LOCUS Exported 3090 bp ds-DNA circular SYN 21-SEP-2016 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pUK21_sequenced SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3090) AUTHORS . TITLE Direct Submission JOURNAL Exported Wednesday, Sep 21, 2016 from SnapGene 3.2.1 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3090 /organism="pUK21-deltaBB" /mol_type="other DNA" /label=source:pUK21 /note="source:pUK21" misc_feature 179..208 /label=Anderson Promoter (lacI binding site) /note="Anderson Promoter (lacI binding site)" misc_feature 188..210 /label=M13_pUC_rev_primer /note="M13_pUC_rev_primer" misc_feature 209..227 /label=M13_reverse_primer /note="M13_reverse_primer" misc_feature 245..356 /label=MCS /note="MCS" misc_feature 351..367 /label=pBluescriptSK_primer /note="pBluescriptSK_primer" promoter complement(382..400) /label=T7_promoter /note="T7_promoter" misc_feature 404..559 /label=lacZ_a /note="lacZ_a" promoter complement(407..423) /label=M13_forward20_primer /note="M13_forward20_primer promoter" misc_feature complement(416..438) /label=M13_pUC_fwd_primer /note="M13_pUC_fwd_primer" misc_feature 1149..1171 /label=pGEX_3_primer /note="pGEX_3_primer" CDS 1369..2178 /codon_start=1 /label=ORF frame 2 /note="ORF frame 2" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" gene 1369..2178 /gene="KanR2" /label=KanR2 /note="KanR2" rep_origin 2305..2924 /label=pBR322_origin /note="pBR322_origin" ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 121 tcactcatta ggcaccccag gctttacact ttatgcttcc gcggctcgta tgttgtgtgg 181 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccactag 241 tccgaggcct cgagatctat cgatgcatgc catggtaccc gggagctcga attcgaagct 301 tctgcagacg cgtcgacgtc atatggatcc gatatcgccg tggcggccgc tctagaacta 361 gtggatcgat ccccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt 421 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc 481 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt 541 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg 601 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag 661 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc 721 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc 781 tctaaatcgg ggtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc 841 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact ctatctcggg 901 ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa aaaatgagct 961 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg 1021 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac 1081 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt 1141 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag 1201 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatggggg ggggggggaa 1261 agccacgttg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca 1321 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt 1381 caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat 1441 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag 1501 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca 1561 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat 1621 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca 1681 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg 1741 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta 1801 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt 1861 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg 1921 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt 1981 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac 2041 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg 2101 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg 2161 ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg 2221 ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttttcgttcc 2281 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 2341 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 2401 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 2461 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 2521 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 2581 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 2641 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 2701 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 2761 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 2821 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 2881 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 2941 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 3001 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc 3061 gcagcgagtc agtgagcgag gaagcggaag //