LOCUS       FRB-mCH-Mad1mini       11863 bp    DNA     circular     24-NOV-2014
FEATURES             Location/Qualifiers
     misc_feature    1..1
                     /dnas_title="TKpA"
                     /vntifkey="21"
                     /label=TKpA
     misc_feature    312..345
                     /dnas_title="Lox2271"
                     /vntifkey="21"
                     /label=Lox2271
     misc_feature    1..309
                     /dnas_title="TKpA"
                     /vntifkey="21"
                     /label=TKpA
     intron          403..724
                     /dnas_title="recombinant intron"
                     /vntifkey="21"
                     /label=recombinant intron
     misc_feature    501..527
                     /dnas_title="miR-155-5'arm"
                     /vntifkey="21"
                     /label=miR-155-5'arm
     misc_feature    588..632
                     /dnas_title="miR-155_3'arm"
                     /vntifkey="21"
                     /label=miR-155_3'arm
     CDS             765..3116
                     /dnas_title="Mis12-GFP-FKBP fusion ORF"
                     /gene="Mis12-GFP-FKBP fusion ORF"
                     /translation="MSVDPMTYEAQFFGFTPQTCMLRIYIAFQDYLFEVMQAVEQVIL
                     KKLDGIPDCDISPVQIRKCTEKFLCFMKGHFDNLFSKMEQLFLQLILRIPSNILLPED
                     KCKETPYSEEDFQHLQKEIEQLQEKYKTELCTKQALLAELEEQKIVQAKLKQTLTFFD
                     ELHNVGRDHGTSDFRESLVSLVQNSRKLQNIRDNVEKESKRLKISMLAVATMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTL
                     TYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNR
                     IELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLAD
                     HYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK
                     GGEFSRGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGK
                     QEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLETRG
                     VQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGW
                     EEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLETSEFGVQVET
                     ISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVA
                     QMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLE"
     misc_feature    765..1379
                     /note="hMis12"
     gene            1398..2114
                     /dnas_title="EGFP"
                     /gene="EGFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK"
     CDS             2127..2453
                     /label=FKBP
     CDS             2457..2780
                     /label=FKBP*
                     /note="FKBP wt amino acid sequence with silent mutations,
                     not same set of mutations as Fv' in pC4-Fv2E"
     gene            2793..3116
                     /dnas_title="FKBP"
                     /gene="FKBP"
                     /label=FKBP domain
                     /note=""
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVE
                     LLKLE"
     misc_feature    3222..3806
                     /note="IRES"
     misc_feature    3810..4407
                     /dnas_title="Pur"
                     /vntifkey="21"
                     /label=Pur
     misc_feature    4530..4724
                     /dnas_title="bGH_3'UTR"
                     /vntifkey="21"
                     /label=bGH_3'UTR
     misc_feature    complement(4732..4989)
                     /dnas_title="SV40_3'UTR"
                     /vntifkey="21"
                     /label=SV40_3'UTR
     misc_feature    complement(4990..5697)
                     /dnas_title="rtTA3"
                     /vntifkey="21"
                     /label=rtTA3
     promoter        complement(5702..6919)
                     /dnas_title="UBC promoter"
                     /vntifkey="29"
                     /label=UBC promoter
     misc_feature    6942..7209
                     /dnas_title="TRE (Tet promoter)"
                     /vntifkey="21"
                     /label=TRE (Tet promoter)
     misc_feature    7365..7391
                     /dnas_title="miR-155-5'arm"
                     /vntifkey="21"
                     /label=miR-155-5'arm
     misc_feature    7413..7458
                     /dnas_title="miR-155_3'arm"
                     /vntifkey="21"
