LOCUS       pEM791_map   7800 bp    DNA   circular             6-SEP-2011
DEFINITION  vector.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|585234243|
COMMENT     VNTDBDATE|600522955|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEM791_map@3|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     misc_feature    complement(9807..9905)
                     /dnas_title="Bla promoter"
                     /vntifkey="21"
                     /label=Bla promoter
     promoter        complement(4354..5296)
                     /dnas_title="UBC promoter"
                     /vntifkey="29"
                     /label=UBC promoter
     misc_feature    7007..7537
                     /dnas_title="globin 3'UTR"
                     /vntifkey="21"
                     /label=globin 3'UTR
     misc_feature    7560..7593
                     /dnas_title="LoxP"
                     /vntifkey="21"
                     /label=LoxP
     misc_feature    complement(8946..9806)
                     /dnas_title="Bla (AmpR)"
                     /vntifkey="21"
                     /label=Bla (AmpR)
     misc_feature    5319..5586
                     /dnas_title="TRE (Tet promoter)"
                     /vntifkey="21"
                     /label=TRE (Tet promoter)
     misc_feature    1..1
                     /dnas_title="TKpA"
                     /vntifkey="21"
                     /label=TKpA
     misc_feature    5742..5768
                     /dnas_title="miR-155-5'arm"
                     /vntifkey="21"
                     /label=miR-155-5'arm
     misc_feature    5790..5835
                     /dnas_title="miR-155_3'arm"
                     /vntifkey="21"
                     /label=miR-155_3'arm
     misc_feature    5644..5927
                     /dnas_title="recombinant intron"
                     /vntifkey="21"
                     /label=recombinant intron
     misc_feature    8187..8720
                     /dnas_title="ORI"
                     /vntifkey="21"
                     /label=ORI
     misc_feature    312..345
                     /dnas_title="Lox2271"
                     /vntifkey="21"
                     /label=Lox2271
     misc_feature    2907..3101
                     /dnas_title="bGH_3'UTR"
                     /vntifkey="21"
                     /label=bGH_3'UTR
     misc_feature    complement(3109..3366)
                     /dnas_title="SV40_3'UTR"
                     /vntifkey="21"
                     /label=SV40_3'UTR
     misc_feature    complement(3367..4074)
                     /dnas_title="rtTA3"
                     /vntifkey="21"
                     /label=rtTA3
     PCR_primer      3376..3397
                     /note="primer 209"
     PCR_primer      complement(3460..3482)
                     /note="primer 210"
     misc_feature    2..311
                     /dnas_title="TKpA"
                     /vntifkey="21"
                     /label=TKpA
     promoter        complement(4079..4353)
                     /dnas_title="UBC promoter"
                     /vntifkey="29"
                     /label=UBC promoter
     misc_feature    1599..2183
                     /note="IRES"
     PCR_primer      1520..1545
                     /note="primer 239"
     misc_feature    2187..2784
                     /dnas_title="Pur"
                     /vntifkey="21"
                     /label=Pur
     PCR_primer      2184..2206
                     /note="primer 174"
     PCR_primer      complement(2163..2183)
                     /note="primer 168"
     PCR_primer      complement(2765..2786)
                     /note="primer 212"
     gene            5964..6671
                     /dnas_title="mCherry"
                     /gene="mCherry"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
                     NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
                     ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
                     YERAEGRHSTGGMDELYK"
                     /note=""
     gene            6705..6992
                     /dnas_title="FRB T2098L"
                     /label=FRB T2098L
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQT
                     LKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISKTR"
                     /gene="FRB T2098L"
     gene            6672..6704
                     /label="MCS/linker peptide"
                     /translation="GTAGPGSTGSR"
                     /note="linker region connecting YFP to FRB, contains
                     multiple cloning site"
     misc_feature    501..527
                     /dnas_title="miR-155-5'arm"
                     /vntifkey="21"
                     /label=miR-155-5'arm
     misc_feature    403..724
                     /dnas_title="recombinant intron"
                     /vntifkey="21"
                     /label=recombinant intron
     misc_feature    588..632
                     /dnas_title="miR-155_3'arm"
                     /vntifkey="21"
                     /label=miR-155_3'arm
     misc_feature    794..1510
                     /dnas_title="EGFP"
                     /vntifkey="21"
                     /label=EGFP
     PCR_primer      352..391
                     /note="primer 240"
     PCR_primer      complement(1487..1519)
                     /note="primer 241"
     misc_feature    5742..5835
                     /note="empty miRNA"
     misc_feature    528..587
                     /note="FKBP12 shRNA"
                     /note="this shRNA sequence targets the 3' UTR of FKBP12
                     in human cells"
     source          1..9932
