LOCUS       pQFlag-USP7       10491 bp    DNA     circular     23-JAN-2007
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|436121880|
COMMENT     VNTDBDATE|436121880|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pQFlag-USP7|
COMMENT     VNTAUTHORNAME|hghg|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     promoter        1..511
                     /vntifkey="29"
                     /label=CMV/MSV
     misc_feature    584..654
                     /vntifkey="21"
                     /label=R
     misc_feature    655..728
                     /vntifkey="21"
                     /label=U5
     misc_feature    758..1567
                     /vntifkey="21"
                     /label=Psi
     promoter        1601..2132
                     /vntifkey="29"
                     /label=CMV\promotor
     RBS             5618..6191
                     /vntifkey="32"
                     /label=IRES
     CDS             6224..6823
                     /vntifkey="4"
                     /label=Puro\R
     LTR             7197..7622
                     /vntifkey="19"
                     /label=3'\MoMuLV\LTR\(deletion\in\U3)
     promoter        7902..8169
                     /vntifkey="29"
                     /label=SV40\prom
     rep_origin      8123..8188
                     /vntifkey="33"
                     /label=SV40\ori
     CDS             complement(9269..10129)
                     /vntifkey="4"
                     /label=AmpR
     CDS             2265..2288
                     /vntifkey="4"
                     /label=Flag
BASE COUNT     2705 a      2654 c      2647 g      2485 t 
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc 
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac 
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc 
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc 
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc 
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc 
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat 
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc 
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc 
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta 
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc 
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc 
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca 
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct 
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc 
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga 
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt 
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg 
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga 
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa 
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct 
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct 
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg 
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac 
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt 
     1921 ggcagtacat caatgggcgt gaatagcggt ttgactcacg gggatttcca agtctccacc 
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc 
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata 
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg 
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa 
     2221 cgctgcagga attgatccgc ggccgcgaca ccatggcaca attggattac aaggatgacg 
     2281 atgacaagac cggtatgaac caccagcagc agcagcagca gcagaaagcg ggcgagcagc 
     2341 agttgagcga gcccgaggac atggagatgg aagcgggaga tacagatgac ccaccaagaa 
     2401 ttactcagaa ccctgtgatc aatgggaatg tggccctgag tgatggacac aacaccgcgg 
     2461 aggaggacat ggaggatgac accagttggc gctccgaggc aacctttcag ttcactgtgg 
     2521 agcgcttcag cagactgagt gagtcggtcc ttagccctcc gtgttttgtg cgaaatctgc 
     2581 catggaagat tatggtgatg ccacgctttt atccagacag accacaccaa aaaagcgtag 
     2641 gattctttct ccagtgcaat gctgaatctg attccacgtc atggtcttgc catgcacaag 
     2701 cagtgctgaa gataataaat tacagagatg atgaaaagtc gttcagtcgt cgtattagtc 
     2761 atttgttctt ccataaagaa aatgattggg gattttccaa ttttatggcc tggagtgaag 
     2821 tgaccgatcc tgagaaagga tttatagatg atgacaaagt tacctttgaa gtctttgtac 
     2881 aggcggatgc tccccatgga gttgcgtggg attcaaagaa gcacacaggc tacgtcggct 
     2941 taaagaatca gggagcgact tgttacatga acagcctgct acagacgtta tttttcacga 
     3001 atcagctacg aaaggctgtg tacatgatgc caaccgaggg ggatgattcg tctaaaagcg 
     3061 tccctttagc attacaaaga gtgttctatg aattacagca tagtgataaa cctgtaggaa 
     3121 caaaaaagtt aacaaagtca tttgggtggg aaactttaga tagcttcatg caacatgatg 
     3181 ttcaggagct ttgtcgagtg ttgctcgata atgtggaaaa taagatgaaa ggcacctgtg 
     3241 tagagggcac catacccaaa ttattccgcg gcaaaatggt gtcctatatc cagtgtaaag 
     3301 aagtagacta tcggtctgat agaagagaag attattatga tatccagcta agtatcaaag 
     3361 gaaagaaaaa tatatttgaa tcatttgtgg attatgtggc agtagaacag ctcgatgggg 
     3421 acaataaata cgacgctggg gaacatggct tacaggaagc agagaaaggt gtgaaattcc 
     3481 taacattgcc accagtgtta catctacaac tgatgagatt tatgtatgac cctcagacgg 
     3541 accaaaatat caagatcaat gataggtttg aattcccaga gcagttacca cttgatgaat 
     3601 ttttgcaaaa aacagatcct aaggaccctg caaattatat tcttcatgca gtcctggttc 
     3661 atagtggaga taatcatggt ggacattatg tggtttatct aaaccccaaa ggggatggca 
     3721 aatggtgtaa atttgatgac gacgtggtgt caaggtgtac taaagaggaa gcaattgagc 
     3781 acaattatgg gggtcacgat gacgacctgt ctgttcgaca ctgcactaat gcttacatgt 
     3841 tagtctacat cagggaatca aaactgagtg aagttttaca ggcggtcacc gaccatgata 
     3901 ttcctcagca gttggtggag cgattacaag aagagaaaag gatcgaggct cagaagcgga 
     3961 aggagcggca ggaagcccat ctctatatgc aagtgcagat agtcgcagag gaccagtttt 
     4021 gtggccacca agggaatgac atgtacgatg aagaaaaagt gaaatacact gtgttcaaag 
     4081 tattgaagaa ctcctcgctt gctgagtttg ttcagagcct ctctcagacc atgggatttc 
     4141 cacaagatca aattcgattg tggcccatgc aagcaaggag taatggaaca aaacgaccag 
     4201 caatgttaga taatgaagcc gacggcaata aaacaatgat tgagctcagt gataatgaaa 
     4261 acccttggac aatattcctg gaaacagttg atcccgagct ggctgctagt ggagcgacct 
     4321 tacccaagtt tgataaagat catgatgtaa tgttattttt gaagatgtat gatcccaaaa 
     4381 cgcggagctt gaattactgt gggcatatct acacaccaat atcctgtaaa atacgtgact 
     4441 tgctcccagt tatgtgtgac agagcaggat ttattcaaga tactagcctt atcctctatg 
     4501 aggaagttaa accgaattta acagagagaa ttcaggacta tgacgtgtct cttgataaag 
     4561 cccttgatga actaatggat ggtgacatca tagtatttca gaaggatgac cctgaaaatg 
     4621 ataacagtga attacccacc gcaaaggagt atttccgaga tctctaccac cgcgttgatg 
     4681 tcattttctg tgataaaaca atccctaatg atcctggatt tgtggttacg ttatcaaata 
     4741 gaatgaatta ttttcaggtt gcaaagacag ttgcacagag gctcaacaca gatccaatgt 
