LOCUS pBS_SK_mCherryRO 5613 bp ds-DNA circular 19-FEB-2014 DEFINITION pBluescript SK(+) vector DNA, phagemid excised from lambda ZAP. ACCESSION X52325 VERSION X52325.1 GI:58067 DBSOURCE GenBank KEYWORDS artificial sequence; cloning vector; expression vector; vector. SOURCE synthetic construct (unknown) ORGANISM synthetic construct synthetic construct other sequences; artificial sequences REFERENCE 1 AUTHORS Short,J.M., Fernandez,J.M., Sorge,J.A. and Huse,W.D. TITLE Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties JOURNAL Nucleic Acids Res. 16 (15), 7583-7600 (1988) MEDLINE 88319944 REFERENCE 2 AUTHORS Alting-Mees,M.A. and Short,J.M. TITLE pBluescript II: gene mapping vectors JOURNAL Nucleic Acids Res. 17 (22), 9494 (1989) MEDLINE 90067967 REFERENCE 3 AUTHORS Thomas,E.A. TITLE Direct Submission JOURNAL Submitted (20-FEB-1990) Thomas E.A., Stratagene Clonin Systems, 11099 North Torney Pines Rd., La Jolla, CA 92037, USA COMMENT COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 728..3421 /label=Sequenced /ApEinfo_fwdcolor=#a8ffb8 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin complement(135..441) /note="f1(+) origin" /label=f1(+) origin /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature join(1867..3084,3086..3352) /note="CLC name: bsr R cassette" /label=CLC name: bsr R cassette /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0609 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS complement(join(460..697,3353..3468)) /note="beta-gal" /label=beta-gal /ApEinfo_fwdcolor=#5a0077 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 1955..2377 /label=bsr cds /ApEinfo_fwdcolor=#5a0077 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 1069..1776 /label=mCherry /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 promoter 702..1052 /label=CMV promoter /ApEinfo_fwdcolor=#00bfbf /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1783..1789 /label=Esp3I /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature join(2542..3084,3086..3352) /label=SV40 /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0609 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 polyA_signal complement(3197..3352) /label=SV40 late polyA /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 polyA_signal 3289..3339 /label=SV40 polyA signal /ApEinfo_fwdcolor=#eab001 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1867..1887 /label=New Feature /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3333..3352 /label=New Feature(1) /ApEinfo_label=New Feature /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1854..1860 /label=Esp3I(1) /ApEinfo_label=Esp3I /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1790..1793 /label=New Feature(2) /ApEinfo_label=New Feature /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1850..1853 /label=New Feature(3) /ApEinfo_label=New Feature /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 promoter complement(3469..3590) /note="lac promoter" /label=lac promoter /ApEinfo_fwdcolor=#00bfbf /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin complement(3810..4477) /note="pUC origin" /label=pUC origin /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS complement(4628..5485) /note="ampicillin resistance" /note="ampr" /label=ampicillin resistance /ApEinfo_fwdcolor=#5a0077 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 CTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC 61 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA 121 GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC 181 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC 241 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG 301 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA 361 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC 421 CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCC CATTCGCCAT TCAGGCTGCG 481 CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG 541 GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG 601 TAAAACGACG GCCAGTGAGC GCGCGTAATA CGACTCACTA TAGGGCGAAT TGGGTACCGG 661 GCCCCCCCTC GAGGTCGACG GTATCGATAA GCTTGATTAT GCCAAGTACG CCCCCTATTG 721 ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC TTATGGGACT 781 TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG ATGCGGTTTT 841 GGCAGTACAT CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC 901 CCATTGACGT CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC 961 GTAACAACTC CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA 1021 TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCG GATTCACCAT GGTGAGCAAG 1081 GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT GCACATGGAG 1141 GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG CCCCTACGAG 1201 GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCTGCCCTT CGCCTGGGAC 1261 ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG