LOCUS       pBT296       5117 bp ss-DNA     circular SYN 09-Jul-2006
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     primer_bind     552..571
                     /note=PR 21 (pBS_552F)
     promoter        626..645
                     /note=T7
     misc_feature    complement(684..731)
                     /note=FRT5
     polyA_signal    complement(758..1029)
                     /note=HSV TK polyA
     CDS             complement(1204..1998)
                     /note=Neo/Kana
     primer_bind     1224..1241
                     /note=Neo.15 (Sequetech)
     primer_bind     1272..1289
                     /note=Neo.17 (Sequetech)
     primer_bind     complement(1273..1290)
                     /note=Neo.13 (Sequetech)
     primer_bind     complement(1284..1306)
                     /note=Shakti 5Seq3
     primer_bind     1656..1673
                     /note=NeoR (Sequetech)
     primer_bind     complement(1912..1929)
                     /note=NeoF (Sequetech)
     primer_bind     1919..1936
                     /note=Neo.11 (Sequetech)
     primer_bind     1956..1973
                     /note=Neo.19 (Sequetech)
     promoter        complement(2048..2350)
                     /note=SV40 promoter
     rep_origin      complement(2048..2183)
                     /note=SV40 ori
     primer_bind     2348..2372
                     /note=Shakti 3Seq1
     promoter        complement(2426..2523)
                     /note=Bacterial promoter
     misc_feature    complement(2536..2583)
                     /note=FRT5
     misc_feature    complement(2610..2830)
                     /note=attP
     misc_feature    complement(2848..2883)
                     /note=U
     promoter        complement(2931..2950)
                     /note=T3
     primer_bind     complement(3024..3043)
                     /note=PR 22 (pBS_884R)
     rep_origin      3191..4071
                     /note=ColE1\ORI
     CDS             complement(4132..4992)
                     /note=Amp
BASE COUNT      1238 A      1345 C      1337 G      1197 T         0 OTHER
ORIGIN      ?
        1 CACCTAAATT GTAAGCGTTA ATATTTTGTT AAAATTCGCG TTAAATTTTT GTTAAATCAG
       61 CTCATTTTTT AACCAATAGG CCGAAATCGG CAAAATCCCT TATAAATCAA AAGAATAGAC
      121 CGAGATAGGG TTGAGTGTTG TTCCAGTTTG GAACAAGAGT CCACTATTAA AGAACGTGGA
      181 CTCCAACGTC AAAGGGCGAA AAACCGTCTA TCAGGGCGAT GGCCCACTAC GTGAACCATC
      241 ACCCTAATCA AGTTTTTTGG GGTCGAGGTG CCGTAAAGCA CTAAATCGGA ACCCTAAAGG
      301 GAGCCCCCGA TTTAGAGCTT GACGGGGAAA GCCGGCGAAC GTGGCGAGAA AGGAAGGGAA
      361 GAAAGCGAAA GGAGCGGGCG CTAGGGCGCT GGCAAGTGTA GCGGTCACGC TGCGCGTAAC
      421 CACCACACCC GCCGCGCTTA ATGCGCCGCT ACAGGGCGCG TCCCATTCGC CATTCAGGCT
      481 GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA
      541 AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG
      601 TTGTAAAACG ACGGCCAGTG AATTGTAATA CGACTCACTA TAGGGCGAAT TGGGTACAGG
      661 CGCGCCCCTT ACTAGGCGGC CGCGAAGTTC CTATACCTTT TGAAGAATAG GAACTTCGGA
      721 ATAGGAACTT CTCTAGAGAT GATCCTGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC
      781 CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG
      841 AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC
      901 CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC
      961 TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA
     1021 GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC
     1081 AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT
     1141 CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC
     1201 CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC
     1261 GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC
     1321 ACGGGTAGCC AACGCTATGT CCTGATAGCG GTCCGCCACA CCCAGCCGGC CACAGTCGAT
     1381 GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT
     1441 CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG
     1501 CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG
     1561 AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC
     1621 AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG
     1681 GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC
     1741 TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG
     1801 CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG
     1861 AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG
     1921 TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA
     1981 TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA
     2041 AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG
     2101 CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC
     2161 TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT
     2221 TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG
     2281 TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT
     2341 TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT
     2401 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA
     2461 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC
     2521 TGAATAATCG CTAGCGAAGT TCCTATACCT TTTGAAGAAT AGGAACTTCG GAATAGGAAC
     2581 TTCGTCGACA AAGGATCCTT CTCGACTAGT CGCGCTCGCG CGACTGACGG TCGTAAGCAC
     2641 CCGCGTACGT GTCCACCCCG GTCACAACCC CTTGTGTCAT GTCGGCGACC CTACGCCCCC
     2701 AACTGAGAGA ACTCAAAGGT TACCCCAGTT GGGGCACTAC TCCCGAAAAC CGCTTCTGAC
     2761 CTGGGAAAAC GTGAAGCCCC GGGGCATCCG CTGAGGGTTG CCGCCGGGGC TTCGGTGTGT
     2821 CCGTCAGTAC TAGTCGAGAA AAGATCCGAT ATCCTTACGG AATACCACTT GCCACCTATC
     2881 ACCAGATCTG GATGCATGAT CTCCTATTTA AATCAGCTCC AGCTTTTGTT CCCTTTAGTG
     2941 AGGGTTAATT TCGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA
     3001 TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC
     3061 CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG
     3121 AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG
     3181 TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG
     3241 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA
     3301 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC
     3361 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC
     3421 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
     3481 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
     3541 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA
     3601 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
     3661 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC
     3721 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT
     3781 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT
     3841 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
     3901 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
     3961 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
     4021 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
     4081 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
     4141 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     4201 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     4261 AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     4321 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     4381 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     4441 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     4501 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     4561 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     4621 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     4681 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     4741 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     4801 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     4861 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     4921 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     4981 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     5041 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     5101 ATTTCCCCGA AAAGTGC
//