                     /dnas_title="pEM791_map@3"
ORIGIN      
        1 aagcttaata aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
       61 aaagacagaa taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
      121 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
      181 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
      241 gtcggggcgg caggccctgc catagccact ggccccgtgg gttagggacg gggtccccca
      301 ttcgagaata aataacttcg tataaagtat cctatacgaa gttatcaccc tgcagtacgt
      361 atccggtagc cttgcagaag ttggtcgtga ggcactgggc aggtaagtat caaggttaca
      421 agacaggttt aaggagacca atagaaactg ggcttgtcga gacagagaag actcttgcgt
      481 ttcggtcgac ttctagagct ctggaggctt gctgaaggct gtatgctGAT ATGGATTCAT
      541 GTGCACATGG TTTTGGCCAC TGACTGACCA TGTGCATGAA TCCATATcag gacacaaggc
      601 ctgttactag cactcacatg gaacaaatgg ccgaattcgg cgctagctgc tgatatcgca
      661 tacgcgtgga ccagataggc acctattggt cttactgaca tccactttgc ctttctctcc
      721 acaggtgtcg gtaccgcggg cccgggatcc accGGCGCGC CCTGCAGGCG GCCGCCTCGA
      781 Gccggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
      841 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
      901 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
      961 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     1021 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     1081 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     1141 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     1201 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     1261 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     1321 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     1381 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     1441 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     1501 gctgtacaag tccggataac cggaggtgca ggctgcctat gcatgttaac aattgcatcg
     1561 ataagcttga tatcgaattc ctgcagcccg ggggatccgc ccctctccct cccccccccc
     1621 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     1681 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     1741 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     1801 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     1861 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     1921 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     1981 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     2041 ggtaccccat tgtatgggat ctgatctggg gcctcggtac acatgcttta catgtgttta
     2101 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
     2161 aacacgatga taatatggcc acaaccatga ccgagtacaa gcccacggtg cgcctcgcca
     2221 cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     2281 ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     2341 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     2401 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     2461 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     2521 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtgtcgc
     2581 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     2641 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     2701 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     2761 gcatgacccg caagcccggt gcctAGccgg agaccgaaag gagcgcacga ccccatggct
     2821 ccgaccgaag ccgacccggg cggccccgcc gaccccgcac ccgcccccga ggcccaccga
     2881 ctctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     2941 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     3001 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     3061 ggggcaggac agcaaggggg aggattggga agacaatagc actagtttcg cgttaagata
     3121 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     3181 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     3241 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     3301 caagtaaaac ctctacaaat gtggtatggc tgattatgat cagttatcta gatccggtgg
     3361 atctatttac ccggggagca tgtcaaggtc aaaatcgtca agggcatcgg ctgggagcat
     3421 gtctaagtca aaatcgtcaa gggcgtcggt cggcccgccg ctttcgcact ttagctgttt
     3481 ctccaggcca catatgatta gttccaggcc gaaaaggaag gcaggttcgg ctccctgccg
     3541 gtcgaacagc tcaattgctt gtctcagaag tgggggcata gaatcggtgg taggtgtctc
     3601 tctttcctct tttgctactt gatgctcctg ttcctccaat acgcagccca gtgtaaagtg
     3661 gcccacggcg gacagagcgt acagtgcgtt ctccagggag aagccttgct gacacaggaa
     3721 cgcgagctga ttttccaggg tttcgtactg tttctctgtt gggcgggtgc cgagatgcac
     3781 tttagccccg tcgcgatgtg agaggagagc acagcggtat gacttggcgt tgttccgcag
     3841 aaagtcttgc catgactcgc cttccagggg gcagaagtgg gtatgatgcc tgtccagcat
     3901 ctcgattggc agggcatcga gcagggcccg cttgttcttc acgtgccagt acagggtagg
     3961 ctgctcaact cccagctttt gagcgagttt ccttgtcgtc aggccttcga taccgacacc
     4021 attgagtaat tccagagctc cgtttatgac tttgctcttg tccagcctag acatggtggc
     4081 tagcgtctaa caaaaaagcc aaaaacggcc agaatttagc ggacaattta ctagtctaac
     4141 actgaaaatt acatattgac ccaaatgatt acatttcaaa aggtgcctaa aaaacttcac
     4201 aaaacacact cgccaacccc gagcgcatag ttcaaaaccg gagcttcagc tacttaagaa
     4261 gataggtaca taaaaccgac caaagaaact gacgcctcac ttatccctcc cctcaccaga
     4321 ggtccggcgc ctgtcgattc aggagagcct accctaggcc cgaaccctgc gtcctgcgac
     4381 ggagaaaagc ctaccgcaca cctaccggca ggtggcccca ccctgcatta taagccaaca
     4441 gaacgggtga cgtcacgaca cgacgagggc gcgcgctccc aaaggtacgg gtgcactgcc
     4501 caacggcacc gccataactg ccgcccccgc aacagacgac aaaccgagtt ctccagtcag
     4561 tgacaaactt cacgtcaggg tccccagatg gtgccccagc ccatctcacc cgaataagag
     4621 ctttcccgca ttagcgaagg cctcaagacc ttgggttctt gccgcccacc atgcccccca
     4681 ccttgtttca acgacctcac agcccgcctc acaagcgtct tccattcaag actcgggaac
     4741 agccgccatt ttgctgcgct ccccccaacc cccagttcag ggcaaccttg ctcgcggacc