     4801 tgctgcagtt tttcaagtct caaggttata gggatggccc aggtaatcct cttagacata 
     4861 attatgaagg tactttaaga gatcttctac agttcttcaa gcctagacaa cctaagaaac 
     4921 tttactatca gcagcttaag atgaaaatca cagactttga gaacaggcga agttttaaat 
     4981 gtatatggtt aaacagccaa tttagggaag aggaaataac actatatcca gacaagcatg 
     5041 ggtgtgtccg ggacctgtta gaagaatgta aaaaggccgt ggagcttggg gagaaagcat 
     5101 cagggaaact taggctgcta gaaattgtaa gctacaaaat cattggtgtt catcaagaag 
     5161 atgaactatt agaatgttta tctcctgcaa cgagccggac gtttcgaata gaggaaatcc 
     5221 ctttggacca ggtggacata gacaaagaga atgagatgct tgtcacagtg gcgcatttcc 
     5281 acaaagaggt cttcggaacg ttcggaatcc cgtttttgct gaggatacac cagggcgagc 
     5341 attttcgaga agtgatgaag cgaatccaga gcctgctgga catccaggag aaggagtttg 
     5401 agaagtttaa atttgcaatt gtaatgacgg gccgacacca gtacataaat gaagacgagt 
     5461 atgaagtaaa tttgaaagac tttgagccac agcccggtaa tatgtctcat cctcggcctt 
     5521 ggctagggct cgaccacttc aacaaagccc caaagaggag tcgctacact taccttgaaa 
     5581 aggccattaa aatccataac tgaggatccg gaattccgcc cctctccctc ccccccccct 
     5641 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt 
     5701 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg 
     5761 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc 
     5821 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt 
     5881 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta 
     5941 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg 
     6001 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag 
     6061 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag 
     6121 tcgaggttaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa 
     6181 cacgatgata agcttgccac aacccacaag gagacgacct tccatgaccg agtacaagcc 
     6241 cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc 
     6301 gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt 
     6361 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt 
     6421 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc 
     6481 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca 
     6541 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc 
     6601 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc 
     6661 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg 
     6721 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg agtgcccgaa 
     6781 ggaccgcgcg acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc 
     6841 gcagcgcccg accgaaagga gcgcacgacc ccatggctcc gaccgaagcc gacccgggcg 
     6901 gccccgccga ccccgcaccc gcccccgagg cccaccgact ctagtcgagg gctgcagcgc 
     6961 tgcagaggcc gagtgcagaa ctgctccaaa gggacctcaa ggctttccga gggacactag 
     7021 gctgactcca tcgagccagt gtagagataa gcttatcgat tagtccaatt tgttaaagac 
     7081 aggatatcag tggtccaggc tctagttttg actcaacaat atcaccagct gaagcctata 
     7141 gagtacgagc catagataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg 
     7201 aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg 
     7261 aaaaatacat aactgagaat agagaagttc agatcaaggt caggaacaga tggaacaggg 
     7321 tcgaccctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt 
     7381 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc 
     7441 cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga 
     7501 gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct 
     7561 cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca 
     7621 tttgggggct cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg 
     7681 aggtaagctg gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag 
     7741 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag 
     7801 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat 
     7861 agcggagtgt agatccggct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc 
     7921 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga 
     7981 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 
     8041 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat 
     8101 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc 
     8161 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag 
     8221 cttactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt 
     8281 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct 
     8341 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 
     8401 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 
     8461 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 
     8521 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 
     8581 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 
     8641 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 
     8701 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 
     8761 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 
     8821 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 
     8881 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 
     8941 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 
     9001 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 
     9061 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 
     9121 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 
     9181 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 
     9241 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 
     9301 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 
     9361 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 
     9421 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 
     9481 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 
     9541 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt 
     9601 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 
     9661 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 
     9721 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 
     9781 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 
     9841 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg 
     9901 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 
     9961 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 
    10021 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 
    10081 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 
    10141 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 
    10201 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 
    10261 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 
    10321 cctttcgtct tcaagaatta gcttggccat tgcatacgtt gtatccatat cataatatgt 
    10381 acatttatat tggctcatgt ccaacattac cgccatgttg acattgatta ttgactagtt 
    10441 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag t 
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