LOCUS       Exported               13743 bp ds-DNA     circular SYN 18-MAY-2015
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    58284-attachment
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 18, 2015 from SnapGene 2.7.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13743
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          8611..10790
                     /organism="Homo sapiens"
                     /chromosome="7"
                     /map="7p22"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
     misc_feature    join(1..7566,10888..13743)
     misc_feature    join(1..7566,7884..8589,10796..13743)
     misc_feature    join(1..8589,10796..13743)
     misc_feature    1..309
                     /note="TKpA"
     misc_feature    1
                     /note="TKpA"
     misc_feature    312..345
                     /note="Lox2271"
     intron          403..724
                     /note="recombinant intron"
     misc_feature    501..527
                     /note="miR-155-5'arm"
     misc_feature    588..632
                     /note="miR-155_3'arm"
     CDS             765..3116
                     /codon_start=1
                     /gene="Mis12-GFP-FKBP fusion ORF"
                     /note="Mis12-GFP-FKBP fusion ORF"
                     /translation="MSVDPMTYEAQFFGFTPQTCMLRIYIAFQDYLFEVMQAVEQVILK
                     KLDGIPDCDISPVQIRKCTEKFLCFMKGHFDNLFSKMEQLFLQLILRIPSNILLPEDKC
                     KETPYSEEDFQHLQKEIEQLQEKYKTELCTKQALLAELEEQKIVQAKLKQTLTFFDELH
                     NVGRDHGTSDFRESLVSLVQNSRKLQNIRDNVEKESKRLKISMLAVATMVSKGEELFTG
                     VVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQ
                     CFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGI
                     DFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTP
                     IGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGEFSRGV
                     QVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEE
                     GVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLETRGVQVETISPGD
                     GRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQ
                     RAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLETSEFGVQVETISPGDGRTFPKR
                     GQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTIS
                     PDYAYGATGHPGIIPPHATLVFDVELLKLE"
     misc_feature    765..1379
                     /note="hMis12"
     gene            1398..2114
                     /gene="EGFP"
                     /note="EGFP"
     CDS             2127..2453
                     /codon_start=1
                     /note="FKBP"
                     /translation="SRGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRN
                     KPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVE
                     LLKLE"
     CDS             2457..2780
                     /codon_start=1
                     /note="FKBP*"
                     /note="FKBP wt amino acid sequence with silent mutations, 
                     not same set of mutations as Fv' in pC4-Fv2E"
                     /translation="RGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     gene            2793..3116
                     /gene="FKBP"
                     /note="FKBP domain"
                     /note=""
     misc_feature    3222..3806
                     /note="IRES"
     misc_feature    3810..4407
                     /note="Pur"
     misc_feature    4530..4724
                     /note="bGH_3'UTR"
     misc_feature    complement(4732..4989)
                     /note="SV40_3'UTR"
     misc_feature    complement(4990..5697)
                     /note="rtTA3"
     promoter        complement(5702..5976)
                     /note="UBC promoter"
     promoter        complement(5977..6919)
                     /note="UBC promoter"
     misc_feature    6942..7209
                     /note="TRE (Tet promoter)"
     misc_feature    7267..7550
                     /note="recombinant intron"
     misc_feature    7365..7391
                     /note="miR-155-5'arm"
     misc_feature    7413..7458
                     /note="miR-155_3'arm"
     misc_feature    7567..7883
     gene            7599..7880
                     /gene="FRB T2098L"
                     /note="FRB T2098L"
