LOCUS       pRS416	4898 bp	DNA	SYN	12-Sep-2011
DEFINITION  pRS416
ACCESSION    
KEYWORDS    -
SOURCE      -
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          join(complement(<5019..>5061),complement(<7730..7928),
                     complement(1..>2832))
                     /organism="pRS416"
                     /note="mol_type=other DNA [Split]"
     misc_feature    7878..7900
                     /label="pGEX_3_primer"
     misc_feature    complement(5049..>5061)
                     /label="pBluescriptKS_primer"
     misc_feature    2812..2828
                     /label="pBluescriptSK_primer"
     promoter        2757..2776
                     /label="T3_promoter"
     misc_feature    2722..2740
                     /label="M13_reverse_primer"
     misc_feature    2701..2723
                     /label="M13_pUC_rev_primer"
     rep_origin      1730..2349
                     /label="pBR322_origin"
     CDS             715..1575
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /note="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPV..."
     gene            715..1575
                     /label="Ampicillin"
                     /gene="Ampicillin"
     promoter        645..673
                     /label="AmpR_promoter"
     misc_feature    complement(65..583)
                     /label="CEN6_ARS4"
     frag            join(5062..>6309,<6313..7724)
                     /note="5232 to 7894 of pAG41hygro-GPD-ccdB [Split]"
     CDS             complement(<6313..7338)
                     /gene="hph"
                     /product="hygromycin B phosphotransferase"
     rep_origin      5539..5845
                     /label="f1_origin"
     terminator      complement(6070..6309)
                     /gene="TEF-TER"
                     /product="TEF-TER"
     promoter        complement(5333..5351)
                     /label="T7_promoter"
     misc_feature    5370..5513
                     /label="lacZ_a"
     misc_feature    complement(5361..5377)
                     /label="M13_forward20_primer"
     misc_feature    complement(5370..5392)
                     /label="M13_pUC_fwd_primer"
     misc_feature    complement(<5062..5065)
                     /label="pBluescriptKS_primer [Split]"
     terminator      5074..5313
                     /label="CYC1_terminator"
     misc_feature    complement(5083..5101)
                     /label="CYC1_primer"
     misc_feature    complement(5083..5101)
                     /label="pYESTrp_rev_primer"
     promoter        complement(7339..7719)
                     /gene="TEF-PRO"
                     /product="TEF-PRO"
     source          join(<5062..>6309,<6313..>7724)
                     /note="dnas_title=pAG41hygro-GPD-ccdB [Split]"
     misc_feature    6030..6035
                     /note="/locus_tag=Fusion point, old Nhe1-Spe1 cut site "
     frag            2833..5018
                     /note="2833 to 5018 of p41Neo 3-frag GW ccDB 1"
     frag            complement(2833..5018)
                     /note="1 to 2186 of Untitled 9"
     frag            2833..5018
                     /note="2067 to 4252 of pRS416 3-frag GW P4-P3r ccDB"
     frag            2833..5018
                     /note="21 to 2206 of pDEST R4-R3 Vector II"
     primer_bind     2833..2852
