LOCUS pDEST-FRT-TO-3xF 10795 bp DNA circular 18-JAN-2019 FEATURES Location/Qualifiers primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 769..789 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" protein_bind 820..838 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 841..859 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" primer_bind 869..893 /label=LNCX /note="Human CMV promoter, forward primer" CDS 1034..1057 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" protein_bind 1058..1082 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(6682..6705) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" primer_bind complement(6747..6764) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6753..6977 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(7110..7129) /label=F1ori-R /note="F1 origin, reverse primer" protein_bind 7261..7308 /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." CDS 7316..8338 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGDFSESLTYCISRRAQGVTLQDL PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYH WQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSE AMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQS LVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPST RPRAKE" polyA_signal 8468..8589 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8505..8524) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8559..8578 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8638..8654) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8638..8654) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8651..8673) /label="M13/pUC Reverse" /note="In lacZ gene" protein_bind 8662..8678 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8686..8716) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8731..8752 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8869..8886) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9040..9628) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9120..9139) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9799..10659) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" primer_bind 10422..10441 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10660..10764) /gene="bla" /label=AmpR promoter CDS 1088..6676 /note="BRCA1 (siRNA-resistant)" /translation="MDLSALRVEEVQNVINAMQKILECPICLELIKEPVSTKCDHIFC KFCMLKLLNQKKGPSQCPLCKNDITKRSLQESTRFSQLVEELLKIICAFQLDTGLEYA NSYNFAKKENNSPEHLKDEVSIIQSMGYRNRAKRLLQSEPENPSLQETSLSVQLSNLG TVRTLRTKQRIQPQKTSVYIELGSDSSEDTVNKATYCSVGDQELLQITPQGTRDEISL DSAKKAACEFSETDVTNTEHHQPSNNDLNTTEKRAAERHPEKYQGSSVSNLHVEPCGT NTHASSLQHENSSLLLTKDRMNVEKAEFCNKSKQPGLARSQHNRWAGSKETCNDRRTP STEKKVDLNADPLCERKEWNKQKLPCSENPRDTEDVPWITLNSSIQKVNEWFSRSDEL LGSDDSHDGESESNAKVADVLDVLNEVDEYSGSSEKIDLLASDPHEALICKSERVHSK SVESNIEDKIFGKTYRKKASLPNLSHVTENLIIGAFVTEPQIIQERPLTNKLKRKRRP TSGLHPEDFIKKADLAVQKTPEMINQGTNQTEQNGQVMNITNSGHENKTKGDSIQNEK NPNPIESLEKESAFKTKAEPISSSISNMELELNIHNSKAPKKNRLRRKSSTRHIHALE