LOCUS       pChlr-pNEB193+Tn10.xdna    3619 bp      DNA     circular   2/1/17
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=Ligation of : PCR(pACYCDuet-1.xdna) [946  nt]
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    complement(1433..2089)
                     /label=cat
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    complement(2968..3587)
                     /label=pBR322 ori
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="pBR322 ori" - \ 
     misc_feature    complement(2594..2616)
                     /label=M13 pUC R
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="M13 pUC R" - \ 
     misc_feature    2453..2475
                     /label=M13 pUC F
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="M13 pUC F" - 364..386 \ 
     primer_bind     2467..2484
                     /label=M13-fwd
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     complement(2577..2597)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     rep_origin      complement(2965..3593)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             complement(2328..2396)
                     /label=LacZ alpha
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_binding    complement(2603..2625)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     Promoter        complement(2630..2659)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    52..1390
                     /label=Tn10 insert
                     /SerialCloner_Color=&hFF6666
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
ORIGIN
        1 ctttcgaatt tctgccattc atccgcttat tatcacttat tcaggcgtag cAACCAGGCG 
       61 CTGATGAATC CCCTAATGAT TTTGGTAAAA ATCATTAAGT TAAGGTGGAT ACACATCTTG 
      121 TCATATGATC AAATGGTTTC GCGAAAAATC AATAATCAGA CAACAAGATG TGCGAACTCG 
      181 ATATTTTACA CGACTCTCTT TACCAATTCT GCCCCGAATT ACACTTAAAA CGACTCAACA 
      241 GCTTAACGTT GGCTTGCCAC GCATTACTTG ACTGTAAAAC TCTCACTCTT ACCGAACTTG 
      301 GCCGTAACCT GCCAACCAAA GCGAGAACAA AACATAACAT CAAACGAATC GACCGATTGT 
      361 TAGGTAATCG TCACCTCCAC AAAGAGCGAC TCGCTGTATA CCGTTGGCAT GCTAGCTTTA 
      421 TCTGTTCGGG CAATACGATG CCCATTGTAC TTGTTGACTG GTCTGATATT CGTGAGCAAA 
      481 AACGACTTAT GGTATTGCGA GCTTCAGTCG CACTACACGG TCGTTCTGTT ACTCTTTATG 
      541 AGAAAGCGTT CCCGCTTTCA GAGCAATGTT CAAAGAAAGC TCATGACCAA TTTCTAGCCG 
      601 ACCTTGCGAG CATTCTACCG AGTAACACCA CACCGCTCAT TGTCAGTGAT GCTGGCTTTA 
      661 AAGTGCCATG GTATAAATCC GTTGAGAAGC TGGGTTGGTA CTGGTTAAGT CGAGTAAGAG 
      721 GAAAAGTACA ATATGCAGAC CTAGGAGCGG AAAACTGGAA ACCTATCAGC AACTTACATG 
      781 ATATGTCATC TAGTCACTCA AAGACTTTAG GCTATAAGAG GCTGACTAAA AGCAATCCAA 
      841 TCTCATGCCA AATTCTATTG TATAAATCTC GCTCTAAAGG CCGAAAAAAT CAGCGCTCGA 
      901 CACGGACTCA TTGTCACCAC CCGTCACCTA AAATCTACTC AGCGTCGGCA AAGGAGCCAT 
      961 GGGTTCTAGC AACTAACTTA CCTGTTGAAA TTCGAACACC CAAACAACTT GTTAATATCT 
     1021 ATTCGAAGCG AATGCAGATT GAAGAAACCT TCCGAGACTT GAAAAGTCCT GCCTACGGAC 
     1081 TAGGCCTACG CCATAGCCGA ACGAGCAGCT CAGAGCGTTT TGATATCATG CTGCTAATCG 
     1141 CCCTGATGCT TCAACTAACA TGTTGGCTTG CGGGCGTTCA TGCTCAGAAA CAAGGTTGGG 
     1201 ACAAGCACTT CCAGGCTAAC ACAGTCAGAA ATCGAAACGT ACTCTCAACA GTTCGCTTAG 
     1261 GCATGGAAGT TTTGCGGCAT TCTGGCTACA CAATAACAAG GGAAGACTTA CTCGTGGCTG 
     1321 CAACCCTACT AGCTCAAAAT TTATTCACAC ATGGTTACGC TTTGGGGAAA TTATGAGGGG 
     1381 ATCTCTCAGA accaggcgtt taagggcacc aataactgcc ttaaaaaaat tacgccccgc 
     1441 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat 
     1501 cacagacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat 
     1561 aatatttgcc catagtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat 
     1621 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc 
     1681 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta 
     1741 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct 
     1801 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca 
     1861 ttgccatacg gaactccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg 
     1921 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa 
     1981 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat 
     2041 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct 
     2101 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat 
     2161 ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc 
     2221 agggcttccc ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg 
     2281 tcacaAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGCCA TTCGCCATTC 
     2341 AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG 
     2401 GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA 
     2461 CGACGTTGTA AAACGACGGC CAGTGAATTC GAGCTCGGTA CCCGGGGGCG CGCCGGATCC 
     2521 TTAATTAAGT CTAGAGTCGA CTGTTTAAAC CTGCAGGCAT GCAAGCTTGG CGTAATCATG 
     2581 GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC 
     2641 CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC 
     2701 GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT 
     2761 CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC 
     2821 TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     2881 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     2941 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     3001 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     3061 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     3121 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCAAAG 
     3181 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     3241 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     3301 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     3361 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     3421 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     3481 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     3541 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     3601 TGACGCTCAG TGGAACGAA
//