LOCUS       Tet-On-TAP\Vecto        6803 bp    DNA     circular     12-APR-2010
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|551270122|
COMMENT     VNTDBDATE|551270410|
COMMENT     LSOWNER|
COMMENT     VNTNAME|Tet-On-TAP Vector|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTUDF|Cloned by:|1|Dominique Sch|
COMMENT     VNTUDF|Date glycerol made:|1|1/27/2005|
COMMENT     VNTUDF|Host strain:|1|XL1-Blue MRF'|
COMMENT     VNTUDF|Organism|4|E.coli|
COMMENT     VNTUDF|Freezer Box:|1|WU# 2 - I  6 B|
COMMENT     VNTUDF|Selection:|5|Ampicillin|
FEATURES             Location/Qualifiers
     misc_difference 21..327
                     /vntifkey="85"
                     /label=f1\(-)\ori\(21-327)
     mutation        complement(5818..6675)
                     /vntifkey="62"
                     /label=Amp
     misc_structure  2727..3026
                     /vntifkey="88"
                     /label=TgSAG1\3'UTR\from\1326bp-1625bp(1977-2276)
     primer_bind     626..644
                     /vntifkey="28"
                     /label=T7\promoter\primer\(626-644)
     primer_bind     complement(4614..4633)
                     /vntifkey="28"
                     /label=T3\promoter\primer\(2342-2361)
     primer_bind     complement(2819..2838)
                     /vntifkey="28"
                     /label=pTetO7Sag1mycGFP\3'UTR\Reverse\primer\(2069-2088)
     misc_feature    658..2075
                     /vntifkey="21"
                     /label=RPS12\promoter\with\Tet\Operators
     misc_feature    3051..4566
                     /vntifkey="21"
                     /label=Sag-Ble-Sag\Cassette
     misc_feature    2062..2073
                     /vntifkey="21"
                     /label=Designer\Tozak\\Alanine\as\2nd\codon
     misc_feature    2173..2250
                     /vntifkey="21"
                     /label=CBP-tag
     misc_feature    2305..2718
                     /vntifkey="21"
                     /label=Protein\A\tag
     misc_feature    2073..2104
                     /vntifkey="21"
                     /label=Multiple\Cloning\Site
BASE COUNT     1708 a      1766 c      1649 g      1680 t 
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg 
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccag 
      661 cttgtcagtg catgacacaa ccgcgaaaag ccggcagccg cggtaatacg gggacgagga 
      721 aaacgactga gcgtcacaac agaagcagcc gagtaaacgg cgaaggaaat ggaaaggacc 
      781 caagtaaaat ttcttgaaga atttcagcgc aacaactctg cgggttcttg cgaatagagg 
      841 aatttcactt cctcatcgtc tgatttatgc tttcatcatc tgccgctcaa cagccgaata 
      901 aacggttctc ggtcgcttcc ttaaactcta cttcagtagt tgaaactctt ttgcttcacg 
      961 agccttcgtc tcagccctca ccgtcctgag ttctgtcttt gttgaggaaa gctcccgctg 
     1021 aaaaaacagg actttgtttg cagattttca tgtgtactgg aaagtgagat gtgacttggg 
     1081 gaagtccgct ttaaaatttc cattgttttc tcaaaatgaa aagtctaaaa aatcgaagtg 
     1141 cgtgccccgc gaggaattcc cctctgcaga tttgttttgc atttatatgt cgtttttacg 
     1201 gagaaaagtc ccaagctgct gctccttctc taactagatg ttgaacgcta gcacatatgc 
     1261 accagatgct tctgaagtat acctaaacgc accttgggaa caactgtgct cccattcata 
     1321 aaactcatac aagtcaccaa gcatgccata cccgtgagac ataacaacgg aagctagact 
     1381 actcccccct gttattgcac actatcgaaa aggattcgta ggtttctatc ctctgccttt 
     1441 tcctggggca cactgcagag aaactaccgt gcgcgctacc tcccgacgtg cgaggcgata 
     1501 gcaaaacgct tttgaaggaa aaagtcgaga aatcgacgac tgcgtctctt gaatccgaga 
     1561 gaggcatcca acccaccgag ttctctgcat gtgcagcatc tgcaagaacg tgataatgca 
     1621 tgaactcgat catcgcctta tctgtgtgca tgcattttcg aaaaagaaag gcgttttctg 
     1681 cgcggagact cgcgcggagg caagacgaga ctttctcctc ttccaaactg cgagccacgg 
     1741 gggcgcatgc aatttgaaca tcacgcaaaa tcccaaaacg ggtggggtgg agccgcaaac 
     1801 ttttttggca tgcagcgttg agcctgagct gcggtggggg cttttgtcgc gagcgtgggg 
     1861 tgccgcgaga gagcaacgcg gcgctacgcg gccgacggtc cctatcagtg atagagatct 
     1921 ccctatcagt gatagagatc tctagttccc tatcagtgat agagatctcc ctatcagtga 
     1981 tagagatctg tagattcctc gacgggttct cccctcaatt ctcttccttt ctctgcgtct 
     2041 tcctcaggtg gcttcgtcac cgtcaaaatg gcgcaattgc ctaggaagct tctcgaggga 
     2101 tccatggaac agaaactgat ctctgaagaa gacctgcacc atcatcacca ccatcaccat 
     2161 tctagaatgg aaaagagaag atggaaaaag aatttcatag ccgtctcagc agccaaccgc 
     2221 tttaagaaaa tctcatcctc cggggcactt gattatgata ttccaactac tgctagcgag 
     2281 aatttgtatt ttcagggtga gctcaaaacc gcggctcttg cgcaacacga tgaagccgtg 
     2341 gacaacaaat tcaacaaaga acaacaaaac gcgttctatg agatcttaca tttacctaac 
     2401 ttaaacgaag aacaacgaaa cgccttcatc caaagtttaa aagatgaccc aagccaaagc 
     2461 gctaaccttt tagcagaagc taaaaagcta aatgatgctc aggcgccgaa agtagacaac 
     2521 aaattcaaca aagaacaaca aaacgcgttc tatgagatct tacatttacc taacttaaac 
     2581 gaagaacaac gaaacgcctt catccaaagt ttaaaagatg acccaagcca aagcgctaac 
     2641 cttttagcag aagctaaaaa gctaaatggt gctcaggcgc cgaaagtaga cgcgaattcc 