LVVSRNLSPPNCTELQIDSCSSSEEIKKKKYNQMPVRHSRNLQLMEGKEPATGAKKSN KPNEQTSKRHDSDTFPELKLTNAPGSFTKCSNTSELKEFVNPSLPREEKEEKLETVKV SNNAEDPKDLMLSGERVLQTERSVESSSISLVPGTDYGTQESISLLEVSTLGKAKTEP NKCVSQCAAFENPKGLIHGCSKDNRNDTEGFKYPLGHEVNHSRETSIEMEESELDAQY LQNTFKVSKRQSFALFSNPGNAEEECATFSAHSGSLKKQSPKVTFECEQKEENQGKNE SNIKPVQTVNITAGFPVVGQKDKPVDNAKCSIKGGSRFCLSSQFRGNETGLITPNKHG LLQNPYRIPPLFPIKSFVKTKCKKNLLEENFEEHSMSPEREMGNENIPSTVSTISRNN IRENVFKGASSSNINEVGSSTNEVGSSINEIGSSDENIQAELGRNRGPKLNAMLRLGV LQPEVYKQSLPGSNCKHPEIKKQEYEEVVQTVNTDFSPYLISDNLEQPMGSSHTSQVC SETPDDLLDDGEIKEDTSFAENDIKESSAVFSKSVQRGELSRSPSPFTHTHLAQGYRR GAKKLESSEENLSSEDEELPCFQHLLFGKVNNIPSQSTRHSTVATECLSKNTEENLLS LKNSLNDCSNQVILAKASQEHHLSEETKCSASLFSSQCSELEDLTANTNTQDPFLIGS SKQMRHQSESQGVGLSDKELVSDDEERGTGLEENNQEEQSMDSNLGEAASGCESETSV SEDCSGLSSQSDILTTQQRDTMQHNLIKLQQEMAELEAVLEQHGSQPSNSYPSIISDS SALEDLRNPEQSTSEKAVLTSQKSSEYPISQNPEGLSADKFEVSADSSTSKNKEPGVE RSSPSKCPSLDDRWYMHSCSGSLQNRNYPSQEELIKVVDVEEQQLEESGPHDLTETSY LPRQDLEGTPYLESGISLFSDDPESDPSEDRAPESARVGNIPSSTSALKVPQLKVAES AQGPAAAHTTDTAGYNAMEESVSREKPELTASTERVNKRMSMVVSGLTPEEFMLVYKF ARKHHITLTNLITEETTHVVMKTDAEFVCERTLKYFLGIAGGKWVVSYFWVTQSIKER KMLNEHDFEVRGDVVNGRNHQGPKRARESQDRKIFRGLEICCYGPFTNMPTDQLEWMV QLCGASVVKELSSFTLGTGVHPIVVVQPDAWTEDNGFHAIGQMCEAPVVTREWVLDSV ALYQCQELDTYLIPQIPHSHY" CDS 989..6676 /note="3xFLAG-BRCA1 siR" /translation="MDYKDHDGDTYDHDIDYKDDDDKTSLYKKAGFTMDLSALRVEEV QNVINAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNDIT KRSLQESTRFSQLVEELLKIICAFQLDTGLEYANSYNFAKKENNSPEHLKDEVSIIQS MGYRNRAKRLLQSEPENPSLQETSLSVQLSNLGTVRTLRTKQRIQPQKTSVYIELGSD SSEDTVNKATYCSVGDQELLQITPQGTRDEISLDSAKKAACEFSETDVTNTEHHQPSN NDLNTTEKRAAERHPEKYQGSSVSNLHVEPCGTNTHASSLQHENSSLLLTKDRMNVEK AEFCNKSKQPGLARSQHNRWAGSKETCNDRRTPSTEKKVDLNADPLCERKEWNKQKLP CSENPRDTEDVPWITLNSSIQKVNEWFSRSDELLGSDDSHDGESESNAKVADVLDVLN EVDEYSGSSEKIDLLASDPHEALICKSERVHSKSVESNIEDKIFGKTYRKKASLPNLS HVTENLIIGAFVTEPQIIQERPLTNKLKRKRRPTSGLHPEDFIKKADLAVQKTPEMIN QGTNQTEQNGQVMNITNSGHENKTKGDSIQNEKNPNPIESLEKESAFKTKAEPISSSI SNMELELNIHNSKAPKKNRLRRKSSTRHIHALELVVSRNLSPPNCTELQIDSCSSSEE IKKKKYNQMPVRHSRNLQLMEGKEPATGAKKSNKPNEQTSKRHDSDTFPELKLTNAPG SFTKCSNTSELKEFVNPSLPREEKEEKLETVKVSNNAEDPKDLMLSGERVLQTERSVE SSSISLVPGTDYGTQESISLLEVSTLGKAKTEPNKCVSQCAAFENPKGLIHGCSKDNR NDTEGFKYPLGHEVNHSRETSIEMEESELDAQYLQNTFKVSKRQSFALFSNPGNAEEE CATFSAHSGSLKKQSPKVTFECEQKEENQGKNESNIKPVQTVNITAGFPVVGQKDKPV DNAKCSIKGGSRFCLSSQFRGNETGLITPNKHGLLQNPYRIPPLFPIKSFVKTKCKKN LLEENFEEHSMSPEREMGNENIPSTVSTISRNNIRENVFKGASSSNINEVGSSTNEVG SSINEIGSSDENIQAELGRNRGPKLNAMLRLGVLQPEVYKQSLPGSNCKHPEIKKQEY EEVVQTVNTDFSPYLISDNLEQPMGSSHTSQVCSETPDDLLDDGEIKEDTSFAENDIK ESSAVFSKSVQRGELSRSPSPFTHTHLAQGYRRGAKKLESSEENLSSEDEELPCFQHL LFGKVNNIPSQSTRHSTVATECLSKNTEENLLSLKNSLNDCSNQVILAKASQEHHLSE ETKCSASLFSSQCSELEDLTANTNTQDPFLIGSSKQMRHQSESQGVGLSDKELVSDDE ERGTGLEENNQEEQSMDSNLGEAASGCESETSVSEDCSGLSSQSDILTTQQRDTMQHN LIKLQQEMAELEAVLEQHGSQPSNSYPSIISDSSALEDLRNPEQSTSEKAVLTSQKSS EYPISQNPEGLSADKFEVSADSSTSKNKEPGVERSSPSKCPSLDDRWYMHSCSGSLQN