LOCUS       pICE-FLAG-NAT10-siR-WT        8086 bp    DNA     circular     21-JUL-2014
SOURCE      
  ORGANISM  
FEATURES             Location/Qualifiers
     misc_feature    4263..4865
                     /vntifkey="21"
                     /label=PuroR
     rep_origin      complement(5509..6179)
                     /invitrogen="2540000"
                     /vntifkey="33"
                     /label=pUC\origin
     promoter        complement(7185..7283)
                     /invitrogen="2330000"
                     /vntifkey="30"
                     /label=bla\promoter
     CDS             complement(6324..7184)
                     /invitrogen="1020000"
                     /vntifkey="4"
                     /label=Amp(R)
     misc_feature    7360..8084
                     /vntifkey="21"
                     /label=CMVTET
     misc_feature    7946..7985
                     /vntifkey="21"
                     /label=TetO
     polyA_site      3173..3397
                     /invitrogen="1870000"
                     /vntifkey="26"
                     /label=BGH\pA
     rep_origin      3443..3871
                     /invitrogen="2500000"
                     /vntifkey="33"
                     /label=f1\origin
     promoter        3876..4245
                     /invitrogen="2290000"
                     /vntifkey="29"
                     /label=SV40\early\promoter
     polyA_signal    4995..5125
                     /invitrogen="1840000"
                     /vntifkey="25"
                     /label=SV40\pA
     misc_feature    55..3132
                     /vntifkey="21"
                     /label=NAT10
     misc_feature    2920..2946
                     /vntifkey="21"
                     /label=siRNA-resistant\mutations
     misc_feature    22..48
                     /vntifkey="21"
                     /label=FLAG
BASE COUNT     1931 a      2131 c      2085 g      1939 t 
ORIGIN
        1 aagcttgcgg ccgccgccac catggattac aaggatgacg acgataaggg atccatgcat 
       61 cggaaaaagg tggataaccg aatccggatt ctcattgaga atggagtagc tgagcggcaa 
      121 agatctctct ttgttgtagt tggggatcga ggaaaagatc aggtggtaat acttcatcac 
      181 atgttatcca aagcaactgt gaaggctcgg ccttcagtgc tgtggtgtta taagaaagag 
      241 ctggggttta gcagtcaccg gaagaaaaga atgcgacagc tgcagaagaa aataaagaat 
      301 ggaacactga acataaagca ggacgacccc tttgaactct tcatagcagc cacaaacatt 
      361 cgctactgct actacaacga gacccacaag atcctgggca ataccttcgg catgtgtgtg 
      421 ctgcaggatt ttgaagcctt aactccaaac ttgctggcca ggactgtaga aacagtggaa 
      481 ggtggtgggc tagtggtcat cctcctacgg accatgaact cactcaagca attgtacaca 
      541 gtgactatgg atgtgcattc caggtacaga actgaggccc atcaggatgt ggtgggaaga 
      601 tttaatgaaa ggtttattct gtctctggcc tcttgtaaga agtgtctcgt cattgatgac 
      661 cagctcaaca tcctgcccat ctcctcccac gttgccacca tggaggccct gcctccccag 
      721 actccggatg agagtcttgg tccttctgat ctggagctga gggagttgaa ggagagcttg 
      781 caggacaccc agcctgtggg tgtgttggtg gactgctgta agactctaga ccaggccaaa 
      841 gctgtcttga aatttatcga gggcatctct gaaaagaccc tgaggagtac tgttgcactc 
      901 acagctgctc gaggacgggg aaaatctgca gccctgggat tggcgattgc tggggcggtg 
      961 gcatttgggt actccaatat ctttgttacc tccccaagcc ctgataacct ccatactctg 
     1021 tttgaatttg tatttaaagg atttgatgct ctgcaatatc aggaacatct ggattatgag 
     1081 attatccagt ctctaaatcc tgaatttaac aaagcagtga tcagagtgaa tgtatttcga 
     1141 gaacacaggc agactattca gtatatacat cctgcagatg ctgtgaagct gggccaggct 
     1201 gaactagttg tgattgatga agctgccgcc atccccctcc ccttggtgaa gagcctactt 
     1261 ggcccctacc ttgttttcat ggcatccacc atcaatggct atgagggcac tggccggtca 
     1321 ctgtccctca agctaattca gcagctccgt caacagagcg cccagagcca ggtcagcacc 
     1381 actgctgaga ataagaccac gacgacagcc agattggcat cagcgcggac actgcatgag 
     1441 gtttccctcc aggagtcaat ccgatacgcc cctggggatg cagtggagaa gtggctgaat 
     1501 gacttgctgt gcctggattg cctcaacatc actcggatag tctcaggctg ccccttgcct 
     1561 gaagcttgtg aactgtacta tgttaataga gataccctct tttgctacca caaggcctct 
     1621 gaagttttcc tccaacggct tatggccctc tacgtggctt ctcactacaa gaactctccc 
     1681 aatgatctcc agatgctctc cgatgcacct gctcaccatc tcttctgcct tctgcctcct 
     1741 gtgcccccca cccagaatgc ccttccagaa gtgcttgctg ttatccaggt gtgccttgaa 
     1801 ggggagattt ctcgccagtc catcttgaac agtctgtctc gaggcaagaa ggcttcaggg 
     1861 gacctgattc catggacagt gtcagaacag ttccaagatc cagactttgg tggtctgtct 
     1921 ggtggaaggg tcgttcgcat tgctgttcac ccagattatc aagggatggg ctatggcagc 
     1981 cgtgctctgc agctgctgca gatgtactat gaaggcaggt ttccttgtct ggaggaaaag 
     2041 gtccttgaga caccacagga aattcacacc gtaagcagcg aggctgtcag cttgttggaa 
     2101 gaggtcatca ctccccggaa ggacctgcct cctttactcc tcaaattgaa tgagaggcct 
     2161 gccgaacgcc tggattacct gggtgtttcc tatggcttga cccccaggct cctcaagttc 
     2221 tggaaacgag ctggatttgt tcctgtttat ctgagacaga ccccgaatga cctgaccgga 
     2281 gagcactcgt gcatcatgct gaagacgctc actgatgagg atgaggctga ccagggaggc 
     2341 tggcttgcag ccttctggaa agatttccga cggcggttcc tagccttgct ctcctaccag 
     2401 ttcagtacct tctctccttc cctggctctg aacatcattc agaacaggaa catggggaag 
     2461 ccagcccagc ctgccctgag ccgggaggag ctggaagcac tcttcctccc ctatgacctg 
     2521 aagcggctgg agatgtattc acggaatatg gtggactatc acctcatcat ggacatgatc 
     2581 ccggccatct ctcgcatcta tttcctgaac cagctggggg acctggccct gtctgcggct 
     2641 cagtcggctc ttctcttggg gattggcctg cagcataagt ctgtggacca gctggaaaag 
     2701 gagattgagc tgccctcggg ccagttgatg ggacttttca accggatcat ccgcaaagtt 
     2761 gtgaagctat ttaatgaagt tcaggaaaag gccattgagg agcagatggt ggcagcgaag 
     2821 gatgtggtca tggagcccac gatgaagacc ctcagtgacg acctagatga agcagcaaag 
     2881 gaatttcagg agaaacacaa gaaggaagta gggaagctga aatcaatgga tttgagtgaa 
