacata actgagaata gagaagttca gatcaaggtc aggaacagat 
     6901 ggaacagggt cgaccctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa 
     6961 tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct 
     7021 tctgctcccc gagctcaata aaagagccca caacccctca ctcggggcgc cagtcctccg 
     7081 attgactgag tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac 
     7141 ttgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg 
     7201 ggtctttcat ttgggggctc gtccgggatc gggagacccc tgcccaggga ccaccgaccc 
     7261 accaccggga ggtaagctgg ctgcctcgcg cgtttcggtg atgacggtga aaacctctga 
     7321 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa 
     7381 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca 
     7441 cgtagcgata gcggagtgta gatccggctg tggaatgtgt gtcagttagg gtgtggaaag 
     7501 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc 
     7561 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 
     7621 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 
     7681 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 
     7741 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt 
     7801 tgcaaaaagc ttactggctt aactatgcgg catcagagca gattgtactg agagtgcacc 
     7861 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt 
     7921 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 
     7981 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 
     8041 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 
     8101 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 
     8161 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 
     8221 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 
     8281 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 
     8341 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 
     8401 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 
     8461 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 
     8521 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 
     8581 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 
     8641 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 
     8701 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 
     8761 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 
     8821 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 
     8881 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 
     8941 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 
     9001 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 
     9061 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 
     9121 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca 
     9181 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 
     9241 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 
     9301 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 
     9361 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 
     9421 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg 
     9481 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 
     9541 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 
     9601 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 
     9661 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 
     9721 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 
     9781 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 
     9841 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta 
     9901 tcacgaggcc ctttcgtctt caagaattag cttggccatt gcatacgttg tatccatatc 
     9961 ataatatgta catttatatt ggctcatgtc caacattacc gccatgttga cattgattat 
    10021 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt 
//