RNYPSQEELIKVVDVEEQQLEESGPHDLTETSYLPRQDLEGTPYLESGISLFSDDPES DPSEDRAPESARVGNIPSSTSALKVPQLKVAESAQGPAAAHTTDTAGYNAMEESVSRE KPELTASTERVNKRMSMVVSGLTPEEFMLVYKFARKHHITLTNLITEETTHVVMKTDA EFVCERTLKYFLGIAGGKWVVSYFWVTQSIKERKMLNEHDFEVRGDVVNGRNHQGPKR ARESQDRKIFRGLEICCYGPFTNMPTDQLEWMVQLCGASVVKELSSFTLGTGVHPIVV VQPDAWTEDNGFHAIGQMCEAPVVTREWVLDSVALYQCQELDTYLIPQIPHSHY" CDS 992..1057 /note="3xFLAG" /translation="DYKDHDGDTYDHDIDYKDDDDK" misc_feature 5368..5386 /note="siRNA resistant" source 1..10795 /dnas_title="pDEST-FRT-TO-3xFLAG-BRCA1" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc 841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga 901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga 961 ctctagcgtt taaacttaag cttccaccat ggactacaaa gaccatgacg gtgatacata 1021 tgatcatgac atcgattaca aggatgacga tgacaagaca agtttgtaca aaaaagcagg 1081 cttcaccATG GATTTATCTG CTCTTCGCGT TGAAGAAGTA CAAAATGTCA TTAATGCTAT 1141 GCAGAAAATC TTAGAGTGTC CCATCTGTCT GGAGTTGATC AAGGAACCTG TCTCCACAAA 1201 GTGTGACCAC ATATTTTGCA AATTTTGCAT GCTGAAACTT CTCAACCAGA AGAAAGGGCC 1261 TTCACAGTGT CCTTTATGTA AGAATGATAT AACCAAAAGG AGCCTACAAG AAAGTACGAG 1321 ATTTAGTCAA CTTGTTGAAG AGCTATTGAA AATCATTTGT GCTTTTCAGC TTGACACAGG 1381 TTTGGAGTAT GCAAACAGCT ATAATTTTGC AAAAAAGGAA AATAACTCTC CTGAACATCT 1441 AAAAGATGAA GTTTCTATCA TCCAAAGTAT GGGCTACAGA AACCGTGCCA AAAGACTTCT 1501 ACAGAGTGAA CCCGAAAATC CTTCCTTGCA GGAAACCAGT CTCAGTGTCC AACTCTCTAA 1561 CCTTGGAACT GTGAGAACTC TGAGGACAAA GCAGCGGATA CAACCTCAAA AGACGTCTGT 1621 CTACATTGAA TTGGGATCTG ATTCTTCTGA AGATACCGTT AATAAGGCAA CTTATTGCAG 1681 TGTGGGAGAT CAAGAATTGT TACAAATCAC CCCTCAAGGA ACCAGGGATG AAATCAGTTT 1741 GGATTCTGCA AAAAAGGCTG CTTGTGAATT TTCTGAGACG GATGTAACAA ATACTGAACA 1801 TCATCAACCC AGTAATAATG ATTTGAACAC CACTGAGAAG CGTGCAGCTG AGAGGCATCC 1861 AGAAAAGTAT CAGGGTAGTT CTGTTTCAAA CTTGCATGTG GAGCCATGTG GCACAAATAC 1921 TCATGCCAGC TCATTACAGC ATGAGAACAG CAGTTTATTA CTCACTAAAG ACAGAATGAA 1981 TGTAGAAAAG GCTGAATTCT GTAATAAAAG CAAACAGCCT GGCTTAGCAA GGAGCCAACA 2041 TAACAGATGG GCTGGAAGTA AGGAAACATG TAATGATAGG CGGACTCCCA GCACAGAAAA 2101 AAAGGTAGAT CTGAATGCTG ATCCCCTGTG TGAGAGAAAA GAATGGAATA AGCAGAAACT 2161 GCCATGCTCA GAGAATCCTA GAGATACTGA AGATGTTCCT TGGATAACAC TAAATAGCAG 2221 CATTCAGAAA GTTAATGAGT GGTTTTCCAG AAGTGATGAA CTGTTAGGTT CTGATGACTC 2281 ACATGATGGG GAGTCTGAAT CAAATGCCAA AGTAGCTGAT GTATTGGACG TTCTAAATGA 2341 GGTAGATGAA TATTCTGGTT CTTCAGAGAA AATAGACTTA CTGGCCAGTG ATCCTCATGA 2401 GGCTTTAATA TGTAAAAGTG AAAGAGTTCA CTCCAAATCA GTAGAGAGTA ATATTGAAGA 2461 CAAAATATTT GGGAAAACCT ATCGGAAGAA GGCAAGCCTC CCCAACTTAA GCCATGTAAC 2521 TGAAAATCTA ATTATAGGAG CATTTGTTAC TGAGCCACAG ATAATACAAG AGCGTCCCCT 2581 CACAAATAAA TTAAAGCGTA AAAGGAGACC TACATCAGGC CTTCATCCTG AGGATTTTAT 2641 CAAGAAAGCA GATTTGGCAG TTCAAAAGAC TCCTGAAATG ATAAATCAGG GAACTAACCA 2701 AACGGAGCAG AATGGTCAAG TGATGAATAT TACTAATAGT GGTCATGAGA ATAAAACAAA 2761 AGGTGATTCT ATTCAGAATG AGAAAAATCC TAACCCAATA GAATCACTCG AAAAAGAATC 