     2701 gcggggaagt caacctgatt aattaatcac cgttgtgctc acttctcaaa tcgacaaagg 
     2761 aaacacactt cgtgcagcat gtgccccatt ataaagaaac tgagttgttc cgttgtggct 
     2821 tgcaggtgtc acatccacaa aaaccggccg actctaaata ggagtgtttc gcagcaagca 
     2881 gcgaaagttt atgactgggt ccgaatctct gaacggatgt gtggcggacc tggctgatgt 
     2941 tgatcgccgt cgacacacgc gccacatggg tcaatacaca agacagctat cagttgtttt 
     3001 agtcgaaccg gttaacacaa ttcttgcccc cccgaggggc atccactagt gctcttcaag 
     3061 gttttacatc cgttgccttt tccacggtcc gtgatttcat gtgcgtgcag cttcaaagac 
     3121 tggtcgttgc gactaataag actgcagtga caggtcgaat ggtgggcacc ttgctgatga 
     3181 ctatctactg caaagtctga gacaacgaac gaaacttccc acacgaggca tttgaaactg 
     3241 acggtgtcta ggtaatatgc actgcaagac acggtactgg ggcctcgctg aattaggggc 
     3301 cgatctcgtt gccctatcag tgctcacagt gccgcaacgt aacaccaggg caggttcttg 
     3361 acagtggcaa caatgtgcga cgggcgtgtg aacgtttcgt agtcatagcg ctagcacgta 
     3421 cctagccaca tggtcgtgag gagctttacc atgcgtctac aaggtggatg cgggacacgc 
     3481 cttcctggcc tttggctccc gagacgcgtg ttctaaccac aaaccttgag acgcgtgttc 
     3541 caaccacgca ccctgacacg cgtgttccaa ccacgcaccc tgagacgcgt gttctaacca 
     3601 cgcaccctga gacgcgtgtt ctaaccacgc accctgagac gcgtgttctg ccgcacaatg 
     3661 tgcacctgta ggaagctgta gtcactgctg attctcactg ttctcggcaa gggccgacga 
     3721 ccggagtaca gtttttgtgg gcagagccgt tgtgcagctt tccgttcttc tcggttgtgt 
     3781 cacatgtgtc attgtcgtgt aaacacacgg ttgtatgtcg gtttcgctgc accacttcat 
     3841 tatttcttct ggttttttga cgagtatgca tgaccaagcg acgcccaacc tgccatcacg 
     3901 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga 
     3961 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccgg 
     4021 gctcgatccc ctcgcgagtt ggttcagctg ctgcctgagg ctggacgacc tcgcggagtt 
     4081 ctaccggcag tgcaaatccg tcggcatcca ggaaaccagc agcggctatc cgcgcatcca 
     4141 tgcccccgaa ctgcaggagt ggggaggcac gatggccgct ttggtcgacc cggacgggac 
     4201 gctcctgcgc ctgatacaga acgaattgct tgcaggcatc tcttaattaa tcaccgttgt 
     4261 gctcacttct caaatcgaca aaggaaacac acttcgtgca gcatgtgccc cattataaag 
     4321 aaactgagtt gttccgctgt ggcttgcagg tgtcacatcc acaaaaaccg gccgactcta 
     4381 aataggagtg tttcgcagca agcagcgaaa gtttatgact gggtccgaat ctctgaacgg 
     4441 atgtgtggcg gacctggctg atgttgatcg ccgtcgacac acgcgccaca tgggtcaata 
     4501 cacaagacag ctatcagttg ttttagtcga accggttaac acaattcttg cccccccgag 
     4561 ctcttcacta gttgtagagc ggccgccacc gcggtggagc tccagctttt gttcccttta 
     4621 gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 
     4681 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 
     4741 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 
     4801 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 
     4861 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 
     4921 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 
     4981 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 
     5041 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 
     5101 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 
     5161 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 
     5221 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 
     5281 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 
     5341 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 
     5401 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 
     5461 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 
     5521 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 
     5581 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 
     5641 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 
     5701 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 
     5761 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 
     5821 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 
     5881 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 
     5941 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 
     6001 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 
     6061 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 
     6121 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 
     6181 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 
     6241 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 
     6301 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 
     6361 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 
     6421 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 
     6481 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 
     6541 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 
     6601 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 
     6661 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 
     6721 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 
     6781 cacatttccc cgaaaagtgc cac 
//