     misc_feature    join(7884..8589,10796..10887)
     misc_feature    7896..8589
                     /note="mCherry"
     misc_feature    8590..10795
     misc_feature    8611..10791
     gene            8611..10790
                     /gene="MAD1L1"
                     /gene_synonym="MAD1; PIG9; TP53I9; TXBP181"
                     /note="MAD1L1"
                     /note="MAD1 mitotic arrest deficient-like 1 (yeast)"
                     /db_xref="GeneID:8379"
                     /db_xref="HGNC:6762"
                     /db_xref="HPRD:04065"
                     /db_xref="MIM:602686"
     CDS             8611..10790
                     /codon_start=1
                     /gene="MAD1L1"
                     /gene_synonym="MAD1; PIG9; TP53I9; TXBP181"
                     /product="mitotic spindle assembly checkpoint protein MAD1"
                     /note="MAD1L1"
                     /note="mitotic-arrest deficient 1, yeast, homolog-like 1; 
                     tumor protein p53 inducible protein 9; MAD1-like protein 1;
                     tax-binding protein 181; mitotic checkpoint MAD1 protein 
                     homolog; mitotic arrest deficient 1-like protein 1"
                     /db_xref="GI:62243374"
                     /db_xref="CCDS:CCDS43539.1"
                     /db_xref="GeneID:8379"
                     /db_xref="HGNC:6762"
                     /db_xref="HPRD:04065"
                     /db_xref="MIM:602686"
                     /protein_id="NP_001013859.1"
                     /translation="MWKTWGKTPWFYPP*DL*TTSSLSVWREALDWIFLPRPQVLCRCS
                     TSRACSWRKEQSRSVRSPTSSRWSGRKCRWS*VTRGLEWSWREQPAPVPGTTSVRSTAT
                     RSS*RASGSFRSGRPGRRRRCRSSWSATGSVSRTWMLPARGCVRKRTVWPRLARPSTH*
                     RGGSRNCSGA*WTRRCG*SAWSRRSRSCRSSWTCNTKNARKPIRKSRNSRPAKKQEQTT
                     SSRLRIWSRSCPCKSRMQRL*RT*SLSWYGSLGWNGS*SSCGRRARTCGRRARTCGR*E
                     RPTGCSRKSWKGCRGSWGARRRCRRRWLAWSWRTRGCWPSCKAGRDWTRPWA*ASGLQK
                     TFPDSWLSCSRGSLP*RTRTAPSPAAPGGWRRPGSSCRRSSGRSAASCWRRGRSARPTR
                     RWPGGSRNGSCCSPRSGTVCGPSWGPTTAS*PRPSTHPS*RGACGRLRIWCRRCTATAP
                     RWRLSCRRPWRSWEARNKEQTCWRWS*RC*SLSPALPNRASCSPGRRRTRSG*RSRSWK
                     ASGVGWRRKRGCWRHSWSGELCRVTMTRAGPKCCT*A*TPPVWPGSACTRTTASCRRSA
                     SDCAGSCAPWREEAPSQPTLRLPPRVCHRPRRWQS*RSRWRVPS*RTSGSRRFSRPRSR
                     SSARPATRSPATRSTSPRRTSTG*PRCTPSTQATASSSRPPAPRVPRCSYWRQSSHTPW
                     ASSSRCTCGARTASLPSSARSPSSSSAARPWR"
     exon            8611..8762
                     /gene="MAD1L1"
                     /gene_synonym="MAD1; PIG9; TP53I9; TXBP181"
                     /number="3"
                     /note="exon 3"
                     /inference="alignment:same species"
     misc_feature    8658..8660
                     /gene="MAD1L1"
                     /gene_synonym="MAD1; PIG9; TP53I9; TXBP181"
                     /note="Phosphoserine; propagated from UniProtKB/Swiss-Prot 
                     (Q9Y6D9.2); phosphorylation site"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_feature    8735..8756
                     /note="primer 26"
     exon            8763..8903
                     /gene="MAD1L1"
                     /gene_synonym="MAD1; PIG9; TP53I9; TXBP181"
                     /number="4"
                     /note="exon 4"
                     /inference="alignment:same species"
     misc_feature    8793..8795
                     /gene="MAD1L1"
                     /gene_synonym="MAD1; PIG9; TP53I9; TXBP181"
                     /note="N6-acetyllysine; propagated from 
                     UniProtKB/Swiss-Prot (Q9Y6D9.2); acetylation site"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     exon            8904..9083
                     /gene="MAD1L1"
                     /gene_synonym="MAD1; PIG9; TP53I9; TXBP181"
                     /number="5"
                     /note="exon 5"
                     /inference="alignment:same species"
     exon            9084..9208
                     /gene="MAD1L1"
                     /gene_synonym="MAD1; PIG9; TP53I9; TXBP181"
                     /number="6"
                     /note="exon 6"
                     /inference="alignment:same species"
     exon            9209..9290
                     /gene="MAD1L1"