                     /note="/label=R4-F N primer"
     primer_bind     4997..5018
                     /note="/label=R3-R N primer"
BASE COUNT      2073 A      1918 C      1816 G      2121 T         0 OTHER
ORIGIN      -
        1 GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT
       61 CTTAGGACGG ATCGCTTGCC TGTAACTTAC ACGCGCCTCG TATCTTTTAA TGATGGAATA
      121 ATTTGGGAAT TTACTCTGTG TTTATTTATT TTTATGTTTT GTATTTGGAT TTTAGAAAGT
      181 AAATAAAGAA GGTAGAAGAG TTACGGAATG AAGAAAAAAA AATAAACAAA GGTTTAAAAA
      241 ATTTCAACAA AAAGCGTACT TTACATATAT ATTTATTAGA CAAGAAAAGC AGATTAAATA
      301 GATATACATT CGATTAACGA TAAGTAAAAT GTAAAATCAC AGGATTTTCG TGTGTGGTCT
      361 TCTACACAGA CAAGATGAAA CAATTCGGCA TTAATACCTG AGAGCAGGAA GAGCAAGATA
      421 AAAGGTAGTA TTTGTTGGCG ATCCCCCTAG AGTCTTTTAC ATCTTCGGAA AACAAAAACT
      481 ATTTTTTCTT TAATTTCTTT TTTTACTTTC TATTTTTAAT TTATATATTT ATATTAAAAA
      541 ATTTAAATTA TAATTATTTT TATAGCACGT GATGAAAAGG ACCCAGGTGG CACTTTTCGG
      601 GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG
      661 CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT
      721 ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT
      781 GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG
      841 GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA
      901 CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT
      961 GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG
     1021 TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT
     1081 GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA
     1141 CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT
     1201 TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA
     1261 GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG
     1321 CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC
     1381 CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT
     1441 ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG
     1501 GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG
     1561 ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA
     1621 CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA
     1681 ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA
     1741 TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG
     1801 CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT
     1861 GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC
     1921 CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG
     1981 GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG
     2041 GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA
     2101 ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC
     2161 GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG
     2221 AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC
     2281 TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC
     2341 AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT
     2401 CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC
     2461 GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC
     2521 CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC
     2581 AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTACCTCACT
     2641 CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC CTATGTTGTG TGGAATTGTG