2821 TGCTTTCAAA ACGAAAGCTG AACCTATAAG CAGCAGTATA AGCAATATGG AACTCGAATT 2881 AAATATCCAC AATTCAAAAG CACCTAAAAA GAATAGGCTG AGGAGGAAGT CTTCTACCAG 2941 GCATATTCAT GCGCTTGAAC TAGTAGTCAG TAGAAATCTA AGCCCACCTA ATTGTACTGA 3001 ATTGCAAATT GATAGTTGTT CTAGCAGTGA AGAGATAAAG AAAAAAAAGT ACAACCAAAT 3061 GCCAGTCAGG CACAGCAGAA ACCTACAACT CATGGAAGGT AAAGAACCTG CAACTGGAGC 3121 CAAGAAGAGT AACAAGCCAA ATGAACAGAC AAGTAAAAGA CATGACAGTG ATACTTTCCC 3181 AGAGCTGAAG TTAACAAATG CACCTGGTTC TTTTACTAAG TGTTCAAATA CCAGTGAACT 3241 TAAAGAATTT GTCAATCCTA GCCTTCCAAG AGAAGAAAAA GAAGAGAAAC TAGAAACAGT 3301 TAAAGTGTCT AATAATGCTG AAGACCCCAA AGATCTCATG TTAAGTGGAG AAAGGGTTTT 3361 GCAAACTGAA AGATCTGTAG AGAGTAGCAG TATTTCACTG GTACCTGGTA CTGATTATGG 3421 CACTCAGGAA AGTATCTCGT TACTGGAAGT TAGCACTCTA GGGAAGGCAA AAACAGAACC 3481 AAATAAATGT GTGAGTCAGT GTGCAGCATT TGAAAACCCC AAGGGACTAA TTCATGGTTG 3541 TTCCAAAGAT AATAGAAATG ACACAGAAGG CTTTAAGTAT CCATTGGGAC ATGAAGTTAA 3601 CCACAGTCGG GAAACAAGCA TAGAAATGGA AGAAAGTGAA CTTGATGCTC AGTATTTGCA 3661 GAATACATTC AAGGTTTCAA AGCGCCAGTC ATTTGCTCTG TTTTCAAATC CAGGAAATGC 3721 AGAAGAGGAA TGTGCAACAT TCTCTGCCCA CTCTGGGTCC TTAAAGAAAC AAAGTCCAAA 3781 AGTCACTTTT GAATGTGAAC AAAAGGAAGA AAATCAAGGA AAGAATGAGT CTAATATCAA 3841 GCCTGTACAG ACAGTTAATA TCACTGCAGG CTTTCCTGTG GTTGGTCAGA AAGATAAGCC 3901 AGTTGATAAT GCCAAATGTA GTATCAAAGG AGGCTCTAGG TTTTGTCTAT CATCTCAGTT 3961 CAGAGGCAAC GAAACTGGAC TCATTACTCC AAATAAACAT GGACTTTTAC AAAACCCATA 4021 TCGTATACCA CCACTTTTTC CCATCAAGTC ATTTGTTAAA ACTAAATGTA AGAAAAATCT 4081 GCTAGAGGAA AACTTTGAGG AACATTCAAT GTCACCTGAA AGAGAAATGG GAAATGAGAA 4141 CATTCCAAGT ACAGTGAGCA CAATTAGCCG TAATAACATT AGAGAAAATG TTTTTAAAGG 4201 AGCCAGCTCA AGCAATATTA ATGAAGTAGG TTCCAGTACT AATGAAGTGG GCTCCAGTAT 4261 TAATGAAATA GGTTCCAGTG ATGAAAACAT TCAAGCAGAA CTAGGTAGAA ACAGAGGGCC 4321 AAAATTGAAT GCTATGCTTA GATTAGGGGT TTTGCAACCT GAGGTCTATA AACAAAGTCT 4381 TCCTGGAAGT AATTGTAAGC ATCCTGAAAT AAAAAAGCAA GAATATGAAG AAGTAGTTCA 4441 GACTGTTAAT ACAGATTTCT CTCCATATCT GATTTCAGAT AACTTAGAAC AGCCTATGGG 4501 AAGTAGTCAT ACATCTCAGG TTTGTTCTGA GACACCTGAT GACCTGTTAG ATGATGGTGA 4561 AATAAAGGAA GATACTAGTT TTGCTGAAAA TGACATTAAG GAAAGTTCTG CTGTTTTTAG 4621 CAAAAGCGTC CAGAGAGGAG AGCTTAGCAG GAGTCCTAGC CCTTTCACCC ATACACATTT 4681 GGCTCAGGGT TACCGAAGAG GGGCCAAGAA ATTAGAGTCC TCAGAAGAGA ACTTATCTAG 4741 TGAGGATGAA GAGCTTCCCT GCTTCCAACA CTTGTTATTT GGTAAAGTAA ACAATATACC 4801 TTCTCAGTCT ACTAGGCATA GCACCGTTGC TACCGAGTGT CTGTCTAAGA ACACAGAGGA 4861 GAATTTATTA TCATTGAAGA ATAGCTTAAA TGACTGCAGT AACCAGGTAA TATTGGCAAA 4921 GGCATCTCAG GAACATCACC TTAGTGAGGA AACAAAATGT TCTGCTAGCT TGTTTTCTTC 4981 ACAGTGCAGT GAATTGGAAG ACTTGACTGC AAATACAAAC ACCCAGGATC CTTTCTTGAT 5041 TGGTTCTTCC AAACAAATGA GGCATCAGTC TGAAAGCCAG GGAGTTGGTC TGAGTGACAA 5101 GGAATTGGTT TCAGATGATG AAGAAAGAGG AACGGGCTTG GAAGAAAATA ATCAAGAAGA 5161 GCAAAGCATG GATTCAAACT TAGGTGAAGC AGCATCTGGG TGTGAGAGTG AAACAAGCGT 5221 CTCTGAAGAC TGCTCAGGGC TATCCTCTCA GAGTGACATT TTAACCACTC AGCAGAGGGA 