                     /gene_synonym="MAD1; PIG9; TP53I9; TXBP181"
                     /number="7"
                     /note="exon 7"
                     /inference="alignment:same species"
     misc_feature    9252..9254
                     /gene="MAD1L1"
                     /gene_synonym="MAD1; PIG9; TP53I9; TXBP181"
                     /note="Phosphoserine; propagated from UniProtKB/Swiss-Prot 
                     (Q9Y6D9.2); phosphorylation site"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     exon            9291..9442
                     /gene="MAD1L1"
                     /gene_synonym="MAD1; PIG9; TP53I9; TXBP181"
                     /number="8"
                     /note="exon 8"
                     /inference="alignment:same species"
     misc_feature    9300..9321
                     /note="primer 32"
     repeat_region   9396..9437
                     /note="tandem repeat created by 21 base insertion"
     misc_feature    9396..9416
                     /note="21 base insertion in sequencing data"
     exon            9443..9557
                     /gene="MAD1L1"
                     /gene_synonym="MAD1; PIG9; TP53I9; TXBP181"
                     /number="9"
                     /note="exon 9"
                     /inference="alignment:same species"
     exon            9558..9619
                     /gene="MAD1L1"
                     /gene_synonym="MAD1; PIG9; TP53I9; TXBP181"
                     /number="10"
                     /note="exon 10"
                     /inference="alignment:same species"
     exon            9620..9706
                     /gene="MAD1L1"
                     /gene_synonym="MAD1; PIG9; TP53I9; TXBP181"
                     /number="11"
                     /note="exon 11"
                     /inference="alignment:same species"
     misc_feature    9672..9693
                     /note="primer 30"
     exon            9707..9851
                     /gene="MAD1L1"
                     /gene_synonym="MAD1; PIG9; TP53I9; TXBP181"
                     /number="12"
                     /note="exon 12"
                     /inference="alignment:same species"
     misc_feature    9771..10229
                     /gene="MAD1L1"
                     /gene_synonym="MAD1; PIG9; TP53I9; TXBP181"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Y6D9.2); 
                     Region: Necessary for interaction with NEK2"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     exon            9852..9992
                     /gene="MAD1L1"
                     /gene_synonym="MAD1; PIG9; TP53I9; TXBP181"
                     /number="13"
                     /note="exon 13"
                     /inference="alignment:same species"
     misc_feature    9915..9917
                     /gene="MAD1L1"
                     /gene_synonym="MAD1; PIG9; TP53I9; TXBP181"
                     /note="Phosphoserine; propagated from UniProtKB/Swiss-Prot 
                     (Q9Y6D9.2); phosphorylation site"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     exon            9993..10049
                     /gene="MAD1L1"
                     /gene_synonym="MAD1; PIG9; TP53I9; TXBP181"
                     /number="14"
                     /note="exon 14"
                     /inference="alignment:same species"
     exon            10050..10138
                     /gene="MAD1L1"
                     /gene_synonym="MAD1; PIG9; TP53I9; TXBP181"
                     /number="15"
                     /note="exon 15"
                     /inference="alignment:same species"
     exon            10139..10229
                     /gene="MAD1L1"
                     /gene_synonym="MAD1; PIG9; TP53I9; TXBP181"
                     /number="16"
                     /note="exon 16"
                     /inference="alignment:same species"
     exon            10230..10440
                     /gene="MAD1L1"
                     /gene_synonym="MAD1; PIG9; TP53I9; TXBP181"
                     /number="17"
                     /note="exon 17"
                     /inference="alignment:same species"
     misc_feature    10306
                     /note="R558H dbSNP: rs1801368 found in a human cancer cell 
                     line"
     exon            10441..10631
                     /gene="MAD1L1"
                     /gene_synonym="MAD1; PIG9; TP53I9; TXBP181"
                     /number="18"
                     /note="exon 18"
                     /inference="alignment:same species"
     misc_feature    10556..10575
                     /note="primer 28"
     exon            10632..10790
                     /gene="MAD1L1"
                     /gene_synonym="MAD1; PIG9; TP53I9; TXBP181"
                     /number="19"
                     /note="exon 19"
                     /inference="alignment:same species"
     misc_feature    10796..10802
                     /note="mCherry C-terminal extension"
     misc_feature    10818..10887
                     /note="globin 3'UTR"
     misc_feature    10888..11348
                     /note="globin 3'UTR"
     misc_feature    11371..11404
                     /note="LoxP"
     misc_feature    11998..12531
                     /note="ORI"
     misc_feature    complement(12757..13617)
                     /note="Bla (AmpR)"
     misc_feature    complement(13618..13716)
                     /note="Bla promoter"
ORIGIN
        1 aagcttaata aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
       61 aaagacagaa taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
      121 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
      181 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
      241 gtcggggcgg caggccctgc catagccact ggccccgtgg gttagggacg gggtccccca
      301 ttcgagaata aataacttcg tataaagtat cctatacgaa gttatcaccc tgcagtacgt
      361 atccggtagc cttgcagaag ttggtcgtga ggcactgggc aggtaagtat caaggttaca
      421 agacaggttt aaggagacca atagaaactg ggcttgtcga gacagagaag actcttgcgt
      481 ttcggtcgac ttctagagct ctggaggctt gctgaaggct gtatgctgat atggattcat
      541 gtgcacatgg ttttggccac tgactgacca tgtgcatgaa tccatatcag gacacaaggc
      601 ctgttactag cactcacatg gaacaaatgg ccgaattcgg cgctagctgc tgatatcgca
      661 tacgcgtgga ccagataggc acctattggt cttactgaca tccactttgc ctttctctcc
      721 acaggtgtcg gtaccgcggg cccgggatcc accggcgcgc caccatgtct gtggatccaa
      781 tgacctacga ggcccagttc tttggcttca cgccacaaac gtgcatgctt cggatctaca
      841 ttgcatttca agactaccta tttgaagtga tgcaggccgt tgaacaggtt attctgaaga
      901 agctggatgg catcccagac tgtgacatta gcccagtgca gattcgcaaa tgcacagaga
      961 agtttctttg cttcatgaaa ggacattttg ataacctttt tagcaaaatg gagcaactgt
     1021 ttttgcagct gattttacgt attccctcaa acatcttgct tcctgaagat aaatgtaagg
     1081 agacacctta tagtgaggaa gattttcagc atctccagaa agaaattgaa cagttacagg
     1141 agaagtacaa gactgaatta tgtactaagc aggcccttct tgcagaatta gaagagcaaa
     1201 aaattgttca ggccaaactc aaacagacgt tgactttctt tgatgagctt cataatgttg
     1261 gcagagatca tgggactagt gattttaggg agagtttagt atccctggtt cagaactcca
     1321 gaaaactaca gaacattaga gacaatgtgg aaaaggaatc gaaacgactg aaaatatcta
     1381 tgctagcagt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     1441 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     1501 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     1561 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     1621 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     1681 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     1741 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     1801 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     1861 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     1921 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     1981 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     2041 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     2101 acgagctgta caagggtggt gaattctcta gaggagtgca ggtggaaacc atctccccag
     2161 gagacgggcg caccttcccc aagcgcggcc agacctgcgt ggtgcactac accgggatgc
     2221 ttgaagatgg aaagaaattt gattcctccc gggacagaaa caagcccttt aagtttatgc
     2281 taggcaagca ggaggtgatc cgaggctggg aagaaggggt tgcccagatg agtgtgggtc
     2341 agagagccaa actgactata tctccagatt atgcctatgg tgccactggg cacccaggca
     2401 tcatcccacc acatgccact ctcgtcttcg atgtggagct tctaaaactg gaaactagag
     2461 gcgtgcaggt cgagaccatc agccccggcg acggccgcac ctttcccaag agaggccaga
     2521 cttgcgtggt ccactacacc ggcatgctgg aggacggcaa gaagttcgac agcagccgcg
     2581 accgcaacaa gcccttcaag ttcatgctgg gcaaacagga agtgatccgc ggctgggagg
     2641 aaggcgtggc tcagatgagc gtggggcagc gggccaagct gaccatcagc cccgactatg
     2701 cctacggcgc caccggccac cccggcatca tcccccccca cgccaccctg gtgttcgacg