TGAAGCACCC CGCCGACATC 1321 CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT GATGAACTTC 1381 GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG CGAGTTCATC 1441 TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT GCAGAAGAAG 1501 ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG AGGACGGCGC CCTGAAGGGC 1561 GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCTGA GGTCAAGACC 1621 ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA CATCAAGTTG 1681 GACATCACCT CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG CGCCGAGGGC 1741 CGCCACTCCA CCGGCGGCAT GGACGAGCTG TACAAGaagc ttcgtctcTG TCGgataaTC 1801 TGCAACTCCA GTCTttccag aagatgggcG GGAGTCTTCT GGGCAaagaT CTGcgagacg 1861 GAATTCTGCT GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTAATT CAAGCTTGGA 1921 TTCATTTTTG CAGAAATCGG AGGAAGAAGA ATATATGAAA ACATTTAACA TTTCTCAACA 1981 AGATCTAGAA TTAGTAGAAG TAGCGACAGA GAAGATTACA ATGCTTTATG AGGATAATAA 2041 ACATCATGTG GGAGCGGCAA TTCGTACGAA AACAGGAGAA ATCATTTCGG CAGTACATAT 2101 TGAAGCGTAT ATAGGACGAG TAACTGTTTG TGCAGAAGCC ATTGCGATTG GTAGTGCAGT 2161 TTCGAATGGA CAAAAGGATT TTGACACGAT TGTAGCTGTT AGACACCCTT ATTCTGACGA 2221 AGTAGATAGA AGTATTCGAG TGGTAAGTCC TTGTGGTATG TGTAGGGAGT TGATTTCAGA 2281 CTATGCACCA GATTGTTTTG TGTTAATAGA AATGAATGGC AAGTTAGTCA AAACTACGAT 2341 TGAAGAACTC ATTCCACTCA AATATACCCG AAATTAAAAG TTTTACCATA CCAAGCTTGG 2401 CTGCTGCCTG AGGCTGGACG ACCTCGCGGA GTTCTACCGG CAGTGCAAAT CCGTCGGCAT 2461 CCAGGAAACC AGCAGCGGCT ATCCGCGCAT CCATGCCCCC GAACTGCAGG AGTGGGGAGG 2521 CACGATGGCC GCTTTGGTCC GGATCTTTGT GAAGGAACCT TACTTCTGTG GTGTGACATA 2581 ATTGGACAAA CTACCTACAG AGATTTAAAG CTCTAAGGTA AATATAAAAT TTTTAAGTGT 2641 ATAATGTGTT AAACTACTGA TTCTAATTGT TTGTGTATTT TAGATTCCAA CCTATGGAAC 2701 TGATGAATGG GAGCAGTGGT GGAATGCCTT TAATGAGGAA AACCTGTTTT GCTCAGAAGA 2761 AATGCCATCT AGTGATGATG AGGCTACTGC TGACTCTCAA CATTCTACTC CTCCAAAAAA 2821 GAAGAGAAAG GTAGAAGACC CCAAGGACTT TCCTTCAGAA TTGCTAAGTT TTTTGAGTCA 2881 TGCTGTGTTT AGTAATAGAA CTCTTGCTTG CTTTGCTATT TACACCACAA AGGAAAAAGC 2941 TGCACTGCTA TACAAGAAAA TTATGGAAAA ATATTCTGTA ACCTTTATAA GTAGGCATAA 3001 CAGTTATAAT CATAACATAC TGTTTTTTCT TACTCCACAC AGGCATAGAG TGTCTGCTAT 3061 TAATAACTAT GCTCAAAAAT TGTGTACCTT TAGCTTTTTA ATTTGTAAAG GGGTTAATAA 3121 GGAATATTTG ATGTATAGTG CCTTGACTAG AGATCATAAT CAGCCATACC ACATTTGTAG 3181 AGGTTTTACT TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA 3241 ATGCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA 3301 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGGAATTCCT 3361 GCAGCCCGGG GGATCCACTA GTTCTAGAGC GGCCGCCACC GCGGTGGAGC TCCAGCTTTT 3421 GTTCCCTTTA GTGAGGGTTA ATTGCGCGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG 3481 TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA 3541 AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG 3601 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA 3661 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG 3721 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 3781 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 3841 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 3901 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 3961 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 4021 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC 4081 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 4141 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 4201 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 4261 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA 4321 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 4381 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA 4441 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG 4501 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 4561 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG 4621 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT 4681 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG 4741 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA 4801 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA 4861 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC 4921 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT 4981 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA 5041 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT 5101 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT 5161 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA 5221 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG 5281 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA 5341 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA 5401 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG 5461 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 5521 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 5581 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CAC //