5281 TACCATGCAA CATAACCTGA TAAAGCTCCA GCAGGAAATG GCTGAACTAG AAGCTGTGTT 5341 AGAACAGCAT GGGAGCCAGC CTTCTAACAG CTACCCTagt ATaATcAGTG ACTCTTCTGC 5401 CCTTGAGGAC CTGCGAAATC CAGAACAAAG CACATCAGAA AAAGCAGTAT TAACTTCACA 5461 GAAAAGTAGT GAATACCCTA TAAGCCAGAA TCCAGAAGGC CTTTCTGCTG ACAAGTTTGA 5521 GGTGTCTGCA GATAGTTCTA CCAGTAAAAA TAAAGAACCA GGAGTGGAAA GGTCATCCCC 5581 TTCTAAATGC CCATCATTAG ATGATAGGTG GTACATGCAC AGTTGCTCTG GGAGTCTTCA 5641 GAATAGAAAC TACCCATCTC AAGAGGAGCT CATTAAGGTT GTTGATGTGG AGGAGCAACA 5701 GCTGGAAGAG TCTGGGCCAC ACGATTTGAC GGAAACATCT TACTTGCCAA GGCAAGATCT 5761 AGAGGGAACC CCTTACCTGG AATCTGGAAT CAGCCTCTTC TCTGATGACC CTGAATCTGA 5821 TCCTTCTGAA GACAGAGCCC CAGAGTCAGC TCGTGTTGGC AACATACCAT CTTCAACCTC 5881 TGCATTGAAA GTTCCCCAAT TGAAAGTTGC AGAATCTGCC CAGGGTCCAG CTGCTGCTCA 5941 TACTACTGAT ACTGCTGGGT ATAATGCAAT GGAAGAAAGT GTGAGCAGGG AGAAGCCAGA 6001 ATTGACAGCT TCAACAGAAA GGGTCAACAA AAGAATGTCC ATGGTGGTGT CTGGCCTGAC 6061 CCCAGAAGAA TTTATGCTCG TGTACAAGTT TGCCAGAAAA CACCACATCA CTTTAACTAA 6121 TCTAATTACT GAAGAGACTA CTCATGTTGT TATGAAAACA GATGCTGAGT TTGTGTGTGA 6181 ACGGACACTG AAATATTTTC TAGGAATTGC GGGAGGAAAA TGGGTAGTTA GCTATTTCTG 6241 GGTGACCCAG TCTATTAAAG AAAGAAAAAT GCTGAATGAG CATGATTTTG AAGTCAGAGG 6301 AGATGTGGTC AATGGAAGAA ACCACCAAGG TCCAAAGCGA GCAAGAGAAT CCCAGGACAG 6361 AAAGATCTTC AGGGGGCTAG AAATCTGTTG CTATGGGCCC TTCACCAACA TGCCCACAGA 6421 TCAACTGGAA TGGATGGTAC AGCTGTGTGG TGCTTCTGTG GTGAAGGAGC TTTCATCATT 6481 CACCCTTGGC ACAGGTGTCC ACCCAATTGT GGTTGTGCAG CCAGATGCCT GGACAGAGGA 6541 CAATGGCTTC CATGCAATTG GGCAGATGTG TGAGGCACCT GTGGTGACCC GAGAGTGGGT 6601 GTTGGACAGT GTAGCACTCT ACCAGTGCCA GGAGCTGGAC ACCTACCTGA TACCCCAGAT 6661 CCCCCACAGC CACTACtgag acccagcttt cttgtacaaa gtggtctgac tcgagtctag 6721 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt 6781 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc 6841 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg 6901 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga 6961 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc 7021 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac 7081 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc 7141 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt 7201 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtaccta 7261 gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcct tggccaaaaa 7321 gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc 7381 cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg 7441 gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt 7501 ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggattt 7561 cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct 7621 gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc 7681 tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca 7741 atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca 7801 aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct 7861 ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa 7921 tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg 7981 ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga 8041 gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg 8101 ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt 8161 cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac 8221 tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga 8281 agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaatagca 8341 cgtactacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt 8401 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc 8461 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 8521 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 8581 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc 8641 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 8701 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 8761 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 8821 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 8881 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 8941 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 9001 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 9061 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 9121 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 9181 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 9241 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 9301 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 9361 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 9421 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 9481 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 9541 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 9601 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 9661 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 9721 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 9781 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 9841 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 9901 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 9961 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 10021 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 10081 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 10141 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 10201 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 10261 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 10321 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 10381 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 10441 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 10501 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 10561 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 10621 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 10681 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 10741 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc //