LOCUS       pRS426	5726             8299 bp ss-DNA     linear   SYN 06-NOV-2011
DEFINITION  pRS426
ACCESSION    
KEYWORDS    -
SOURCE      -
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          join(complement(1..>3660),complement(<5847..>5889),
                     complement(<8099..8296))
                     /organism=pRS426
                     /mol_type=other DNA [Split]
     misc_feature    401..448
                     /label=FRT
     rep_origin      440..1411
                     /label=2micron2_origin
     promoter        1473..1501
                     /label=AmpR_promoter
     CDS             1543..2403
                     /label=Ampicillin
                     /gene=Ampicillin
                     /note=ORF frame 3
                     /translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*
     gene            1543..2403
                     /label=Ampicillin
                     /gene=Ampicillin
     rep_origin      2558..3177
                     /label=pBR322_origin
     misc_feature    3529..3551
                     /label=M13_pUC_rev_primer
     misc_feature    3550..3568
                     /label=M13_reverse_primer
     promoter        3585..3604
                     /label=T3_promoter
     misc_feature    3640..3656
                     /label=pBluescriptSK_primer
     primer_bind     3661..3680
                     /label=R4-F N primer
     frag            complement(3661..5846)
                     /note=1 to 2186 of 3-F GW cassette R4-R3
     frag            complement(3661..5846)
                     /note=2067 to 4252 of pRS416 3-frag GW P4-P3r ccDB
     frag            complement(3661..5846)
                     /note=1 to 2186 of Untitled 9
     frag            3661..5846
                     /note=2067 to 4252 of pRS416 3-frag GW P4-P3r ccDB
     frag            3661..5846
                     /note=21 to 2206 of pDEST R4-R3 Vector II
     primer_bind     5825..5846
                     /label=R3-R N primer
     misc_feature    complement(5877..>5889)
                     /label=pBluescriptKS_primer
     frag            5890..8094
                     /note=5062 to 7266 of p41Nat 3-frag GW ccDB
     frag            5890..8094
                     /note=5189 to 7393 of pAG41NAT-GPD-ccdB
     misc_feature    complement(5890..>5893)
                     /label=pBluescriptKS_primer [Split]
     source          <5890..>8094
                     /note=dnas_title=pAG41NAT-GPD-ccdB [Split]
     terminator      5902..6141
                     /label=CYC1_terminator
     misc_feature    complement(5911..5929)
                     /label=CYC1_primer
     misc_feature    complement(5911..5929)
                     /label=pYESTrp_rev_primer
     promoter        complement(6161..6179)
                     /label=T7_promoter
     misc_feature    complement(6189..6205)
                     /label=M13_forward20_primer
     misc_feature    complement(6198..6220)
                     /label=M13_pUC_fwd_primer
     misc_feature    6198..6341
                     /label=lacZ_a
     rep_origin      6367..6673
                     /label=f1_origin
     terminator      complement(6898..7137)
                     /gene=TEF-TER
                     /product=TEF-TER
     CDS             complement(7138..7710)
                     /gene=nat1
                     /product=Nourseothricin acetyltransferase
     promoter        complement(7709..8089)
                     /gene=TEF-PRO
                     /product=TEF-PRO
     frag            <8095..8098
                     /note=5067 to 7516 of p41Neo 3-frag GW ccDB
     frag            <8095..8098
                     /note=5067 to 7516 of Untitled
     misc_feature    8246..8268
                     /label=pGEX_3_primer
BASE COUNT      2182 A      2008 C      1904 G      2204 T         0 OTHER
ORIGIN      ?