     2701 AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA GCGCGCAATT
     2761 AACCCTCACT AAAGGGAACA AAAGCTGGAG CTCCACCGCG GTGGCGGCCG CTCTAGAACT
     2821 AGTGGATCCC CCGATTACGC CAAGCTATCA ACTTTGTATA GAAAAGTTGA ACGAGAAACG
     2881 TAAAATGATA TAAATATCAA TATATTAAAT TAGATTTTGC ATAAAAAACA GACTACATAA
     2941 TACTGTAAAA CACAACATAT CCAGTCACTA TGGTCGACCG ACAGCCTTCC AAATGTTCTT
     3001 CTCAAACGGA ATCGTCGTAT CCAGCCTACT CGCTATTGTC CTCAATGCCG TATTAAATCA
     3061 TAAAAAGAAA TAAGAAAAAG AGGTGCGAGC CTCTTTTTTG TGTGACAAAA TAAAAACATC
     3121 TACCTATTCA TATACGCTAG TGTCATAGTC CTGAAAATCA TCTGCATCAA GAACAATTTC
     3181 ACAACTCTTA TACTTTTCTC TTACAAGTCG TTCGGCTTCA TCTGGATTTT CAGCCTCTAT
     3241 ACTTACTAAA CGTGATAAAG TTTCTGTAAT TTCTACTGTA TCGACCTGCA GACTGGCTGT
     3301 GTATAAGGGA GCCTGACATT TATATTCCCC AGAACATCAG GTTAATGGCG TTTTTGATGT
     3361 CATTTTCGCG GTGGCTGAGA TCAGCCACTT CTTCCCCGAT AACGGAGACC GGCACACTGG
     3421 CCATATCGGT GGTCATCATG CGCCAGCTTT CATCCCCGAT ATGCACCACC GGGTAAAGTT
     3481 CACGGGAGAC TTTATCTGAC AGCAGACGTG CACTGGCCAG GGGGATCACC ATCCGTCGCC
     3541 CGGGCGTGTC AATAATATCA CTCTGTACAT CCACAAACAG ACGATAACGG CTCTCTCTTT
     3601 TATAGGTGTA AACCTTAAAC TGCATTTCAC CAGCCCCTGT TCTCGTCAGC AAAAGAGCCG
     3661 TTCATTTCAA TAAACCGGGC GACCTCAGCC ATCCCTTCCT GATTTTCCGC TTTCCAGCGT
     3721 TCGGCACGCA GACGACGGGC TTCATTCTGC ATGGTTGTGC TTACCAGACC GGAGATATTG
     3781 ACATCATATA TGCCTTGAGC AACTGATAGC TGTCGCTGTC AACTGTCACT GTAATACGCT
     3841 GCTTCATAGC ATACCTCTTT TTGACATACT TCGGGTATAC ATATCAGTAT ATATTCTTAT
     3901 ACCGCAAAAA TCAGCGCGCA AATACGCATA CTGTTATCTG GCTTTTAGTA AGCCGGATCC
     3961 ACGCGGCGTT TACGCCCCCC CTGCCACTCA TCGCAGTACT GTTGTAATTC ATTAAGCATT
     4021 CTGCCGACAT GGAAGCCATC ACAAACGGCA TGATGAACCT GAATCGCCAG CGGCATCAGC
     4081 ACCTTGTCGC CTTGCGTATA ATATTTGCCC ATGGTGAAAA CGGGGGCGAA GAAGTTGTCC
     4141 ATATTGGCCA CGTTTAAATC AAAACTGGTG AAACTCACCC AGGGATTGGC TGAGACGAAA
     4201 AACATATTCT CAATAAACCC TTTAGGGAAA TAGGCCAGGT TTTCACCGTA ACACGCCACA
     4261 TCTTGCGAAT ATATGTGTAG AAACTGCCGG AAATCGTCGT GGTATTCACT CCAGAGCGAT
     4321 GAAAACGTTT CAGTTTGCTC ATGGAAAACG GTGTAACAAG GGTGAACACT ATCCCATATC
     4381 ACCAGCTCAC CGTCTTTCAT TGCCATACGG AATTCCGGAT GAGCATTCAT CAGGCGGGCA
     4441 AGAATGTGAA TAAAGGCCGG ATAAAACTTG TGCTTATTTT TCTTTACGGT CTTTAAAAAG
     4501 GCCGTAATAT CCAGCTGAAC GGTCTGGTTA TAGGTACATT GAGCAACTGA CTGAAATGCC
     4561 TCAAAATGTT CTTTACGATG CCATTGGGAT ATATCAACGG TGGTATATCC AGTGATTTTT
     4621 TTCTCCATTT TAGCTTCCTT AGCTCCTGAA AATCTCGATA ACTCAAAAAA TACGCCCGGT
     4681 AGTGATCTTA TTTCATTATG GTGAAAGTTG GAACCTCTTA CGTGCCGATC AACGTCTCAT
     4741 TTTCGCCAAA AGTTGGCCCA GGGCTTCCCG GTATCAACAG GGACACCAGG ATTTATTTAT
     4801 TCTGCGAAGT GATCTTCCGT CACAGGTATT TATTCGGCGC AAAGTGCGTC GGGTGATGCT
     4861 GCCAACTTAC GGCCGCCATA GTGACTGGAT ATGTTGTGTT TTACAGTATT ATGTAGTCTG
     4921 TTTTTTATGC AAAATCTAAT TTAATATATT GATATTTATA TCATTTTACG TTTCTCGTTC
     4981 AACTTTATTA TACATAGTTG ATAATTCACT GGCCGTCGGG GCTGCAGGAA TTCGATATCA
     5041 AGCTTATCGA TACCGTCGAC CTCGAGTCAT GTAATTAGTT ATGTCACGCT TACATTCACG
     5101 CCCTCCCCCC ACATCCGCTC TAACCGAAAA GGAAGGAGTT AGACAACCTG AAGTCTAGGT
     5161 CCCTATTTAT TTTTTTATAG TTATGTTAGT ATTAAGAACG TTATTTATAT TTCAAATTTT
     5221 TCTTTTTTTT CTGTACAGAC GCGTGTACGC ATGTAACATT ATACTGAAAA CCTTGCTTGA