     4801 cagactacag cccttggcgg tctctccaca cgcttccgtc ccaccgagcg gcccggcggc
     4861 cacgaaagcc ccggccagcc cagcagcccg ctactcacca agtgacgatc acagcgatcc
     4921 acaaacaaga accgcgaccc aaatcccggc tgcgacggaa ctagctgtgc cacacccggc
     4981 gcgtccttat ataatcatcg gcgttcaccg ccccacggag atccctccgc agaatcgccg
     5041 agaagggact acttttcctc gcctgttccg ctctctggaa agaaaaccag tgccctagag
     5101 tcacccaagt cccgtcctaa aatgtccttc tgctgatact ggggttctaa ggccgagtct
     5161 tatgagcagc gggccgctgt cctgagcgtc cgggcggaag gatcaggacg ctcgctgcgc
     5221 ccttcgtctg acgtggcagc gctcgccgtg aggagggggg cgcccgcggg aggcgccaaa
     5281 acccggcgcg gaggccacgc gtactagacg agtttactcc ctatcagtga tagagaacga
     5341 tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
     5401 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttat
     5461 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat
     5521 gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
     5581 agatcgcacc gggtgactct cttaaggtag ccttgcagaa gttggtcgtg aggcactggg
     5641 caggtaagta tcaaggttac aagacaggtt taaggagacc aatagaaact gggcttgtcg
     5701 agacagagaa gactcttgcg tttcggtcga cttctagagc tctggaggct tgctgaaggc
     5761 tgtatgctag agacgtacag atgcgtctca caggacacaa ggcctgttac tagcactcac
     5821 atggaacaaa tggccgaatt cggcgctagc tgctgatatc gcatacgcgt ggaccagata
     5881 ggcacctatt ggtcttactg acatccactt tgcctttctc tccacaggtg tcggtaccgc
     5941 gggcccggga tccaccggTC ACCatggtga gcaagggcga ggaggataac atggccatca
     6001 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
     6061 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     6121 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
     6181 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
     6241 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
     6301 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
     6361 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg
     6421 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
     6481 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
     6541 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
     6601 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
     6661 agctgtacaa gGGTACCGCG GGCCCGGGAT CCACCGGATC TAGAATCCTC TGGCATGAGA
     6721 TGTGGCATGA AGGCCTGGAA GAGGCATCTC GTTTGTACTT TGGGGAAAGG AACGTGAAAG
     6781 GCATGTTTGA GGTGCTGGAG CCCTTGCATG CTATGATGGA ACGGGGCCCC CAGACTCTGA
     6841 AGGAAACATC CTTTAATCAG GCCTATGGTC GAGATTTAAT GGAGGCCCAA GAGTGGTGCA
     6901 GGAAGTACAT GAAATCAGGG AATGTCAAGG ACCTCCTCCA AGCCTGGGAC CTCTATTATC
     6961 ATGTGTTCCG ACGAATCTCA AAGACTAGAT AAgtacaagt aaacactcag gtgcaggctg
     7021 cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc
     7081 tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg
     7141 gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac
     7201 tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga
     7261 gtttggcaac atatgccata tgctggctgc catgaacaaa ggtggctata aagaggtcat
     7321 cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga
     7381 ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt
     7441 tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct
     7501 gtccctcttc tcttatgaag atccctcgac ctgagcgtcg agctagcata tggatccata
     7561 taacttcgta taatgtatgc tatacgaagt tattacgata ccgtcaactt gtttattgca
     7621 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     7681 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaggt acccagcttt
     7741 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct
     7801 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7861 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     7921 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7981 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8041 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8101 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8161 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8221 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8281 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8341 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8401 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8461 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8521 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8581 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     8641 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8701 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8761 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8821 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8881 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8941 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     9001 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9061 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9121 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9181 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9241 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9301 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9361 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9421 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9481 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9541 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9601 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9661 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9721 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9781 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9841 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9901 ggggttccgc gcacatttcc ccgaaaagtg cc
//

