LOCUS       lentiGuide-Puro             9335 bp    DNA     circular     31-MAR-2014
FEATURES             Location/Qualifiers
     misc_feature    4141..4947
                     /note="AmpR"
     rep_origin      complement(5073..5688)
                     /note="pUC Ori"
     LTR             6036..6448
                     /note="RSV/5'LTR"
     misc_feature    10013..10130
                     /note="cPPT"
     rep_origin      3340..3770
                     /note="f1 Ori"
     misc_feature    6603..6967
                     /note="gag"
     misc_feature    7113..7354
                     /note="RRE"
     misc_feature    6499..6636
                     /note="psi"
     CDS             1277..1876
                     /note="Puromycin resistance"
     LTR             2551..2786
                     /note="3' LTR SIN"
     misc_RNA        1892..2480
                     /note="WPRE"
     polyA_signal    2863..2993
                     /note="SV40 polyA"
     promoter        7..1264
                     /note="EF-1a"
     promoter        7752..7996
                     /note="Human U6 Promoter"
     misc_feature    9882..9963
                     /note="chimeric RNA backbone 1 (BsmBI)"
     misc_binding    7997..9881
                     /note="Filler (digest with BsmBI to replace filler with
                     sgRNA oligos)"
ORIGIN      
          1 cccgggtgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct
         61 tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat
        121 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgatccggt gcctagagaa
        181 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
        241 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
        301 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
        361 gttatggccc ttgcgtgcct tgaattactt ccactggctg cagtacgtga ttcttgatcc
        421 cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc
        481 gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg
        541 gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg
        601 acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca
        661 cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac
        721 atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca
        781 agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc
        841 ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc
        901 tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc
        961 cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta
       1021 ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg
       1081 ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt
       1141 taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc
       1201 ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc
       1261 gtgacgtacg gccaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
       1321 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
       1381 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
       1441 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg ccgtggcggt
       1501 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
       1561 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
       1621 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggagtctcgc ccgaccacca
       1681 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
       1741 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
       1801 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
       1861 caagcccggt gcctgaacgc gttaagtcga caatcaacct ctggattaca aaatttgtga
       1921 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
       1981 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
       2041 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
       2101 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
       2161 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
       2221 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
       2281 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
       2341 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
       2401 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
       2461 cctttgggcc gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat
       2521 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
       2581 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
       2641 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
       2701 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
       2761 tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag
       2821 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
       2881 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
       2941 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
       3001 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
       3061 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
       3121 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc
       3181 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
       3241 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
       3301 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
       3361 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
       3421 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
       3481 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
       3541 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
       3601 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
       3661 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
       3721 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
       3781 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
       3841 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
       3901 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
       3961 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
       4021 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
       4081 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
       4141 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
       4201 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
       4261 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
       4321 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
       4381 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
       4441 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
       4501 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
       4561 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
