LOCUS       pBT307                  7207 bp ss-DNA     circular SYN 09-Jul-2006
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     primer_bind     552..571
                     /note=PR 21 (pBS_552F)
     promoter        626..645
                     /note=T7
     misc_feature    complement(684..731)
                     /note=FRT5
     polyA_signal    complement(743..>969)
                     /note=bGH_pA
     primer_bind     955..972
                     /note=BGH_rev_primer
     CDS             complement(996..2834)
                     /note=Phi_C31o
     polyA_signal    complement(2887..3158)
                     /note=HSV TK polyA
     CDS             complement(3333..4127)
                     /note=Neo/Kana
     primer_bind     3353..3370
                     /note=Neo.15 (Sequetech)
     primer_bind     3401..3418
                     /note=Neo.17 (Sequetech)
     primer_bind     complement(3402..3419)
                     /note=Neo.13 (Sequetech)
     primer_bind     complement(3413..3435)
                     /note=Shakti 5Seq3
     primer_bind     3785..3802
                     /note=NeoR (Sequetech)
     primer_bind     complement(4041..4058)
                     /note=NeoF (Sequetech)
     primer_bind     4048..4065
                     /note=Neo.11 (Sequetech)
     primer_bind     4085..4102
                     /note=Neo.19 (Sequetech)
     promoter        complement(4177..4479)
                     /note=SV40 promoter
     rep_origin      complement(4177..4312)
                     /note=SV40 ori
     primer_bind     4477..4501
                     /note=Shakti 3Seq1
     promoter        complement(4555..4652)
                     /note=Bacterial promoter
     misc_feature    complement(4665..4712)
                     /note=FRT5
     misc_feature    complement(4728..4797)
                     /note=attP70
     misc_feature    complement(4798..4867)
                     /note=attP70
     misc_feature    complement(4868..4937)
                     /note=attP70
     misc_feature    complement(4944..4973)
                     /note=Unique Seq
     promoter        complement(5021..5040)
                     /note=T3
     primer_bind     complement(5114..5133)
                     /note=PR 22 (pBS_884R)
     rep_origin      5281..6161
                     /note=ColE1\ORI
     CDS             6224..7081
                     /note=Amp; Ampicillin resistance gene
BASE COUNT      1543 A      2042 C      1956 G      1666 T         0 OTHER
ORIGIN      ?
        1 CACCTAAATT GTAAGCGTTA ATATTTTGTT AAAATTCGCG TTAAATTTTT GTTAAATCAG
       61 CTCATTTTTT AACCAATAGG CCGAAATCGG CAAAATCCCT TATAAATCAA AAGAATAGAC
      121 CGAGATAGGG TTGAGTGTTG TTCCAGTTTG GAACAAGAGT CCACTATTAA AGAACGTGGA
      181 CTCCAACGTC AAAGGGCGAA AAACCGTCTA TCAGGGCGAT GGCCCACTAC GTGAACCATC
      241 ACCCTAATCA AGTTTTTTGG GGTCGAGGTG CCGTAAAGCA CTAAATCGGA ACCCTAAAGG
      301 GAGCCCCCGA TTTAGAGCTT GACGGGGAAA GCCGGCGAAC GTGGCGAGAA AGGAAGGGAA
      361 GAAAGCGAAA GGAGCGGGCG CTAGGGCGCT GGCAAGTGTA GCGGTCACGC TGCGCGTAAC
      421 CACCACACCC GCCGCGCTTA ATGCGCCGCT ACAGGGCGCG TCCCATTCGC CATTCAGGCT
      481 GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA
      541 AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG
      601 TTGTAAAACG ACGGCCAGTG AATTGTAATA CGACTCACTA TAGGGCGAAT TGGGTACAGG
      661 CGCGCCCCTT ACTAGGCGGC CGCGAAGTTC CTATACCTTT TGAAGAATAG GAACTTCGGA
      721 ATAGGAACTT CTCTAGGACG TCCATAGAGC CCACCGCATC CCCAGCATGC CTGCTATTGT
      781 CTTCCCAATC CTCCCCCTTG CTGTCCTGCC CCACCCCACC CCCCAGAATA GAATGACACC
      841 TACTCAGACA ATGCGATGCA ATTTCCTCAT TTTATTAGGA AAGGACAGTG GGAGTGGCAC
      901 CTTCCAGGGT CAAGGAAGGC ACGGGGGAGG GGCAAACAAC AGATGGCTGG CAACTAGAAG
      961 GCACAGTCGA GGCTGATCAG CGAGCTCTAG ATCTATCACA CTTTCCGCTT TTTCTTAGGG
     1021 GCGGCCACGT CCTCGGTGCC GTCCTGGGCG TCATCCTCGT CGTCGTCGGT GGGAGGCTTG
     1081 GCCCAGGTGA TGCTGGCTCT CTTCTCGATG GGGGTGCCCT GGCCCCTGCC GGTGGTGCTC
     1141 TTGGTCACCA CGATCTTATC CACGAACAGG CCCACGAACA CCCTCTTGTC GTCCACGCTG
     1201 GCTCTGCCCC ACCAGGACTT GGGGCCGGTG GGGTCGGCGT CGGCATCCTC GGGGAACCAC
     1261 TGATCCAGGG GCAGCTTAGG GGCCTCGGCG GCCTCCAGCT CGGCCAGTCT TTCCTCGGCG
     1321 CCCTGCTGTC TCAGGGTCAG GGCGGCCTGC TGCTTTCTGA AGTGCTTCCT GCCCACAGGG
     1381 CCGTCATAGG CTCCGGCGGC TCTGTCCTCG TACAGCTCCT CCAGGGCGTT CAGGGCGTCG
     1441 GCTCTCTCGG CCACCAGGTT GGCCCTCTCG CCGCTCTTCT CGGGGGCCTC GGTCAGCTTG
     1501 CCGAATCTTC TGGCGGCCTC CCACAGCAGG GCCAGGGTCT CCTCGTCGCC CTCGGCGTGC
     1561 CGGATCTTGT TGAAGATCCG CTCGGCCACG AACTTGTCCA GGGCGGCCAT GCTCACATTA
     1621 CAGGTGCCCT CGTGCTGGCC AGGGGCGCTG GGGTCCACCA CCTTTCTTCT CCGGCACCGG
     1681 TAGCTGTCCT TGATGCTCTC CTCGCCTCTC TTGCTGGTCA TCACGGCGCC ACACTCACAG
     1741 TACAGCTTGT CCATGGCGCT CAGGATGGCC TGGCCTCTGC TCAGGCCCTT GCCTCTGCCT
     1801 CTGCCGTCCA GCCAGGCCTG CAGCTCGTAC CACTCGGCAG GCTCGATGAT AGGGCCACAG
     1861 TCCAGCTCCA CAGGTCTCAG GGTGATGGGG TCTCTCTGGA TTCTGTAGCC CTCGATCTTG
     1921 GTGGTGGGGG TGCCGTCGGG CTTCTTCTTG TAGATCACCT CGGCGGCGAA GCCGGCGATC
     1981 CTAGGGTCCC TCAGGATTCT CATCACGGTG GCGGGGTCCC AGGCGCTGCT GGCGGTTTTC
     2041 TTGCCGATGG TCTCGCCTCT GGTGGGCACG GCGTCGGCGT CCATTCTCTT ACACAGGCCG
     2101 GTGATGCTGC CGGGGTGGAT GGCGGCCTGG CTGCCGGGCT TGAAAGGCAG GTGCTTGTGG
     2161 GTCTTGATCT CCCGCCACCA CCACCTGATC ACGTCGGGCT CAAACTCGAA GGGGCCGGTC
     2221 AGGGGGGTGG TGCTGTGGGC CAGCTTGTTG ATCACCACGT TCACCATCCT GCCGTTCCGG
     2281 GTGATCTCCT TGGTCTCGGA CACCAGCTCG AAGCCGTAGG GGGCCTTGCC GCCCACATAG
     2341 CCGCCCAGCT CCCTCTGCAG GTTCTTGGTG TCCAGGATCT TGGCGCTCTT CAGGCTGCTC
     2401 TCCTTGTGGC TGGCGTCCAG CCTCATGATC AGGTGGATCA GGTCCATCAC GTTGCCCTGT
     2461 CTAAAGACGC CTTCCTGGGT GGACACGATG GTCACGCCCA GGGCCAGCAG CTCGGACACG
     2521 ATAGGGATGG CGTCCATCAC CTTCAGCCTG CTGAACCGGG ACACGTCGTA CACGATGATC
     2581 ATGTTCAGCC TGCCGGCCCT ACACTCGTTC AGGATTCTCT CGAACTCGGG TCTCTCGGCG
     2641 GTGCCGAAGG CGCTGGTGCC AGGGGCCTCG CTGAAGTGGC CCACAAATCT GAATCTGCCG
     2701 CCGTCCCTCT CCACCTCTCT CTGCAGATCG GCGGCCTTAT CCTCGTTGGC GCTTCTCTGG
     2761 GTGGCGGGGC TGGCGGCGCT GCTGTTCTCT CTCTCCCGGC TCTGTCTGTC GTAGGCTCCG
     2821 GCGTAGGTAT CCATGGTGGC GGTTAACTTA ATTCCTGCAG GCTAGAGATG ATCCTGAGTA
     2881 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC
     2941 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG
     3001 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA
     3061 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC
     3121 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG
     3181 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC
     3241 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC
     3301 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG
     3361 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC
     3421 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGG
     3481 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG
     3541 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC
     3601 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA
     3661 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT
     3721 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC
     3781 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT
     3841 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA
     3901 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG
     3961 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC
     4021 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC
     4081 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT
     4141 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA
     4201 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG
     4261 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT
     4321 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC
     4381 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT
     4441 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG
     4501 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT
     4561 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT
     4621 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GAATAATCGC TAGCGAAGTT CCTATACCTT
     4681 TTGAAGAATA GGAACTTCGG AATAGGAACT TCGTCGACAA AGGATCCGTC GGCGACCCTA
     4741 CGCCCCCAAC TGAGAGAACT CAAAGGTTAC CCCAGTTGGG GCACTACTCC CGAAAACGTC
     4801 GGCGACCCTA CGCCCCCAAC TGAGAGAACT CAAAGGTTAC CCCAGTTGGG GCACTACTCC
     4861 CGAAAACGTC GGCGACCCTA CGCCCCCAAC TGAGAGAACT CAAAGGTTAC CCCAGTTGGG
     4921 GCACTACTCC CGAAAACGAT ATCCTTACGG AATACCACTT GCCACCTATC ACCAGATCTG
     4981 GATGCATGAT CTCCTATTTA AATCAGCTCC AGCTTTTGTT CCCTTTAGTG AGGGTTAATT
     5041 TCGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA
     5101 ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG
     5161 AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG
     5221 TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC
     5281 TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA
     5341 TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG
     5401 AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG
     5461 TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG
     5521 TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG
     5581 CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA
     5641 AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC
     5701 TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT
     5761 AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT
     5821 GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG
     5881 CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT
     5941 ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT
     6001 GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT
     6061 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG
     6121 GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT
     6181 AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT
     6241 GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC
     6301 GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG
     6361 CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC
     6421 GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG
     6481 GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA
     6541 GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA
     6601 TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT
     6661 CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG
     6721 CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA
     6781 ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA
     6841 CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT
     6901 TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT
     6961 CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA
     7021 ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC
     7081 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA
     7141 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA
     7201 AAAGTGC
//
