LOCUS ISI 4146 bp ds-DNA circular SYN 06-06-2016 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4146) AUTHORS . TITLE Direct Submission JOURNAL Exported from SnapGene Viewer 2.8.3 to Vector NTI(R) format http://www.snapgene.com COMMENT VNTDATE|771897600| COMMENT VNTDBDATE|771897600| COMMENT VNTNAME|ISI| FEATURES Location/Qualifiers primer_bind complement(10..26) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 500..604 /gene="bla" /label=AmpR promoter CDS 605..1465 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" rep_origin 1636..2224 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 2512..2533 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 2548..2578 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2586..2602 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2610..2626 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 2721..2754 /label=FRT (minimal) /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="supports FLP-mediated excision but not integration (Turan and Bode, 2011)" oriT 2761..2870 /label=oriT /note="incP origin of transfer" protein_bind 4038..4084 /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." ORIGIN 1 ttctttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca 61 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg 121 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta 181 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat 241 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc 301 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag 361 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt 421 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg 481 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa 541 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa 601 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct 661 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg 721 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg 781 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt 841 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga 901 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga 961 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac 1021 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg 1081 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac 1141 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct 1201 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct 1261 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg 1321 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat 1381 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg 1441 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat 1501 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct 1561 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa 1621 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa 1681 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc 1741 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta 1801 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct 1861 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg 1921 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag 1981 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc 2041 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg 2101 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt 2161 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg 2221 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca 2281 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg 2341 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc 2401 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 2461 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag 2521 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg 2581 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgaact 2641 cgtcctcggt tcccggcgat cctctggaga ttatattacc ctgttatccc taattccggg 2701 gatccgtcga cctgcagttc gaagttccta ttctctagaa agtataggaa cttcgaagtt 2761 cccgccagcc tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag 2821 tgaagaagga acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc 2881 gttggataca ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga 2941 aaaaggatgg atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg 3001 aaaatgcagc tcacggtaac tgatgccgta tttgcagtac cagcgtacgg cccacagaat 3061 gatgtcacgc tgaaaatgcc ggcctttgaa tgggttcatg tgcagctcca taagcaaaag 3121 gggatgataa gtttatcacc accgactatt tgcaacagtg ccgttgatcg tgctatgatc 3181 gactgatgtc atcagcggtg gagtgcaatg tcatgaggga agcggtgatc gccgaagtat 3241 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg 3301 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt 3361 tgctggttac ggtgaccgta aggcttgatg aaaacaacgc ggcgagcttt gatcaacgac 3421 ctttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca 3481 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg 3541 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg 3601 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg 3661 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct 3721 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt 3781 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg 3841 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg 3901 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg 3961 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataagatgcc gctcgccagt 4021 cgattggctg agctcataag ttcctattcc gaagttccta ttctctagaa agtataggaa 4081 cttcgaagca gctccagcct acacattacc ctgttatccc taataatcgt ccacttgccg 4141 tcatgc //