Name type location mut agc --> tcg misc_feature 3076..3078 Pcds misc_feature 3380..3399 N cds misc_feature 108..807,809..1685 Flag-P misc_feature 1807..1809,1834..2067 C cds NB maybe elongated from two ATG upstream & inmisc_feature 1853..2067 F cds misc_feature 5551..7212 H cds misc_feature 7373..9226 L cds misc_feature 9336..15887 Trailer (as) misc_feature 15960..15996 Leader misc_feature 1..41 Flag misc_feature 1810..1833 P cds misc_feature 2068..2922,2943..3075,3079..3318,3321..3333,3335..3354 C cds misc_feature 2068..2413 M-ATG_as primer misc_feature rev:3530..3549 XD misc_feature 3208..3318,3321..3333,3335..3354 M cds misc_feature 3540..4547 mut XD Schwarz GTG --> aTGmisc_feature 3334..3334 mut F497A misc_feature 3319..3320 mut site BsiWI cgtacg --> cgtacCmisc_feature 3467..3467 Amp pro misc_feature 18352..18380 T3 primer_bind rev:16248..16267 M13-fwd primer_bind 19003..19020 M13-rev primer_bind rev:16285..16305 ColE1 origin rep_origin rev:16673..17355 LacZ alpha CDS rev:18864..18932 LacO misc_binding rev:16311..16333 AmpR CDS rev:17453..18112 AmpR promoter misc_feature rev:18352..18380 Lac promoter misc_feature rev:16338..16367 M13rev(-29) MWG seqmisc_feature rev:16288..16305 M13rev(-49) MWG seqmisc_feature rev:16302..16325 M13 uni (-21) misc_feature 19003..19020 M13uni(-43) MWG seq misc_feature 18981..19003 si2(siP) target misc_feature 2923..2942 si2(siP) target misc_feature 3380..3399 si2(siP) target misc_feature 3406..3425 si2(siP) target misc_feature 3432..3451