                     /label=miR-155_3'arm
     misc_feature    7267..7550
                     /dnas_title="recombinant intron"
                     /vntifkey="21"
                     /label=recombinant intron
     misc_feature    8938..9468
                     /dnas_title="globin 3'UTR"
                     /vntifkey="21"
                     /label=globin 3'UTR
     misc_feature    9491..9524
                     /dnas_title="LoxP"
                     /vntifkey="21"
                     /label=LoxP
     misc_feature    10118..10651
                     /dnas_title="ORI"
                     /vntifkey="21"
                     /label=ORI
     misc_feature    complement(10877..11737)
                     /dnas_title="Bla (AmpR)"
                     /vntifkey="21"
                     /label=Bla (AmpR)
     misc_feature    complement(11738..11836)
                     /dnas_title="Bla promoter"
                     /vntifkey="21"
                     /label=Bla promoter
     gene            7599..7880
                     /dnas_title="FRB T2098L"
                     /label=FRB T2098L
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQT
                     LKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISKTR"
                     /gene="FRB T2098L"
     misc_feature    7896..8589
                     /note="mCherry"
     misc_feature    8916..8922
                     /note="mCherry C-terminal extension"
     gene            8610..8912
                     /dnas_title="MAD1L1"
                     /gene="MAD1L1"
                     /gene_synonym="MAD1; PIG9; TP53I9; TXBP181"
                     /note="MAD1 mitotic arrest deficient-like 1 (yeast)"
                     /db_xref="GeneID:8379"
                     /db_xref="HGNC:6762"
                     /db_xref="HPRD:04065"
                     /db_xref="MIM:602686"
                     /translation=".WKTWGKTPWFYPP.DL.TTSSLSVWREALDWIFLPRPQVLCRC
                     STSRACSWRKEQSRSVRSPTSSRWSGRKCRWS.VTRGLEWSWREQPAPVPGTTSVRST
                     ATRSS.RASGSFRSGRPGRRRRCRSSWSATGSVSRTWMLPARGCVRKRTVWPRLARPS
                     TH.RGGSRNCSGA.WTRRCG.SAWSRRSRSCRSSWTCNTKNARKPIRKSRNSRPAKKQ
                     EQTTSSRLRIWSRSCPCKSRMQRL.RT.SLSWYGSLGWNGS.SSCGRRARTCGRRART
                     CGR.ERPTGCSRKSWKGCRGSWGARRRCRRRWLAWSWRTRGCWPSCKAGRDWTRPWA.
                     ASGLQKTFPDSWLSCSRGSLP.RTRTAPSPAAPGGWRRPGSSCRRSSGRSAASCWRRG
                     RSARPTRRWPGGSRNGSCCSPRSGTVCGPSWGPTTAS.PRPSTHPS.RGACGRLRIWC
                     RRCTATAPRWRLSCRRPWRSWEARNKEQTCWRWS.RC.SLSPALPNRASCSPGRRRTR
                     SG.RSRSWKASGVGWRRKRGCWRHSWSGELCRVTMTRAGPKCCT.A.TPPVWPGSACT
                     RTTASCRRSASDCAGSCAPWREEAPSQPTLRLPPRVCHRPRRWQS.RSRWRVPS.RTS
                     GSRRFSRPRSRSSARPATRSPATRSTSPRRTSTG.PRCTPSTQATASSSRPPAPRVPR
                     CSYWRQSSHTPWASSSRCTCGARTASLPSSARSPSSSSAARPWR"
     misc_feature    528..587
                     /note="FKBP12 shRNA"
     misc_feature    7392..7412
                     /note="BsmBI stuffer"
     source          1..11863
                     /dnas_title="FRB-mCH-Mad1minimal"
ORIGIN      
        1 aagcttaata aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
       61 aaagacagaa taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
      121 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
      181 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
      241 gtcggggcgg caggccctgc catagccact ggccccgtgg gttagggacg gggtccccca
      301 ttcgagaata aataacttcg tataaagtat cctatacgaa gttatcaccc tgcagtacgt
      361 atccggtagc cttgcagaag ttggtcgtga ggcactgggc aggtaagtat caaggttaca
      421 agacaggttt aaggagacca atagaaactg ggcttgtcga gacagagaag actcttgcgt
      481 ttcggtcgac ttctagagct ctggaggctt gctgaaggct gtatgctGAT ATGGATTCAT
      541 GTGCACATGG TTTTGGCCAC TGACTGACCA TGTGCATGAA TCCATATcag gacacaaggc
      601 ctgttactag cactcacatg gaacaaatgg ccgaattcgg cgctagctgc tgatatcgca
      661 tacgcgtgga ccagataggc acctattggt cttactgaca tccactttgc ctttctctcc
      721 acaggtgtcg gtaccgcggg cccgggatcc accGGcgcgc caccatgtct gtggatccaa
      781 tgacctacga ggcccagttc tttggcttca cgccacaaac gtgcatgctt cggatctaca
      841 ttgcatttca agactaccta tttgaagtga tgcaggccgt tgaacaggtt attctgaaga
      901 agctggatgg catcccagac tgtgacatta gcccagtgca gattcgcaaa tgcacagaga
      961 agtttctttg cttcatgaaa ggacattttg ataacctttt tagcaaaatg gagcaactgt
     1021 ttttgcagct gattttacgt attccctcaa acatcttgct tcctgaagat aaatgtaagg
     1081 agacacctta tagtgaggaa gattttcagc atctccagaa agaaattgaa cagttacagg
     1141 agaagtacaa gactgaatta tgtactaagc aggcccttct tgcagaatta gaagagcaaa
     1201 aaattgttca ggccaaactc aaacagacgt tgactttctt tgatgagctt cataatgttg
     1261 gcagagatca tgggactagt gattttaggg agagtttagt atccctggtt cagaactcca
     1321 gaaaactaca gaacattaga gacaatgtgg aaaaggaatc gaaacgactg aaaatatcta
     1381 tgctagcAGT CGCCACCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA
     1441 TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG
     1501 AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC
     1561 CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT
     1621 ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC
     1681 AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT
     1741 TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG
     1801 GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG
     1861 CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG
     1921 GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC
     1981 TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA
     2041 AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG
     2101 ACGAGCTGTA CAAGGGTGGT gAATTCtcta gaggagtgca ggtggaaacc atctccccag
     2161 gagacgggcg caccttcccc aagcgcggcc agacctgcgt ggtgcactac accgggatgc
     2221 ttgaagatgg aaagaaattt gattcctccc gggacagaaa caagcccttt aagtttatgc
     2281 taggcaagca ggaggtgatc cgaggctggg aagaaggggt tgcccagatg agtgtgggtc
     2341 agagagccaa actgactata tctccagatt atgcctatgg tgccactggg cacccaggca
     2401 tcatcccacc acatgccact ctcgtcttcg atgtggagct tctaaaactg gaaactagag
     2461 gcgtgcaggt cgagaccatc agccccggcg acggccgcac ctttcccaag agaggccaga
     2521 cttgcgtggt ccactacacc ggcatgctgg aggacggcaa gaagttcgac agcagccgcg
     2581 accgcaacaa gcccttcaag ttcatgctgg gcaaacagga agtgatccgc ggctgggagg
     2641 aaggcgtggc tcagatgagc gtggggcagc gggccaagct gaccatcagc cccgactatg
     2701 cctacggcgc caccggccac cccggcatca tcccccccca cgccaccctg gtgttcgacg
     2761 tggagctgct gaagctggag actagtGaat tcGGAGTGCA GGTGGAAACC ATCTCCCCAG
     2821 GAGACGGGCG CACCTTCCCC AAGCGCGGCC AGACCTGCGT GGTGCACTAC ACCGGGATGC
     2881 TTGAAGATGG AAAGAAATTT GATTCCTCCC GGGACAGAAA CAAGCCCTTT AAGTTTATGC
     2941 TAGGCAAGCA GGAGGTGATC CGAGGCTGGG AAGAAGGGGT TGCCCAGATG AGTGTGGGTC
     3001 AGAGAGCCAA ACTGACTATA TCTCCAGATT ATGCCTATGG TGCCACTGGG CACCCAGGCA
     3061 TCATCCCACC ACATGCCACT CTCGTCTTCG ATGTGGAGCT TCTAAAACTG GAATGAtcta
     3121 gagggcccta ttctccggat aaccggaggt gcaggctgcc tatgcatgtt aacaattgca
     3181 tcgataagct tgatatcgaa ttcctgcagc ccgggggatc cgcccctctc cctccccccc
     3241 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
     3301 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
     3361 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
     3421 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
     3481 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
     3541 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
     3601 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
     3661 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tacacatgct ttacatgtgt
     3721 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     3781 aaaaacacga tgataatatg gccacaacca tgaccgagta caagcccacg gtgcgcctcg
     3841 ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc
     3901 ccgccacgcg ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag
     3961 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     4021 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     4081 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     4141 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtgt
     4201 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     4261 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     4321 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     4381 ggtgcatgac ccgcaagccc ggtgcctAGc cggagaccga aaggagcgca cgaccccatg
     4441 gctccgaccg aagccgaccc gggcggcccc gccgaccccg cacccgcccc cgaggcccac
     4501 cgactctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     4561 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     4621 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     4681 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcactagtt tcgcgttaag
     4741 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     4801 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     4861 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     4921 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcagttat ctagatccgg
     4981 tggatctatt tacccgggga gcatgtcaag gtcaaaatcg tcaagggcat cggctgggag
     5041 catgtctaag tcaaaatcgt caagggcgtc ggtcggcccg ccgctttcgc actttagctg
     5101 tttctccagg ccacatatga ttagttccag gccgaaaagg aaggcaggtt cggctccctg
     5161 ccggtcgaac agctcaattg cttgtctcag aagtgggggc atagaatcgg tggtaggtgt
     5221 ctctctttcc tcttttgcta cttgatgctc ctgttcctcc aatacgcagc ccagtgtaaa
     5281 gtggcccacg gcggacagag cgtacagtgc gttctccagg gagaagcctt gctgacacag
     5341 gaacgcgagc tgattttcca gggtttcgta ctgtttctct gttgggcggg tgccgagatg
     5401 cactttagcc ccgtcgcgat gtgagaggag agcacagcgg tatgacttgg cgttgttccg
     5461 cagaaagtct tgccatgact cgccttccag ggggcagaag tgggtatgat gcctgtccag
     5521 catctcgatt ggcagggcat cgagcagggc ccgcttgttc ttcacgtgcc agtacagggt
     5581 aggctgctca actcccagct tttgagcgag tttccttgtc gtcaggcctt cgataccgac
     5641 accattgagt aattccagag ctccgtttat gactttgctc ttgtccagcc tagacatggt
     5701 ggctagcgtc taacaaaaaa gccaaaaacg gccagaattt agcggacaat ttactagtct
     5761 aacactgaaa attacatatt gacccaaatg attacatttc aaaaggtgcc taaaaaactt
     5821 cacaaaacac actcgccaac cccgagcgca tagttcaaaa ccggagcttc agctacttaa
     5881 gaagataggt acataaaacc gaccaaagaa actgacgcct cacttatccc tcccctcacc
     5941 agaggtccgg cgcctgtcga ttcaggagag cctaccctag gcccgaaccc tgcgtcctgc
     6001 gacggagaaa agcctaccgc acacctaccg gcaggtggcc ccaccctgca ttataagcca
     6061 acagaacggg tgacgtcacg acacgacgag ggcgcgcgct cccaaaggta cgggtgcact
     6121 gcccaacggc accgccataa ctgccgcccc cgcaacagac gacaaaccga gttctccagt
     6181 cagtgacaaa cttcacgtca gggtccccag atggtgcccc agcccatctc acccgaataa
     6241 gagctttccc gcattagcga aggcctcaag accttgggtt cttgccgccc accatgcccc
     6301 ccaccttgtt tcaacgacct cacagcccgc ctcacaagcg tcttccattc aagactcggg
     6361 aacagccgcc attttgctgc gctcccccca acccccagtt cagggcaacc ttgctcgcgg
     6421 acccagacta cagcccttgg cggtctctcc acacgcttcc gtcccaccga gcggcccggc
     6481 ggccacgaaa gccccggcca gcccagcagc ccgctactca ccaagtgacg atcacagcga
     6541 tccacaaaca agaaccgcga cccaaatccc ggctgcgacg gaactagctg tgccacaccc
     6601 ggcgcgtcct tatataatca tcggcgttca ccgccccacg gagatccctc cgcagaatcg
     6661 ccgagaaggg actacttttc ctcgcctgtt ccgctctctg gaaagaaaac cagtgcccta
     6721 gagtcaccca agtcccgtcc taaaatgtcc ttctgctgat actggggttc taaggccgag
     6781 tcttatgagc agcgggccgc tgtcctgagc gtccgggcgg aaggatcagg acgctcgctg
     6841 cgcccttcgt ctgacgtggc agcgctcgcc gtgaggaggg gggcgcccgc gggaggcgcc
     6901 aaaacccggc gcggaggcca cgcgtactag acgagtttac tccctatcag tgatagagaa
     6961 cgatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca
     7021 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt
     7081 tatccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg
     7141 tatgtcgagg taggcgtgta cggtgggagg cctatataag cagagctcgt ttagtgaacc
     7201 gtcagatcgc accgggtgac tctcttaagg tagccttgca gaagttggtc gtgaggcact
     7261 gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa actgggcttg
     7321 tcgagacaga gaagactctt gcgtttcggt cgacttctag agctctggag gcttgctgaa
     7381 ggctgtatgc tagagacgta cagatgcgtc tcacaggaca caaggcctgt tactagcact
     7441 cacatggaac aaatggccga attcggcgct agctgctgat atcgcatacg cgtggaccag
     7501 ataggcacct attggtctta ctgacatcca ctttgccttt ctctccacag gtgtcggtac
     7561 cgcgggcccg ggatccaccg gTCACCATGG CTTCTAGAAT CCTCTGGCAT GAGATGTGGC
     7621 ATGAAGGCCT GGAAGAGGCA TCTCGTTTGT ACTTTGGGGA AAGGAACGTG AAAGGCATGT
     7681 TTGAGGTGCT GGAGCCCTTG CATGCTATGA TGGAACGGGG CCCCCAGACT CTGAAGGAAA
     7741 CATCCTTTAA TCAGGCCTAT GGTCGAGATT TAATGGAGGC CCAAGAGTGG TGCAGGAAGT
     7801 ACATGAAATC AGGGAATGTC AAGGACCTCC TCCAAGCCTG GGACCTCTAT TATCATGTGT
     7861 TCCGACGAAT CTCAAAGACT AGTccggtcg ccaccATGGT GAGCAAGGGC GAGGAGGATA
     7921 ACATGGCCAT CATCAAGGAG TTCATGCGCT TCAAGGTGCA CATGGAGGGC TCCGTGAACG
     7981 GCCACGAGTT CGAGATCGAG GGCGAGGGCG AGGGCCGCCC CTACGAGGGC ACCCAGACCG
     8041 CCAAGCTGAA GGTGACCAAG GGTGGCCCCC TGCCCTTCGC CTGGGACATC CTGTCCCCTC
     8101 AGTTCATGTA CGGCTCCAAG GCCTACGTGA AGCACCCCGC CGACATCCCC GACTACTTGA
     8161 AGCTGTCCTT CCCCGAGGGC TTCAAGTGGG AGCGCGTGAT GAACTTCGAG GACGGCGGCG
     8221 TGGTGACCGT GACCCAGGAC TCCTCCCTGC AGGACGGCGA GTTCATCTAC AAGGTGAAGC
     8281 TGCGCGGCAC CAACTTCCCC TCCGACGGCC CCGTAATGCA GAAGAAGACC ATGGGCTGGG
     8341 AGGCCTCCTC CGAGCGGATG TACCCCGAGG ACGGCGCCCT GAAGGGCGAG ATCAAGCAGA
     8401 GGCTGAAGCT GAAGGACGGC GGCCACTACG ACGCTGAGGT CAAGACCACC TACAAGGCCA
     8461 AGAAGCCCGT GCAGCTGCCC GGCGCCTACA ACGTCAACAT CAAGTTGGAC ATCACctccc
     8521 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     8581 gcggcatgga cgagctgtat aaGGCCGGCt ccagctctgc cgaacagagc ttcctgttct
     8641 ccagggagga ggcggacacg ctcaggttga aggtcgagga gctggaaggc gagcggagtc
     8701 ggctggagga ggaaaagagg atgctggagg cacagctgga gcggcgagct ctgcagggtg
     8761 actatgacca gagcaggacc aaagtgctgc acatgagcct gaaccccacc agtgtggcca
     8821 ggcagcgcct gcAcgaggac cacagccagc tgcaggcgga gtgcgagcga ctgcgcgggc
     8881 tcctgcgcgc catggagaga ggaggcaccg tctgaCCTAT GATgtacaag taaacactca
     8941 ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata
     9001 ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca
     9061 tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     9121 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta
     9181 tttggtttag agtttggcaa catatgccat atgctggctg ccatgaacaa aggtggctat
     9241 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag
     9301 ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat
     9361 ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc
     9421 cagtcatagc tgtccctctt ctcttatgaa gatccctcga cctgagcgtc gagctagcat
     9481 atggatccat ataacttcgt ataatgtatg ctatacgaag ttattacgat accgtcaact
     9541 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     9601 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttagg
     9661 tacccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat
     9721 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     9781 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     9841 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     9901 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     9961 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
    10021 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
    10081 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
    10141 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    10201 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    10261 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    10321 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    10381 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    10441 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    10501 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
    10561 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    10621 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    10681 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    10741 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    10801 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    10861 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    10921 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    10981 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    11041 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    11101 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    11161 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    11221 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    11281 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    11341 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    11401 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    11461 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    11521 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    11581 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    11641 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    11701 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    11761 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    11821 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gcc
//