     2761 tggagctgct gaagctggag actagtgaat tcggagtgca ggtggaaacc atctccccag
     2821 gagacgggcg caccttcccc aagcgcggcc agacctgcgt ggtgcactac accgggatgc
     2881 ttgaagatgg aaagaaattt gattcctccc gggacagaaa caagcccttt aagtttatgc
     2941 taggcaagca ggaggtgatc cgaggctggg aagaaggggt tgcccagatg agtgtgggtc
     3001 agagagccaa actgactata tctccagatt atgcctatgg tgccactggg cacccaggca
     3061 tcatcccacc acatgccact ctcgtcttcg atgtggagct tctaaaactg gaatgatcta
     3121 gagggcccta ttctccggat aaccggaggt gcaggctgcc tatgcatgtt aacaattgca
     3181 tcgataagct tgatatcgaa ttcctgcagc ccgggggatc cgcccctctc cctccccccc
     3241 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
     3301 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
     3361 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
     3421 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
     3481 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
     3541 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
     3601 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
     3661 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tacacatgct ttacatgtgt
     3721 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     3781 aaaaacacga tgataatatg gccacaacca tgaccgagta caagcccacg gtgcgcctcg
     3841 ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc
     3901 ccgccacgcg ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag
     3961 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     4021 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     4081 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     4141 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtgt
     4201 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     4261 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     4321 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     4381 ggtgcatgac ccgcaagccc ggtgcctagc cggagaccga aaggagcgca cgaccccatg
     4441 gctccgaccg aagccgaccc gggcggcccc gccgaccccg cacccgcccc cgaggcccac
     4501 cgactctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     4561 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     4621 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     4681 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcactagtt tcgcgttaag
     4741 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     4801 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     4861 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     4921 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcagttat ctagatccgg
     4981 tggatctatt tacccgggga gcatgtcaag gtcaaaatcg tcaagggcat cggctgggag
     5041 catgtctaag tcaaaatcgt caagggcgtc ggtcggcccg ccgctttcgc actttagctg
     5101 tttctccagg ccacatatga ttagttccag gccgaaaagg aaggcaggtt cggctccctg
     5161 ccggtcgaac agctcaattg cttgtctcag aagtgggggc atagaatcgg tggtaggtgt
     5221 ctctctttcc tcttttgcta cttgatgctc ctgttcctcc aatacgcagc ccagtgtaaa
     5281 gtggcccacg gcggacagag cgtacagtgc gttctccagg gagaagcctt gctgacacag
     5341 gaacgcgagc tgattttcca gggtttcgta ctgtttctct gttgggcggg tgccgagatg
     5401 cactttagcc ccgtcgcgat gtgagaggag agcacagcgg tatgacttgg cgttgttccg
     5461 cagaaagtct tgccatgact cgccttccag ggggcagaag tgggtatgat gcctgtccag
     5521 catctcgatt ggcagggcat cgagcagggc ccgcttgttc ttcacgtgcc agtacagggt
     5581 aggctgctca actcccagct tttgagcgag tttccttgtc gtcaggcctt cgataccgac
     5641 accattgagt aattccagag ctccgtttat gactttgctc ttgtccagcc tagacatggt
     5701 ggctagcgtc taacaaaaaa gccaaaaacg gccagaattt agcggacaat ttactagtct
     5761 aacactgaaa attacatatt gacccaaatg attacatttc aaaaggtgcc taaaaaactt
     5821 cacaaaacac actcgccaac cccgagcgca tagttcaaaa ccggagcttc agctacttaa
     5881 gaagataggt acataaaacc gaccaaagaa actgacgcct cacttatccc tcccctcacc
     5941 agaggtccgg cgcctgtcga ttcaggagag cctaccctag gcccgaaccc tgcgtcctgc
     6001 gacggagaaa agcctaccgc acacctaccg gcaggtggcc ccaccctgca ttataagcca
     6061 acagaacggg tgacgtcacg acacgacgag ggcgcgcgct cccaaaggta cgggtgcact
     6121 gcccaacggc accgccataa ctgccgcccc cgcaacagac gacaaaccga gttctccagt
     6181 cagtgacaaa cttcacgtca gggtccccag atggtgcccc agcccatctc acccgaataa
     6241 gagctttccc gcattagcga aggcctcaag accttgggtt cttgccgccc accatgcccc
     6301 ccaccttgtt tcaacgacct cacagcccgc ctcacaagcg tcttccattc aagactcggg
     6361 aacagccgcc attttgctgc gctcccccca acccccagtt cagggcaacc ttgctcgcgg
     6421 acccagacta cagcccttgg cggtctctcc acacgcttcc gtcccaccga gcggcccggc
     6481 ggccacgaaa gccccggcca gcccagcagc ccgctactca ccaagtgacg atcacagcga
     6541 tccacaaaca agaaccgcga cccaaatccc ggctgcgacg gaactagctg tgccacaccc
     6601 ggcgcgtcct tatataatca tcggcgttca ccgccccacg gagatccctc cgcagaatcg
     6661 ccgagaaggg actacttttc ctcgcctgtt ccgctctctg gaaagaaaac cagtgcccta
     6721 gagtcaccca agtcccgtcc taaaatgtcc ttctgctgat actggggttc taaggccgag
     6781 tcttatgagc agcgggccgc tgtcctgagc gtccgggcgg aaggatcagg acgctcgctg
     6841 cgcccttcgt ctgacgtggc agcgctcgcc gtgaggaggg gggcgcccgc gggaggcgcc
     6901 aaaacccggc gcggaggcca cgcgtactag acgagtttac tccctatcag tgatagagaa
     6961 cgatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca
     7021 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt
     7081 tatccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg
     7141 tatgtcgagg taggcgtgta cggtgggagg cctatataag cagagctcgt ttagtgaacc
     7201 gtcagatcgc accgggtgac tctcttaagg tagccttgca gaagttggtc gtgaggcact
     7261 gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa actgggcttg
     7321 tcgagacaga gaagactctt gcgtttcggt cgacttctag agctctggag gcttgctgaa
     7381 ggctgtatgc tagagacgta cagatgcgtc tcacaggaca caaggcctgt tactagcact
     7441 cacatggaac aaatggccga attcggcgct agctgctgat atcgcatacg cgtggaccag
     7501 ataggcacct attggtctta ctgacatcca ctttgccttt ctctccacag gtgtcggtac
     7561 cgcgggcccg ggatccaccg gtcaccatgg cttctagaat cctctggcat gagatgtggc
     7621 atgaaggcct ggaagaggca tctcgtttgt actttgggga aaggaacgtg aaaggcatgt
     7681 ttgaggtgct ggagcccttg catgctatga tggaacgggg cccccagact ctgaaggaaa
     7741 catcctttaa tcaggcctat ggtcgagatt taatggaggc ccaagagtgg tgcaggaagt
     7801 acatgaaatc agggaatgtc aaggacctcc tccaagcctg ggacctctat tatcatgtgt
     7861 tccgacgaat ctcaaagact agtccggtcg ccaccatggt gagcaagggc gaggaggata
     7921 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     7981 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     8041 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     8101 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     8161 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     8221 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     8281 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     8341 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     8401 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     8461 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     8521 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     8581 gcggcatgga cgagctgtat aaggccagcc tgatggaaga cctgggggaa aacaccatgg
     8641 ttttatccac cctgagatct ttgaacaact tcatctctca gcgtgtggag ggaggctctg
     8701 gactggatat ttctacctcg gccccaggtt ctctgcagat gcagtaccag cagagcatgc
     8761 agctggagga aagagcagag cagatccgtt cgaagtccca cctcatccag gtggagcggg
     8821 agaaaatgca gatggagctg agtcacaaga gggctcgagt ggagctggag agagcagcca
     8881 gcaccagtgc caggaactac gagcgtgagg tcgaccgcaa ccaggagctc ctgacgcgca
     8941 tccggcagct tcaggagcgg gaggccgggg cggaggagaa gatgcaggag cagctggagc
     9001 gcaacaggca gtgtcagcag aacttggatg ctgccagcaa gaggctgcgt gagaaagagg
     9061 acagtctggc ccaggctggc gagaccatca acgcactgaa ggggaggatc tcggaactgc
     9121 agtggagcgt gatggaccag gagatgcggg tgaagcgcct ggagtcggag aagcaggagc
     9181 tgcaggagca gctggacctg caacacaaaa aatgccagga agccaatcag aaaatccagg
     9241 aactccaggc cagccaagaa gcaagagcag accacgagca gcagattaag gatctggagc
     9301 agaagctgtc cctgcaagag caggatgcag cgattgtgaa gaacatgaag tctgagctgg
     9361 tacggctccc taggctggaa cgggagctga agcagctgcg ggaggagagc gcgcacctgc
     9421 gggaggagag cgcgcacctg cgggagatga gagagaccaa cgggctgctc caggaagagc
     9481 tggaagggct gcagaggaag ctggggcgcc aggagaagat gcaggagacg ctggttggct
     9541 tggagctgga gaacgagagg ctgctggcca agctgcaaag ctgggagaga ctggaccaga
     9601 ccatgggcct gagcatcagg actccagaag acctttccag attcgtggtt gagctgcagc
     9661 agagggagct tgccttgaag gacaagaaca gcgccgtcac cagcagcgcc cgggggctgg
     9721 agaaggccag gcagcagctg caggaggagc tccggcaggt cagcggccag ctgttggagg
     9781 agaggaagaa gcgcgagacc cacgaggcgc tggcccggag gctccagaaa cgggtcctgc
     9841 tgctcaccaa ggagcgggac ggtatgcggg ccatcctggg gtcctacgac agcgagctga
     9901 ccccggccga gtactcaccc cagctgacgc ggcgcatgcg ggaggctgag gatatggtgc
     9961 agaaggtgca cagccacagc gccgagatgg aggctcagct gtcgcaggcc ctggaggagc
    10021 tgggaggcca gaaacaaaga gcagacatgc tggagatgga gctgaagatg ctgaagtctc
    10081 agtccagctc tgccgaacag agcttcctgt tctccaggga ggaggcggac acgctcaggt
    10141 tgaaggtcga ggagctggaa ggcgagcgga gtcggctgga ggaggaaaag aggatgctgg
    10201 aggcacagct ggagcggcga gctctgcagg gtgactatga ccagagcagg accaaagtgc
    10261 tgcacatgag cctgaacccc accagtgtgg ccaggcagcg cctgcacgag gaccacagcc
    10321 agctgcaggc ggagtgcgag cgactgcgcg ggctcctgcg cgccatggag agaggaggca
    10381 ccgtcccagc cgaccttgag gctgccgccg cgagtctgcc atcgtccaag gaggtggcag
    10441 agctgaagaa gcaggtggag agtgccgagc tgaagaacca gcggctcaag gaggttttcc
    10501 agaccaagat ccaggagttc cgcaaggcct gctacacgct caccggctac cagatcgaca
    10561 tcaccacgga gaaccagtac cggctgacct cgctgtacgc cgagcaccca ggcgactgcc
    10621 tcatcttcaa ggccaccagc ccctcgggtt ccaagatgca gctactggag acagagttct
    10681 cacacaccgt gggcgagctc atcgaggtgc acctgcggcg ccaggacagc atccctgcct
    10741 tcctcagctc gctcaccctc gagctcttca gccgccagac cgtggcgtag ggatccctat
    10801 gatgtacaag taaacactca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc
    10861 aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac
    10921 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca
    10981 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat
    11041 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccat atgctggctg
    11101 ccatgaacaa aggtggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca
    11161 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt
    11221 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt
    11281 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgaa gatccctcga
    11341 cctgagcgtc gagctagcat atggatccat ataacttcgt ataatgtatg ctatacgaag
    11401 ttattacgat accgtcaact tgtttattgc agcttataat ggttacaaat aaagcaatag
    11461 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
    11521 actcatcaat gtatcttagg tacccagctt ttgttccctt tagtgagggt taattgcgcg
    11581 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
    11641 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
    11701 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
    11761 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
    11821 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
    11881 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
    11941 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
    12001 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
    12061 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
    12121 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
    12181 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
    12241 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
    12301 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    12361 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    12421 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
    12481 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
    12541 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    12601 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    12661 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    12721 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
    12781 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    12841 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
    12901 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
    12961 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    13021 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    13081 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    13141 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    13201 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    13261 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    13321 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    13381 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    13441 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    13501 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    13561 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    13621 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    13681 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    13741 gcc
//