       4621 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
       4681 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
       4741 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
       4801 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
       4861 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
       4921 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
       4981 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
       5041 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
       5101 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
       5161 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
       5221 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
       5281 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
       5341 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
       5401 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
       5461 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
       5521 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
       5581 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
       5641 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
       5701 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
       5761 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
       5821 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
       5881 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
       5941 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
       6001 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
       6061 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
       6121 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
       6181 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
       6241 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
       6301 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
       6361 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
       6421 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
       6481 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
       6541 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
       6601 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
       6661 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
       6721 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
       6781 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
       6841 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
       6901 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
       6961 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
       7021 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
       7081 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
       7141 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
       7201 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
       7261 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
       7321 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
       7381 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
       7441 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
       7501 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
       7561 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
       7621 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
       7681 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
       7741 ggggacccag agagggccta tttcccatga ttccttcata tttgcatata cgatacaagg
       7801 ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta gtacaaaata
       7861 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
       7921 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
       7981 tGTGGAAAGG ACGAAACACC gGAGACGGTT GTAAATGAGC ACACAAAATA CACATGCTAA
       8041 AATATTATAT TCTATGACCT TTATAAAATC AACCAAAATC TTCTTTTTAA TAACTTTAGT
       8101 ATCAATAATT AGAATTTTTA TGTTCCTTTT TGCAAACTTT TAATAAAAAT GAGCAAAATA
       8161 AAAAAACGCT AGTTTTAGTA ACTCGCGTTG TTTTCTTCAC CTTTAATAAT AGCTACTCCA
       8221 CCACTTGTTC CTAAGCGGTC AGCTCCTGCT TCAATCATTT TTTGAGCATC TTCAAATGTT
       8281 CTAACTCCAC CAGCTGCTTT AACTAAAGCA TTGTCTTTAA CAACTGACTT CATTAGTTTA
       8341 ACATCTTCAA ATGTTGCACC TGATTTTGAA AATCCTGTTG ATGTTTTAAC AAATTCTAAT
       8401 CCAGCTTCAA CAGCTATTTC ACAAGCTTTC ATGATTTCTT CTTTTGTTAA TAAACAATTT
       8461 TCCATAATAC ATTTAACAAC ATGTGATCCA GCTGCTTTTT TTACAGCTTT CATGTCTTCT
       8521 AAAACTAATT CATAATTTTT GTCTTTTAAT GCACCAATAT TTAATACCAT ATCAATTTCT
       8581 GTTGCACCAT CTTTAATTGC TTCAGAAACT TCGAATGCTT TTGTAGCTGT TGTGCATGCA
       8641 CCTAGAGGAA AACCTACAAC ATTTGTTATT CCTACATTTG TGCCTTTTAA TAATTCTTTA
       8701 CAATAGCTTG TTCAATATGA ATTAACACAA ACTGTTGCAA AATCAAATTC AATTGCTTCA
       8761 TCACATAATT GTTTAATTTC AGCTTTCGTA GCATCTTGTT TTAATAATGT GTGATCTATA
       8821 TATTTGTTTA GTTTCATTTT TTCTCCTATA TATTCATTTT TAATTTTAAT TCTTTAATAA
       8881 TTTCGTCTAC TTTAACTTTA GCGTTTTGAA CAGATTCACC AACACCTATA AAATAAATTT
       8941 TTAGTTTAGG TTCAGTTCCA CTTGGGCGAA CAGCAAATCA TGACTTATCT TCTAAATAAA
       9001 ATTTTAGTAA GTCTTGTCCT GGCATATTAT ACATTCCATC GATGTAGTCT TCAACATTAA
       9061 CAACTTTAAG TCCAGCAATT TGAGTTAAGG GTGTTGCTCT CAATGATTTC ATTAATGGTT
       9121 CAATTTTTAA TTTCTTTTCT TCTGGTTTAA AATTCAAGTT TAAAGTGAAA GTGTAATATG
       9181 CACCCATTTC TTTAAATAAA TCTTCTAAAT AGTCTACTAA TGTTTTATTT TGTTTTTTAT
       9241 AAAATCAAGC AGCCTCTGCT ATTAATATAG AAGCTTGTAT TCCATCTTTA TCTCTAGCTG
       9301 AGTCATCAAT TACATATCCA TAACTTTCTT CATAAGCAAA AACAAAATTT AATCCGTTAT
       9361 CTTCTTCTTT AGCAATTTCT CTACCCATTC ATTTAAATCC AGTTAAAGTT TTTACAATAT
       9421 TAACTCCATA TTTTTCATGA GCGATTCTAT CACCCAAATC ACTTGTTACA AAACTTGAAT
       9481 ATAGAGCCGG ATTTTTTGGA ATGCTATTTA AGCGTTTTAG ATTTGATAAT TTTCAATCAA
       9541 TTAAAATTGG TCCTGTTTGA TTTCCATCTA ATCTTACAAA ATGACCATCA TGTTTTATTG
       9601 CCATTCCAAA TCTGTCAGCA TCTGGGTCAT TCATAATAAT AATATCTGCA TCATGTTTAA
       9661 TACCATATTC AAGCGGTATT TTTCATGCAG GATCAAATTC TGGATTTGGA TTTACAACAT
       9721 TTTTAAATGT TTCATCTTCA AATGCATGCT CTTCAACCTC AATAACGTTA TATCCTGATT
       9781 CACGTAATAT TTTTGGGGTA AATTTAGTTC CTGTTCCATT AACTGCGCTA AAAATAATTT
       9841 TTAAATCTTT TTTAGCTTCT TGCTCTTTTT TGTACGTCTC Tgttttagag ctaGAAAtag
       9901 caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcTTT
       9961 TTTaagcttg gcgtaactag atcttgagac aaatggcagt attcatccac aattttaaaa
      10021 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
      10081 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
      10141 acagggacag cagagatcca ctttggcgcc ggctcgaggg gg
//