     2941 tatattatcc gtggggacga tgaagagtgg aatgaagttt tgaacaaagc tgggccgaac 
     3001 gcctcgatca tcagcctgaa aagtgacaag aaaaggaagt tagaggccaa acaagaaccc 
     3061 aaacagagca agaagttgaa gaacagagag acaaagaaca aaaaagatat gaaactgaag 
     3121 cggaagaaat agacgcgtct gcagtctaga taaacccgct gatcagcctc gactgtgcct 
     3181 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt 
     3241 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg 
     3301 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac 
     3361 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc 
     3421 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg 
     3481 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct 
     3541 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg 
     3601 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag 
     3661 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg 
     3721 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc 
     3781 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat 
     3841 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt 
     3901 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 
     3961 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 
     4021 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc 
     4081 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 
     4141 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 
     4201 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg 
     4261 tgaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg 
     4321 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc 
     4381 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc 
     4441 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc 
     4501 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga 
     4561 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca 
     4621 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc 
     4681 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct 
     4741 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca 
     4801 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg 
     4861 cctgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg 
     4921 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt 
     4981 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 
     5041 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 
     5101 tcatcaatgt atcttatcat gtctggctag ccgtcgacct ctagctagag cttggcgtaa 
     5161 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 
     5221 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 
     5281 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa 
     5341 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 
     5401 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 
     5461 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 
     5521 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 
     5581 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 
     5641 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 
     5701 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 
     5761 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 
     5821 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 
     5881 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 
     5941 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 
     6001 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 
     6061 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtttttt tgtttgcaag 
     6121 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 
     6181 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 
     6241 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 
     6301 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 
     6361 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 
     6421 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 
     6481 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 
     6541 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 
     6601 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 
     6661 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 
     6721 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 
     6781 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 
     6841 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 
     6901 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 
     6961 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 
     7021 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 
     7081 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 
     7141 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 
     7201 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 
     7261 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 
     7321 gacggatcgg gagatcctgt acgggccaga tatccgcgtt gacattgatt attgactagt 
     7381 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt 
     7441 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg 
     7501 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg 
     7561 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt 
     7621 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg 
     7681 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg 
     7741 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt 
     7801 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac 
     7861 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg 
     7921 tgggaggtct atataagcag agctctccct atcagtgata gagatctccc tatcagtgat 
     7981 agagatcgtc gacgagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc 
     8041 tgttttgacc tccatagaag acaccgggac cgatccagcc tccggc 
//
