LOCUS       pEMS2023\(hs1218)        7035 bp    DNA     circular      6-JUL-2016
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11313554|
COMMENT     VNTDBDATE|-11313554|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEMS2023 (hs1218)|
COMMENT     VNTAUTHORNAME|Siu Ling Lam|
COMMENT     VNTAUTHORTEL|6048753877|
COMMENT     VNTAUTHOREML|sling@cmmt.ubc.ca|
COMMENT     VNTOAUTHORNAME|User|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #2=(CRSite (CStringList) "AvrII" "cctagg" 2 0 1 198 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 6 32 0 2558 0 3149 0 
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COMMENT        #5=(CRSite (CStringList) "BglII" "agatct" 2 0 2 1669 0 3053 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 192 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "FseI" "ggccggcc" 7 0 1 207 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "MluI" "acgcgt" 2 0 1 210 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 2 2742 0 3786 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "SmaI" "cccggg" 4 0 5 32 0 43 0 3527 0 4184 0 
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COMMENT        #11=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 1 3795 0 0 "") 
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COMMENT             (CStringList) 1 1 1 1 "6612..7200") 
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COMMENT        #25=(CFSignal (CObList) "MCS 5' end" 21 0 0 197 208 0 (CStringList) 
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COMMENT        #29=(CFSignal (CObList) "hs1218" 9 0 0 221 1680 0 (CStringList) 
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COMMENT                          "Currently local object. Original author: User" 1) 
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COMMENT                          "Created: 07/06/16 02:29PM" 1) 
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FEATURES             Location/Qualifiers
     CDS             4988..5845
                     /vntifkey="4"
                     /label=Amp-R
     misc_feature    6019..6607
                     /vntifkey="21"
                     /label=Origin
     misc_feature    4050..4095
                     /vntifkey="21"
                     /label=additional\AAV\sequences
     repeat_region   1..168
                     /vntifkey="34"
                     /label=5'\ITR
     repeat_region   complement(4058..4225)
                     /vntifkey="34"
                     /label=3'\ITR
     misc_feature    197..208
                     /vntifkey="21"
                     /label=MCS\5'\end
     intron          2567..2739
                     /vntifkey="15"
                     /label=Intron
     CDS             2752..3783
                     /vntifkey="4"
                     /label=iCre
     primer          complement(2768..2788)
                     /vntifkey="27"
                     /label=oEMS5913
     enhancer        221..1680
                     /vntifkey="9"
                     /label=hs1218
     promoter        1681..2558
                     /vntifkey="29"
                     /label=Hspa1a\Prom\(aka\hsp68)
     mutation        1742..1742
                     /vntifkey="62"
                     /label=C-->G
     mutation        1740..1740
                     /vntifkey="62"
                     /label=T-->A
     primer          complement(572..593)
                     /vntifkey="27"
                     /label=oEMS5930
BASE COUNT     1811 a      1769 c      1755 g      1700 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct 
      181 aggaagatcg gaattcccta ggccggccac gcgtggcgcg tgctgctgta tagaatgttc 
      241 aagattctac gtgctttgtg aatcagtttt cagtatgctt caaatccatc aagggaaaat 
      301 gagggccatg attcttccaa atggaatcat taactaagtt tgaaagctca gagaattaaa 
      361 ccaagtttgt catgacctca gtttgaaaaa ttcagagaat taaactgagt ctcttgtgag 
      421 acagagaaca agtagaaact aggcttcaga aaggaagtaa tagtagaggc cactgaagta 
      481 agtcaccatt gacctatgtc cctctcaatg aagtttcttc tttacagtta gttcctgcag 
      541 aaaaatatat tcagtgcaat atattagtta tgggtccaac agcacagttc aaaacatgtg 
      601 tagctgatgt atgttttccc ccaaaaatac aagtattggg ccgttcattg tcatctacac 
      661 aaagaactga gtcctaaaat cctttactca actaaattct taataagtaa actgagacta 
      721 accctctaat accagatttg ttaaacaaca tgatttaggt ccttgctatt catgcacctt 
      781 gtacaactaa aatgactggt aaagctagaa tcatgaaaga gtcattttat gtagctgcta 
      841 aattcctctc aaatctcctt gcaacttaac gagtaaagaa agctcaatgt gtaatgccgg 
      901 ctaatgtggt ttatgctaga aaatagtatc aacctatctt tgtagcttat ctaagccagg 
      961 ataatacttt tagcctttat agtagaccca actccatttt ttcaactctt gactatgtat 
     1021 ttcctgaaat ccttttccaa agagttaaat tggatgtgaa atctgtacta ggctatggag 
     1081 ctgtctttgt tctaagtctc gccagcaaag cctcgcctct gtatcacttt cattgcgttc 
     1141 tgattgagct tgacagtgtg acaggaaagc aaagggaggc aattccaaac tgaatgagag 
     1201 tctttttcag ccacacagca gccttctcct tagcccctaa gaaaaagagg taacagatgc 
     1261 cgatagcaaa gcatccctaa caaagtctta actttgatgc aggcagaaga agaaaaaaat 
     1321 gtaaaagatt gtctcagcgc aggaaaacct tgaatggaac acaaaggaag caaaatgaga 
     1381 aaaatcgtgg ttcagaaaat ggctgggaga gttatgtagc tatcgtgcgt ttaagaaatt 
     1441 attttagtct caacaagagg ttaggatctg cagaatctgc tctgccaggt atggcacaac 
     1501 tggaatagtt gcaatatatg gggcccgagc aaaaggtgat cagtgatgct gtgattgctt 
     1561 ggcatggagg atgttcatga tgaatgccct gtcccttggg tggaaagtaa aactccattg 
     1621 aaatgacaac tttatagtat agccattacc tttgtactct ggggagtaga tcttttctga 
     1681 gagctccagg aacatccaaa ctgagcagcc ggggtccccc ccacccccca ccccgcccca 
     1741 cgcggcaact ttgagcctgt gctgggacag agcctctagt tcctaaatta gtccatgagg 
     1801 tcagaggcag cactgccatt gtaacgcgat tggagaggat cacgtcaccg gacacgcccc 
     1861 caggcatctc cctgggtctc ctaaacttgg cggggagaag ttttagccct taagttttag 
     1921 cctttaaccc ccatattcag aactgtgcga gttggcgaaa ccccacaaat cacaacaaac 