        1 GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT
       61 CTTAGTATGA TCCAATATCA AAGGAAATGA TAGCATTGAA GGATGAGACT AATCCAATTG
      121 AGGAGTGGCA GCATATAGAA CAGCTAAAGG GTAGTGCTGA AGGAAGCATA CGATACCCCG
      181 CATGGAATGG GATAATATCA CAGGAGGTAC TAGACTACCT TTCATCCTAC ATAAATAGAC
      241 GCATATAAGT ACGCATTTAA GCATAAACAC GCACTATGCC GTTCTTCTCA TGTATATATA
      301 TATACAGGCA ACACGCAGAT ATAGGTGCGA CGTGAACAGT GAGCTGTATG TGCGCAGCTC
      361 GCGTTGCATT TTCGGAAGCG CTCGTTTTCG GAAACGCTTT GAAGTTCCTA TTCCGAAGTT
      421 CCTATTCTCT AGAAAGTATA GGAACTTCAG AGCGCTTTTG AAAACCAAAA GCGCTCTGAA
      481 GACGCACTTT CAAAAAACCA AAAACGCACC GGACTGTAAC GAGCTACTAA AATATTGCGA
      541 ATACCGCTTC CACAAACATT GCTCAAAAGT ATCTCTTTGC TATATATCTC TGTGCTATAT
      601 CCCTATATAA CCTACCCATC CACCTTTCGC TCCTTGAACT TGCATCTAAA CTCGACCTCT
      661 ACATTTTTTA TGTTTATCTC TAGTATTACT CTTTAGACAA AAAAATTGTA GTAAGAACTA
      721 TTCATAGAGT GAATCGAAAA CAATACGAAA ATGTAAACAT TTCCTATACG TAGTATATAG
      781 AGACAAAATA GAAGAAACCG TTCATAATTT TCTGACCAAT GAAGAATCAT CAACGCTATC
      841 ACTTTCTGTT CACAAAGTAT GCGCAATCCA CATCGGTATA GAATATAATC GGGGATGCCT
      901 TTATCTTGAA AAAATGCACC CGCAGCTTCG CTAGTAATCA GTAAACGCGG GAAGTGGAGT
      961 CAGGCTTTTT TTATGGAAGA GAAAATAGAC ACCAAAGTAG CCTTCTTCTA ACCTTAACGG
     1021 ACCTACAGTG CAAAAAGTTA TCAAGAGACT GCATTATAGA GCGCACAAAG GAGAAAAAAA
     1081 GTAATCTAAG ATGCTTTGTT AGAAAAATAG CGCTCTCGGG ATGCATTTTT GTAGAACAAA
     1141 AAAGAAGTAT AGATTCTTTG TTGGTAAAAT AGCGCTCTCG CGTTGCATTT CTGTTCTGTA
     1201 AAAATGCAGC TCAGATTCTT TGTTTGAAAA ATTAGCGCTC TCGCGTTGCA TTTTTGTTTT
     1261 ACAAAAATGA AGCACAGATT CTTCGTTGGT AAAATAGCGC TTTCGCGTTG CATTTCTGTT
     1321 CTGTAAAAAT GCAGCTCAGA TTCTTTGTTT GAAAAATTAG CGCTCTCGCG TTGCATTTTT
     1381 GTTCTACAAA ATGAAGCACA GATGCTTCGT TCAGGTGGCA CTTTTCGGGG AAATGTGCGC
     1441 GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA
     1501 TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC
     1561 CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA
     1621 ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA
     1681 CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG
     1741 ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA
     1801 GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC
     1861 ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC
     1921 ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA
     1981 ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG
     2041 CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA
     2101 ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA
     2161 GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC
     2221 TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA
     2281 CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA
     2341 ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG
     2401 TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA
     2461 TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT
     2521 GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT
     2581 CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG
     2641 GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA
     2701 GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC
     2761 TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT
     2821 GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG
     2881 CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC
     2941 GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG
     3001 GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA
     3061 GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT
     3121 CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC
     3181 TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC
     3241 CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC
     3301 CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA
     3361 CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC
     3421 TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT ACCTCACTCA TTAGGCACCC
     3481 CAGGCTTTAC ACTTTATGCT TCCGGCTCCT ATGTTGTGTG GAATTGTGAG CGGATAACAA
     3541 TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC GCGCAATTAA CCCTCACTAA
     3601 AGGGAACAAA AGCTGGAGCT CCACCGCGGT GGCGGCCGCT CTAGAACTAG TGGATCCCCC
     3661 GATTACGCCA AGCTATCAAC TTTGTATAGA AAAGTTGAAC GAGAAACGTA AAATGATATA
     3721 AATATCAATA TATTAAATTA GATTTTGCAT AAAAAACAGA CTACATAATA CTGTAAAACA
     3781 CAACATATCC AGTCACTATG GTCGACCGAC AGCCTTCCAA ATGTTCTTCT CAAACGGAAT
     3841 CGTCGTATCC AGCCTACTCG CTATTGTCCT CAATGCCGTA TTAAATCATA AAAAGAAATA
     3901 AGAAAAAGAG GTGCGAGCCT CTTTTTTGTG TGACAAAATA AAAACATCTA CCTATTCATA
     3961 TACGCTAGTG TCATAGTCCT GAAAATCATC TGCATCAAGA ACAATTTCAC AACTCTTATA
     4021 CTTTTCTCTT ACAAGTCGTT CGGCTTCATC TGGATTTTCA GCCTCTATAC TTACTAAACG
     4081 TGATAAAGTT TCTGTAATTT CTACTGTATC GACCTGCAGA CTGGCTGTGT ATAAGGGAGC
     4141 CTGACATTTA TATTCCCCAG AACATCAGGT TAATGGCGTT TTTGATGTCA TTTTCGCGGT
     4201 GGCTGAGATC AGCCACTTCT TCCCCGATAA CGGAGACCGG CACACTGGCC ATATCGGTGG
     4261 TCATCATGCG CCAGCTTTCA TCCCCGATAT GCACCACCGG GTAAAGTTCA CGGGAGACTT
     4321 TATCTGACAG CAGACGTGCA CTGGCCAGGG GGATCACCAT CCGTCGCCCG GGCGTGTCAA
     4381 TAATATCACT CTGTACATCC ACAAACAGAC GATAACGGCT CTCTCTTTTA TAGGTGTAAA
     4441 CCTTAAACTG CATTTCACCA GCCCCTGTTC TCGTCAGCAA AAGAGCCGTT CATTTCAATA
     4501 AACCGGGCGA CCTCAGCCAT CCCTTCCTGA TTTTCCGCTT TCCAGCGTTC GGCACGCAGA
     4561 CGACGGGCTT CATTCTGCAT GGTTGTGCTT ACCAGACCGG AGATATTGAC ATCATATATG
     4621 CCTTGAGCAA CTGATAGCTG TCGCTGTCAA CTGTCACTGT AATACGCTGC TTCATAGCAT
     4681 ACCTCTTTTT GACATACTTC GGGTATACAT ATCAGTATAT ATTCTTATAC CGCAAAAATC
     4741 AGCGCGCAAA TACGCATACT GTTATCTGGC TTTTAGTAAG CCGGATCCAC GCGGCGTTTA
     4801 CGCCCCCCCT GCCACTCATC GCAGTACTGT TGTAATTCAT TAAGCATTCT GCCGACATGG
     4861 AAGCCATCAC AAACGGCATG ATGAACCTGA ATCGCCAGCG GCATCAGCAC CTTGTCGCCT
     4921 TGCGTATAAT ATTTGCCCAT GGTGAAAACG GGGGCGAAGA AGTTGTCCAT ATTGGCCACG
     4981 TTTAAATCAA AACTGGTGAA ACTCACCCAG GGATTGGCTG AGACGAAAAA CATATTCTCA
     5041 ATAAACCCTT TAGGGAAATA GGCCAGGTTT TCACCGTAAC ACGCCACATC TTGCGAATAT
     5101 ATGTGTAGAA ACTGCCGGAA ATCGTCGTGG TATTCACTCC AGAGCGATGA AAACGTTTCA
     5161 GTTTGCTCAT GGAAAACGGT GTAACAAGGG TGAACACTAT CCCATATCAC CAGCTCACCG
     5221 TCTTTCATTG CCATACGGAA TTCCGGATGA GCATTCATCA GGCGGGCAAG AATGTGAATA
     5281 AAGGCCGGAT AAAACTTGTG CTTATTTTTC TTTACGGTCT TTAAAAAGGC CGTAATATCC
     5341 AGCTGAACGG TCTGGTTATA GGTACATTGA GCAACTGACT GAAATGCCTC AAAATGTTCT
     5401 TTACGATGCC ATTGGGATAT ATCAACGGTG GTATATCCAG TGATTTTTTT CTCCATTTTA
     5461 GCTTCCTTAG CTCCTGAAAA TCTCGATAAC TCAAAAAATA CGCCCGGTAG TGATCTTATT
     5521 TCATTATGGT GAAAGTTGGA ACCTCTTACG TGCCGATCAA CGTCTCATTT TCGCCAAAAG