     5281 GAAGGTTTTG GGACGCTCGA AGGCTTTAAT TTGCGGCCGG TACCCAATTC GCCCTATAGT
     5341 GAGTCGTATT ACGCGCGCTC ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT
     5401 GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC
     5461 GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGCGC
     5521 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC
     5581 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC
     5641 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT
     5701 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG
     5761 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT
     5821 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA
     5881 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT
     5941 AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCCT GATGCGGTAT TTTCTCCTTA
     6001 CGCATCTGTG CGGTATTTCA CACCGCATAG CTAGTGGATC TGATATCATC GATGAATTCG
     6061 AGCTCGTTTT CGACACTGGA TGGCGGCGTT AGTATCGAAT CGACAGCAGT ATAGCGACCA
     6121 GCATTCACAT ACGATTGACG CATGATATTA CTTTCTGCGC ACTTAACTTC GCATCTGGGC
     6181 AGATGATGTC GAGGCGAAAA AAAATATAAA TCACGCTAAC ATTTGATTAA AATAGAACAA
     6241 CTACAATATA AAAAAACTAT ACAAATGACA AGTTCTTGAA AACAAGAATC TTTTTATTGT
     6301 CAGTACTGAT TATTCCTTTG CCCTCGGACG AGTGCTGGGG CGTCGGTTTC CACTATCGGC
     6361 GAGTACTTCT ACACAGCCAT CGGTCCAGAC GGCCGCGCTT CTGCGGGCGA TTTGTGTACG
     6421 CCCGACAGTC CCGGCTCCGG ATCGGACGAT TGCGTCGCAT CGACCCTGCG CCCAAGCTGC
     6481 ATCATCGAAA TTGCCGTCAA CCAAGCTCTG ATAGAGTTGG TCAAGACCAA TGCGGAGCAT
     6541 ATACGCCCGG AGCCGCGGCG ATCCTGCAAG CTCCGGATGC CTCCGCTCGA AGTAGCGCGT
     6601 CTGCTGCTCC ATACAAGCCA ACCACGGCCT CCAGAAGAAG ATGTTGGCGA CCTCGTATTG
     6661 GGAATCCCCG AACATCGCCT CGCTCCAGTC AATGACCGCT GTTATGCGGC CATTGTCCGT
     6721 CAGGACATTG TTGGAGCCGA AATCCGCGTG CACGAGGTGC CGGACTTCGG GGCAGTCCTC
     6781 GGCCCAAAGC ATCAGCTCAT CGAGAGCCTG CGCGACGGAC GCACTGACGG TGTCGTCCAT
     6841 CACAGTTTGC CAGTGATACA CATGGGGATC AGCAATCGCG CATATGAAAT CACGCCATGT
     6901 AGTGTATTGA CCGATTCCTT GCGGTCCGAA TGGGCCGAAC CCGCTCGTCT GGCTAAGATC
     6961 GGCCGCAGCG ATCGCATCCA TGGCCTCCGC GACCGGCTGC AGAACAGCGG GCAGTTCGGT
     7021 TTCAGGCAGG TCTTGCAACG TGACACCCTG TGCACGGCGG GAGATGCAAT AGGTCAGGCT
     7081 CTCGCTGAAT TCCCCAATGT CAAGCACTTC CGGAATCGGG AGCGCGGCCG ATGCAAAGTG
     7141 CCGATAAACA TAACGATCTT TGTAGAAACC ATCGGCGCAG CTATTTACCC GCAGGACATA
     7201 TCCACGCCCT CCTACATCGA AGCTGAAAGC ACGAGATTCT TCGCCCTCCG AGAGCTGCAT
     7261 CAGGTCGGAG ACGCTGTCGA ACTTTTCGAT CAGAAACTTC TCGACAGACG TCGCGGTGAG
     7321 TTCAGGCTTT TTACCCATAT GGTTGTTTAT GTTCGGATGT GATGTGAGAA CTGTATCCTA
     7381 GCAAGATTTT AAAAGGAAGT ATATGAAAGA AGAACCTCAG TGGCAAATCC TAACCTTTTA
     7441 TATTTCTCTA CAGGGGCGCG GCGTGGGGAC AATTCAACGC GTCTGTGAGG GGAGCGTTTC
     7501 CCTGCTCGCA GGTCTGCAGC GAGGAGCCGT AATTTTTGCT TCGCGCCGTG CGGCCATCAA
     7561 AATGTATGGA TGCAAATGAT TATACATGGG GATGTATGGG CTAAATGTAC GGGCGACAGT
     7621 CACATCATGC CCCTGAGCTG CGCACGTCAA GACTGTCAAG GAGGGTATTC TGGGCCTCCA
     7681 TGTCGCTGGC CGGGTGACCC GGCGGGGACA AGGCAAGCTA AACAGATCTT GAAAAGCTGT
     7741 GGTATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGCCCCGACA
     7801 CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG
     7861 ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA
     7921 ACGCGCGA
//