     1981 tgtacacaac accgagctag aggtgatctt tcttgtccat tccacacagg ccttagtaat 
     2041 gcgtcgccat agcaacagtg tcactagtag caccagcact tccccacacc ctccccctca 
     2101 ggaatccgta ctctccagtg aaccccagaa acctctggag agttctggac aagggcggaa 
     2161 cccacaactc cgattactca agggaggcgg ggaagctcca ccagacgcga aactgctgga 
     2221 agattcctgg ccccaaggcc tcctccggct cgctgattgg cccagcggag agtgggcggg 
     2281 gccggtgaag actccttaaa ggcgcagggc ggcgagcagg tcaccagacg ctgacagcta 
     2341 ctcagaacca aatctggttc catccagaga caagcgaaga caagagaagc agagcgagcg 
     2401 gcgcgttccc gatcctcggc caggaccagc cttccccaga gcatccctgc cgcggagcgc 
     2461 aaccttccca ggagcatccc tgccgcggag cgcaactttc cccggagcat ccacgccgcg 
     2521 gagcgcagcc ttccagaagc agagcgcggc gccatggcgg cgcgccgaag ttggtcgtga 
     2581 ggcactgggc aggtaagtat caaggttaca agacaggttt aaggagacca atagaaactg 
     2641 ggcttgtcga gacagagaag actcttgcgt ttctgatagg cacctattgg tcttactgac 
     2701 atccactttg cctttctctc cacaggtgtc cactcccagg cggccgccac catgtccaac 
     2761 ctgctgactg tgcaccaaaa cctgcctgcc ctccctgtgg atgccacctc tgatgaagtc 
     2821 aggaagaacc tgatggacat gttcagggac aggcaggcct tctctgaaca cacctggaag 
     2881 atgctcctgt ctgtgtgcag atcctgggct gcctggtgca agctgaacaa caggaaatgg 
     2941 ttccctgctg aacctgagga tgtgagggac tacctcctgt acctgcaagc cagaggcctg 
     3001 gctgtgaaga ccatccaaca gcacctgggc cagctcaaca tgctgcacag gagatctggc 
     3061 ctgcctcgcc cttctgactc caatgctgtg tccctggtga tgaggagaat cagaaaggag 
     3121 aatgtggatg ctggggagag agccaagcag gccctggcct ttgaacgcac tgactttgac 
     3181 caagtcagat ccctgatgga gaactctgac agatgccagg acatcaggaa cctggccttc 
     3241 ctgggcattg cctacaacac cctgctgcgc attgccgaaa ttgccagaat cagagtgaag 
     3301 gacatctccc gcaccgatgg tgggagaatg ctgatccaca ttggcaggac caagaccctg 
     3361 gtgtccacag ctggtgtgga gaaggccctg tccctggggg ttaccaagct ggtggagaga 
     3421 tggatctctg tgtctggtgt ggctgatgac cccaacaact acctgttctg ccgggtcaga 
     3481 aagaatggtg tggctgcccc ttctgccacc tcccaactgt ccacccgggc cctggaaggg 
     3541 atctttgagg ccacccaccg cctgatctat ggtgccaagg atgactctgg gcagagatac 
     3601 ctggcctggt ctggccactc tgccagagtg ggtgctgcca gggacatggc cagggctggt 
     3661 gtgtccatcc ctgaaatcat gcaggctggt ggctggacca atgtgaacat agtgatgaac 
     3721 tacatcagaa acctggactc tgagactggg gccatggtga ggctgctcga ggatggggac 
     3781 tgagcggccg cgatcgcaga catgataaga tacattgatg agtttggaca aaccacaact 
     3841 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta 
     3901 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag 
     3961 gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtagt 
     4021 cgaaattccc gataaggatc ttcctagagc atggctacgt agataagtag catggcgggt 
     4081 taatcattaa ctacaaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc 
     4141 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg 
     4201 cctcagtgag cgagcgagcg cgcagcctta attaacctaa ttcactggcc gtcgttttac 
     4261 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc 
     4321 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc 
     4381 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg 
     4441 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt 
     4501 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc 
     4561 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg 
     4621 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg 
     4681 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct 
     4741 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg 
     4801 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg 
     4861 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc 
     4921 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag 
     4981 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg 
     5041 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt 
     5101 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt 
     5161 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt 
     5221 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa 
     5281 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag 
     5341 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac 
     5401 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac 
     5461 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac 
     5521 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac 
     5581 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact 
     5641 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg 
     5701 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt 
     5761 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat 
     5821 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta 
     5881 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa 
     5941 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 
     6001 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 
     6061 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 
     6121 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc 
     6181 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 
     6241 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 
     6301 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 
     6361 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 
     6421 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 
     6481 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 
     6541 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 
     6601 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 
     6661 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga 
     6721 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga 
     6781 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg 
     6841 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt 
     6901 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt 
     6961 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc 
     7021 cagatttaat taagg 
//