     5581 TTGGCCCAGG GCTTCCCGGT ATCAACAGGG ACACCAGGAT TTATTTATTC TGCGAAGTGA
     5641 TCTTCCGTCA CAGGTATTTA TTCGGCGCAA AGTGCGTCGG GTGATGCTGC CAACTTACGG
     5701 CCGCCATAGT GACTGGATAT GTTGTGTTTT ACAGTATTAT GTAGTCTGTT TTTTATGCAA
     5761 AATCTAATTT AATATATTGA TATTTATATC ATTTTACGTT TCTCGTTCAA CTTTATTATA
     5821 CATAGTTGAT AATTCACTGG CCGTCGGGGC TGCAGGAATT CGATATCAAG CTTATCGATA
     5881 CCGTCGACCT CGAGTCATGT AATTAGTTAT GTCACGCTTA CATTCACGCC CTCCCCCCAC
     5941 ATCCGCTCTA ACCGAAAAGG AAGGAGTTAG ACAACCTGAA GTCTAGGTCC CTATTTATTT
     6001 TTTTATAGTT ATGTTAGTAT TAAGAACGTT ATTTATATTT CAAATTTTTC TTTTTTTTCT
     6061 GTACAGACGC GTGTACGCAT GTAACATTAT ACTGAAAACC TTGCTTGAGA AGGTTTTGGG
     6121 ACGCTCGAAG GCTTTAATTT GCGGCCGGTA CCCAATTCGC CCTATAGTGA GTCGTATTAC
     6181 GCGCGCTCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA
     6241 CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC
     6301 ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGCGCGA CGCGCCCTGT
     6361 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC
     6421 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC
     6481 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG
     6541 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA
     6601 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC
     6661 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG
     6721 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT
     6781 AACAAAATAT TAACGTTTAC AATTTCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG
     6841 GTATTTCACA CCGCATAGCT AGTGGATCTG ATATCATCGA TGAATTCGAG CTCGTTTTCG
     6901 ACACTGGATG GCGGCGTTAG TATCGAATCG ACAGCAGTAT AGCGACCAGC ATTCACATAC
     6961 GATTGACGCA TGATATTACT TTCTGCGCAC TTAACTTCGC ATCTGGGCAG ATGATGTCGA
     7021 GGCGAAAAAA AATATAAATC ACGCTAACAT TTGATTAAAA TAGAACAACT ACAATATAAA
     7081 AAAACTATAC AAATGACAAG TTCTTGAAAA CAAGAATCTT TTTATTGTCA GTACTGATTA
     7141 GGGGCAGGGC ATGCTCATGT AGAGCGCCTG CTCGCCGTCC GAGGCGGTGC CGTCGTACAG
     7201 GGCGGTGTCC AGGCCGCAGA GGGTGAACCC CATCCGCCGG TACGCGTGGA TCGCCGGTGC
     7261 GTTGACGTTG GTGACCTCCA GCCAGAGGTG CCCGGCGCCC CGCTCGCGGG CGAACTCCGT
     7321 CGCGAGCCCC ATCAACGCGC GCCCGACCCC GTGCCCCCGG TGCTCCGGGG CGACCTCGAT
     7381 GTCCTCGACG GTCAGCCGGC GGTTCCAGCC GGAGTACGAG ACGACCACGA AGCCCGCCAG
     7441 GTCGCCGTCG TCCCCGTACG CGACGAACGT CCGGGAGTCC GGGTCGCCGT CCTCCCCGTC
     7501 GTCCGATTCG TCGTCCGATT CGTCGTCGGG GAACACCTTG GTCAGGGGCG GGTCCACCGG
     7561 CACCTCCCGC AGGGTGAAGC CGTCCCCGGT GGCGGTGACG CGGAAGACGG TGTCGGTGGT
     7621 GAAGGACCCA TCCAGTGCCT CGATGGCCTC GGCGTCCCCC GGGACACTGG TGCGGTACCG
     7681 GTAAGCCGTG TCGTCAAGAG TGGTACCCAT GGTTGTTTAT GTTCGGATGT GATGTGAGAA
     7741 CTGTATCCTA GCAAGATTTT AAAAGGAAGT ATATGAAAGA AGAACCTCAG TGGCAAATCC
     7801 TAACCTTTTA TATTTCTCTA CAGGGGCGCG GCGTGGGGAC AATTCAACGC GTCTGTGAGG
     7861 GGAGCGTTTC CCTGCTCGCA GGTCTGCAGC GAGGAGCCGT AATTTTTGCT TCGCGCCGTG
     7921 CGGCCATCAA AATGTATGGA TGCAAATGAT TATACATGGG GATGTATGGG CTAAATGTAC
     7981 GGGCGACAGT CACATCATGC CCCTGAGCTG CGCACGTCAA GACTGTCAAG GAGGGTATTC
     8041 TGGGCCTCCA TGTCGCTGGC CGGGTGACCC GGCGGGGACA AGGCAAGCTA AACAAGATCT
     8101 TGAAAAGCTG TGGTATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC
     8161 CAGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA
     8221 TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG
     8281 TCATCACCGA AACGCGCGA
//
